Western blot analysis shows the decrease levels of t-CREB following ID and Hypothyroidism (n = 3 for each group). The levels of t-CREB in ID and hypothyroidism groups were significantly lower than controls on PN14 (A), PN21 (B), PN28 (C) and PN42 (D). (a) Representative blots of t- CREB in hipocampal CA1, CA3 and DG regions of control, 5 ppm PTU-treatment, 15 ppm PTU-treatment and iodine-deficient groups. (b) t- CREB levels. Each bar represents the mean ± SEM. *P < 0.05, as compared to control. #P < 0.05, as compared to 5 ppm PTU-treatment group. ΔP < 0.05, as compared to 15 ppm PTU-treatment group.