Effects of Chase ABC treatment on neurite outgrowth of neurons derived from NPCs. (A) NPCs were differentiated in 1% FCS for 5 days in the presence or absence of Chase ABC to allow differentiation into neurons with concomitant extension of neurites recognized by β-III tubulin immunostaining. Nuclei were counterstained with Hoechst 33342. Scale bar: 50 μm. (B) Total, average and the longest neurite lengths of each NPC-derived neuron were determined by Neurolucida software. The total neurite length per cell in Chase ABC treated group was longer than that in control, but no differences were found in average neurite length and the longest neurite length between the two groups. (C) The NPC-derived neurons in Chase ABC treated group showed more elaborate networks of neurites, including the number of primary neurite and the branch order, than the control. Data in B and C were measured with Neurolucida system and expressed as the mean ± SEM of four separate experiments with a total of 50 neurons examined in each group. **P < 0.01, treated vs control.