Normalized expression of α, β and γ ENaC mRNA in the rat distal colon. Expression levels were normalized to each sample's respective GAPDH expression and then reported as a fold of control (mean ± S.E.M). The data of control were from rats fed with a diet containing 1% NaCl, and the data of the deprivation were from those fed with a diet containing 0.03% NaCl for 15 days. n indicates the number of animals used. Next figures are the same. *** p < 0.001.