Nerve growth factor selectively regulates expression of transcripts encoding ribosomal proteins
© Angelastro et al; licensee BioMed Central Ltd. 2002
Received: 29 November 2001
Accepted: 28 February 2002
Published: 28 February 2002
NGF exerts a variety of actions including promotion of neuronal differentiation and survival. The PC12 rat pheochromocytoma cell line has proved valuable for studying how NGF works and has revealed that the NGF mechanism includes regulation of gene expression. Accordingly, we used SAGE (Serial Analysis of Gene Expression) to compare levels of specific transcripts in PC12 cells before and after long-term NGF exposure. Of the approximately 22,000 transcripts detected and quantified, 4% are NGF-regulated by 6-fold or more. Here, we used database information to identify transcripts in our SAGE libraries that encode ribosomal proteins and have compared the effect of NGF on their relative levels of expression.
Among the transcripts detected in our SAGE analysis, 74 were identified as encoding ribosomal proteins. Ribosomal protein transcripts were among the most abundantly expressed and, for naive and NGF-treated PC12 cells, represented 5.2% and 3.5%, respectively, of total transcripts analyzed. Surprisingly, nearly half of ribosomal protein transcripts underwent statistically significant NGF-promoted alterations in relative abundance, with changes of up to 5-fold. Of the changes, approximately 2/3 represented decreases. A time course revealed that the relative abundance of transcripts encoding RPL9 increases within 1 hr of NGF treatment and is maximally elevated by 8 hr.
These data establish that NGF selectively changes expression of ribosomal protein transcripts. These findings raise potential roles for regulation of ribosomal protein transcripts in NGF-promoted withdrawal from the cell cycle and neuronal differentiation and indicate that regulation of individual ribosomal protein transcripts is cell- and stimulus-specific.
Nerve growth factor (NGF), a prototypical neurotrophic factor and a member of the neurotrophin family, promotes a wide range of responses in its target cells. These range from neuronal differentiation, maintenance of survival, and regulation of metabolic activities [for review see [1–3]. Many of these actions include and require transcriptional regulation [4, 5]. However, the greater part of the changes in gene expression that underlie the NGF response remain to be elucidated. The PC12 line of rat pheochromocytoma cells  has proved to be a particularly favorable system for detecting NGF-responsive changes in gene expression [7–11]. These cells resemble sympathico-pheochromo-blasts and upon exposure to NGF cease proliferation and acquire, in a transcription dependent mechanism, many of the properties of post-mitotic sympathetic neurons including neurite outgrowth and electrical excitability. The robust nature of the response of PC12 cells to NGF coupled with their capacity to be examined both before and at various times after exposure to the factor has greatly facilitated study of the NGF mechanism of action, including gene regulation .
To obtain a comprehensive and quantitative over-view of NGF-promoted gene regulation, we  have used SAGE (Serial Analysis of Gene Expression). In this technique [13–15] cellular transcripts are converted to SAGE "tags" which are sequenced, quantified and, in many cases, matched with known genes. By comparing SAGE profiles for cells in different states (as for example before and after exposure to NGF), it is thus possible to obtain a comprehensive view of gene expression and regulation. Moreover, if a sufficient number of SAGE tags are analyzed, changes in expression levels of individual transcripts can be associated with a high level of statistical significance .
Initial analysis of approximately 157,000 SAGE tags from PC12 cells cultured without or with NGF for 9 days revealed nearly 800 transcripts (of a total of at least 21,000) that are regulated by ± 6-fold or greater in response to NGF . Of these, approximately 150 were assignable to named genes of known functions that regulate cellular behaviors ranging from actin and microtubule cytoskeleton assembly/disassembly, gene transcription, RNA processing, neurotransmission, and energetics. A variety of criteria supported the reliability of the quantitative findings revealed by our SAGE analysis .
In the present study, we have extended our SAGE profiling of naïve and long-term NGF-treated PC12 cells to over 163,000 tags representing over 22,000 unique transcripts. Analysis of these reveals the presence of transcripts encoding 74 different ribosomal proteins (RPs). Surprisingly, we find that long-term NGF exposure leads to statistically significant changes in relative abundance of at least half of these transcripts by factors of up to nearly 5-fold. In addition, a time course for one of the RP transcripts (encoding RP L9) reveals that its relative abundance begins to change within 1 hr and is maximally regulated by 8 hr of NGF exposure.
SAGE libraries were generated and analyzed as previously described  from matched sets of PC12 cells before and after 9 days of exposure to NGF. The present analysis is based on 76,280 15-mer tags from NGF-untreated PC12 cells and 87,004 tags from NGF-treated cells (after exclusion of duplicate ditags, mitochondrial transcripts and repetitive elements). Consideration of tags observed twice or more between the two libraries indicated the presence of approximately 22,000 unique transcripts. Of these, approximately 10% were regulated by more than 3-fold in response to NGF and approximately 4% by 6-fold or more.
