Genistein protects against Aβ25–35 induced apoptosis of PC12 cells through JNK signaling and modulation of Bcl-2 family messengers
© The Author(s) 2017
Received: 11 July 2016
Accepted: 27 December 2016
Published: 12 January 2017
Deposition of aggregated amyloid beta (Aβ) protein is hallmark of Alzheimer’s disease, leading to dysfunction and apoptosis of neurons. The isoflavone phytoestrogen compound genistein (Gen) exerts a significant protective effect against Aβ25–35 induced neurotoxicity and mitochondrial damage in rat pheochromocytoma (PC12) cells. However, the mechanisms underlying Gen’s rescue remain elusive. Therefore we endeavored to research further the molecular mechanisms underlying Gen’s inhibition of Aβ25–35 induced apoptosis of neurons.
We found that Gen dramatically suppressed the activation by Aβ25–35 of p-c-Jun N-terminal kinase (p-JNK), and also inhibited the JNK-dependent decreased of Bcl-w and increased of Bim. Furthermore, Gen significantly reduced the cytoplasmic concentrations of cytochrome c and Smac protein as well as caspase-3 activity. Additionally, pretreatment with JNK inhibitor SP600125 effectively suppressed Aβ25–35 induced PC12 cell cytotoxicity.
Taken together, the results suggested that Gen protects PC12 cells from Aβ25–35 induced neurotoxicity by interfering with p-JNK activation, thus attenuating the JNK-dependent apoptosis through the mitochondrial pathway. These findings constitute novel insights into the pathway for Aβ25–35 toxicity, and the neuroprotective action of Gen.
KeywordsAlzheimer’s disease Aβ25–35 Apoptosis Genistein JNK Bcl-2
The amyloid β protein is a β-sheet peptide compose by 39–43 amino acid residues; its fragment Aβ25–35 (GSNKGAIIGLM), the smallest fragment formed by brain proteases, retains the toxicity of the parent peptide as well as a capacity to form aggregates in vivo. As such, Aβ25–35 promotes to the development of Alzheimer’s disease (AD) pathology and the resultant clinical symptoms [1, 2]. Deposition of Aβ25–35 in brain triggers tau protein phosphorylation and formation of intracellular neurofibrillary tangles (NFT), subsequently leading to mitochondrial dysfunction and membrane rupture, which then proceeds to necrosis or apoptosis . As such, by investigating the pathway by which Aβ25–35 toxicity leads to neuronal apoptosis we may understand the development of new treatment strategies in AD.
Extensive research has shown that Aβ25–35 accumulation is linked with many signaling pathways implicated in neurodegenerative disease. In particular, c-Jun N-terminal kinase (JNK) is a factor in Aβ induced apoptosis of neurons . JNK regulates many transcription factors, including the Bcl-2 family , which importantly controls of the mitochondria apoptosis pathway . Finally, the release of the mitochondrial proteins cytochrome c and second mitochondrion-derived activator of caspase (Smac) into cytoplasm is an important indicator of cellular apoptosis .
Genistein (Gen) is an isoflavone phytoestrogen derived from soybeans. It is present in tofu (soybean curd) and its consumption has shown promising results as a moderator of cognitive deficits in AD . Indeed, substantial evidence suggests that Gen inhibits Aβ25–35-induced toxicity via regulation of many relevant signaling pathways [9–11] and by facilitation of Aβ clearance from the nervous system . Conversely, another study showed that dietary Gen can lead to cognitive impairment . Resolving these contradictory findings requires a better understanding of the molecular mechanisms whereby Gen modulates AD neuropathology. However, differentiated neuron-like rat pheochromocytoma (PC12) cells often used for studying neuroprotection [14, 15].
Given this background, we endeavored to use PC12 cells in order to test whether Gen protects PC12 cells from Aβ25–35 induced neurotoxicity. In particular, we researched the regulatory effects of Gen on the expression of Bcl-2 family members (such as the Bcl-w and Bim) in PC12 cells challenged with Aβ25–35 in vitro. Furthermore, we also examined the effects of Gen on the JNK phosphorylation level upstream in the mitochondrial apoptotic pathway. Our studies in PC12 cells address fundamental aspects of the potential of Gen to ameliorate Aβ-induced neuronal cytotoxicity and apoptotic signaling.
