Recording long-term potentiation of synaptic transmission by three-dimensional multi-electrode arrays
© Kopanitsa et al; licensee BioMed Central Ltd. 2006
Received: 23 May 2006
Accepted: 30 August 2006
Published: 30 August 2006
Multi-electrode arrays (MEAs) have become popular tools for recording spontaneous and evoked electrical activity of excitable tissues. The majority of previous studies of synaptic transmission in brain slices employed MEAs with planar electrodes that had limited ability to detect signals coming from deeper, healthier layers of the slice. To overcome this limitation, we used three-dimensional (3D) MEAs with tip-shaped electrodes to probe plasticity of field excitatory synaptic potentials (fEPSPs) in the CA1 area of hippocampal slices of 129S5/SvEvBrd and C57BL/6J-TyrC-Brd mice.
Using 3D MEAs, we were able to record larger fEPSPs compared to signals measured by planar MEAs. Several stimulation protocols were used to induce long-term potentiation (LTP) of synaptic responses in the CA1 area recorded following excitation of Schäffer collateral/commissural fibres. Either two trains of high frequency tetanic stimulation or three trains of theta-burst stimulation caused a persistent, pathway specific enhancement of fEPSPs that remained significantly elevated for at least 60 min. A third LTP induction protocol that comprised 150 pulses delivered at 5 Hz, evoked moderate LTP if excitation strength was increased to 1.5× of the baseline stimulus. In all cases, we observed a clear spatial plasticity gradient with maximum LTP levels detected in proximal apical dendrites of pyramidal neurones. No significant differences in the manifestation of LTP were observed between 129S5/SvEvBrd and C57BL/6J-TyrC-Brd mice with the three protocols used. All forms of plasticity were sensitive to inhibition of N-methyl-D-aspartate (NMDA) receptors.
Principal features of LTP (magnitude, pathway specificity, NMDA receptor dependence) recorded in the hippocampal slices using MEAs were very similar to those seen in conventional glass electrode experiments. Advantages of using MEAs are the ability to record from different regions of the slice and the ease of conducting several experiments on a multiplexed platform which could be useful for efficient screening of novel transgenic mice.
The dynamically changing strength of connections between neurones was proposed to be a mechanism for memory formation more than a century ago [1–3]. In 1949, Hebb provided a theoretical framework for this hypothesis  and in the 1960s this concept gained crucial experimental support when it was discovered that neurones can alter their firing properties upon experiencing particular patterns of external stimulation, i.e. they exhibit synaptic plasticity . A classical example of synaptic plasticity is long-term potentiation (LTP) discovered in the dentate gyrus more than three decades ago . Traditionally, LTP is defined as a prolonged enhancement of synaptic responses of a neurone or a neuronal ensemble after short periods of high-frequency stimulation . Properties of LTP such as permanence, associativity and input specificity suggested that it could be a physiological basis of certain types of memory [8–11]. The straightforward relationship between LTP and memory has recently been questioned , however in the amygdala and hippocampus, learning has been found to involve increases of the synaptic output [13, 14]. As LTP and cognitive functions often rely on common membrane ion channels and signalling pathways [15–17], in vitro studies of synaptic plasticity are vital for characterisation of mutant animals with potential cognitive disturbances [18, 19]. Mutant mice have become a standard tool for dissection of the molecular basis of LTP especially since pharmacological reagents are available to only a subset of synapse proteins. The Genes to Cognition programme aims to systematically study LTP in 100 mutant mouse lines where components of synaptic complexes are disrupted . With large scale mouse mutagenesis programs now in progress , some with the aim of disrupting all genes in the mouse genome (European Union Conditional Mouse Mutant Program) , it is essential to have rapid and scalable tools for studying LTP in hundreds (or perhaps thousands) of mutant mouse lines.
Another problem is the absence of standard experimental procedures in the LTP field. Most common LTP experiments performed in the synaptic pathway between CA3 and CA1 pyramidal neurones of the hippocampus rely on a multitude of induction protocols (A. Howell, M. Marshall, S. Grant, manuscript in preparation). Moreover, information about the position of stimulating and recording electrodes with respect to each other and in relation to the laminar structure of the hippocampus is lacking in many reports making their cross-comparison difficult.
