Slowly developing depression of N-methyl-D-aspartate receptor mediated responses in young rat hippocampi
© Dozmorov et al; licensee BioMed Central Ltd. 2004
Received: 10 May 2004
Accepted: 3 August 2004
Published: 3 August 2004
Activation of N-methyl-D-aspartate (NMDA) type glutamate receptors is essential in triggering various forms of synaptic plasticity. A critical issue is to what extent such plasticity involves persistent changes of glutamate receptor subtypes and many prior studies have suggested a main role for alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors in mediating the effect. Our previous work in hippocampal slices revealed that, under pharmacological unblocking of NMDA receptors, both AMPA and NMDA receptor mediated responses undergo a slowly developing depression. In the present study we have further adressed this phenomenon, focusing on the contribution via NMDA receptors. Pharmacologically isolated NMDA receptor mediated excitatory postsynaptic potentials (EPSPs) were recorded for two independent synaptic pathways in CA1 area using perfusion with low Mg2+ (0.1 mM) to unblock NMDA receptors.
Following unblocking of NMDA receptors, there was a gradual decline of NMDA receptor mediated EPSPs for 2–3 hours towards a stable level of ca. 60–70 % of the maximal size. If such an experimental session was repeated twice in the same pathway with a period of NMDA receptor blockade in between, the depression attained in the first session was still evident in the second one and no further decay occurred. The persistency of the depression was also validated by comparison between pathways. It was found that the responses of a control pathway, unstimulated in the first session of receptor unblocking, behaved as novel responses when tested in association with the depressed pathway under the second session. In similar experiments, but with AP5 present during the first session, there was no subsequent difference between NMDA EPSPs.
Our findings show that merely evoking NMDA receptor mediated responses results in a depression which is input specific, induced via NMDA receptor activation, and is maintained for several hours through periods of receptor blockade. The similarity to key features of long-term depression and long-term potentiation suggests a possible relation to these phenomena. Additionally, a short term potentiation and decay (<5 min) were observed during sudden start of NMDA receptor activation supporting the idea that NMDA receptor mediated responses are highly plastic.
Hippocampal synapses display a variety of activity dependent changes that may represent basic elements of memory. Of foremost interest are long-term potentiation (LTP) and depression (LTD), especially forms that depend on N-methyl-D-aspartate (NMDA) receptor activation and therefore can attain "associative" properties [1–3]. The selective induction of LTP versus LTD has been attributed to differing amounts of Ca2+ ions entering via postsynaptic NMDA receptor channels . Depending on type of stimulation, enzymes with different sensitivities to Ca2+ may be engaged and change the balance between kinase and phosphatase activities, leading to either phosphorylation or dephosphorylation of postsynaptic target proteins, such as ionotropic receptors . It has been shown that afferent stimulation by frequencies in the range 0.5 to 5 Hz reliably produces LTD whereas higher frequencies, 50–100 Hz, lead to LTP . Several studies suggest that temporal factors are also important, implying that LTD requires a longer time to be induced than LTP . We have previously demonstrated that under conditions of facilitated activation of NMDA receptors by low extracellular Mg2+ synaptic plasticity can be induced by frequencies as low as 0.1–0.2 Hz when applied for prolonged periods of time . Following an initial phase of transient potentiation there was a substantial depression that developed gradually during several hours and that remained stable after termination of NMDA receptor activation. Although the relation to "standard LTD" was not fully clarified, such slowly developing depression in low Mg2+ solution may provide a useful model for studying certain forms of NMDA receptor dependent depression. In the present study, we will further develop the concept of gradually decaying responses.
One critical issue regarding LTP, LTD as well as other forms of glutamatergic synaptic plasticity, is the relative contribution of different glutamate receptor subtypes in creating the synaptic modification. Knowledge about this matter may be helpful in elucidating the underlying modification. While a selective change of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors has been cherished [8–10], especially in the case of LTP, several studies also observed NMDA receptor mediated changes in both LTP and LTD [11–14]. Previous work on LTD in our lab described an equal change of AMPA and NMDA responses . However, it was reported by others that the relative contributions of AMPA and NMDA responses during LTD depend on experimental conditions, an equal change being one possible outcome . In our recent examination of a slowly developing depression using composite AMPA-NMDA excitatory postsynaptic potentials (EPSPs) , the two responses declined in close parallel, indicating a common factor. Such an equal change is compatible with both a coordinated change of receptors and a presynaptic one via a decrease of glutamate release. However, in view of other studies reporting a coupling between responses via AMPA and NMDA receptors [16, 17], one may ask whether our observation of declining NMDA responses could be secondary to the change of AMPA. In the present study, isolated NMDA receptor mediated EPSPs were shown to decline progressively during prolonged low frequency activation (0.1 Hz). Moreover, following sudden start of stimulation there was an initial, transient potentiation. Our findings also resolved some questions regarding input specificity and durability of the slow decay, which were previously addressed only for AMPA EPSPs.