PC12 cells were obtained from the Institute of Biochemistry and Cell Biology (Shanghai, China) cell bank and cultured in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% v/v fetal bovine serum (FBS), 100 U/mL penicillin, and 0.1 mg/mL streptomycin (all from Hyclone, Logan, UT) at 37 °C in a humidified atmosphere of CO2 (5%). After every second day, replaced with fresh media, to promote growth until the cells reach a confluence of 70–80%.
Determination of cell viability
MTT was used to detect viability of PC12 cells. In brief, the cells were cultured in medium at a density 5 × 104 cells per well for 24 h in 96-well plates and then pretreated with or without Gen at final concentrations of 12.5, 25, 50, and 100 μM for 2 h and incubated with Aβ25–35 (20 μM) for another 24 h. The Aβ25–35 (Sigma-Aldrich) was first dissolved in tri-distilled water at a concentration of 1 mM, and then aged for 3 days in a humidified chamber at 37 °C before being added to the culture medium to the final desired concentration. Cells were added to 10 μL of MTT solution medium (5 mg/mL) and then incubated at 37 °C for 4 h. The medium was then carefully removed, and added 150 μL per well DMSO to dissolve the formazan crystals formed in situ. Cell viability was then determined by measuring the absorbance of each well at 570 nm using a microtiter plate reader (Biotek, VT). Each concentration was repeated three times with five replicates per experiment.
Hoechst 33342 staining to detect cell apoptosis
Hoechst 33342 (Beyotime Biotechnology, Haimen, China) was used to identify the apoptotic cells. PC12 cells were cultured and then pretreated with Gen at concentrations of 12.5, 25, 50, and 100 μM for 2 h and incubated with Aβ25–35 (20 μM) for another 24 h. Cells were washed with PBS and then stained with Hoechst 33342 DNA-binding dye (10 mg/L) for 15 min at 37 °C in darkness. Finally, the cells were washed with PBS and examined under a fluorescence microscope (Leica, Germany).
Fluorescence-activated cell sorting (FACS) analysis
PC12 cells were cultured in 6-well plates; treated with 12.5, 25, 50, and 100 μM of Gen for 2 h; and finally incubated with Aβ25–35 (20 μM) for another 24 h. Cell apoptosis was measured using the Annexin V-FITC Apoptosis Detection Kit I (Beyotime Biotechnology, Haimen, China). In brief, the cells were washed once with PBS and digested with trypsin. The subsequently collected cells were washed once with PBS. Next, 5 μL of Annexin V-FITC and 10 μL of propidium iodide (PI) were then added according to the manufacturer’s instructions. And mixing and incubation for 10 min in darkness at room temperature, cells were detected using a FACS (BD Biosciences, San Jose, CA).
RNA extraction and real-time RT-PCR quantitation
PC12 cells were treated with Gen at concentrations of 25 μM for 2 h and with the JNK phosphorylation inhibitor SP600125 (Beyotime Biotechnology, Haimen, China) at a concentration of 100 nM for 1 h; finally incubated with Aβ25–35 (20 μM) for another 24 h. Total RNA was then isolated from the PC12 cells with Trizol reagent (Invitrogen, Carlsbad, CA). RNA concentration and purity were determined using a fluorospectrophotometer (RF-5301PC; Shimadzu, Japan), and RNA integrity was verified by 1% agarose gel electrophoresis. The first strand cDNAs were synthesized from 2 μg of total RNA in a 20 μL reaction volume using reverse transcriptase (Takara Biotechnology, Dalian, China).
Next, 2 μL portions of the reverse transcription product was amplified with the SYBR® Premix Ex Taq™ II (Tli RNaseH Plus) (Takara Biotechnology, Dalian, China). The special primers were designed from their GenBank sequences and synthesized by Bio Basic Inc. (Shanghai, China): 5′-CACTTTCTACAATGAGCTGCG-3′, 5′-CTGGATGGCTACGTACATGG-3′ for β-actin; 5′-GAGTTTGAGACCCGCTTCC-3′, 5′-GTCCTCACTGATGCCCAGTT-3′ for Bcl-w; and 5′-CTTACACGAGGAGGGCGTTT-3′, 5′-CAGTGCCTTCTCCAGACCAG-3′ for Bim. The thermal profile reactions were performed in a real-time PCR system (Bio-Rad, Hercules, CA), and the amplified products were quantified by measuring the calculated cycle thresholds (CT) for individual targets and the β-actin reference mRNA. The 2−ΔΔCT method was used for quantification and statistical analysis.
