Figure 4From: Chondroitin sulfate proteoglycans regulate the growth, differentiation and migration of multipotent neural precursor cells through the integrin signaling pathwayMigration of NPCs out of neurospheres was enhanced by treatment of Chase ABC. (A) Neurospheres were imaged at 2, 4.5 and 8 hours after plating into the culture wells with or without Chase ABC. (B) The quantification of NPC migration at 2, 4.5 and 8 hours were performed by measuring the diameters of the spheres surrounded by rim of migrating cells, and the results were showed in a time course graph. Data represent the mean ± SEM obtained from three independent experiments with a total of more than 30 neurospheres examined in each time point. * P < 0.05, ** P < 0.01. Scale bars: 200 μm.Back to article page