Figure 7From: Progranulin is expressed within motor neurons and promotes neuronal cell survivalpEGFP-N1-hPGRN cloning construct. The human PGRN cDNA (hpgrn) was cloned into the pEGFP-N1 plasmid using EcoR1 and Sal1 restriction enzyme digestion sites. The eGFP molecule is fused to the C-terminus of the PGRN protein, and therefore does not affect its N-terminal signal sequence that is required for entry into the endoplasmic reticulum. Individual 12 cysteine granulin modules are designated 1 through to 7 and A through to G. P is paragranulin, a half-granulin module bearing six cysteine residues.Back to article page