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Figure 2 | BMC Neuroscience

Figure 2

From: Period 2 regulates neural stem/progenitor cell proliferation in the adult hippocampus

Figure 2

Phenotype of mPER2-expressing cells in the adult dentate gyrus. (A-D) Confocal images showing some undifferentiated dividing Sox2+ (B) and Ki67+ (C) cells located in the SGL expressing mPER2 (D). (E-H) mPer2 mRNA distribution in the GCL revealed by in situ hybridization (E) and immunostained for BrdU (F) (one i.p. injection at 100 mg/kg of body weight) and Ki67 (G). (H) DAPI was used for nuclear counterstaining. Insets show a mPER2+ cell also labelled for BrdU (F) and Ki67 (G). (I-J) Immature neural precursor DCX+ were found to be mPER2+ (J, white arrows). (I and K) All the mature neurons expressing NeuN were found to be mPER2+ (yellow arrowhead), white arrows point towards mPER2+/NeuN- cells. (A-K) All images represent «Z-stack» overlays from 40 μm-thick coronal sections of 5 adjacent confocal planes (step size = 1 μm). (L) Schematic representation of the mPER2 expression in mitotic and post-mitotic cells of the DG. Scale bar in A = 30 μm for A-D and I-K, 50 μm for E-H.

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