SCN9A is expressed in TRPM5 but not PKD2L1 cells. A-C and G-I, Double label IHC for TRPM5 (A, G) and SCN9A (B, H) demonstrating SCN9A immunoreactivity in TRPM5 cells. TRPM5 and SCN9A signals label similar cells in the merged images (C, I). D-F and J-L, Double label IHC for PKD2L1 (D, J) and SCN9A (E, K) demonstrating SCN9A immunoreactivity is absent from PKD2L1 cells. PKD2L1 and SCN9A signals label distinct cells in the merged images (F, L). Quantitation of SCN9A expression in TRPM5 cells (M) and PKD2L1 cells (N). Images are from CV papilla and are longitudinal sections. Images in A-F illustrate multiple taste buds; images in G-L illustrate single taste buds with the taste pore at the top. Scale bar is 30 μm in F and represents scale for A-F, and 15 μm in I and represents scale for G-L.