Whole-mount confocal immunofluorescence analysis of TH+ neurons in the DMC of Ddc-Gal4>DJ-1A RNAi flies. Whole-mount brain samples of 25-day old Ddc-Gal4>UAS-DJ-1A RNAi (A) or control Ddc-Gal4>UAS-GFP (B) flies were immunostained with anti-TH antibody and TH-positive immunofluorescence signals were detected by confocal microscopy. Representative confocal images of the TH+ neurons in the DMC are shown. Asterisks mark the identifiable TH+ neurons. Note that not only was there a reduction of TH+ neurons in Ddc-Gal4>UAS-DJ-1A RNAi flies compared to the control, the intensity of TH immunoreactivity was also reduced in the remaining neurons.