Figure 4

LCM of neurons from the pyramidal cell layers of CA1 and CA3 of hippocampus, CbG cells of cerebellum, and layers IV-VI of cerebral cortex, and condition tree and Principal Components Analysis of microarray data from the captured neurons. (A) LCM capture of neurons from the four brain regions. The upper row shows the lightly stained populations of neurons in the respective brain sections, the middle row shows the image of the sections after neuronal capture by LCM, and the bottom row shows the captured neurons on the LCM cap. (B) Condition tree analysis of microarray data. Condition tree analysis was based on expression values of all probesets on the Affymetrix RAE230A GeneChip (color bar shown at right indicates relative gene expression levels). Pearson correlation was used as measurement of similarity. (C) PCA of microarray data. This analysis allowed for the visualization of the relationships among samples from the four brain regions.