Such problems can be at least partially overcome when multi-electrode arrays (MEAs) are employed . They have become popular tools for recording spontaneous and evoked activity from excitable tissues [24–28]. For the purposes of LTP analysis, an orderly arrangement of MEA electrodes allows for a precise geometrical assignment of stimulation and recording sites. Current commercial hardware permits the operation of several MEA set-ups by a single computer effectively increasing the output and minimising the number of animals needed for long-term LTP experiments. Several reports have demonstrated the possibility of inducing LTP in hippocampal slices using MEAs [29–34]. However, no systematic study of different induction protocols using MEA technology has yet been published. Furthermore, in all these studies, planar MEAs were employed that could not record activity from the deeper, presumably healthier layers of the slice. In this study, we present our data on the LTP of extracellular field potentials recorded by MEAs with three-dimensional tip-shaped electrodes which allow the recording of larger signals and injection of stronger currents in slice tissue . We show that there is a gradient of the degree of LTP in stratum radiatum (SR) of the CA1 area of hippocampal slices of 129S5/SvEvBrd (129S5) and albino C57BL/6J-TyrC-Brd (C57) mice. These strains were chosen as they are mainstays of large scale mouse knockout programmes . In this work, we test three different LTP-inducing protocols and investigate sensitivity of LTP to the inhibition of N-methyl-D-aspartate (NMDA) receptors.
Properties of field excitatory postsynaptic potentials in CA1 area of the hippocampus recorded by 3D MEAs
Similar results were obtained for I/O relationships in 13 slices from C57 mice (data not shown).
Long-term potentiation of synaptic responses in SR varies with recording location
Monitoring long-term potentiation in two-pathway stimulation experiments
In our study, we sought to assess the applicability of 3D MEAs for studies of LTP in mouse hippocampal slices. Previous attempts to use MEA platforms for recording synaptic activity employed planar electrodes that sample signals mostly from the surface of the slice [29–33, 47]. In contrast, conventional glass or metal electrode recordings are performed usually at some depth inside the slice tissue where neurones are presumed to be in more physiological, non-damaged conditions. Therefore, we were particularly interested in conducting LTP experiments using 3D, tip-shaped arrays that combine benefits of MEA technology with the possibility to record closer to the source of the signal coming from healthier layers of the slice .
Basic features of fEPSPs recorded by means of 3D MEAs were generally in agreement with previous reports on laminar profiles of synaptic responses to excitation of Schäffer collateral/commissural fibres in the CA1 area of the hippocampus in vitro [48–50] and in vivo [51–53]. Large negative extracellular responses were recorded in SR that reflected synaptic activation of apical dendrites of CA1 pyramidal cells (electrodes 2–4 in Figs 1B, 3A). Pre-synaptic fibre volleys were hard to distinguish and usually more clearly observed following stimulation of the subicular side of CA1 in two-pathway experiments (data not shown). At submaximal stimuli (<3.0 V), fEPSP amplitude was larger in the medial and distal parts of SR (Fig. 2A), possibly pointing to the site of termination of the majority of afferent fibres. At higher strengths of stimulation, there was no statistical difference between sizes of fEPSPs recorded in the three locations within SR. The reason for the latter could be generation of dendritic spikes at proximal apical dendrites that led to disproportionably greater increase of fEPSPs in this location [51, 52, 54, 55]. Weak to moderate stimulation of Schäffer collateral/commissural fibres caused positive deflections of the extracellular fields in SP and SO (electrode 5 in Figs 1B, 3A) indicating intracellular spread of synaptic current into the opposing dendritic arborisation . At larger stimuli, a population spike  was observed in many but not all slices (e.g. electrode 5 in Figs 1B; .Additional file 2).
In contrast to many reports that used planar MEAs for fEPSP measurements, application of 3D MEAs permitted routine recording of synaptic potentials of several millivolts in amplitude (Figs. 1B; Additional file 2), which to the best of our knowledge were among the largest ever reported in MEA experiments. Effective excitation of nerve fibres through tip-shaped electrodes of 3D MEAs allowed us to select recording electrodes at a considerable distance from the stimulation site(s) (≥400 μm, Figs. 1A, 5A). Therefore, we were able to evoke fEPSPs that were sufficiently large and at the same time could be reliably potentiated in a pathway-specific manner. In our opinion, precise information about relative positions of recording and stimulating electrodes in a hippocampal slice is important for comparison of results between different laboratories. Having analysed 89 papers from a mouse knock-out plasticity phenotype database (A. Howell, M. Marshall, S. Grant, manuscript in preparation) which studied LTP of fEPSPs in the CA1 area, we were surprised to find that only ten of them mentioned the distance between stimulating and recording electrodes. Relative location of the recording electrode with respect to part of SR (proximal, medial or distal) was stated explicitly only in one of these 89 reports. It has been recognised however that relative dendritic location of electrodes could affect the degree of LTP in the hippocampus [29, 50, 56] and neocortex . Therefore, we sought to investigate whether spatial differences in LTP existed in our preparation.
