Neural differentiation potential of human bone marrow-derived mesenchymal stromal cells: misleading marker gene expression

Table 2 Primer sequences and PCR conditions

Primer	Forward (5'- 3')	Reverse (5'- 3')	Hybridization temperature (°C)	Cycles	Amplicon size (bp)
ASCL1	aag caa gtc aag cga cag cg	agtcgt tgg agt agt tgg gg	62	35	352
Bag1	tca ccc aca gca at gaga ag	cag aaa acc ctg ctg gat tc	66	30	344
DRD2	tca tcg ctg tca tcg tct tcg	gat gga gat cat gac ggt gac	65	40	344
Engrailed-1	agc cac agg cat caa gaa cg	cac ctg tcc gag tct ttc tc	65	40	303
Enolase2	ggc aaa ggt gtc ctg aa gg c	gtg ccg gcc ttc aac gtg at	67	30	284
GAPDH	tga agg tcg gag tca acg gat ttg gt	cat gtg ggc cat gag gtc cac cac	62	30	983
GFAP	gtg gta ccg ctc caa gtt tgc ag	aat ggt gat ccg gtt ctc ctc	59	40	373
MAP1B	act gca gga cca gga act ac	cag tgt cac ctg cat gtt gc	67	25	255
MAPT	agc tct ggt gaa cct cca aaa tc	cat cca tca taa acc agg agg tg	58	40	361, 454
MBP	cct ggc cac agc aag tac	ggg agc cgt agt gag cag t	61	35	251
Nestin	gcg ttg gaa cag agg ttg gag	gca cag gtg tct caa ggg tag	65	30	385
NEUROD6	ctg aga atc ggc aag aga cc	ctg cac agt aat gca tgc cg	62	35	433
NFH	tga aca cag acg cta tgc gct cag	cac ctt tat gtg agt gga cac aga g	58	35	398
NFL	acc aac gag aag caa gcg ctc	cat cag cgc tat gca gga cac	59	35	590
NFM	aaa gac atc gag gag gcg tc	cgc tgc gta cag aaa act cc	61	35	592
Nurr1	aag gct tct tta agc gca cag	cga tta gca tac agg tcc aac	55	25	518
S100β	gga gac aag cac aag ctg aag	agc tac aac acg gct gga aag	63	30	322
STX1A	atc gca gag aac gtg gag gag	agc gtg gag tgc tgt gtc ttc	67	30	230
SYP	ggt gct gca atg ggt ctt cgc	aag ccg aac acc acc gag gtg	59	35	537
TH	atc cac cat cta gag acc cg	tcc ccg ttc tgc tta cac ag	63	40	824

ISSN: 1471-2202