Detection and quantification of transcripts encoding ribosomal proteins
Transcripts represented by SAGE tags were identified by direct matches of tags with the appropriate sequences of known rat genes (present in GenBank) or through matches of tags with appropriate sequences of rat ESTs (present in NCBI Unigene) that were in turn found to overlap with known rat genes. Positive identification required that the transcript or EST have a poly adenylation signal and poly A tail, and that the tag followed the most 3' CATG of the transcript. In this way 74 tags were unambiguously assignable to known rat transcripts encoding proteins described as ribosomal components. Available sequences encoding rat ribosomal proteins L15 and S8 do not contain a CATG sequence and hence SAGE tags for these could not be identified. In addition, rat sequence data for transcripts encoding RPs L2, L25, L33 and S1 are not presently available in GenBank or Unigene.
The effect of NGF (9 days treatment) on expression levels of transcripts for various ribosomal proteins in PC12 cell cultures
FOLD CHANGE +NGF
40 KD RP
Relative expression of transcripts for ribosomal proteins
The most abundantly expressed ribosomal transcripts in NGF-untreated PC12 cells included those encoding ribosomal proteins S15, S24 and L41. These each accounted for approximately 0.2–0.35% of total cell transcripts. The high relative abundance of these ribosomal transcripts in NGF-untreated cells can be appreciated by the observation that of the 4 tags encountered more than 200 times in our analysis, 2 (S15 and S24) encoded ribosomal proteins; moreover, RP transcripts represented 6 of the 13 tags encountered from 100–200 times and 23 of the 36 tags encountered from 50–100 times.
The data in Table 1 and Figure 1 show that NGF treatment resulted in an overall decrease in relative abundance of transcripts encoding RPs (from 5.2% to 3.5% of total). This may reflect in part our observation that NGF treatment increases the numbers of transcripts representing low-abundance genes . Nevertheless, transcripts for ribosomal proteins remained among the most highly expressed in the NGF-treated cells. For instance, tags for RPs L13A, L31, L37A, S24 and S29 had relative abundances of 0.1% or more. Of the 56 most abundantly expressed tags in NGF-treated cells, 21 represent ribosomal transcripts.
NGF selectively regulates the expression of transcripts encoding ribosomal proteins
Figure 2 shows the distribution of fold-changes in transcript levels in response to NGF. 47 of the 74 RP transcripts were either invariant or showed changes of 2-fold or less; 10 individual transcripts decreased by 3–5 fold (L12, L18, L24, L28, L30, S15, S16, S18, S19 and S24) and one was elevated by more than 3-fold (L17).
Rapid regulation of an RP transcript by NGF
NGF regulates expression of RP transcripts
In the present work, we used SAGE profiling to identify and quantify the relative numbers of transcripts for 74 ribosomal proteins in rat PC12 cells before and after long-term exposure to NGF. This has permitted us to detect selective changes in transcripts encoding specific RPs.
A number of criteria support the reliability of our findings. The first regards the assignment of SAGE tags to specific RP transcripts. We required that all matching ESTs or transcripts have clearly definable poly A tails and poly adenylation signals. Due to the extensive data base for rat RP transcripts, it was possible to make all matches with rat sequences. In addition, we used 15 mer (CATG +11) base SAGE tags for our analysis; we have reported  that this leads to a significantly more reliable matching of SAGE tags to genes than with the often-used 14-mer tags. A second indicator of reliability stems from prior analysis of our SAGE libraries and data obtained from them . For instance, we have shown that our SAGE libraries show little or no NGF-promoted changes in tag numbers for transcripts encoding a number of housekeeping proteins. Moreover, a number of transcripts previously found by alternative technologies to respond to NGF treatment, showed similar changes in our SAGE profiling study. Finally, Northern blot analysis verified SAGE-predicted responses of over 20 transcripts to NGF treatment. A third criterion for reliability was that Monte Carlo simulation indicated that many of the NGF-promoted responses of RP transcripts were at a probability of P < 0.01. Because of the relatively high abundance of many RP transcripts, even comparably small changes in expression could be detected at this level of significance. A last criterion was that we used real time RT-PCR to verify effects of NGF on five RP transcripts.
A previous study by Lee et al. , based on comparison of a total of approximately 7,000 random ESTs from naïve and NGF-treated PC12 cells, reported an NGF-promoted increase in expression of RPL7 transcripts and a decrease in RPL19 transcripts. This contrasts with the current data which revealed no significant change in expression of either of these transcripts. The reason for this discrepancy is unclear, but could in part originate from the relatively small number of transcripts that were surveyed in the former study. To our knowledge, there are currently no other reports regarding effects of NGF on levels of ribosomal transcripts.