Cytoplasmic proteins were isolated using a Cytoplasm Protein Extraction Kit. Thereafter, a BCA Protein Assay Kit (Beyotime Biotechnology, Haimen, China) was used to determine the protein concentrations. The samples were boiled for 5 min. Next, portions containing 20 μg protein were separated on 12% SDS–polyacrylamide gel and transferred onto PVDF membranes (Millipore, Bedford, MA) at a current of 200 mA for 40 min. After blocking for 2 h in a TBS containing 0.1% Tween 20 (TBST) and 5% w/v skim milk powder at room temperature, these membranes were incubated overnight at 4 °C with primary antibodies against β-actin, cytochrome c, and Smac (1:300, BOSTER, Wuhan, China), and anti-p-JNK (1:2000, Cell Signaling Technology, Danvers, MA), with dilutions in TBST. After washing three times with TBST, the membranes were incubated with horseradish peroxidase-conjugated secondary antibody (1:5000, BOSTER, Wuhan, China) for 1 h at room temperature and again washed three or four times. The bands were developed using an ECL kit following the manufacturer’s instructions, with β-actin serving as a loading control. The X-OMAT BT films (Carestream, Xiamen, China) were scanned and quantitated using Quantity One software.
Caspase activity assay
To evaluate the activity of caspase-3, cell lysates were prepared after their various respective treatments. Assays were performed on 96-well plates by incubating 10 μL portions of cell lysate per sample in 80 μL reaction buffer [0.1% Nonidet P 40, 20 mM Tris-HCl (pH 7.5), 137 mM NAD, and 10% glycerol] containing 10 μL caspase-3 substrate (2 mM, Ac-DEVD-pNA) following the manufacturer’s instructions. Lysates were incubated in this medium at 37 °C for 2 h, and absorbance measured at 405 nm with the microtiter plate reader (Biotek, VT).
Data were expressed as mean ± SD, and all determinations were repeated three times. The data were analyzed by using SPSS v20.0 software (SPSS Inc., Chicago, IL), and p < 0.05 were considered statistically significant.
Effect of Gen on viability of PC12 cells
Hoechst 33342 staining to detect PC12 cells apoptosis
Using FACS to detect PC12 cells apoptosis
Gen reduced Aβ25–35 induced Bcl-w mRNA decreased and Bim increased
Gen attenuated release of cytochrome c and Smac induced by Aβ25–35
Effect of Gen on regulation of Aβ25–35 induced activity of caspase-3 and JNK
Aβ aggregation and formation of intracellular components of senile plaques (SP) and NFT are key steps during the pathological process of neurodegenerative disease. In vitro studies showed that the overexpression and aggregation of Aβ is an initiator of neuronal degeneration , and intracerebral Aβ injection provokes neuronal damage . The active Aβ proteolytic fragment Aβ25–35 retains the capacity to induce neuronal apoptosis, although uncertainty about the relevant signaling pathways has hindered the development of specific targeted treatments; this motivated the present investigation of the interaction between the phytoestrogen Gen and Aβ toxicity.
The process of programmed cell death through activation of distinct signaling pathways, including the mitochondrial apoptotic pathway —a cascade which is involved in Aβ neuronal toxicity . The mitochondrial apoptotic pathway is initiated by members of the Bcl-2 family. Among these, Bcl-w is widely expressed in mammalian tissues, particularly mature brain . Overexpression of Bcl-w in primary culture neurons  conferred protection from Aβ-induced apoptosis, thus suggesting that Bcl-w may be a constitutive inhibitor of apoptosis. In contrast, Bim is a pro-apoptotic member of Bcl-2 family, which is reported to upregulate in a variety of neuronal death paradigms . Thus, silent inhibition of Bim by antisense and genetic knockout approaches can markedly decrease apoptosis of neurons [23, 24]. Later reports have shown that Aβ25–35 downregulates Bcl-w and upregulates Bim , and Gen inhibits Aβ25–35 induced neurotoxicity via PKC signaling pathway and regulation of the CaMKII/CREB pathway [10, 11]. Present results confirm our hypothesis that the neuroprotective agent Gen should normalize pro-apoptotic alterations in Bcl-w and Bim mRNA expression by Aβ25–35.