Isolated NMDA EPSPs show a progressive decay
Reinduction in a naive pathway
To exclude the possibility that the observed decay of NMDA responses were due to deterioration of slices, implying a general decrease of essential physiological processes, the experiment was repeated for the other pathway, i.e. the one that had not previously expressed NMDA responses (continuation in the same set of 8 slices). As seen in Fig. 1A (lower part), a similar result was obtained as above. The NMDA EPSP peaked at 98 ± 7 % and declined to 63 ± 8 % relative to the peak in the first experimental session. As illustrated in Fig. 1B, the curve obtained for a naive pathway during the second session of NMDA unblocking was similar to the one obtained for the pathway activated during the first session, the two curves overlapping closely for the entire recording period.
Comparison between pathways
Specificity and persistency
Instantaneous versus persistent depression
The above results suggest that the progressive decay observed in a single pathway as an instant event actually represents a long-term, pathway-specific change that can be assessed long later by comparison across pathways. To further examine the relation between the instantaneously recorded depression and the one measured about 90 min later, the relation between the two was plotted as illustrated in Fig. 3C. The two variables were found to be positively correlated (r = 0.71, p < 0.05, n = 8), implying that depression to a lower level in one pathway led to smaller responses in that pathway at later times compared to another pathway. The regression line, passing below rather than through the point of no depression (100 %, 100 %), indicates that even a slight instantaneous decay may be coupled to a noticeable change in the long term. Possibly, the declining trend was partially masked by recovery from AP5 leading to an underestimation of it.
Effect of NMDA receptor blockade on subsequent NMDA EPSP decay
To pinpoint the induction mechanism, in terms of a pre- versus postsynaptic location, experiments were carried out in a similar way as above except for keeping AP5 in the solution during the first session (see Fig. 2A). Hence, the stimulus pattern included a 3 h long interval with no stimulation in one of the pathways. The other pathway was stimulated during that time, and most likely releasing glutamate, but no postsynaptic response was expressed due to blockade of NMDA receptors. It can be argued that successful blockade of depression would predict a postsynaptic mechanism whereas a failure to do so would predict a presynaptic one. Fig. 2B shows that the depression was actually blocked, demonstrating the importance of NMDA receptor activation in the induction process. As illustrated, the two curves obtained during unblocking of NMDA receptors in the second session were quite similar. The continuously stimulated pathway, being depressed in the standard case, peaked at a level of 105 ± 5 % relative to the control pathway (n = 5; see also graphic summary of values in Fig. 3B).
Phase trajectories as indicators of waveform change
Depending on the type of synaptic modification, EPSP waveforms may change in different ways, and previous work in our lab has demonstrated that NMDA EPSPs are more prone than AMPA EPSPs to show these changes . Taking advantage of such waveform analysis, which might shed light on the underlying mechanism, we examined phase plots based on measures of the initial part and the later part of the NMDA EPSP (see Methods) on the X- and Y-axis, respectively. The curve in Fig. 3D is based on a total of 20 experiments (pathways), including some with only a single session. As shown, the phase trajectory displayed a loop indicating a difference between the effect of AP5 and the gradual decay of NMDA EPSPs. On the average the encircled area was 15 ± 2 % (p < 0.05, n = 20; scaling peak × peak as 100 %, clockwise being positive).
While our data show that the time window matters for measuring NMDA EPSPs, all the above results were qualitatively similar regardless of which window was used. It can be noted, however, that the depression of NMDA EPSPs by the end of a recording session was less pronounced using early measurements than late ones (responses attaining 72 ± 3 % vs. 61 ± 3 % of the peak value, p < 0.05, n = 20).
In 12 out of the 20 experiments, the fiber volley was well separated from the stimulus artifact allowing it to be properly measured. No significant change was detected, the end-of session value amounting 103 ± 2 % of the value at the EPSP peak (p > 0.05, not illustrated).
