Table 2 Sequences of primer pairs and probes used for the real-time RT-PCR
Gene GenBank Accession # | Sequence 5'-3' | Fragment size (bp) |
|---|---|---|
α rENaC (NM_031548) |  | 96 |
Forward primer | GAGTCTCCTTCTGTCACGATGG | Â |
Reverse primer | CATCTCCACCACAGAGAGCAC | Â |
TaqMan probe | CCAAACCACAGGCTCCACTGGCTGC | Â |
β rENaC (x77932) |  | 99 |
Forward primer | CCAATGGGACCGTGTGTACC | Â |
Reverse primer | GCACTTGTGAGAAGATGTTGGTG | Â |
TaqMan probe | CCGAAACTTCACCAGTGCCACCCAGG | Â |
γ rENaC (NM_017046) |  | 106 |
Forward primer | AGCCAAGGTGCTTATCCATCAG | Â |
Reverse primer | TTCTGTCAGGTGCATTCCTATGG | Â |
TaqMan probe | CATTGCTGTCTCGATCTCCATCCCCACG | Â |
rBDNF (NM_012513) | Â | 97 |
Forward primer | TCAGCAGTCAAGTGCCTTTGG | Â |
Reverse primer | CGCCGAACCCTCATAGACATG | Â |
TaqMan probe | CCTCCTCTGCTCTTTCTGCTGGAGGAATACAA | Â |
rTrkB (M55292) | Â | 100 |
Forward primer | TTTGTGGCTTACAAGGCGTTTC | Â |
Reverse primer | GGTGGCGGAAATGTCTCCTG | Â |
TaqMan probe | AAGAACGGCAACCTGCGGCACATCAA | Â |