Migration of NPCs out of neurospheres was enhanced by treatment of Chase ABC. (A) Neurospheres were imaged at 2, 4.5 and 8 hours after plating into the culture wells with or without Chase ABC. (B) The quantification of NPC migration at 2, 4.5 and 8 hours were performed by measuring the diameters of the spheres surrounded by rim of migrating cells, and the results were showed in a time course graph. Data represent the mean ± SEM obtained from three independent experiments with a total of more than 30 neurospheres examined in each time point. * P < 0.05, ** P < 0.01. Scale bars: 200 μm.