Effects of Chase ABC treatment on the proliferation of NPCs in vitro. The dissociated NPCs were cultured in growth medium containing EGF and bFGF for 24 or 48 hours with or without Chase ABC. (A) Bar graph shows that Chase ABC treatment for 24 hours enhanced NPC proliferation at a dose of 0.05 to 5 mU/mL, measured as percentage of control radioactive counts (cpm value). Data represent mean ± SEM of three independent experiments performed in triplicate. * P < 0.05. (B-C) Cell cycle was analysed by FACS, 48 hours after Chase ABC treatment on NPCs. (B) Histograms indicate the DNA distribution in control and Chase ABC treated groups. (C) The percentages of cell populations in G1-, G2-, and S-phases were derived from DNA histograms (B) by a deconvolution analysis. The results showed that the treatment of Chase ABC barely affected the cell cycle except for the G2-phase. Data shown were mean ± SEM of four independent experiments. * P < 0.05.