Histological analysis of brain sections. (a) TTC stained coronal brain sections (2 mm thick) of rats treated with 0.15 μg/g body weight nPLA intravenously at 0 min, 5 min and 15 min post-occlusion (n = 6). Surviving cells stained red whilst dead cells remained white. (b) Infarct volumes are expressed as a percentage of the vehicle control ± SEM. *, p < 0.05; **, p < 0.01 using single-factor ANOVA followed by Dunnett multiple comparison test.