Relative levels of RP transcripts
Our observations indicate that there is a wide range in the numbers transcripts per cell that encode individual RPs. For instance, both before and after NGF treatment, there was over a 10-fold difference in the relative numbers of transcripts for RPs L7, L22, S21 as compared to those encoding RP S29. Because of the general scarcity of antisera prepared against mammalian RPs, we do not know whether this is reflected at the protein level. However, for a number of other NGF-regulated transcripts in PC12 cells, there is a good correlation between relative levels of message and protein [11, 16, 17]. Thus, it may be that although levels of individual RPs are assumed to be similar to one another, some may be present in limiting numbers. Alternatively, there may be a considerable disparity between relative abundances of RP transcripts and their corresponding proteins
A recent SAGE study of targets for N-myc in a human neuroblastoma cell line , reported relative abundances for 66 RP transcripts. The existence of such data permit us to compare the relative levels of RP transcripts in two cell types (neuroblastoma and pheochromocytoma) of related origin (i.e., neural-crest-derived) that both have the potential for neuronal differentiation. The 74 RP transcripts detected here represented 5.2% of the total transcripts in NGF-untreated PC12 cells and 3.5% after NGF treatment. By contrast, the 66 RPs reported in the neuroblastoma study accounted for 4.1% of total transcripts for cells without N-myc over-expression and 12.6% for such cells transfected with N-myc. Thus, for non-N-myc transfected neuroblastoma cells and PC12 cells (± NGF) the contribution of total RP transcripts lies in a similar range and this parameter is greatly elevated in neuroblastoma cells by N-myc over-expression. With respect to transcripts for individual RP's, there are many similarities as well as several striking differences between the two cell types. When NGF-untreated PC12 cells are compared with non-transfected neuroblastoma cells, of the 55 RP transcripts detected in common for both systems, about half (a total of 28) have relative abundances within a factor of 2. Twenty transcripts are more than 3-fold higher in relative abundance in PC12 cells. In two of these cases (S9 and S17) no tags were detected in the non-transformed neuroblastoma cells; in several other cases (S12, S24, L24) the relative abundance in PC12 cells was over 20-fold that in the neuroblastoma cells. For most of these, expression of N-myc elevated the neuroblastoma levels to relative abundances within 2-fold of those in PC12 cells. However, for L24 and S24, the relative abundance in N-myc-expressing neuroblastoma cells was still 1/3 that in PC12 cells. Finally 7 RP transcripts (L7, L11, L21, L30, S7, S8 and P2) were of 3–5 times lower abundance in PC12 cells than in neuroblastoma cells and this difference was appreciably enhanced when the NB cells were transfected with N-myc.
We have also compared our RP results with those for SAGE carried out with pooled human adult brain (tissue supplied by Gregory J. Riggins: http://www.ncbi.nlm.nih.gov/sage/sagerec.cgi?rec=161). Comparison with data for NGF-treated cells reveals similarity in relative abundance within a factor of 2 for 39 of the 57 RP transcripts in common between the libraries and 48 of 57 to within a factor of 3. However, several large differences do occur. For example the relative abundances of transcripts encoding RPs L9, L39, S24, S13, and S17 are 5–10-fold higher in NGF-treated PC12 cells than in the pooled brain library and transcripts for RP L28 are 8-fold lower. These observations reinforce the notion that expression of individual RP transcripts can be significantly variable from cell type to cell type as well as sensitive to extrinsic signals.
Potential significance of RP transcript regulation
Although the ribosome has been considered as a "molecular machine" , it is of interest that the transcripts encoding individual proteins of this organelle are subject to regulation by NGF. Two related questions emerge regarding these findings: how do these changes compare with previous reports for regulation of RP transcripts and what might be the functional consequences of these changes?
A number of studies have employed a variety of techniques to detect changes in gene expression associated with the oncogenic state and have reported selective elevation of specific RP transcripts in tumors. Examples include RPs L7a, L37 and S14 in prostate tumors and cell lines ; RPs L5, L7A, L18, S3, S6, S8, S12, S13, S28, P0 and P1 in colorectal cancers and cell lines [21–26]; L5 in astrocytomas ; L18a in squamous cell carcinoma ; L19 in breast tumors that over-express erbB-2 ; RPs S3A, S4, and S17 in lymphoid malignancies ; L38, S4, P0, and P1 in rhabdomyosarcoma cell lines ; and P0, P1, P2, L5, L9, L35, L39, S3A, S10, and S17 in liver tumors . In addition, as noted above, a recent SAGE study identified targets of N-myc in a human neuroblastoma cell line . Of 114 up-regulated genes detected, 66 encoded RPs with elevations ranging from 40% to 37-fold. Several of these genes were also up-regulated by c-myc. Taken together, these findings indicate that the transition to the transformed state is associated with elevation of various RP transcripts. A potential interpretation of such observations is that this up-regulation reflects the enhanced rate of cell division in tumor cells and the requirement for greater levels of protein synthesis. In agreement with this possibility, transcripts encoding RPs L6 and S7 are reported to be up-regulated in regenerating liver . However, for the study involving responses of neuroblastoma cells to N-Myc, despite the massive up-regulation of RP transcripts, there was no over-all increase in the rate of protein synthesis .