Short-term effects induced by sudden onset stimulation
In order to determine whether the transient potentiation had any obvious relation to the slow depression of NMDA EPSPs, the relation between the two was examined. Thus, the degree of initial potentiation was calculated by comparing the pathways just after stimulation was started and the depression was determined, as before, by comparing the end-of-session value with the peak value (see legend of Fig. 4 for further details). The two variables, illustrated by the XY-plot in Fig. 4C, were found to have no significant correlation (r = 0.35, p > 0.05).
Our study revealed a progressive decline of pharmacologically isolated NMDA EPSPs, as observed for several hours in response to low rate (0.1 Hz) activation of afferents. The decline was found to be a form of long-term synaptic depression with an induction linked to NMDA receptor activation and with an expression that was maintained through periods without such activation. Several of its basic characteristics were similar to those of conventional LTP and LTD, suggesting a possible relation to these phenomena.
Synapse specificity and NMDA-dependent induction
Decaying responses is a potential side effect in long-term, electric recording in vitro due to declining viability of biological tissue or other experimental imperfections. Such unspecific "run down" can not account for the present findings since the gradual depression of responses could be repeated in the same slice, using a previously undepressed pathway. On the other hand, if the experiment was repeated twice in the same pathway, the second occasion revealed a diminished NMDA EPSP that showed little further decay. Together, these results show that the depression is input specific and long lasting and that it can saturate. Moreover, the lack of associated changes of the fiber volley speaks against a failure of axon conductance , favoring a synaptic localization of the process.
While both pre- and postsynaptic expression mechanisms appear feasible, certain mechanisms of induction can be excluded. For instance, a decrease in probability of glutamate release due to a direct depletion of the vesicle pool is unlikely since AMPA EPSPs could be evoked for several hours without significant decay (see also [7, 20]). Even so, a use-dependent reduction of vesicle content may affect NMDA responses selectively under certain conditions by restricting "glutamate spillover" . The most critical data with respect to the induction mechanism is that a period of conditioning stimulation, normally leading to reduction of NMDA EPSPs in the same pathway later on, was ineffective if delivered during blockade of NMDA receptors. This implies that the induction of the depression requires activation of NMDA receptors, most likely postsynaptically.
Other observations of decaying NMDA EPSPs
The input specificity and NMDA dependent induction of the current depression conform with basic properties of conventional LTP and LTD . The depression might then be a case of LTD, although induced by an alternative protocol. In fact, LTD was shown to be associated with changes involving both AMPA and NMDA receptors, although the linkage between the two contributions is controversial [12, 15]. Moreover, both of the cited studies demonstrated LTD of isolated NMDA EPSPs induced by 1–2 Hz stimulation. In contrast, experiments in cultures, inducing LTD by field stimulation at a higher frequency (5 Hz), reported only AMPA receptor mediated changes . Direct interaction tests may further clarify the relation between the present depression and LTD.
Gradually decaying, NMDA receptor mediated responses were observed previously in our lab during recording of composite AMPA-NMDA EPSPs for several hours [7, 23]. Attempts to relate the decay to LTD demonstrated a weak reduction of subsequent LTD of AMPA responses suggesting at least some elements in common . In view of studies reporting forms of AMPA-NMDA coupling [16, 17], it is arguable that the studies demonstrating a decay of both components could have been influenced by the use of composite responses. In one of our studies , the observed depression of the AMPA component of composite EPSPs was verified by additional comparison between isolated AMPA EPSPs obtained under blockade of NMDA receptors. A similar verification was lacking for the depression of the NMDA response. By recording of isolated NMDA EPSPs, the present study ascertains that NMDA receptor mediated responses undergo a use-dependent depression, which is manifested in the absence of AMPA receptor activation. However, the decay was less pronounced than that reported previously for the NMDA component of composite EPSPs (average reduction to 60 % of peak as compared to 40 % in the previous study ).
While we observed that isolated NMDA EPSPs decay "spontaneously", most prior studies employing such EPSPs did not report a decay. It might be that limitations of recording time concealed the effect and cell dialysis during whole cell recording could also be a limiting factor. Actually, a recent study, recording "novel" responses under whole cell conditions, reported on decaying AMPA EPSPs but constant NMDA EPSPs . The possibility of AMPA receptor LTD under the present conditions could not be excluded as the blockade of the receptors may just conceal the effect. Further studies may help to reveal this matter.