Using fairly dense 8 × 8 3D MEAs with inter-electrode distance of 100 μm we compared responses from three recording electrodes in the proximal, medial and distal parts of the along apical dendritic area of CA1 pyramidal cells (Fig. 1A). Applying baseline stimulation and subsequently TBS through one of the electrodes situated in the mid-distal part of SR on the border between CA1 and CA3 areas, we demonstrated that the largest potentiation of fEPSPs occurred in proximal dendritic sites within 50–80 μm of SP (Figs. 3, 4). This difference in potentiation could not be accounted for just by the fact that for a given submaximal stimulus, medial fEPSPs were larger that proximal ones (Fig. 2) and therefore less prone to potentiation. As follows from Fig. 4B, the ratio of post- to pre-TBS fEPSP was noticeably bigger in proximal sites. Another explanation for larger LTP at proximal part of SR could be contamination of proximal recording waveforms by population spikes that originated in the cell body. It should be noted that population spikes were not seen consistently in all recordings (for an example, see Additional file 1) and if observed, they were mostly pronounced at high stimulus strengths (Fig. 1B, electrode 5). Fig. 3A demonstrates that larger enhancement of proximal fEPSP (electrode 4) was recorded in the absence of a strong population spike (electrode 5) able to invade apical dendrites. Nonetheless, since amplitudes of population spikes are known to undergo much stronger potentiation compared to fEPSPs (so-called "E-S potentiation") [6, 58–60], the fact that we measured amplitude rather than initial slope of our responses could lead to artificial overestimation of proximal LTP due to significant population spike contribution to compound fEPSP. We did not attempt measuring fEPSP slopes routinely, because our analysis software could calculate slopes only between integer milliseconds making it difficult to assign similar regions of the rising part of fEPSP among different recordings. In order to test if conventional slope calculation would yield consistently smaller estimates of LTP compared to peak amplitude measurements, we calculated slopes within initial one or two milliseconds of fEPSP traces for experiments depicted on Fig. 6A. No significant differences were observed in estimates of LTP when using slope or peak amplitude measurements in either 129S5 (peak: 191 ± 39 %; slope: 220 ± 57 %; t(11)=-1.81, P = 0.097) or C57 mice (peak: 187 ± 44%; slope: 200 ± 69 %; t(13) = -1.03; P = 0.323). Similarly, no large discrepancies between these two measures of LTP have been found in other reports [61, 62].
Greater LTP in proximal dendrites could be a consequence of an altered balance between excitation and inhibition following LTP induction. Simultaneously with LTP of fEPSPs, tetanic stimulation can cause long-term depression of inhibitory signals . On the other hand, since the density of inhibitory synapses is maximal in the proximal part of CA1 apical dendrites , then disinhibition could potentially have a greater effect on fEPSPs in this region.
Larger LTP at proximal as compared to distal SR recording sites was reported in conditions when pressure ejection of tetrodotoxin in SO was used to prevent post-synaptic spiking . There are several methodological differences between the latter study and our experiments: we used mouse whole hippocampal slices and no pharmacological inhibitors, whereas Kolta et al. employed rat mini-slices of CA1 area and blockers of GABAA and GABAB receptors . Nonetheless, collectively these data indicate that somatic population spikes are unlikely to be principal contributors to increased proximal fEPSPs after LTP induction. The cause of differences in LTP expression across SR levels may stem from a greater propensity of proximal dendrites to generate dendritic spikes. Current-source density analyses demonstrated that in response to the orthodromic stimulation CA1 proximal apical dendrites produce compound fEPSPs that contain a tetrodotoxin-sensitive component that precedes the population spike and is spatially distinct from synaptic currents [49, 52, 54]. It has been suggested that in vivo somatic action potentials are, in fact, initiated in proximal SR . Dendritic spikes were also shown to be prerequisites of somatic firing, because even maximal stimulation of Schäffer collaterals failed to fire pyramidal cell bodies if tetrodotoxin was ejected locally onto proximal apical dendrites . Of particular relevance to our data is the observation that induction of LTP leads to preferential enhancement of the proximal dendritic current sink . Taken together these studies demonstrate that voltage-dependent conductances complement apical synaptic currents in shaping fEPSPs and amplify depolarisation and calcium entry during tetanic trains [66–68]. There is evidence about non-uniform distribution of voltage-dependent channels in CA1 apical dendrites , however, it is not quite clear which of them could account for the spatial gradient of LTP observed in this and other studies [29, 56].