Before considering potential functional consequences of the changes in RP expression described here as well as elsewhere, it must be conceded that changes in transcript abundance may not necessarily lead to changes in protein expression. The present lack of available antibodies/antiserum to most mammalian RPs hampers such a determination. However, even if cases occur in which altered RP transcript expression does not lead to changes in expression of the corresponding protein, our and others' findings regarding selective regulation of RP transcripts would then raise the interesting issue as to why, and the mechanism by which, transcript and protein expression are uncoupled.
If at least some of the NGF-promoted alterations in RP transcript abundance lead to changes in expression of the corresponding proteins, what might be the functional consequences? In the present system, NGF converts proliferating PC12 cells to a non-proliferating neuronally differentiated state. Our findings reveal that the relative overall abundance of transcripts for RP proteins fell by 1/3 in response to NGF. In addition, nearly half of the individual RP transcripts detected showed significant changes in expression and approximately 2/3 of these were decreases. This overall decrease in NGF-promoted RP transcript expression and the preponderance of decreases in expression of specific RP transcripts compared to increases would favor the interpretation that the changes observed here reflect, at least in part, the transition to the non-dividing phenotype. On the other hand, we observed that many of the RP transcripts did not show significant responses to NGF and that a number showed increases in expression. This suggests that the situation is likely to be more complex with individual RPs perhaps playing specific roles not simply associated with the state of cell proliferative capacity. In agreement with this, we found no significant change in expression of RP S29 transcripts even though these have been reported to be present at low levels in growth phase cells and elevated in quiescent cells .
In addition to leaving the cell cycle, NGF-treated PC12 cells undergo neuronal differentiation. This raises the possibility that some of the observed changes, as for many other NGF regulated genes, is related to acquisition of the neuronal phenotype. An analysis of gene regulation in human NTERA2 cells induced to leave the cell cycle and neuronally differentiate in response to retinoic acid revealed decreases in RP transcripts including L3, L7, L8, L10, L13, L39, S2, S6, S13, S16, S20, S19, S23, S27A and P0 . Of these, In the present study only RP transcripts L8 and S6, S16, S19 showed significant down regulation in response to NGF whereas the others found in the NTERA2 study either did not show changes that were significant at the P ≤ .01 level or underwent a significant increase (RP S27A). Curcic et al.  reported that differentiation of BC3H1 myocytes is accompanied by a drop in L32 gene transcription; in contrast, we found no significant change in transcripts encoding this RP. In another study, RPs L35a and S5 were down regulated during murine erythryolukemia cell differentiation ; we also observed decreases in these transcripts. Mutation of RP S19 is associated with Blackfan's anemia and therefore appears to play a selective role in differentiation/proliferation of erythropoetic cells . Here, NGF resulted in a nearly 5-fold drop in S19 transcripts. Thus, although changes in expression of specific RP genes may be associated with differentiation, the pattern of such changes may reflect the particular cell type and differentiation stimulus that is involved.
Another condition associated with changes in RP expression is apoptotic death. RP L4/L1 transcripts, which were not significantly affected by NGF, are selectively upregulated in PC12 cells prior to 5 aza cytosine-induced death and over-expression of this gene in COS-7 cells induces apoptosis . Over-expression of L7, L13A, S29 have been also reported to induce apoptosis [40, 41]. In the present study, NGF promoted little if any change in expression of these RP transcripts. Thus, although NGF is an effective anti-apoptotic factor, these actions do not appear to be mediated by down-regulation of potentially death-inducing RPs.
At present, in contrast to studies on bacterial and archael ribosomes, relatively little is known about the functions of individual mammalian ribosomal proteins (. However, assuming that changes in message abundance lead to alterations in protein levels, it is reasonable to anticipate that the NGF-promoted effects observed here may affect the protein synthetic capacity of the cell in some manner. Such changes are unlikely to be global; NGF does affect the cellular rate of protein synthesis, but this seems to be due at least in part to post-translational modification of the translational machinery . The alternative is that the changes reported here may result in selective effects on translation of specific messages.
A final, and important possibility to consider is that a number of RPs appear to possess extraribosomal functions  and consequently that the changes reported here may lead to responses that do not directly relate to ribosomal function. For example, RP L18, which was significantly down-regulated by NGF, has been reported to negatively regulate double-stranded RNA (dsRNA)-activated protein kinase (PKR) . As an additional example RP S19, which is also down-regulated by NGF, has been implicated in erythropoiesis and has also been described as a monocyte chemotactic factor . As one last example, rat RP L9 which we found to be up-regulated by NGF starting within a few hours and maximally by 8 hrs, has been shown to be the ortholog of the yeast gene grc5 which appears to be involved in multiple cellular functions including growth control, cytoskeleton control and energy metabolism . Identification of NGF-regulated RPs should now facilitate exploration of their potential roles in the responses of cells to neurotrophins.
