Immunohistochemical analysis of TH+ DA neurons in the DMC to show the effects of various drug treatments. Frontal paraffin sections of adult fly brain were stained with anti-TH antibody. Representative sections containing DA neurons in the DMC were shown for each genotype. 25-day-old female flies were analyzed. A-F: Ddc-Gal4>DJ-1A RNAi flies. A: yeast fed no-drug control, B: 20 μg/ml celastrol-treated, C: 50 mg/ml minocycline-treated, D: 5 μg/ml celastrol + 25 mg/ml minocycline-treated, E: 30 mg/ml NBQX-treated, F: 100 mg/ml coQ10-treated. G, H: Ddc-Gal4/+ flies. G: yeast fed no-drug control, H: 30 mg/ml NBQX-treated.