Volume 9 Supplement 1

Seventeenth Annual Computational Neuroscience Meeting: CNS*2008

Open Access

Using Neurofitter to fit a Purkinje cell model to experimental data

BMC Neuroscience20089(Suppl 1):P84

DOI: 10.1186/1471-2202-9-S1-P84

Published: 11 July 2008

The cerebellar Purkinje cell is a highly complex neuron that has different firing behaviors, that contains many different ionic mechanisms and that has a complicated dendritic morphology. Therefore models of this neuron are difficult to hand-tune. We used Neurofitter [1], an automated neuron model parameter search tool, to fit both the passive parameters of a neuron model and the maximal conductances of the ion channels to an experimental data set.

The approach is based on the phase-plane trajectory density method [2] that evaluates the difference between the experimental voltage traces and the model output. Optimization algorithms like Evolution Strategies and Mesh Adaptive Search were used to search the parameter space of the model.

The Neurofitter method was already tested before by fitting the parameters of a Purkinje cell model [3] to output generated by the model itself [4], but now we show results that also use experimental data to fit a new version of the Purkinje cell model with updated kinetics. The traces that were used as goal of the optimization consisted of voltage responses of a Purkinje cell neuron to current steps with different amplitude.



WVG is supported a Research Assistant of FWO-Vlaanderen. Experimental data was provided by Arnd Roth and Michael Häusser, UCL, London, UK.

Authors’ Affiliations

Computational Neuroscience Unit, Okinawa Institute of Science and Technology
Antwerp Theoretical Neurobiology, Universiteit Antwerpen


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© Van Geit and De Schutter; licensee BioMed Central Ltd. 2008

This article is published under license to BioMed Central Ltd.