Figure 4

Organotypic hippocampal culture subjected to OGD. (a) Slices subjected to oxygen-glucose deprived (OGD) condition in the presence of nPLA (0.037 μM-0.15 μM) and propidium iodide (5 μg/ml). Confocal microscopy images show that brighter areas correspond to a high propidium iodide [24] uptake level indicating a higher density of dead or damaged cells. (b) Percentage of cell survival, calculated based on PI intensity on each CA1 and CA3 regions. Each point represents the mean ± SEM (n = 8). #; p < 0.05 and *; p < 0.01. (c) Quantitation of gene expression using real-time PCR. Statistical analysis was performed using one-way analyses of variance (ANOVA; *; p ≤ 0.005.) and a t-test to determine significance between groups for LDH assay (n = 6) and real-time PCR (n = 9).