Cytochrome c—a key constituent of the electron transfer chain in cellular respiration —is normally confined to the inner mitochondrial membrane. When apoptosis signaling causes irreparable mDNA damage, cells hosting these mitochondria show increased release of cytochrome c into their cytosol, suggesting a mechanistic marker for apoptosis [26, 27]. Therefore, we also monitored cytoplasmic levels cytochrome c and Smac in Aβ25–35-stressed PC12 cells. As expected, Aβ25–35 exposure increased cytosol cytochrome c and Smac, whereas pretreatment with Gen rescued PC12 cells from these increases, indicating protection of mitochondria. Moreover, activity of caspase enzymes play a major role in the modulation of apoptosis , and histopathology shows co-localization of hyperphosphorylated tau protein and caspases in the brainstem of AD patients . In the present study we found that pretreated with Gen attenuated the caspase-3 activity induced by Aβ25–35. This finding implies that Gen interferes with Aβ induced apoptosis in PC12 cells through effects on the mitochondrial apoptotic pathway.
JNK activation is closely linked to distinct apoptotic stimuli, whereas silencing of JNK signaling can protect against apoptosis of neurons . In addition, results of studies in vitro and in vivo show that alterations of JNK pathways are associated with pathogenesis and apoptosis of neurons in AD . Importantly, evidence shows that pretreatment with the JNK inhibitor SP600125 prior to Aβ25–35 exposure blocked expression of Bcl-2 family members, including Bcl-w and Bim . This (in conjunction with the present results) implies that Gen may influence Bcl-2 family expression through JNK signaling. Indeed, these results showed that Gen significantly reduced the phosphorylation of JNK, suggesting that amelioration by Gen of Aβ25–35-induced changes in the mitochondrial apoptotic pathway is mediated by JNK activation.
The pharmacological basis of Gen’s effects may be related to its estrogenic profile. Bagheria et al.  found that Gen treatment ameliorated the Aβ induced impairment of short-term spatial memory via an estrogenic pathway in rats. Indeed, several studies have shown that estrogen can promote the regeneration of stressed neurons, and can protect neurons from death . Moreover, clinical studies show that postmenopausal women treated with estrogen replacement therapy had less memory deficits compared to women not receiving estrogen treatment . An epidemiological survey also showed that estrogen replacement therapy was associated with significantly reduced risk of AD in aged women. However, estrogen therapy is a double-edged sword, imparting neuroprotective effects but also increasing the risk for neoplastic transformation in certain non-neuronal cell types ; this trade-off has limited the use of estrogen for protection against dementia in women.
On the other hand, recent studies showed that several phytoestrogens such as Gen, puerarin, and tanshinone have neuronal protective effects and few side effects, thus favoring further investigations into the clinical use of these compounds. Researchers have shown positive effects of Gen in cancer , cognitive dysfunction , and heart disease . Gen can ameliorate Aβ-induced pathology and astrogliosis [38, 39]. Gen has a different tissue-specific agonist–antagonist profile than estrogen since, while it can be neuroprotective, it does not cause cancer in the uterus and other tissues in analogy to tamoxifen, which is an antagonist on some tissues but an agonist in others. This background motivates the present investigation into the neuroprotective attributes of Gen.
Our results are consistent with the hypothesis that Gen can attenuate Aβ25–35 induced PC12 cells apoptotic through inhibition of Aβ25–35 induced JNK activation, JNK-dependent decreased of Bcl-w and increased of Bim, along with attenuation of cytochrome c and Smac release from the mitochondria, and reduced caspase-3 activity. Furthermore, findings upon concomitant treatment with JNK inhibitor SP600125 and Gen showed that additional factors may mediate resistance to Aβ25–35-triggered apoptosis. We conclude that Gen—a major active ingredient of soybean isoflavones—possessing a good safety profile and merits further investigation as a treatment to suppress neuronal apoptosis.
p-c-Jun N-terminal kinase
- Aβ25–35 :
c-Jun N-terminal kinase
second mitochondrion-derived activator of caspase
phosphate buffered saline
fluorescence-activated cell sorting
FLY writing of the manuscript. QL, GFJ and YJZ participated in the design of the study an analysis of the results. JRC participated in the analysis of the data. HY participated in the design of the study, analysis of the data, corrected the manuscript and guidance through the project. All authors read and approved the final manuscript.