Persistency and saturability
Standard LTP/LTD experiments compare relatively stable periods of recording before and after induction of the synaptic modification. This was not possible in the present case, since merely test stimulation evoked the decay. Therefore, comparisons were generally made between synaptic pathways subjected to different stimulus paradigms. The induction of depression in a single pathway during an initial 2 h period caused a subsequent difference between NMDA responses of the two pathways throughout a subsequent test period. The degree of initial decay was closely related to the later difference between pathways, suggesting that once depression occurred it could be maintained through periods of receptor blockade until testing was performed. Our data suggest a duration of the depression of more than 3 h after the initial induction period. This is in the range commonly referred to as "late", which is believed to involve special biochemistry such as gene expression and protein synthesis [25, 26]. Whether, the presently studied depression involves such changes remains to be determined.
The gradual depression of NMDA EPSPs was found to saturate after 2–3 h as evidenced by both single and double session experiments. This is in line with several other forms of NMDA-dependent plasticity, including LTP, LTD and chemically induced variants, which are shown to be saturable [27–29]. Whether, the saturation observed here is a "true one" at the level of expression is not known. Alternatively it could be a phenomenon at the induction level, related to weaker induction due to the diminished NMDA response.
Possible expression mechanisms
Previous work on conventionally induced LTD revealed an essential role for protein phosphatases in mediating the synaptic modification [30, 31]. Consistent with the idea that changes of AMPA receptors mediate NMDA-dependent synaptic plasticity [32, 33] it was demonstrated that certain sites of the GluR1 subunit were targeted in LTP/de-potentiation and other ones in LTD/de-depression [2, 34]. Less is known about mechanisms underlying NMDA receptor changes in LTP/LTD as well as in the current depression. A previous study in our lab recording composite EPSPs reported that LTD of the NMDA component was blocked by a phosphatase inhibitor in a similar manner as "standard LTD" . Hence, one can envisage that NMDA receptors would be controlled via dephosphorylation in a similar manner as inferred for AMPA receptors.
NMDA receptors also have a number of other regulatory sites, allowing for modulation by glycine, polyamines, calcium, and redox agents  and they have shown to be mobile as well [36–38], in keeping with the idea of mobile AMPA receptors in LTP/LTD [39, 40]. Regardless of details, additional factors are needed to stabilize the synaptic modification in the long term, perhaps via synthesis of new proteins as previously demonstrated for LTP and LTD lasting longer than about 3 h [25, 28, 41]. Changes in synaptic morphology and altered subunit composition of receptors are examples of protein synthesis dependent mechanisms that have been implied in late forms of plasticity [32, 42].
Although a postsynaptic modification appears to be the primary choice, a presynaptic one that is initiated postsynaptically is also conceivable. In previous attempts to distinguish between pre- and postsynaptic mechanisms, LTD was compared with depression caused by various pharmacological agents with respect to the ability to influence the waveform of EPSPs . While LTD in that study was found to affect isolated NMDA EPSPs in a uniform manner, i.e. no waveform change, the present data appeared to be less clear-cut. Nevertheless, the relation between early and late EPSP measurements differed for the initial AP5 washout period and the following period of actively induced depression, indicating a change in EPSP waveform. The depression therefore appeared to be distinct from a postsynaptic modification via modulation of channel gating. However, a clear test of the pre-post issue still remains. Unfortunately, the MK-801 test of release probability  does not appear useful when dealing with decaying responses as in the present case.
Short-term changes and their possible mechanisms
While the main line of experiments employed a smooth start of NMDA receptor activation following the gradual washout of AP5, another set of experiments made use of sudden activation by awaiting full washout until stimulation was started. Compared to smooth activation, there was an additional, transient potentiation that largely decayed within 20–30 stimuli. This is in accord with a previous study in hippocampal slices showing that stopping stimulation of composite AMPA-NMDA EPSPs for 10–60 min (and one case of isolated NMDA EPSP for 10 min) resulted in a transient potentiation when stimulation was resumed .
Several other studies describe decaying NMDA responses in relation to inactivation or desensitization of receptors [44, 45]. Accordingly, synaptically evoked NMDA responses in cell cultures were found to inactivate (i.e. decay) within a few minutes in much the same manner as observed here , a process shown to be triggered by postsynaptic influx of Ca2+ via the NMDA channels. Similar mechanisms of receptor desensitization/inactivation might be responsible in the present case in forming the transient phase after starting stimulation. Some details remain unexplained by this simple model, such as the biphasic character of the transient phase in terms of initial growth and subsequent decay. One can speculate that a minor LTP, or short-term potentiation, might be induced by the sudden activation of NMDA receptors and so would contribute to the initial growth, although the underlying cause is not addressed in this kind of explanation.
