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Figure 3 | BMC Neuroscience

Figure 3

From: Period 2 regulates neural stem/progenitor cell proliferation in the adult hippocampus

Figure 3

The mutation of the m Per2 gene increases the number of proliferating NPCs in the adult dentategyrus. (A-B) Images displaying Ki67+ cells (A-B) and BrdU+ cells (D-E) (one i.p. injection at 100 mg/kg of body weight and sacrifice 2 hours later) in the DG from P45 WT (A and D) and Per2Brdm1(B and E) mice. TO-PRO®-3 was used for nuclear counterstaining. All images represent «Z-stack» overlays on 40 μm-thick coronal sections of 5 adjacent confocal planes (step size = 1 μm). (C and F) Histograms representing an unbiased assessment of the number of Ki67+ (C) and BrdU+ (F) cells (mean ± SD, n = 7, Student's t-test, ***p < 0,001, *p < 0,05). Scale bar in A = 50 μm for A-B; D-E.

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