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Table 2 Primer sequences and PCR conditions

From: Neural differentiation potential of human bone marrow-derived mesenchymal stromal cells: misleading marker gene expression

Primer Forward (5'- 3') Reverse (5'- 3') Hybridization temperature (°C) Cycles Amplicon size (bp)
ASCL1 aag caa gtc aag cga cag cg agtcgt tgg agt agt tgg gg 62 35 352
Bag1 tca ccc aca gca at gaga ag cag aaa acc ctg ctg gat tc 66 30 344
DRD2 tca tcg ctg tca tcg tct tcg gat gga gat cat gac ggt gac 65 40 344
Engrailed-1 agc cac agg cat caa gaa cg cac ctg tcc gag tct ttc tc 65 40 303
Enolase2 ggc aaa ggt gtc ctg aa gg c gtg ccg gcc ttc aac gtg at 67 30 284
GAPDH tga agg tcg gag tca acg gat ttg gt cat gtg ggc cat gag gtc cac cac 62 30 983
GFAP gtg gta ccg ctc caa gtt tgc ag aat ggt gat ccg gtt ctc ctc 59 40 373
MAP1B act gca gga cca gga act ac cag tgt cac ctg cat gtt gc 67 25 255
MAPT agc tct ggt gaa cct cca aaa tc cat cca tca taa acc agg agg tg 58 40 361, 454
MBP cct ggc cac agc aag tac ggg agc cgt agt gag cag t 61 35 251
Nestin gcg ttg gaa cag agg ttg gag gca cag gtg tct caa ggg tag 65 30 385
NEUROD6 ctg aga atc ggc aag aga cc ctg cac agt aat gca tgc cg 62 35 433
NFH tga aca cag acg cta tgc gct cag cac ctt tat gtg agt gga cac aga g 58 35 398
NFL acc aac gag aag caa gcg ctc cat cag cgc tat gca gga cac 59 35 590
NFM aaa gac atc gag gag gcg tc cgc tgc gta cag aaa act cc 61 35 592
Nurr1 aag gct tct tta agc gca cag cga tta gca tac agg tcc aac 55 25 518
S100β gga gac aag cac aag ctg aag agc tac aac acg gct gga aag 63 30 322
STX1A atc gca gag aac gtg gag gag agc gtg gag tgc tgt gtc ttc 67 30 230
SYP ggt gct gca atg ggt ctt cgc aag ccg aac acc acc gag gtg 59 35 537
TH atc cac cat cta gag acc cg tcc ccg ttc tgc tta cac ag 63 40 824