The above results emphasize that NMDA receptor mediated responses are highly plastic and that mere test stimulation can induce a short-term potentiation as well as a slowly developing depression that persists for several hours. The depression was input specific and saturable, and its induction required NMDA but not AMPA receptor activation in conformity with conventionally induced LTP and LTD, suggesting a relation to these phenomena. While a low Mg2+ solution was used in our case to unblock NMDA receptors, similar unblocking may occur naturally in response to depolarization. Several important issues are still not settled. Is the saturation of the NMDA EPSP depression an absolute matter or can it be overcome, leading to further down regulation and possibly silencing of synapses? Conversely, is it possible to reverse, i.e. de-depress, the change by LTP or similar processes, allowing for bidirectional control? Further research is needed to resolve these questions.
Experiments were performed on 12 to 18 day old Sprague-Dawley rats. The animals were decapitated after isoflurane (Forene) anesthesia in accordance with the guidelines of the Swedish Council for Laboratory Animals. All animal procedures were approved by the Local Ethics Committee at Göteborg University. The brain was removed and placed in an ice-cold artificial cerebrospinal fluid solution containing (in mM) NaCl 119, KCl 2.5, CaCl2 2, MgCl2 2, NaHCO3 26, NaH2PO4 1 and glucose 10, oxygenated by 95% O2, 5% CO2. The hippocampus was dissected out and transverse 400 μm thick slices were prepared by a vibratome or tissue chopper. The slices were initially kept in the same solution at room temperature for at least 60 min. As required, slices were then transferred to one or several "submerged type" recording chambers. During the experiment, slices were perfused at 30°C by a solution similar to that above except that the concentration of Mg2+ was 0.1 mM. The usage of low Mg2+ allowed for expression of NMDA receptor mediated responses.
Stimulation was delivered as 0.1 ms negative constant current pulses via monopolar tungsten electrodes. For each slice, two stimulating electrodes were placed in the apical dendritic layer of CA1 pyramidal cells on either side of the recording electrode to provide for stimulation of two separate sets of afferents. Field EPSPs were recorded by using a glass micropipette filled with 3 M NaCl (4–10 MΩ resistance). The basal test stimulus frequency was 0.1 Hz with stimuli delivered alternately to the two electrodes, successive stimuli being separated by 5 s. To test the effect of stimulus interruption, one of the two electrodes was given no stimulation during a certain time, the other one remaining stimulated at 0.1 Hz.
Recording commenced by monitoring isolated AMPA EPSPs in the presence of AP5 (50 μM) to block NMDA responses. A low concentration of CNQX (1 μM) was used to partially suppress the AMPA responses. In this way, somewhat larger stimulus strengths could be applied, suitable for evoking isolated NMDA EPSPs in the later part of the experiment. During the time of AMPA EPSP recording, the stimulus strengths were adjusted for each slice to equalize the synaptic inputs of the two pathways. This was essential for later comparison of NMDA EPSP across pathways. After obtaining a baseline of equal AMPA responses, the concentration of CNQX was raised to 10 μM which entirely blocked synaptic responces. The remaining non-synaptic response, consisting of stimulus artifact and presynaptic volley, was used to define "true zero".
To study NMDA receptor mediated responses, CNQX (10 μM) was maintained in the solution while AP5 was washed out for one or several 2 h periods, referred to as sessions in the following. In between the sessions as well as afterwards, synaptic transmission was again blocked by applying AP5 (50 μM), framing in the sessions by periods of recording non-synaptic responses. Under the sessions, various tests were made depending on the purpose of investigation. Usually one input remained silent during the first session and stimulation was not resumed until after synaptic transmission was reblocked. In another kind of experiment, the initially silent pathway was reactivated in the early part of the first session after NMDA receptors were unblocked, providing a means for sudden start of NMDA receptor activation.
Signals were amplified, filtered and transferred to a PC clone computer for on-line and off-line analysis by specially designed electronic equipment (based on an Eagle Instruments multifunction board) and own developed computer software. AMPA EPSPs were measured using an early time window (first 1.5 ms after the fiber volley) while NMDA EPSPs were measured using both an early (first 5 ms after volley) and a late (35–45 ms after artifact) time window. The late measurement was used in presenting most of the results, allowing easy comparison with previous work in our lab that estimated the NMDA component of composite EPSPs via a late measurement . Similar albeit not identical results were obtained with early and late measurements (see illustration in Fig. 3D).
