Anti-apoptotic effect of nPLA (a) Histological and TUNEL analysis of brain sections. High power (×120) photomicrographs of haematoxylin and eosin-stained (b) TUNEL-stained striatal and cortical tissue. Nuclei are indicated by arrows. Rats subjected to transient MCAo were injected intravenously with either vehicle or 0.15 μg/g body weight nPLA. Sham-operated animals underwent the entire surgical procedure other than suture insertion. (c) Quantitative gene expression (real-time PCR) analysis. Gene expression analysis on selected genes for sham operated (Sham), MCAo and MCAo+nPLA brain samples. All measurements were performed in triplicate. Values are expressed as fold change ± SEM, * p < 0.05; ** p < 0.01 by unpaired Student's t-test.