HPLC analysis of the effects of celastrol and minocycline treatments on brain dopamine content. Head homogenates of 10-day-old Ddc-Gal4>DJ-1A RNAi flies treated with celastrol or minocycline were measured for dopamine content by HPLC. These were compared with untreated Ddc-Gal4>DJ-1A RNAi flies and control Ddc-Gal4/+ flies with or without drug treatments. Results are the mean ± SD of 21 homogenized heads for each genotype and drug treatment. *: P < 0.05 and **: P < 0.01 in ANOVA with Dunnett's post test.