Measurements were calculated by integrating the curve along the specified time window after substraction of the prestimulus baseline. All values were corrected by substracting the corresponding measurements of the non-synaptic potential obtained after total blockage of the EPSPs (except when measuring the fiber volley). The final data were quantified as relative values compared to a reference level defining 100 %. While the initial baseline formed a natural reference for AMPA responses, the choice was less obvious for NMDA responses, leading us to use the highest level of responses for one of the pathways in one of the experimental sessions (selected to make sense). Results are expressed as mean ± S.E.M. Statistical comparisons were made using Student's t-test.
Drugs were obtained from Tocris Cookson, UK; prefabricated stimulating electrodes were obtained from World Precision Instruments, FL USA, type TM33B.
alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid
excitatory postsynaptic potential
This study was supported by the Swedish R.C./medicine 05954 and the foundations of Adlerbert, Åhlen, Märtha & Gustaf Ågren, Herbert & Karin Jacobsson, Sigurd & Elsa Golje, Wilhelm & Martina Lundgren, Svenska Läkaresällskapet, Göteborgs Läkaresällskap, and Alzheimerfonden.
- Bliss TV, Collingridge GL: A synaptic model of memory: long-term potentiation in the hippocampus. Nature. 1993, 361 (6407): 31-39. 10.1038/361031a0.View ArticlePubMedGoogle Scholar
- Malenka RC, Nicoll RA: Long-term potentiation–a decade of progress?. Science. 1999, 285 (5435): 1870-1874. 10.1126/science.285.5435.1870.View ArticlePubMedGoogle Scholar
- Gustafsson B, Wigström H: Physiological mechanisms underlying long-term potentiation. Trends Neurosci. 1988, 11 (4): 156-162. 10.1016/0166-2236(88)90142-7.View ArticlePubMedGoogle Scholar
- Yang SN, Tang YG, Zucker RS: Selective induction of LTP and LTD by postsynaptic [Ca2+]i elevation. J Neurophysiol. 1999, 81 (2): 781-787.PubMedGoogle Scholar
- Dudek SM, Bear MF: Homosynaptic long-term depression in area CA1 of hippocampus and effects of N-methyl-D-aspartate receptor blockade. Proc Natl Acad Sci U S A. 1992, 89 (10): 4363-4367.PubMed CentralView ArticlePubMedGoogle Scholar
- Mizuno T, Kanazawa I, Sakurai M: Differential induction of LTP and LTD is not determined solely by instantaneous calcium concentration: an essential involvement of a temporal factor. Eur J Neurosci. 2001, 14 (4): 701-708. 10.1046/j.0953-816x.2001.01679.x.View ArticlePubMedGoogle Scholar
- Dozmorov M, Niu YP, Xu HP, Xiao MY, Li R, Sandberg M, Wigström H: Active decay of composite excitatory postsynaptic potentials in hippocampal slices from young rats. Brain Res. 2003, 973 (1): 44-55. 10.1016/S0006-8993(03)02536-8.View ArticlePubMedGoogle Scholar
- Durand GM, Kovalchuk Y, Konnerth A: Long-term potentiation and functional synapse induction in developing hippocampus. Nature. 1996, 381 (6577): 71-75. 10.1038/381071a0.View ArticlePubMedGoogle Scholar
- Kauer JA, Malenka RC, Nicoll RA: A persistent postsynaptic modification mediates long-term potentiation in the hippocampus. Neuron. 1988, 1 (10): 911-917.View ArticlePubMedGoogle Scholar
- Carroll RC, Lissin DV, von Zastrow M, Nicoll RA, Malenka RC: Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures. Nat Neurosci. 1999, 2 (5): 454-460. 10.1038/8123.View ArticlePubMedGoogle Scholar
- Clark KA, Collingridge GL: Synaptic potentiation of dual-component excitatory postsynaptic currents in the rat hippocampus. J Physiol. 1995, 482 (Pt 1): 39-52.PubMed CentralView ArticlePubMedGoogle Scholar
