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Figure 5 | BMC Neuroscience

Figure 5

From: Mitochondrial BNIP3 upregulation precedes endonuclease G translocation in hippocampal neuronal death following oxygen-glucose deprivation

Figure 5

BNIP3 initiates the nuclear translocation of EndoG following reoxygenation. (A) Representative western blot showing the expression of mitochondrial BNIP3. (B) Quantification of the expression of mitochondrial BNIP3. Data were presented as fold of the control group. *p < 0.05 vs. control group, n = 3. (C) EndoG (red) is located outside the DAPI-stained nucleus (blue) in EGFP-C3 transfected hippocampal neurons (left panel). EndoG translocated to the DAPI-stained nucleus in EGFP-C3-rBNIP3 transfected hippocampal neurons (right panel). (D) Percentage of neurons displaying nuclear EndoG in EGFP-C3 and EGFP-C3-rBNIP3 transfected hippocampal neurons, respectively. Data was presented as the percentage of the transfected cells. *p < 0.05 vs. control group, n = 21-26. (E) EndoG translocated to the nucleus in neg-miRNA transfected hippocampal neurons (left panel) at 6 h after reoxygenation. EndoG located outside of the nucleus in BNIP3-miRNA transfected neurons (right panel) at 6 h after reoxygenation. Arrowhead showed that the EndoG still translocated to the nucleus in BNIP3-miRNA untransfected neurons (right panel) at 6 h after reoxygenation. (F) Percentage of neurons displaying nuclear EndoG in neg-miRNA and BNIP3-miRNA transfected hippocampal neurons, respectively. Data are presented as the percentage of the positive transfected cells. *p < 0.05 vs. control group, n = 40-50.

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