SAGE analysis provides a reliable, quantitative picture of ribosomal protein expression in PC12 cells before and after long term exposure to NGF.
Transcripts for ribosomal proteins are among the most abundant transcripts in the cells; however there is a wide range between numbers of transcripts for individual RP transcripts.
NGF promotes an overall decrease in relative RP transcript expression (for the 74 RP transcripts detected, from approximately 5% of total transcripts to 3.5%). This drop represents a decrease in relative expression of individual RP transcripts as well as an increase in message complexity in NGF-treated cells.
Long term NGF treatment of PC12 cells promotes statistically significant changes in expression of over half of the transcripts encoding ribosomal proteins. Decreases in expression outnumbered increases by a ratio of approximately 2:1. The largest observed changes in expression are in the range of 3–5 fold.
For at least one RP transcript (RPL9) the response to NGF is rapid; an elevation is detected within 1 hr of NGF exposure and reaches maximum regulation by 8 hrs.
Examination of the literature reveals many other reports in which expression of individual or groups of RP transcripts are regulated in response to growth factors, differentiation agents or malignant transformation. Comparison with the present data indicate that changes in ribosomal protein transcripts is regulated in a cell and state dependent fashion with a large diversity in the particular RP transcripts that are subject to regulation. Thus, although the ribosome may be regarded as a "machine" there is a good deal of potential for plasticity with respect to the expression of its various components. This raises the possibilities that growth factors and other cell regulators may affect ribosomal function, and thereby the capacity of cells to transcribe specific transcripts.
In the absence of information about the specific functions of most individual RPs in mammalian cells, one can only speculate at present on the physiologic significance of the reported changes. However, it seems highly plausible that the observed responses may play roles in NGF-promoted neuronal differentiation. This may be mediated in part by selective effects on translation of certain messages. In addition, past findings support the possibility that at least some of the regulated RPs have extra-ribosomal actions that may affect neuronal differentiation and function.
Materials and Methods
SAGE and matching SAGE tags with RP transcripts
SAGE libraries were prepared from naïve and 9-day NGF-treated PC12 cells and sequenced as previously described . To match SAGE tags with RP transcripts, tags were initially analyzed with the National Center for Biotechnology Information (NCBI) rat SAGE tag to gene mapping database ftp://ncbi.nlm.nih.gov/pub/sage/map/Rn/Nla3, which matches possible 14-mer tags with known rat genes and expressed sequence tags (ESTs). With the use of sequences present in the NCBI UNIGENE rat database http://www.ncbi.nlm.nih.gov/UniGene/Rn.Home.html potential matches were further scrutinized to determine whether there was a match at the 15th base and to determine whether the matched sequence was at the most 3' end of a known rat transcript or EST. We considered only cases in which a clear poly(A) tail and a polyadenylation signal were present at the 3' end of the transcript or EST. Appropriate ESTs were further analyzed by an advanced BLAST search for matches with known rat RP genes. Monte-Carlo simulation analysis of the SAGE data was carried out using the SAGE 300 software package .
Real time quantitative PCR analysis
PC12 cells were cultured as previously described  in complete medium (85% RPMI 1640 medium, 10% horse serum, 5% fetal bovine serum). Replicate cultures were treated with 50 ng/ml rhNGF (kindly donated by Genentech, Inc.) for the indicated times. Total cellular RNA was isolated as described previously  and 1.2 – 5 μg was used for reverse transcription with 5'-T30NN-3' primer using Superscript II Rnase H-RT according to the manufacturer's specifications (Life Technologies, Grand Island, NY). Quantitative real time PCR reactions were performed as described by Troy et al. . GAPDH was used to normalize input cDNA for samples originating from short-term NGF treatments and β-actin was used for this purpose for samples with long-term NGF exposure.
Forward and reverse primers pairs used for quantitative PCR were: S29, 5'GGTATCACAGGGTAGACAGT3' and 5'GGTATCACAGGGTAGACAGT3'; L9, 5'GAACTCTGAGGAGGGACTTC3' and 5'AAACTGTACTTGTTATCAGGAT3'; S27-1, 5'CGGCACGAGCGACCTCCCTA3' and 5'GTTCCCACTCATCTTGAATC3'; L24, 5'CAAGAAAGGACAGTCGGAAA3' and 5'TTCACAGGCTTCACAATCTT3'; S19, 5'TAACCAGCAGGAGTTCGTCA3' and 5'TTTGTTCTAATGCTTCTTGTT3'; β-Actin, 5'ATCCTGACCCTGAAGTACCC3' and 5'TACGACCAGAGGCATACAG3'; and GAPDH, 5'GAAACCTGCCAAGTATGATGA3' and 5'TCTCTCTTGCTCTCAGTATCC3', respectively.
