Imaging cytoplasmic cAMP in mouse brainstem neurons

BMC Neuroscience

Table 2 Mean peak [Ca²⁺]_i and [cAMP]_i increases due to membrane depolarisation and Ca²⁺ release applications in the presence of drugs modulating cAMP and Ca²⁺ homeostasis.

	Intracellular variable	Control	IBMX 50 μM	DDA 100 μM	Thapsigargin 2 μM	0 Ca²⁺
High-K⁺ 50 mM	[Ca²⁺]_i	0.44 ± 0.07	0.72 ± 0.08	0.24 ± 0.06	0.31 ± 0.05	0.01 ± 0.01
	[cAMP]_i	1.52 ± 0.09	3.22 ± 0.17	0.25 ± 0.02	0.94 ± 0.08	0.04 ± 0.01
ATP 1 mM	[Ca²⁺]_i	0.39 ± 0.07	0.52 ± 0.08	0.24 ± 0.06	0.01 ± 0.01	0.31 ± 0.05
	[cAMP]_i	1.44 ± 0.08	2.24 ± 0.07	0.36 ± 0.03	0.04 ± 0.01	1.42 ± 0.08

Mean data ± S. E. M. were obtained in 4 to 8 neurons in four different preparations. Thapsigargin and Ca²⁺-free solution were applied for 10 min before the treatments.

ISSN: 1471-2202