This study is supported by the Science and Technology Plan of Social Development Projects of Guangdong Province (2012B031800430).
The authors declare that they have no competing interests.
Availability of data and materials
The data supporting the conclusions of this article are included within the article and its Additional file 1.
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- Hellstrom-Lindahl E, Viitanen M, Marutle A. Comparison of Aβ levels in the brain of familial and sporadic Alzheimer’s disease. Neurochem Int. 2009;55(4):243–52.View ArticlePubMedPubMed CentralGoogle Scholar
- Basha MR, Murali M, Siddiqi HK, Ghosal K, Siddiqi OK, Lashuel HA, Ge YW, Lahiri DK, Zawia NH. Lead (Pb) exposure and its effect on APP proteolysis and Aβ aggregation. FASEB J. 2005;19(14):2083–4.PubMedGoogle Scholar
- Tabaton M, Piccini A. Role of water-soluble amyloid-beta in the pathogenesis of Alzheimer’s disease. Int J Exp Pathol. 2005;86(3):139–45.View ArticlePubMedPubMed CentralGoogle Scholar
- Morishima Y, Gotoh Y, Zieg J, Barrett T, Takano H, Flavell R, Davis RJ, Shirasaki Y, Greenberg ME. Beta-amyloid induces neuronal apoptosis via a mechanism that involves the c-Jun N-terminal kinase pathway and the induction of Fas ligand. J Neurosci. 2001;21(19):7551–60.PubMedGoogle Scholar
- Yao M, Nguyen TV, Pike CJ. Beta-amyloid-induced neuronal apoptosis involves c-Jun N-terminal kinase-dependent downregulation of Bcl-w. J Neurosci. 2005;25(5):1149–58.View ArticlePubMedGoogle Scholar
- Harris MH, Thompson CB. The role of the Bcl-2 family in the regulation of outer mitochondrial membrane permeability. Cell Death Differ. 2000;7(12):1182–91.View ArticlePubMedGoogle Scholar
- Korga A, Korobowicz E, Dudka J. Role of mitochondrial protein Smac/Diablo in regulation of apoptotic pathways. Pol Merkur Lekarski. 2006;20(119):573–6.PubMedGoogle Scholar
- Thorp AA, Sinn N, Buckley JD, Coates AM, Howe PR. Soya isoflavone supplementation enhances spatial working memory in men. Br J Nutr. 2009;102(9):1348–54.View ArticlePubMedGoogle Scholar
- Liao W, Jin G, Zhao M, Yang H. The effect of genistein on the content and activity of alpha- and beta-secretase and protein kinase C in Aβ-injured hippocampal neurons. Basic Clin Pharmacol Toxicol. 2013;112(3):182–5.View ArticlePubMedGoogle Scholar
- Luo S, Lan T, Liao W, Zhao M, Yang H. Genistein inhibits Aβ (25–35) -induced neurotoxicity in PC12 cells via PKC signaling pathway. Neurochem Res. 2012;37(12):2787–94.View ArticlePubMedGoogle Scholar
- Xi YD, Zhang DD, Ding J, Yu HL, Yuan LH, Ma WW, Han J, Xiao R. Genistein inhibits Aβ25–35-induced synaptic toxicity and regulates CaMKII/CREB pathway in SH-SY5Y cells. Cell Mol Neurobiol. 2016;36(7):1151–9.View ArticlePubMedGoogle Scholar
- Bonet-Costa V, Herranz-Perez V, Blanco-Gandia M, Mas-Bargues C, Ingles M, Garcia-Tarraga P, Rodriguez-Aria M, Minarro J, Borras C, Garcia-Verdugo JM, et al. Clearing amyloid-β through PPARγ/ApoE activation by genistein is a treatment of experimental Alzheimer’s disease. J Alzheimers Dis. 2016;51(3):701–11.View ArticlePubMedGoogle Scholar
