Response of SII cortex to ipsilateral, contralateral and bilateral flutter stimulation in the cat
© Tommerdahl et al; licensee BioMed Central Ltd. 2005
Received: 22 December 2004
Accepted: 14 February 2005
Published: 14 February 2005
A distinctive property of SII is that it is the first cortical stage of the somatosensory projection pathway that integrates information arising from both sides of the body. However, there is very little known about how inputs across the body mid-line are processed within SII.
Optical intrinsic signal imaging was used to evaluate the response of primary somatosensory cortex (SI and SII in the same hemisphere) to 25 Hz sinusoidal vertical skin displacement stimulation ("skin flutter") applied contralaterally, ipsilaterally, and bilaterally to the central pads of the forepaws. A localized increase in absorbance in both SI and SII was evoked by both contralateral and bilateral flutter stimulation. Ipsilateral flutter stimulation evoked a localized increase in absorbance in SII, but not in SI. The SII region that responded with an increase in absorbance to ipsilateral stimulation was posterior to the region in which absorbance increased maximally in response to stimulation of the contralateral central pad. Additionally, in the posterior SII region that responded maximally to ipsilateral stimulation of the central pad, bilateral central pad stimulation approximated a linear summation of the SII responses to independent stimulation of the contralateral and ipsilateral central pads. Conversely, in anterior SII (the region that responded maximally to contralateral stimulation), bilateral stimulation was consistently less than the response evoked from the contralateral central pad.
The results indicate that two regions located at neighboring, but distinctly different A-P levels of the anterior ectosylvian gyrus process input from opposite sides of the body midline in very different ways. The results suggest that the SII cortex, in the cat, can be subdivided into at least two functionally distinct regions and that these functionally distinct regions demonstrate a laterality preference within SII.
There is general agreement that in cats and monkeys (and presumably in humans) the spike discharge activity a mechanical stimulus sets up in rapidly adapting (RA), slowly adapting (SA), and Pacinian (PC) skin mechanoreceptors is projected centrally, at short latency and with relatively minor transformation, to primary somatosensory cortex (both SI and SII) in the contralateral hemisphere. There is no consensus, however, about the way in which the stimulus-evoked response in the ipsilateral hemisphere contributes to cerebral cortical somatosensory information processing and somatosensation. A distinctive property of SII is that it is the first cortical stage of the somatosensory projection pathway that integrates information arising from both sides of the body. Similar to SI, SII possesses a clear topographic organization , but unlike SI, a significant fraction of SII neurons possess bilateral receptive fields (RFs). The fraction of SII neurons with bilateral RFs varies from one topographic region of SII to the next. While ipsilateral input to SII is widely accepted, the role(s) of this input in somatosensory information processing remains uncertain. To investigate the effects on SII input deriving from mechanoreceptors in ipsilateral skin regions, the technique of optical intrinsic signal (OIS) imaging was used to assess the impact on SII of ipsilateral input on the response of SII to a contralateral input. The responses to contralateral, ipsilateral and bilateral vibrotactile stimulation (25 Hz – "flutter") of the forepaw of the cat were quantified and compared to make this assessment. Although evoked responses in both SI and SII were imaged in the studies, the primary focus of this report is the response of SII to the aforementioned stimuli.