- Selig DK, Hjelmstad GO, Herron C, Nicoll RA, Malenka RC: Independent mechanisms for long-term depression of AMPA and NMDA responses. Neuron. 1995, 15 (2): 417-426. 10.1016/0896-6273(95)90045-4.View ArticlePubMedGoogle Scholar
- Muller D, Lynch G: Long-term potentiation differentially affects two components of synaptic responses in hippocampus. Proc Natl Acad Sci U S A. 1988, 85 (23): 9346-9350.PubMed CentralView ArticlePubMedGoogle Scholar
- Asztely F, Wigström H, Gustafsson B: The Relative Contribution of NMDA Receptor Channels in the Expression of Long-term Potentiation in the Hippocampal CA1 Region. Eur J Neurosci. 1992, 4 (8): 681-690.View ArticlePubMedGoogle Scholar
- Xiao MY, Karpefors M, Gustafsson B, Wigström H: On the linkage between AMPA and NMDA receptor-mediated EPSPs in homosynaptic long-term depression in the hippocampal CA1 region of young rats. J Neurosci. 1995, 15 (6): 4496-4506.PubMedGoogle Scholar
- Bazhenov AV, Kleshchevnikov AM: Reciprocal inhibition of the AMPA and NMDA components of excitatory postsynaptic potentials in field CA1 of the rat hippocampus in vitro. Neurosci Behav Physiol. 1999, 29 (6): 719-725.View ArticlePubMedGoogle Scholar
- Watt AJ, van Rossum MC, MacLeod KM, Nelson SB, Turrigiano GG: Activity coregulates quantal AMPA and NMDA currents at neocortical synapses. Neuron. 2000, 26 (3): 659-670. 10.1016/S0896-6273(00)81202-7.View ArticlePubMedGoogle Scholar
- Xiao MY, Niu YP, Wigström H: Comparing long-term depression with pharmacologically induced synaptic attenuations in young rat hippocampi. Synapse. 1997, 26 (4): 329-340. 10.1002/(SICI)1098-2396(199708)26:4<329::AID-SYN1>3.3.CO;2-U.View ArticlePubMedGoogle Scholar
- Thinschmidt JS, Walker DW, King MA: Chronic ethanol treatment reduces the magnitude of hippocampal LTD in the adult rat. Synapse. 2003, 48 (4): 189-197. 10.1002/syn.10203.View ArticlePubMedGoogle Scholar
- Xiao MY, Niu YP, Wigström H: Activity-dependent decay of early LTP revealed by dual EPSP recording in hippocampal slices from young rats. Eur J Neurosci. 1996, 8 (9): 1916-1923.View ArticlePubMedGoogle Scholar
- Kullmann DM, Asztely F: Extrasynaptic glutamate spillover in the hippocampus: evidence and implications. Trends Neurosci. 1998, 21 (1): 8-14. 10.1016/S0166-2236(97)01150-8.View ArticlePubMedGoogle Scholar
- Bear MF, Malenka RC: Synaptic plasticity: LTP and LTD. Curr Opin Neurobiol. 1994, 4 (3): 389-399. 10.1016/0959-4388(94)90101-5.View ArticlePubMedGoogle Scholar
- Niu YP, Xiao MY, Karpefors M, Wigström H: Potentiation and depression following stimulus interruption in young rat hippocampi. Neuroreport. 1999, 10 (5): 919-923.View ArticlePubMedGoogle Scholar
- Xiao MY, Wasling P, Hanse E, Gustafsson B: Creation of AMPA-silent synapses in the neonatal hippocampus. Nat Neurosci. 2004, 7 (3): 236-243. 10.1038/nn1196.View ArticlePubMedGoogle Scholar
- Sajikumar S, Frey JU: Anisomycin inhibits the late maintenance of long-term depression in rat hippocampal slices in vitro. Neurosci Lett. 2003, 338 (2): 147-150. 10.1016/S0304-3940(02)01400-3.View ArticlePubMedGoogle Scholar
- Frey U, Huang YY, Kandel ER: Effects of cAMP simulate a late stage of LTP in hippocampal CA1 neurons. Science. 1993, 260 (5114): 1661-1664.View ArticlePubMedGoogle Scholar
- Dudek SM, Bear MF: Bidirectional long-term modification of synaptic effectiveness in the adult and immature hippocampus. J Neurosci. 1993, 13 (7): 2910-2918.PubMedGoogle Scholar
- Frey U, Schollmeier K, Reymann KG, Seidenbecher T: Asymptotic hippocampal long-term potentiation in rats does not preclude additional potentiation at later phases. Neuroscience. 1995, 67 (4): 799-807. 10.1016/0306-4522(95)00117-2.View ArticlePubMedGoogle Scholar