- Chatterjee G, Roy D, Khemka VK, Chattopadhyay M, Chakrabarti S. Genistein, the isoflavone in soybean, causes amyloid beta peptide accumulation in human neuroblastoma cell line: implications in Alzheimer’s disease. Aging Dis. 2015;6(6):456–65.View ArticlePubMedPubMed CentralGoogle Scholar
- Ogura Y, Sato K, Kawashima K, Kobayashi N, Imura S, Fujino K, Kawaguchi H, Nedachi T. Subtoxic levels of hydrogen peroxide induce brain-derived neurotrophic factor expression to protect PC12 cells. BMC Res Notes. 2014;7:840.View ArticlePubMedPubMed CentralGoogle Scholar
- Tsai YC, Lee YM, Lam KK, Lin JF, Wang JJ, Yen MH, Cheng PY. The role of heat shock protein 70 in the protective effect of YC-1 on β-amyloid-induced toxicity in differentiated PC12 cells. PLoS ONE. 2013;8(7):e69320.View ArticlePubMedPubMed CentralGoogle Scholar
- Horiuchi M, Maezawa I, Itoh A, Wakayama K, Jin LW, Itoh T, Decarli C. Amyloid beta1-42 oligomer inhibits myelin sheet formation in vitro. Neurobiol Aging. 2012;33(3):499–509.View ArticlePubMedGoogle Scholar
- Miguel-Hidalgo JJ, Vecino B, Fernandez-Novoa L, Alvarez A, Cacabelos R. Neuroprotective role of S12024 against neurodegeneration in the rat dentate gyrus. Eur Neuropsychopharmacol. 1998;8(3):203–8.View ArticlePubMedGoogle Scholar
- Papa S, Skulachev VP. Reactive oxygen species, mitochondria, apoptosis and aging. Mol Cell Biochem. 1997;174(1–2):305–19.View ArticlePubMedGoogle Scholar
- Yin KJ, Lee JM, Chen SD, Xu J, Hsu CY. Amyloid-beta induces Smac release via AP-1/Bim activation in cerebral endothelial cells. J Neurosci. 2002;22(22):9764–70.PubMedGoogle Scholar
- Minami M, Jin KL, Li W, Nagayama T, Henshall DC, Simon RP. Bcl-w expression is increased in brain regions affected by focal cerebral ischemia in the rat. Neurosci Lett. 2000;279(3):193–5.View ArticlePubMedGoogle Scholar
- Yao M, Nguyen TV, Pike CJ. Estrogen regulates Bcl-w and Bim expression: role in protection against beta-amyloid peptide-induced neuronal death. J Neurosci. 2007;27(6):1422–33.View ArticlePubMedGoogle Scholar
- Putcha GV, Moulder KL, Golden JP, Bouillet P, Adams JA, Strasser A, Johnson EM. Induction of BIM, a proapoptotic BH3-only BCL-2 family member, is critical for neuronal apoptosis. Neuron. 2001;29(3):615–28.View ArticlePubMedGoogle Scholar
- Whitfield J, Neame SJ, Paquet L, Bernard O, Ham J. Dominant-negative c-Jun promotes neuronal survival by reducing BIM expression and inhibiting mitochondrial cytochrome c release. Neuron. 2001;29(3):629–43.View ArticlePubMedGoogle Scholar
- Becker EB, Howell J, Kodama Y, Barker PA, Bonni A. Characterization of the c-Jun N-terminal kinase-BimEL signaling pathway in neuronal apoptosis. J Neurosci. 2004;24(40):8762–70.View ArticlePubMedGoogle Scholar
- Hsu MF, Raung SL, Tsao LT, Kuo SC, Wang JP. Cellular localization of the inhibitory action of abruquinone A against respiratory burst in rat neutrophils. Br J Pharmacol. 1997;120(5):917–25.View ArticlePubMedPubMed CentralGoogle Scholar
- Liu X, Kim CN, Yang J, Jemmerson R, Wang X. Induction of apoptotic program in cell-free extracts: requirement for dATP and cytochrome c. Cell. 1996;86(1):147–57.View ArticlePubMedGoogle Scholar