The findings of this study demonstrated clearly that the anterior and posterior regions of SII process bilateral inputs very differently. At the locus of the maximal OIS response evoked in the posterior region by an ipsilateral stimulus, bilateral stimulation evoked a response that was, on average, about 25% larger than that evoked from the contralateral stimulus site. Conversely, at the locus of the maximal OIS response evoked by contralateral stimulation in the anterior region, bilateral stimulation evoked a response that was, on average, 35% lower than the activity evoked by a contralateral stimulus. This discrepancy between the optical responses of the anterior and posterior regions could be related to neurophysiological observations reported in earlier studies. For example, Carreras and Andersson  found that for a sizable fraction of the cat SII neurons in their study, ipsilateral mechanical skin stimulation inhibited the response to contralateral stimulation, whereas in contrast, Picard et al.  found in their study of neurons in the distal forelimb regions of cat SII that simultaneous delivery of contralateral and ipsilateral mechanical skin stimuli led to strong facilitation of SII neuron response. In the study of Picard et al. , the responses of cells to bilateral stimulation were found to exceed the stronger of the responses to unilateral stimulation by, on average, 230%. Their study was limited, however, to the very low numbers of SII neurons that had bilateral RFs on the distal limbs. Burton, et al. , similar to Carreras and Andersson , reported that SII cells with bilateral receptive fields (monkey) exhibited a reduction in mean firing rate of 30% when the contralateral stimulus was preceded by an ipsilateral stimulus. Finally, other workers have found that callosally-transmitted inputs tend to have excitatory effects on SII neurons that have bilateral RFs, and exert inhibitory effects on SII neurons that have exclusively contralateral RFs [6–8]. Simoes et al.  showed significant suppression of the MEG SII response in humans, with simultaneous inputs delivered to the same skin sites, and Hoechstetter et al.  described "interactions" in SII cortex (a response that was not the summation of the ipsilateral and contralateral response) to simultaneous bilateral stimuli. Definitive establishment of the relationship between stimulus-evoked SII neuroelectrical and OIS activation, however, must await the performance of combined imaging and neurophysiological investigations which utilize both methodologies in the same subjects and under the same stimulus conditions.
The main, although not the only, route for ipsilateral input to SII is through the corpus callosum, from cells located in SI and SII of the opposite cerebral hemisphere [4, 7, 8, 11]. Even those regions in SII that represent most distal parts of the limbs receive significant numbers of connections from the homologous zones of the contralateral SI and SII [12–15]. Graziosi  showed that separate populations of cells in SI provide callosal projections to SI and SII in the opposite hemisphere and ipsilateral projections to SII. Some separation within SII of the responses to ipsilateral and contralateral stimulation was also shown by Friedman et al.  and Juliano et al. .
The neurons in the distal limb regions of SII do receive substantial callosal connections, but these neurons have been reported to lack ipsilateral RFs , indicating that callosal inputs are not strong enough to generate action potentials (at least under the conditions used in RF mapping studies). This suggests that SII neurons do not use their sensory inputs from the ipsilateral side of the body to construct functional properties dependent on bilateral inputs; in other words, to extract information about higher-order properties of bi-manually contacted objects from coordinated patterns of sensory stimulation of the two hands. Instead, it could be postulated that neurons in the distal limb regions of SII use their ipsilateral peripheral inputs to modulate the responses to contralateral peripheral stimulation. On the other hand, Bennett et al.  found that bilateral convergence on SII neurons varies markedly with the different classes of tactile neurons, and modulation of the SII response by ipsilateral inputs may vary from one cortical area to another with different stimulus modalities.
A number of interactions between stimuli applied to both hands have been demonstrated in human psychophysical studies. Gilson  found that the threshold for detection of vibrotactile stimuli applied to a fingertip is elevated by parallel stimulation of the other hand's fingers. In addition, Gescheider and Verrillo  reported that the magnitude of vibrotactile sensation, elicited by brief 25 or 300 Hz stimuli applied to thenar eminence, was decreased by stimuli applied simultaneously to the opposite hand, but was enhanced when the contralateral stimulus was applied 150 msec prior to the test stimuli. Essick and Whitsel  reported that the perception of the direction of motion of brushing stimuli on the skin is enhanced by the presence of a simultaneous contralateral brushing stimulus when the two stimuli move in the same direction, but is weakened when the contralateral stimulus moves in a direction opposite to that on the other arm. While the above described reports provide possible perceptual correlates for bilateral interactions that might occur in SII, such as those identified in the present study, it will remain uncertain until anterior or posterior SII cortical activity is studied under conditions that permit direct correlations of perceptual performance and cortical activity under precisely controlled conditions of contralateral vs. bilateral skin stimulation.
