Figure 2

Micrographs showing multiple-labelled tissue of the rat, macaque and human brain (A-F). (A) Split image displaying labelling of YB-1 (red) and NeuN (green) and double-labelling (merged figure) in the hippocampal cornu ammonis (CA-1) formation of a rat brain. Representative examples of cells showing nuclear YB-1 expression are indicated by bold white arrowheads while a narrow arrow indicates a cell with cytoplasmatic expression of YB-1. (B) High magnification image of a neuron indicated by NeuN expression (green) with a clear nuclear staining of YB-1 (red) in the parietal cortex of a macaque. (C) Split image showing the expression of YB-1 (red) and the glial marker GFAP (green) in the hilus of the dentate gyrus of a rat. Note the absence of any glial expression of YB-1 in the merged figure. (D, E) Confocal image showing the expression of YB-1 (red) and the expression of the endothelial cell marker Glut-1 (green) in the macaque and human brain. (D) Image displaying the expression of YB-1 (red) and Glut-1 (green) in the parahippocampal cortex of a macaque monkey. Note the lack of co-localization of YB-1 and Glut-1 expression, as clearly indicated by confocal z-series. (E) Image displaying the expression of YB-1 (red) and Glut-1 (green) in the human parahippocampal cortex. In accordance with data obtained from rats (data not shown) and macaques, YB-1 proved not to be expressed by Glut-1 positive endothelial cells. (F) Split image of a confocal micrograph giving an example of YB-1 expression (red) and expression of the endothelial multidrug transporter Pgp (green) in the parietal cortex of a rat. In accordance with the data from the YB-1/Glut-1 double-labelling in the different species, YB-1 does not co-localize with endothelial Pgp expression (merged figure). Scale bar = 10 μm.