Two-pathway stimulation experiments (Fig. 5) have confirmed that increase of proximal fEPSPs, as a result of LTP, was specific to the tetanised pathway and did not reflect a generalised heterosynaptic enhancement of post-synaptic excitability. HFS and TBS markedly augmented fEPSPs that stayed elevated for at least an hour (Figs. 6A, 7A). The degree of LTP was comparable with estimates in other reports that employed similar protocols in mouse hippocampal slices [19, 70–74]. Plasticity induced by HFS and TBS was entirely dependent on NMDA receptors, as incubation with a competitive NMDA receptor blocker completely abolished LTP (Figs, 6B, 7B). This is an agreement with published data on high sensitivity of LTP induced by these protocols to inhibition of NMDA receptors [71, 75, 76].
Brief episodes of TPS are known to evoke LTP which is dependent on activity of protein kinase A and mitogen-activated protein kinase ERK [42–44, 71, 77]. We were particularly interested in this form of plasticity as it strongly correlated with the learning ability of rats in the Morris water maze . Also, impairment of TPS-evoked LTP that paralleled behavioural abnormalities was found in mutant mice lacking some intracellular signalling proteins [19, 78, 79]. Our initial attempts to induce LTP by 150 baseline strength pulses repeated at 5 Hz however were not successful. Most probably, this was because monopolar stimulation through MEA electrodes was not strong and co-operative enough at this frequency to elicit the complex spiking required for induction of LTP . Having elevated stimulus strength to 150% of the baseline during TPS episode, we managed to evoke moderate but persistent potentiation in most of the slices (Fig. 8A; Additional file 2). Some reports mentioned the need for somewhat larger stimulus strengths to generate reliable complex spikes and stable LTP by TPS [79, 80]. The relatively small degree of LTP observed by us might be explained also by the difference in incubation conditions. Most previous accounts of TPS LTP were based on hippocampal slices that were kept in interface chambers [43, 44, 46, 71] which could impart different metabolic properties on brain tissue compared to submerged chambers . Blocking NMDA receptors with 50 μM D,L-AP5 inhibited TPS-induced LTP (Fig. 8B) which agrees with published data [43, 44, 46]. Interestingly however, incubation with D,L-AP5 did not always abolish complex spiking in our slices (data not shown), as reported by others . In the presence of D,L-AP5, LTP of fEPSPs was small but significant 110 ± 9% (t(5) = -3.32; P < 0.05). As overall TPS-induced LTP was quite modest in our experiments, the size of this previously documented NMDA-independent component [43, 44, 46] was minimal.
For our study, we intentionally chose C57 and 129S5 inbred mouse strains that are often used in the creation of genetically modified mice . Several studies have pinpointed differences in synaptic plasticity, neurochemistry and behavioural performance among various inbred strains [83–87]. This variability could complicate comparative phenotyping of transgenic mice obtained on different backgrounds. For example, Nguyen and colleagues reported that TBS and multiple trains of HFS evoke lower levels of LTP in the CA1 area of 129/SvEms mice as compared to C57 strain [84, 85]. In our experimental setting, we observed no difference in any kind of LTP between C57 and 129S5 strains (Figs. 6A, 7A, 8A). This outcome may seem somewhat surprising as 129S5 strain is closely related to 129/SvEms . This discrepancy could be explained by methodological variations, in particular with regard to TBS that comprised twice as many stimuli in our experiments compared to reports [84, 85]. On the other hand, Nguyen et al. detected differences in LTP between 129/SvEms and C57 strains after both one  and four  1 s-long trains of 100 Hz, whereas we saw similar LTP levels in C57 and 129S5 mice after two such trains (Fig. 6A). Thus, it is hard to conclude whether discrepancy between our results and findings of Nguyen et al. are due to methodological differences or variations within 129 sub-strains . Despite our experiments failed to reveal differences in LTP among C57 and 129S5 mice, they do not rule out the notion that inbred mouse strains are heterogeneous with respect to synaptic plasticity [83–87]. Clearly, more data from other strains are necessary to determine the extent of variation of plasticity properties in MEA experimental settings. Another ultimate test of the sensitivity of MEA-based approach to study LTP would be its ability to provide a clear differentiation between wild-type and mutant mice with well established plasticity phenotypes. Therefore, in our future experiments, we shall use several previously characterised knock-out mice to further validate the utility of the MEA platform for large scale plasticity screening. In summary, our study has shown that MEAs could be used to acquire large signals, comparable with fEPSPs recorded by standard extracellular recording techniques. Utilising MEAs, we were able to reliably induce large and persistent LTP of fEPSPs in hippocampal slices by established stimulation protocols. Consequently, MEAs should provide a powerful methodology for plasticity studies with the capacity to simultaneously collect information from several sites. The versatility of MEA-based approach in stimulation of the slice tissue by multiple electrodes makes it especially useful for synaptic tagging and spike-timing dependent plasticity experiments.
