Giant "dark" neurons in the pontine reticular formation (A and B) at 3 hours survival time after intramuscular injection of kainic acid (KA). The Golgi-like staining highlights the giant "dark" neurons of the oral pontine reticular field (PnO; A) and caudal pontine reticular field (PnC; B). The representative EEG period demonstrates the generalized convulsive status epilepticus (C). On the one-hour PSD graphs, there is a smaller peak at 2 Hz after the 4-AP injection a day before the kainic acid treatment (red line), while there is a prominent, high peak in the PSD at 2-2.5 Hz at 3 hours after the KA (D). The control PSD (gray line) was calculated from a 30 min EEG recording before the kainic acid treatment. Abbreviations: ISS and CSS: ipsi- and contralateral somatosensory cortex, CFC: contralateral frontal cortex. Scale bars: A = 100 μm, B = 50 μm, C = 300 μV.