Figure 2
From: Labelling and targeted ablation of specific bipolar cell types in the zebrafish retina
Retinal expression of eGFP in enhancer trap lines at early larval stages. Fluorescence microscopy of eGFP expression in the Tg(Gal4-VP16;UAS:eGFP)xfz 3line (A-D). A ventral view (A) shows initial eGFP labelling of single cells (arrows) within the ventral-nasal region of the retina at 64 hpf. Lateral views (B-D), with anterior to the left, show increasing numbers of eGFP expressing retinal cells at 68 hpf (B), 3 dpf (C) and 5 dpf (D). (E-H) Expression of eGFP in the Tg(Gal4-VP16;UAS:eGFP)xfz 43line. Ventral view (E) shows strong eGFP labelling in the olfactory placodes (arrowheads) and low levels of expression in single cells within the ventral-nasal region of the retina at 64 hpf (arrows). Lateral views (F-H), with anterior to the left, show increasing numbers of eGFP expressing retinal cells at 68 hpf (F), 3 dpf (G) and 5 dpf (H). Scale bar: 50 μm.