Figure 6

SCN2A is expressed in TRPM5 and PKD2L1 cells. A-C and G-I, Double label IHC for TRPM5 (A, G) and SCN2A (B, H) demonstrating SCN2A immunoreactivity in TRPM5 cells. TRPM5 signals are associated with SCN2A signals; however, many SCN2A cells do not express TRPM5 in the merged images (C, I). D-F and J-L, Double label ISH for PKD2L1 (D, J) and SCN2A (E, K) demonstrating SCN2A immunoreactivity in PKD2L1 cells. PKD2L1 signals are associated with SCN2A signals; however, many SCN2A cells do not express PKD2L1 in the merged images (F, L). M-N, Double label IHC for TRPM5 with PKD2L1 (M) and SCN2A (N) demonstrating SCN2A immunoreactivity in TRPM5 and PKD2L1 cells. Combined TRPM5 and PKD2L1 signals are associated with SCN2A signals in the merged image (O). Quantitation of SCN2A expression in TRPM5 and PKD2L1 cells (P). Images in A-F and M-O illustrate multiple taste buds; images in G-L illustrate single taste buds with the taste pore at the top. Images are from CV papilla and are longitudinal sections (A-L) or transverse sections (M-O). Scale bar is 40 μm in F and represents scale for A-F, 20 μm in L and represents scale for G-L, and 50 μm in O and represents scale for M-O.