Immunodetection of PMCA isoforms in MV from different brain regions. A. MV (20 μg) from cortex, hippocampus and cerebellum at the indicated developmental stages were submitted to electrophoresis, blotted on PVDF membranes and incubated with specific anti-PMCA1, anti-PMCA2, anti-PMCA3 and anti-PMCA4 antibodies. Representative anti-β tubulin immunoreaction as loading control is also shown. B. Quantification of PMCA isoforms (combining all splice variants within each isoform) respect to β tubulin is represented. C. Total PMCA intensity in brain areas obtained from Fig. 2B by adding the band intensities of all isoforms in each stage of development.