Figure 14From: Progranulin is expressed within motor neurons and promotes neuronal cell survivalPGRN knockdown reduces cell proliferation and exogenous PGRN rescues cell proliferation. (A) AlamarBlue cell proliferation assay allowing the quantitative measurement of cell proliferation from day 0 to day7 in the presence of serum in 96 well plates. PGRN knockdown significantly reduced the cell proliferation rate from day 2-3 to day 7-8 (10 replicates per determination, p > 0.001). (B) Addition of exogenous PGRN rescued NSC-34 cell proliferation from day 2-3 to 4-5 (representative experiment, 10 replicated per determination, P < 0.05). (C) Apoptosis assay based on TUNEL labelling method following 3 days in the presence of serum. PGRN silencing had no significant effect on apoptosis (N = 2, 6 fields 10× magnification per determination).Back to article page