A recent report  demonstrated 3 separate functional cortical fields along the anterior-posterior axis in the macaque. These functional fields were defined based on differential neural responses from three distinct cortical fields, and their report was unique in that it described cortical areas within SII based on functional properties of cortical areas. In this report, we demonstrate at least two functional subdivisions within SII in the cat based on functional properties as well. However, the modes of stimulation used to distinguish the functional differences along the anterior-posterior axis of SII were very different in this study (contralateral/ipsilateral/bilateral vs. proprioceptive/cutaneous inputs in the Fitzgerald study), and subsequent investigations using other stimulus modalities could reveal that SII of the cat is organized in a very similar fashion to SII of primates. The multiple fields found in SII, based on functional differences, could be, as suggested by Fitzgerald, et al. , indicative of the existence of a number of distributed processing streams. The significance of the presented work is that the response of these different cortical areas, which could represent information from so-called separate information streams, changes in a manner dependent upon the activity of neighboring cortical areas. Distinction of cortical areas within SII, identified by functional characteristics, demonstrates the nonlinearity of the integration of information from different sources (or information streams).
One question that the results suggest is whether or not SII can be segregated by laterality preference, in a manner similar to that observed in other sensory systems. Laterality has been demonstrated in the primary sensory cortex of both the visual system and the auditory system of both primates and cats, and the data in this report strongly suggest that there are cortical areas within SII that exhibit preference to ipsilateral or contralateral inputs. In terms of processing information from simultaneous contralateral and ipsilateral stimuli, there could be further similarities between the somatosensory, auditory and visual systems that have yet to be described. Future investigations will aim to further clarify the role of SII in integration of information from inputs across the body midline.
The responses evoked by contralateral and ipsilateral flutter stimulation of the central pad of the cat forepaw define functional subdivisions in SII: the two modes of stimulation maximally activate cortical regions that are anterior and posterior to one another, respectively. Bilateral stimulation, or providing simultaneous contralateral and ipsilateral stimulation, reveals, additionally, that the two adjacent cortical areas process bilateral inputs differently. In the posterior region, where ipsilateral stimulation evokes a maximal response, bilateral stimuli evoke a response that is greater than the response evoked by either the individual ipsilateral or contralateral response. In the anterior region of SII, where the contralateral stimulus evokes a maximal response, bilateral stimuli evoke responses that are smaller in magnitude than the responses evoked by the contralateral stimulus.
Subjects & preparation
Adult cats (males and females; n = 6) were subjects. All surgical procedures were carried out under deep general anesthesia (1 – 4% halothane in a 50/50 mixture of oxygen and nitrous oxide). After induction of general anesthesia the trachea was intubated with a soft tube and a polyethylene cannula was inserted in the femoral vein to allow administration of drugs and fluids (5% dextrose and 0.9% NaCl). For each subject, a 1.5 cm diameter opening was made in the skull overlying somatosensory cortex, a chamber was mounted to the skull over the opening with dental acrylic, and the dura overlying anterior parietal cortex was incised and removed. Following the completion of the surgical procedures all wound margins were infiltrated with long-lasting local anesthetic, the skin and muscle incisions were closed with sutures, and each surgical site outside the recording chamber was covered with a bandage held in place by adhesive tape.
Subjects were immobilized with Norcuron and ventilated with a gas mixture (a 50/50 mix of oxygen and nitrous oxide; supplemented with 0.1 – 1.0% halothane when necessary) delivered via a positive pressure respirator 1–3 hours prior to the data acquisition phase of the OIS imaging experiments. Respirator rate and volume were adjusted to maintain end-tidal CO2 between 3.0 – 4.0%; EEG and autonomic signs (slow wave content; heart rate, etc.) were monitored and titrated (by adjustments in the anesthetic gas mixture) to maintain levels consistent with light general anesthesia. Rectal temperature was maintained (using a heating pad) at 37.5°C.