- Verhagen AM, Ekert PG, Pakusch M, Silke J, Connolly LM, Reid GE, Moritz RL, Simpson RJ, Vaux DL. Identification of DIABLO, a mammalian protein that promotes apoptosis by binding to and antagonizing IAP proteins. Cell. 2000;102(1):43–53.View ArticlePubMedGoogle Scholar
- Nicholson DW. Caspase structure, proteolytic substrates, and function during apoptotic cell death. Cell Death Differ. 1999;6(11):1028–42.View ArticlePubMedGoogle Scholar
- Wai MS, Liang Y, Shi C, Cho EY, Kung HF, Yew DT. Co-localization of hyperphosphorylated tau and caspases in the brainstem of Alzheimer’s disease patients. Biogerontology. 2009;10(4):457–69.View ArticlePubMedGoogle Scholar
- Bozyczko-Coyne D, O’Kane TM, Wu ZL, Dobrzanski P, Murthy S, Vaught JL, Scott RW. CEP-1347/KT-7515, an inhibitor of SAPK/JNK pathway activation, promotes survival and blocks multiple events associated with Aβ-induced cortical neuron apoptosis. J Neurochem. 2001;77(3):849–63.View ArticlePubMedGoogle Scholar
- Yarza R, Vela S, Solas M, Ramirez MJ. c-Jun N-terminal Kinase (JNK) signaling as a therapeutic target for Alzheimer’s disease. Front Pharmacol. 2016;6:321.View ArticlePubMedPubMed CentralGoogle Scholar
- Bagheri M, Joghataei MT, Mohseni S, Roghani M. Genistein ameliorates learning and memory deficits in amyloid β1-40 rat model of Alzheimer’s disease. Neurobiol Learn Mem. 2011;95(3):270–6.View ArticlePubMedGoogle Scholar
- Wise PM, Dubal DB, Wilson ME, Rau SW, Liu Y. Estrogens: trophic and protective factors in the adult brain. Front Neuroendocrinol. 2001;22(1):33–66.View ArticlePubMedGoogle Scholar
- Shumaker SA, Legault C, Rapp SR, Thal L, Wallace RB, Ockene JK, Hendrix SL, Jones BN, Assaf AR, Jackson RD, et al. Estrogen plus progestin and the incidence of dementia and mild cognitive impairment in postmenopausal women: the Women’s Health Initiative Memory Study: a randomized controlled trial. JAMA. 2003;289(20):2651–62.View ArticlePubMedGoogle Scholar
- Bang OY, Hong HS, Kim DH, Kim H, Boo JH, Huh K, Mook-Jung I. Neuroprotective effect of genistein against beta amyloid-induced neurotoxicity. Neurobiol Dis. 2004;16(1):21–8.View ArticlePubMedGoogle Scholar
- Taku K, Melby MK, Takebayashi J, Mizuno S, Ishimi Y, Omori T, Watanabe S. Effect of soy isoflavone extract supplements on bone mineral density in menopausal women: meta-analysis of randomized controlled trials. Asia Pac J Clin Nutr. 2010;19(1):33–42.PubMedGoogle Scholar
- Sbarouni E, Iliodromitis EK, Zoga A, Theodorakis GN, Kremastinos DT. The effect of the phytoestrogen genistein on myocardial protection and preconditioning in hypercholesterolemia. Cardiovasc Drugs Ther. 2007;21(5):399–400.View ArticlePubMedGoogle Scholar
- Bagheri M, Roghani M, Joghataei MT, Mohseni S. Genistein inhibits aggregation of exogenous amyloid-bata1-40 and alleviates astrogliosis in the hippocampus of rats. Brain Res. 2012;1429:145–54.View ArticlePubMedGoogle Scholar
- Bagheri M, Rezakhani A, Nystrom S, Turkina MV, Roghani M, Hammarstrom P, Mohseni S. Amyloid beta1-40-induced astrogliosis and the effect of genistein treatment in rat: a three-dimensional confocal morphometric and proteomic study. PLoS ONE. 2013;8(10):e76526.View ArticlePubMedPubMed CentralGoogle Scholar