Application of 3D MEAs allowed for a detailed examination of the synaptic transmission in acute slices of the mouse hippocampus. Simultaneous recording by several electrodes demonstrated a spatial gradient of LTP in SR of the CA1 area of the hippocampus and highlighted proximal regions of apical dendrites as sites where post-synaptic responses undergo largest plastic changes. LTP evoked by diverse stimulation patterns using MEAs was pathway-specific and dependent on the activity of NMDA receptors. Robust performance of 3D MEAs combined with the multiplexing potential of MEA technology proves that MEAs could be effectively used for phenotypic characterisation of transgenic animals.
Preparation of hippocampal slices
Experiments were performed on hippocampal slices obtained from 2–4 months old 129S5/SvEvBrd and albino C57BL/6J-TyrC-Brd mice bred at the Wellcome Trust Sanger Institute. Animals were killed by cervical dislocation in accordance with Schedule 1 to the U.K. Animals (Scientific Procedures) Act 1986. Whole brain was immediately transferred to a beaker containing ice-cold "cutting" solution of the following composition (in mM): sucrose 110, NaCl 60, NaHCO3 28, NaH2PO4 1.25, KCl 3, MgSO4 7, CaCl2 0.5, glucose 5, sodium ascorbate 0.6, phenol red 0.015. Prior to use, this solution was thoroughly saturated with a gas mixture of 95%O2/5%CO2 to maintain pH level within physiological range (7.25–7.35). Brain was allowed to chill for 2–3 min and then it was trimmed and mounted on the stage of a Vibroslice MA752 (Campden Instruments, Loughborough, UK) with SuperGlue in such a way, so that the blade would cut through hemispheres at an angle of 20–30° their horizontal planes . "Cutting" solution in the temperature-controlled Peltier bath was cooled to 3.5–4.0°C and constantly bubbled with a mixture of 95% O2 and 5% CO2 . Slices were cut at 350 μm by stainless steel blades using minimal speed of blade advancement and vibrating frequency set at "6" mark. Eight to ten slices containing medial segments of the hippocampus with overlaying cortical areas were trimmed of the remaining tissue, placed into a well of a slice chamber (Fine Science Tools, Foster City, CA) and kept submerged under the constant flow (2 ml/min) of fresh artificial cerebrospinal fluid (ACSF) containing (in mM): NaCl 124, NaHCO3 25, NaH2PO4 1, KCl 4.4, MgSO4 1.2, CaCl2 2, glucose 10, phenol red 0.015. When slices were being transferred to the slice chamber, ACSF temperature was maintained at 23°C and then gradually increased to 29–31°C for the rest of the incubation period. Slices rested in these conditions for at least two hours before experiments commenced.
