Figure 4

Generation analysis of CD4⁺ cells in mixed splenocyte/lymphocyte cultures isolated from MOG-primed wt and mmp7^-/- mice, using CFSE. (a, b) CD4⁺ cells from wt mice had a small response to IL-2, (c) and a more robust response to MOG (10 μg/ml). For a-c, percentages indicate the proportion of initial cells that divided beyond the 4^th generation, which was the farthest significant generation for media-only controls shown in a. (d, e) CD4⁺ proliferation in response to IL-2 in mmp7^-/- mice was comparable to that in wt cells. (f) MOG-induced proliferation was comparable to that from IL-2 stimulation – less than the responses of wt cells to MOG.