Figure 1

PGRN is expressed by neurons of both central and peripheral nervous systems, in vivo and in vitro in the mouse. Gene expression pattern of murine PGRN in brain (B, C); cervical spinal cord (A, D, E) and primary cultures of dissociated spinal cord-DRG (F, G). In situ hybridization, to detect PGRN mRNA in saggital section of pontine grey matter (A) and cross-section of cervical spinal cord (D, E). The majority of neurons throughout the grey matter of the spinal cord express PGRN as well as ependymal cells and possibly microglial cells (A). Note in particular the robust expression of PGRN mRNA in large motor neurons in panels B, D. Panels C and E illustrate the hybridization signal observed with the sense control applied to serial sections to those shown in panels B and D, respectively. (F) Motor neurons (asterisk) as well as other neuronal subtypes in dissociated spinal cord-DRG cultures, express PGRN; (G) equivalent sense control. Scale bar (panels B-G) represents 20 μm. Original magnification of panel A was 10×