We thank Claudine Bitel for her excellent technical assistance. Supported in part by grants from the National Institutes of Health.
- Levi-Montalcini R: The saga of the nerve growth factor. Neuroreport. 1998, 9: R71-83.PubMedGoogle Scholar
- Frade JM, Barde YA: Nerve growth factor: two receptors, multiple functions. Bioessays. 1998, 20: 137-145. 10.1002/(SICI)1521-1878(199802)20:2<137::AID-BIES6>3.3.CO;2-A.View ArticlePubMedGoogle Scholar
- Tessarollo L: Pleiotropic functions of neurotrophins in development. Cytokine Growth Factor Rev. 1998, 9: 125-137. 10.1016/S1359-6101(98)00003-3.View ArticlePubMedGoogle Scholar
- Greene LA, Shooter EM: The nerve growth factor: biochemistry, synthesis, and mechanism of action. Annu Rev Neurosci. 1980, 3: 353-402. 10.1146/annurev.ne.03.030180.002033.View ArticlePubMedGoogle Scholar
- Segal RA, Greenberg ME: Intracellular signaling pathways activated by neurotrophic factors. Annu Rev Neurosci. 1996, 19: 463-489.View ArticlePubMedGoogle Scholar
- Greene LA, Tischler AS: Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci USA. 1976, 73: 2424-2428.PubMed CentralView ArticlePubMedGoogle Scholar
- Leonard DG, Ziff EB, Greene LA: Identification and characterization of mRNAs regulated by nerve growth factor in PC12 cells. Mol Cell Biol. 1987, 7: 3156-3167.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee NH, Weinstock KG, Kirkness EF, Earle-Hughes JA, Fuldner RA, Marmaros S, Glodek A, Gocayne JD, Adams MD, Kerlavage AR, et al: Comparative expressed-sequence-tag analysis of differential gene expression profiles in PC-12 cells before and after nerve growth factor treatment. Proc Natl Acad Sci USA. 1995, 92: 8303-8307.PubMed CentralView ArticlePubMedGoogle Scholar
- Vician L, Basconcillo R, Herschman HR: Identification of genes preferentially induced by nerve growth factor versus epidermal growth factor in PC12 pheochromocytoma cells by means of representational difference analysis. J Neurosci Res. 1997, 50: 32-43. 10.1002/(SICI)1097-4547(19971001)50:1<32::AID-JNR4>3.0.CO;2-M.View ArticlePubMedGoogle Scholar
- Brown AJ, Hutchings C, Burke JF, Mayne LV: Application of a rapid method (targeted display) for the identification of differentially expressed mRNAs following NGF-induced neuronal differentiation in PC12 cells. Mol Cell Neurosci. 1999, 13: 119-130. 10.1006/mcne.1999.0736.View ArticlePubMedGoogle Scholar
- Angelastro JM, Klimaschewski L, Tang S, Vitolo OV, Weissman TA, Donlin LT, Shelanski ML, Greene LA: Identification of diverse nerve growth factor-regulated genes by serial analysis of gene expression (SAGE) profiling. Proc Natl Acad Sci USA. 2000, 97: 10424-10429. 10.1073/pnas.97.19.10424.PubMed CentralView ArticlePubMedGoogle Scholar
- Greene LA, Farinelli SE, Cunningham ME, Park DS: Culture and experimental use of the PC12 rat pheochromocytoma cell line. In Culturing Nerve Cells. Edited by: Banker F, Goslin K. 1998, Cambridge, MA, MIT Press, 161-187. 2Google Scholar
- Velculescu VE, Zhang L, Vogelstein B, Kinzler KW: Serial analysis of gene expression. Science. 1995, 270: 484-487.View ArticlePubMedGoogle Scholar
- Velculescu VE, Vogelstein B, Kinzler KW: Analysing uncharted transcriptomes with SAGE. Trends Genet. 2000, 16: 423-425. 10.1016/S0168-9525(00)02114-4.View ArticlePubMedGoogle Scholar
- Yamamoto M, Wakatsuki T, Hada A, Ryo A: Use of serial analysis of gene expression (SAGE) technology. J Immunol Methods. 2001, 250: 45-66. 10.1016/S0022-1759(01)00305-2.View ArticlePubMedGoogle Scholar
- Aletta JM, Angeletti R, Liem RK, Purcell C, Shelanski ML, Greene LA: Relationship between the nerve growth factor-regulated clone 73 gene product and the 58-kilodalton neuronal intermediate filament protein (peripherin). J Neurochem. 1988, 51: 1317-1320.View ArticlePubMedGoogle Scholar
- Bai G, Weiss B: The increase of calmodulin in PC12 cells induced by NGF is caused by differential expression of multiple mRNAs for calmodulin. J Cell Physiol. 1991, 149: 414-421.View ArticlePubMedGoogle Scholar
