Localization of PMCA isoforms in parasagittal sections of developing mouse cerebral cortex. Images show the V layer of the cortex. PMCA1-4 isoforms were detected in developing pyramidal neurons (arrowheads) from the earliest stages to mature cells. PMCA2 was the highest isoform expressed and the PMCA3 the weakest in all analyzed stages. From P8, the four isoforms were located in the neuropil (asterisk) and in the plasma membrane of the bodies of cortical cells (arrowhead). Cytoplasm of the soma and dendritic trunks were immunonegatives. Scale bar: 50 μm.