- Lee HK, Kameyama K, Huganir RL, Bear MF: NMDA induces long-term synaptic depression and dephosphorylation of the GluR1 subunit of AMPA receptors in hippocampus. Neuron. 1998, 21 (5): 1151-1162. 10.1016/S0896-6273(00)80632-7.View ArticlePubMedGoogle Scholar
- Mulkey RM, Endo S, Shenolikar S, Malenka RC: Involvement of a calcineurin/inhibitor-1 phosphatase cascade in hippocampal long-term depression. Nature. 1994, 369 (6480): 486-488. 10.1038/369486a0.View ArticlePubMedGoogle Scholar
- Mulkey RM, Herron CE, Malenka RC: An essential role for protein phosphatases in hippocampal long-term depression. Science. 1993, 261 (5124): 1051-1055.View ArticlePubMedGoogle Scholar
- Luscher C, Nicoll RA, Malenka RC, Muller D: Synaptic plasticity and dynamic modulation of the postsynaptic membrane. Nat Neurosci. 2000, 3 (6): 545-550. 10.1038/75714.View ArticlePubMedGoogle Scholar
- Abraham WC, Williams JM: Properties and mechanisms of LTP maintenance. Neuroscientist. 2003, 9 (6): 463-474. 10.1177/1073858403259119.View ArticlePubMedGoogle Scholar
- Lee HK, Barbarosie M, Kameyama K, Bear MF, Huganir RL: Regulation of distinct AMPA receptor phosphorylation sites during bidirectional synaptic plasticity. Nature. 2000, 405 (6789): 955-959. 10.1038/35016089.View ArticlePubMedGoogle Scholar
- McBain CJ, Mayer ML: N-methyl-D-aspartic acid receptor structure and function. Physiol Rev. 1994, 74 (3): 723-760.PubMedGoogle Scholar
- Tovar KR, Westbrook GL: Mobile NMDA receptors at hippocampal synapses. Neuron. 2002, 34 (2): 255-264. 10.1016/S0896-6273(02)00658-X.View ArticlePubMedGoogle Scholar
- Carroll RC, Zukin RS: NMDA-receptor trafficking and targeting: implications for synaptic transmission and plasticity. Trends Neurosci. 2002, 25 (11): 571-577. 10.1016/S0166-2236(02)02272-5.View ArticlePubMedGoogle Scholar
- Snyder EM, Philpot BD, Huber KM, Dong X, Fallon JR, Bear MF: Internalization of ionotropic glutamate receptors in response to mGluR activation. Nat Neurosci. 2001, 4 (11): 1079-1085. 10.1038/nn746.View ArticlePubMedGoogle Scholar
- Man HY, Ju W, Ahmadian G, Wang YT: Intracellular trafficking of AMPA receptors in synaptic plasticity. Cell Mol Life Sci. 2000, 57 (11): 1526-1534.View ArticlePubMedGoogle Scholar
- Bredt DS, Nicoll RA: AMPA receptor trafficking at excitatory synapses. Neuron. 2003, 40 (2): 361-379. 10.1016/S0896-6273(03)00640-8.View ArticlePubMedGoogle Scholar
- Huang YY, Kandel ER: Recruitment of long-lasting and protein kinase A-dependent long-term potentiation in the CA1 region of hippocampus requires repeated tetanization. Learn Mem. 1994, 1 (1): 74-82.PubMedGoogle Scholar
- Williams JM, Guevremont D, Kennard JT, Mason-Parker SE, Tate WP, Abraham WC: Long-term regulation of N-methyl-D-aspartate receptor subunits and associated synaptic proteins following hippocampal synaptic plasticity. Neuroscience. 2003, 118 (4): 1003-1013. 10.1016/S0306-4522(03)00028-9.View ArticlePubMedGoogle Scholar
- Hessler NA, Shirke AM, Malinow R: The probability of transmitter release at a mammalian central synapse. Nature. 1993, 366 (6455): 569-572. 10.1038/366569a0.View ArticlePubMedGoogle Scholar
- Zilberter Y, Uteshev V, Sokolova S, Khodorov B: Desensitization of N-methyl-D-aspartate receptors in neurons dissociated from adult rat hippocampus. Mol Pharmacol. 1991, 40 (3): 337-341.PubMedGoogle Scholar
- Rosenmund C, Feltz A, Westbrook GL: Calcium-dependent inactivation of synaptic NMDA receptors in hippocampal neurons. J Neurophysiol. 1995, 73 (1): 427-430.PubMedGoogle Scholar
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