- Boon K, Caron HN, van Asperen R, Valentijn L, Hermus MC, van Sluis P, Roobeek I, Weis I, Voute PA, Schwab M, Versteeg R: N-myc enhances the expression of a large set of genes functioning in ribosome biogenesis and protein synthesis. EMBO. 2001, 20: 1383-1393. 10.1093/emboj/20.6.1383.View ArticleGoogle Scholar
- Frank J: The ribosome-a macromolecular machine par excellence. Chem Biol. 2000, 7: R133-R141. 10.1016/S1074-5521(00)00127-7.View ArticlePubMedGoogle Scholar
- Vaarala MH, Porvari KS, Kyllonen AP, Mustonen MV, Lukkarinen O, Vihko PT: Several genes encoding ribosomal proteins are over-expressed in prostate-cancer cell lines: confirmation of L7a and L37 over-expression in prostate-cancer tissue samples. Int J Cancer. 1998, 78: 27-32. 10.1002/(SICI)1097-0215(19980925)78:1<27::AID-IJC6>3.3.CO;2-A.View ArticlePubMedGoogle Scholar
- Pogue-Geile K, Geiser JR, Shu M, Miller C, Wool IG, Meisler AI, Pipas JM: Ribosomal protein genes are overexpressed in colorectal cancer: isolation of a cDNA clone encoding the human S3 ribosomal protein. Mol Cell Biol. 1991, 11: 3842-3849.PubMed CentralView ArticlePubMedGoogle Scholar
- Puder M, Barnard GF, Staniunas RJ, Steele GD, Chen LB: Nucleotide and deduced amino acid sequence of human ribosomal protein L18. Biochim Biophys Acta. 1993, 216: 134-136.View ArticleGoogle Scholar
- Denis MG, Chadeneau C, Lecabellec MT, LeMoullac B, LeMevel B, Meflah K, Lustenberger P: Over-expression of the S13 ribosomal protein in actively growing cells. Int J Cancer. 1993, 55: 275-280.View ArticlePubMedGoogle Scholar
- Cao J, Cai X, Zheng L, Geng L, Shi Z, Pao CC, Zheng S: Characterization of colorectal-cancer-related cDNA clones obtained by subtractive hybridization screening. J Cancer Res Clin Oncol. 1997, 123: 447-451. 10.1007/s004320050085.View ArticlePubMedGoogle Scholar
- Wang Y, Cheong D, Chan S, Hooi SC: Ribosomal protein L7a gene is up-regulated but not fused to the tyrosine kinase receptor as chimeric trk oncogene in human colorectal carcinoma. Int J Oncol. 2000, 16: 757-762.PubMedGoogle Scholar
- Parle-McDermott A, McWilliam P, Tighe O, Dunican D, Croke DT: Serial analysis of gene expression identifies putative metastasis-associated transcripts in colon tumour cell lines. Br J Cancer. 2000, 83: 725-728. 10.1054/bjoc.2000.1330.PubMed CentralView ArticlePubMedGoogle Scholar
- Huang H, Colella S, Kurrer M, Yonekawa Y, Kleihues P, Ohgaki H: Gene expression profiling of low-grade diffuse astrocytomas by cDNA arrays. Cancer Res. 2000, 60: 6868-6874.PubMedGoogle Scholar
- Dong G, Loukinova E, Smith CW, Chen Z, Van Waes C: Genes differentially expressed with malignant transformation and metastatic tumor progression of murine squamous cell carcinoma. J Cell Biochem Suppl. 1997, 28: 90-100. 10.1002/(SICI)1097-4644(1997)28/29+<90::AID-JCB10>3.3.CO;2-B.View ArticlePubMedGoogle Scholar
- Henry JL, Coggin DL, King CR: High-level expression of the ribosomal protein L19 in human breast tumors that overexpress erbB-2. Cancer Res. 1993, 53: 1403-1408.PubMedGoogle Scholar
- Starkey CR, Levy LS: Identification of differentially expressed genes in T-lymphoid malignancies in an animal model system. Int J Cancer. 1995, 62: 325-331.View ArticlePubMedGoogle Scholar
- Daigneault L, Beaulieu R, Filion M, Gaboury L, Royal A, Babai F: Cloning and identification of genes differentially expressed in metastatic and non-metastatic rat rhabdomyosarcoma cell lines. Clin Exp Metastasis. 1995, 13: 345-356.View ArticlePubMedGoogle Scholar
- Shuda M, Kondoh N, Tanaka K, Ryo A, Wakatsuki T, Hada A, Goseki N, Igari T, Hatsuse K, Aihara T, Horiuchi S, Shichita M, Yamamoto N, Yamamoto M: Enhanced expression of translation factor mRNAs in hepatocellular carcinoma. Anticancer Res. 2000, 20: 2489-249.PubMedGoogle Scholar
