Figure 1

Expression of Wld^S protein and mRNA in non-neuronal tissue. A - Representative examples of bands obtained with quantitative fluorescent western blots from wild-type and Wld^Smouse liver, spleen and cerebellum probed with antibodies against Wld^S protein (Wld-18) and beta-actin (loading control). B - Wld^S protein levels in organs from Wld^Smice expressed as fold change in protein relative to wild-type tissue. Note that all organs except for the liver showed significant levels of Wld^S protein, but none to the same magnitude as found in the cerebellum. C - Wld^SRNA levels in organs from Wld^Smice shown as relative expression normalised to wild-type. Note that RNA levels did not always match protein expression levels (c.f. Panel A). A minimum of 3 mice per genotype were used for all experiments.