Euthanasia was achieved by intravenous injection of pentobarbital (45 mg/kg) and by intracardial perfusion with saline followed by fixative (10% formalin). Following perfusion fiducial marks were placed to guide removal, blocking, and subsequent histological sectioning of the cortical region studied. All procedures were reviewed and approved in advance by an institutional committee and are in full compliance with current NIH policy on animal welfare.
Stimuli and stimulus protocols
Results were obtained during stimulation of the contralateral central pad of the forepaw and/or the ipsilateral central pad of the forepaw. The stimuli always consisted of sinusoidal vertical skin displacements (25 Hz, 400 microns, stimulus duration 5 – 20 sec, inter-stimulus interval 60 sec) and were applied using a servocontrolled transducer (Cantek Enterprises, Canonsburg, PA) that is capable of delivering sinusoidal stimuli in the range of 1–250 Hz at amplitudes in the range of 0–1000 microns. The stimuli were delivered independently to the ipsilateral and contralateral skin sites, and also were applied simultaneously to both sites (bilateral stimulation). The stimulus probes were positioned 500 microns beyond the point at which skin contact was detected (via force transducer on the Cantek). The bilateral stimulus protocols reported in this paper were synchronized to start and stop at the same time. The contralateral, ipsilateral and bilateral stimuli were interleaved on a trial-by-trial basis. This approach was used to control for temporal changes in cortical "state" unrelated to stimulus conditions which, if unrecognized, might obscure or modify any differences between the optical responses evoked by the contralateral, ipsilateral and bilateral stimulus conditions.
Near-infrared (IR; 833 nm) OIS imaging was carried out using an oil-filled chamber capped with an optical window . Images of the exposed cortical surface were acquired 200 msec before stimulus onset ("reference" or "prestimulus" images) and continuously thereafter ("poststimulus" images; at a resolution of one image every 0.5 to 1.5 sec) for 15–20 sec following stimulus onset. Exposure time was 200 msec. Absorbance images were generated by subtracting each prestimulus (reference) image from its corresponding poststimulus image and subsequently dividing by the reference image. Averaged absorbance images typically show regions of both increased absorption of IR light and decreased absorption of light (to a depth of approximately 1400 microns) which have been shown to be accompanied by increases and decreases in neuronal activation, respectively [24–29].
Histological procedures/identification of cytoarchitectural boundaries
At the conclusion of the experiment, the imaged cortical region was removed immediately following intracardial perfusion with saline and fixative. The region then was blocked, postfixed, cryoprotected, frozen, sectioned serially at 30 μm, and the sections stained with cresyl fast violet. The boundaries between adjacent cytoarchitectonic areas were identified by scanning individual sagittal sections separated by no more than 300 μm and were plotted at high resolution using a microscope with a drawing tube attachment. The resulting plots then were used to reconstruct a two-dimensional surface map of the cytoarchitectonic boundaries within the region studied with optical and neurophysiological recording methods. The locations of microelectrode tracks and electrolytic lesions evident in the histological sections were projected radially to the pial surface and transferred to the map of cytoarchitectonic boundaries reconstructed from the same sections. As the final step, the cytoarchitectonic boundaries (along with the locations of microelectrode tracks and lesions whenever present) identified in each brain were mapped onto the images of the stimulus-evoked intrinsic signal obtained from the same subject, using fiducial points (made by postmortem applications of india ink or needle stabs) as well as morphological landmarks (e.g., blood vessels and sulci evident both in the optical images and in histological sections). Locations of cytoarchitectonic boundaries were identified using established criteria [30–32].
optical intrinsic signal
This work was supported, in part, by US Army Research Office grant P43077-LS (M. Tommerdahl, P.I.), NIH NS050587 (M. Tommerdahl, P.I.) and NIH NS35222 (B. Whitsel, P.I.).
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