- Xu W, Wang S, Wang G, Wei H, He F, Yang X: Identification and characterization of differentially expressed genes in the early response phase during liver regeneration. Biochem Biophys Res Commun. 2000, 278: 318-325. 10.1006/bbrc.2000.3792.View ArticlePubMedGoogle Scholar
- Coppock D, Kopman C, Gudas J, Cina-Poppe DA: Regulation of the quiescence-induced genes: quiescin Q6, decorin, and ribosomal protein. Biochem Biophys Res Commun. 2000, 269: 604-610. 10.1006/bbrc.2000.2324.View ArticlePubMedGoogle Scholar
- Bevort M, Leffers H: Down regulation of ribosomal protein mRNAs during neuronal differentiation of human NTERA2 cells. Differentiation. 2000, 66: 81-92. 10.1046/j.1432-0436.2000.660203.x.View ArticlePubMedGoogle Scholar
- Curcic D, Glibetic M, Larson DE, Sells BH: GA-binding protein is involved in altered expression of ribosomal protein L32 gene. J Cell Biochem. 1997, 65: 287-307. 10.1002/(SICI)1097-4644(19970601)65:3<287::AID-JCB1>3.0.CO;2-O.View ArticlePubMedGoogle Scholar
- Pappas IS, Vizirianakis IS, Tsiftsoglou AS: Cloning, sequencing and expression of a cDNA encoding the mouse 135a ribosomal protein during differentiation of murine erythroleukemia (MEL) cells. Cell Biol Int. 2001, 25: 629-634. 10.1006/cbir.2000.0695.View ArticlePubMedGoogle Scholar
- Matsson H, Klar J, Draptchinskaia N, Gustavsson P, Carlsson B, Bowers D, de Bont E, Dahl N: Truncating ribosomal protein S19 mutations and variable clinical expression in Diamond-Blackfan anemia. Hum Genet. 1999, 105: 496-500. 10.1007/s004390051136.View ArticlePubMedGoogle Scholar
- S Kajikawa, H Nakayama, M Suzuki, A Takashima, O Murayama, M Nishihara, M Takahashi, K Doi: Increased expression of rat ribosomal protein L4 mRNA in 5-azacytidine-treated PC12 cells prior to apoptosis. Biochem Biophys Res Commun. 1998, 252: 220-224. 10.1006/bbrc.1998.9633.View ArticleGoogle Scholar
- Chen FW, Ioannou YA: Ribosomal proteins in cell proliferation and apoptosis. Int Rev. Immunol. 1999, 18: 429-448.View ArticlePubMedGoogle Scholar
- Khanna N, Reddy VG, Tuteja N, Singh N: Differential gene expression in apoptosis: identification of ribosomal protein S29 as an apoptotic inducer. Biochem Biophys Res. Commun. 2000, 277: 476-486. 10.1006/bbrc.2000.3688.View ArticlePubMedGoogle Scholar
- Lafontaine DL, Tollervey D: The function and synthesis of ribosomes. Nat. Rev. Mol. cell biol. 2001, 2: 514-520. 10.1038/35080045.View ArticlePubMedGoogle Scholar
- Kleijn M, Welsh GI, Scheper GC, Voorma HO, Proud CG, Thomas AA: Nerve and epidermal growth factor induce protein synthesis and eIF2B activation in PC12 cells. J Biol Chem. 1998, 273: 5536-5541. 10.1074/jbc.273.10.5536.View ArticlePubMedGoogle Scholar
- Wool IG: Extraribosomal functions of ribosomal proteins. Trends Biochem Sci. 1996, 21: 164-165. 10.1016/0968-0004(96)20011-8.View ArticlePubMedGoogle Scholar
- Kumar KU, Srivastava SP, Kaufman RJ: Double-stranded RNA-activated protein kinase (PKR) is negatively regulated by 60S ribosomal subunit protein L18. Mol Cell. Biol. 1999, 19: 1116-1125.PubMed CentralView ArticlePubMedGoogle Scholar
- Nishiura H, Tanase S, Sibuya Y, Nishimura T, Yamamoto T: Determination of the cross-linked residues in homo-dimerization of S19 ribosomal protein concomitant with exhibition of monocyte chemotactic activity. Lab Invest. 1999, 79: 915-923.PubMedGoogle Scholar
- Nika J, Erickson FL, Hannig EM: Ribosomal protein L9 is the product of GRC5, a homolog of the putative tumor suppressor QM in S. cerevisiae. Yeast. 1997, 13: 1155-1166. 10.1002/(SICI)1097-0061(19970930)13:12<1155::AID-YEA166>3.0.CO;2-O.View ArticlePubMedGoogle Scholar
- Troy CM, Rabacchi SA, Hohl JB, Angelastro JM, Greene LA, Shelanski ML: Death in the balance: alternative participation of the caspase-2 and -9 pathways in neuronal death induced by nerve growth factor deprivation. J. Neurosci. 2001, 21: 5007-5016.PubMedGoogle Scholar
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