The MEA60 electrophysiological suite was used for fEPSP recordings (Multi Channel Systems, Reutlingen, FRG). Two set-ups consisting of a MEA1060-BC pre-amplifier and a filter amplifier (gain 1100× or 550×) were run in parallel by a data acquisition card governed by MC_Rack 18.104.22.168 software. Raw electrode data were digitised at 10 kHz and recorded on a PC hard disk for further analysis. To record fEPSPs, hippocampal slices were placed into the well of an MEA biochip filled with ACSF. Two types of single-well biochips with 3D, tip-shaped electrodes were used: 1) 8 × 8 3D MEAs that had electrode height of 25–35 μm and electrode spacing of 100 μm; 2) 5 × 13 3D MEAs with electrode height of 35–45 μm and electrode spacing of 140 and 200 μm (Ayanda Biosystems, Lausanne, Switzerland). The bath was grounded via an Ag/AgCl pellet attached to the MEA amplifier ground socket. Slice position and contact with electrodes were secured by a nylon mesh glued to a flattened piece of platinum wire. In some experiments where exact positioning was vital, slices were immobilised by a silver ring with attached nylon mesh that was lowered vertically by a one-dimensional U-1C micromanipulator (You Ltd, Tokyo, Japan). MEA biochips were fitted into the pre-amplifier case and fresh ACSF was delivered to the MEA well through a temperature-controlled perfusion cannula that warmed perfusing media to 32°C. Monopolar stimulation of Schäffer collateral/commissural fibres through array electrodes was performed by STG2008 stimulus generator (Multi Channel Systems, Reutlingen, FRG). Biphasic (positive/negative, 100 μs/a phase) voltage pulses were used. Amplitude, duration and frequency of stimulation were controlled by MC_Stimulus II software. In one-pathway experiments utilising 8 × 8 3D MEAs, one electrode in the medial or distal part of SR near CA1/CA3 border was chosen for stimulation. A column of recording electrodes that was perpendicular to pyramidal cell layer was selected in the CA1 area. The shortest normal between this column and stimulation electrode was 400 or 500 μm. In two-pathway experiments conducted on 5 × 13 3D MEAs, a single principal recording electrode was picked in proximal part of CA1 SR. To stimulate control and test pathways, two stimulation electrodes were assigned on the subicular side and on the CA3 side of SR respectively. The distance from the recording electrode to the test stimulation electrode was 400–560 μm and to the control stimulation electrode 310–560 μm. To evoke orthodromic fEPSPs, stimulation electrodes were activated at a frequency of 0.02 Hz. Maximum possible stimulation of 3.7 and 4.5 V could be applied to electrodes using 8 × 8 and 5 × 13 3D MEAs respectively. Population fEPSPs of 1–4 mV amplitude were recorded in the CA1 area depending on the electrode location. In contrast to most recordings obtained by conventional glass electrodes, initially negative-going fEPSPs recorded by MEAs always had a pronounced positive shoulder throughout SR. We have not investigated the source of this phenomenon in detail, but as the waveform pattern was exactly the opposite in SO, (i.e. initially positive deflection and then a negative wave in response to stimulation of Schäffer collateral/commissural fibres) we reasoned that this secondary potential had a synaptic origin and was not an abnormal stimulation artefact. Following at least 15–30 min of equilibration period inside an MEA well, I/O relationships were obtained and baseline stimulation strength was set to evoke a response that corresponded to 40% of the maximal attainable fEPSP at the recording electrode located in proximal SR. Slices that had maximum responses less than 1 mV in the test pathway were discarded. LTP was induced after 30 min of stable baseline responses by applying patterned trains of stimuli to a single stimulation electrode in one-pathway experiments or to the test electrode in two-pathway trials. Three stimulation paradigms were used: 1) TBS that comprised three trains administered at 20 s intervals with 10 bursts given at 5 Hz per train and 4 pulses given at 100 Hz per burst. When working with 5 × 13 3D MEAs, baseline stimulation intensity was not altered during TBS, however in experiments that used 8 × 8 3D MEAs, it was increased to 1.25× baseline strength; 2) HFS that consisted of two trains administered at a 50 s interval with 100 baseline intensity pulses given at 100 Hz per train; 3) TPS that involved a single train of 150 pulses elicited at 5 Hz using 1.5× baseline stimulus intensity.
Amplitude of the negative part of fEPSPs was used as a measure of the synaptic strength. Differences between groups were detected using mixed or repeated-measures ANOVA as appropriate. Bonferroni/Dunn's post hoc tests were done when the ANOVA F-value indicated significant differences (α = 0.05). LTP plots were normalised to the average of the first five baseline points. Statistical comparisons of normalised fEPSP amplitudes before and after LTP were performed by paired Student's t-test using averages of five data points just before and 60 min after LTP induction. Unpaired Student's t-test was used to examine differences in LTP levels between strains and also between D,L-AP5-treated and untreated slices. Values were considered to be different if P < 0.05. All reported values represented mean ± standard deviation with n indicating number of slices. Statistics were calculated using Statview 5.0.1 for Mac (SAS Institute, Cary, North Carolina) and Origin 7.0 (OriginLab, Northampton, Massachusetts).
129S5/SvEvBrd mouse strain
C57BL/6J-TyrC-Brd mouse strain
field excitatory post-synaptic potential
high frequency stimulation
stratum lacunosum moleculare
We would like to thank Tom O'Dell and Paul Charlesworth for their practical advices and critical reading of the manuscript. We are grateful to Lianne Stanford for her help with the statistical analysis. Expert technical assistance of Nick Best in setting up MEA suite is greatly appreciated. This work was supported by the Wellcome Trust Genes to Cognition Programme.
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