ChAT+ neurons in the adult murine cerebral cortex. A: laminar distribution of cholinergic cells in the somatosensory cortex (ss), where they appear to be widely distributed in the different layers, but more concentrated in II-III and lying sometimes in proximity of vessel (v) wall. ChAT+ cells display fusiform bipolar (arrows) or round multipolar (arrowheads) shapes throughout the cortical mantle (B, C, D, E). B, C: in the visual cortex (vis, B) cholinergic cells show the same distribution and density observed in the somatosensory (ss) one, whereas they are fewer and more scattered in the motor cortex (mo, C). D, E: details of supragranular layers (II-III, D) and deep layers (IV-V, E), showing the prevalence of numerous bipolar ChAT+ neurons in the former (D) and only a few immunopositive cells, both bipolar (arrows) and multipolar (arrowheads), in the latter (E). Scale bar: 112 μm in A; 135 μm in B and C; 87 μm in D and E.