Src kinase sustainedly activated in the DG field of hippocampus after ischemia, while its inhibitor SU6656 blocked cell proliferation induced by ischemia. SU6656 was intracerebroventricularly (i.c.v) administratered 20 min before ischemia. (A) Western blot for Src kinase detected by anti-p-Src antibody (Tyr527). (B) DAB staining for Brdu-positive cells in the SGZ of dentate gyrus. (a) Sham rats (n = 4), (b) Rats with 7 days of reperfusion postischemia (n = 5), (c) SU6656-treated ischemic rats (n = 5), and (d) vehicle-treated ischemic rats (n = 4). *P < 0.05 vs. sham group; #P < 0.05 or aP < 0.05 vs. the respective reperfusion group. Bar: 200 μm.