Western blot analysis shows the decrease levels of p-CREB following ID and Hypothyroidism (n = 3 for each group). The levels of p-CREB in ID and hypothyroidism groups were significantly lower than controls on PN21 (A), PN28 (B) and PN42 (C). (a) Representative blots of p-CREB in hipocampal CA1, CA3 and DG regions of control, 5 ppm PTU-treatment, 15 ppm PTU-treatment and iodine-deficient groups. (b) p-CREB levels. Each bar represents the mean ± SEM. *P < 0.05, as compared to control.#P < 0.05, as compared to 5 ppm PTU-treatment group. ΔP < 0.05, as compared to 15 ppm PTU-treatment group.