670 nm light mitigates oxygen-induced degeneration in C57BL/6J mouse retina
© Albarracin et al.; licensee BioMed Central Ltd. 2013
Received: 23 November 2012
Accepted: 18 September 2013
Published: 17 October 2013
Irradiation with light wavelengths from the far red (FR) to the near infrared (NIR) spectrum (600 nm -1000 nm) has been shown to have beneficial effects in several disease models. In this study, we aim to examine whether 670 nm red light pretreatment can provide protection against hyperoxia-induced damage in the C57BL/6J mouse retina. Adult mice (90–110 days) were pretreated with 9 J/cm2 of 670 nm light once daily for 5 consecutive days prior to being placed in hyperoxic environment (75% oxygen). Control groups were exposed to hyperoxia, but received no 670 nm light pretreatment. Retinas were collected after 0, 3, 7, 10 or 14 days of hyperoxia exposure (n = 12/group) and prepared either for histological analysis, or RNA extraction and quantitative polymerase chain reaction (qPCR). Photoreceptor damage and loss were quantified by counting photoreceptors undergoing cell death and measuring photoreceptor layer thickness. Localization of acrolein, and cytochrome c oxidase subunit Va (Cox Va) were identified through immunohistochemistry. Expression of heme oxygenase-1 (Hmox-1), complement component 3 (C3) and fibroblast growth factor 2 (Fgf-2) genes were quantified using qPCR.
The hyperoxia-induced photoreceptor loss was accompanied by reduction of metabolic marker, Cox Va, and increased expression of oxidative stress indicator, acrolein and Hmox-1. Pretreatment with 670 nm red light reduced expression of markers of oxidative stress and C3, and slowed, but did not prevent, photoreceptor loss over the time course of hyperoxia exposure.
The damaging effects of hyperoxia on photoreceptors were ameliorated following pretreatment with 670 nm light in hyperoxic mouse retinas. These results suggest that pretreatment with 670 nm light may provide stability to photoreceptors in conditions of oxidative stress.
Keywords670 nm light irradiation Near infrared Photobiomodulation Hyperoxia Retinal degeneration Neuroprotection Cytochrome oxidase Oxidative stress Retinal inflammation Oxygen toxicity
Irradiation with light wavelengths from the far red (FR) to the near infrared (NIR) spectrum (600 nm -1000 nm) has been shown to have beneficial effects in mammalian tissues [1–5]. Red light therapies have been used to treat several disease conditions in humans and in animal models for over 30 years including treatment of soft tissue injuries, diabetic wounds, radiation-induced ulcers, inflammatory and neurodegenerative conditions [6–11].
In the last 10 years, there has been an increasing interest in the use of 670 nm red light to manage retinal injuries. Early reports demonstrated the ability of 670 nm red light to attenuate damaging effects of methanol intoxication in rat retinas and laser-induced injury in primate retinas [12, 13]. More recent studies have shown that 670 nm light treatment provides significant protection to photoreceptors in retinas exposed to damaging levels of light [14–16]. We have previously reported that 670 nm light treatment is effective in maintaining retinal function, and in significantly reducing expression of markers of inflammation and oxidative stress in rat retinas damaged by bright white light exposure [15–17]. Although the underlying mechanism of its therapeutic effect remains controversial, current evidence suggests that 670 nm light has a direct effect on the key mitochondrial enzyme, the cytochrome c oxidase (CCO). Upon absorption of 670 nm wavelength light, the activity of CCO increased along with energy production in the form of ATP [18, 19]. Although unproven, this process is also thought to stimulate signalling pathways resulting in improved mitochondrial energy metabolism, antioxidant production, cell survival and regulation of non-coding RNAs [17, 20].
Prolonged exposure to an elevated concentration of oxygen (hyperoxia) is toxic to a number of tissues in the respiratory and central nervous systems [21, 22]. In the retina, early investigations in neonatal and adult rabbits and in adult mice demonstrated that exposure to hyperoxia can cause severe damage to photoreceptors [23–25]. The most commonly-observed pathological effects in the hyperoxia-induced mouse model are photoreceptor and endothelial cell death, increased inflammation, oxidative stress, erosion of the blood-retina barrier (BRB) and loss of functional vision [26, 27, 29–31]. These changes are site-specific, such that the irreversible loss of photoreceptors is most prominent in the circumscribed area of the inferior retina, approximately 500 μm from the optic disk .
Although the precise mechanism for the photoreceptor vulnerability to hyperoxia is not completely understood, it is postulated to be initiated and sustained by a “toxic cycle” of events , involving hyperoxia-induced oxidative stress in the outer retina through generation of free radicals and reactive oxygen species (ROS). Given the ineffective or lack of autoregulatory mechanism for the oxygen supply in the outer retina , prolonged exposure to hyperoxia will inevitably cause an increase in ROS production by the photoreceptors. Generation of ROS will subsequently trigger various pathological changes, such as oxidative damage, particularly in photoreceptors. These light sensitive cells contain a large number of polyunsaturated fatty acid (PUFA), that are also highly sensitive to ROS . The hyperoxia-induced depletion of photoreceptors can also contribute to the rise of oxygen tension, ROS accumulation which in turn leads to further cell death, and progression of degeneration. These changes are also featured in the later stages of Age-related Macular Degeneration (AMD) and Retinitis Pigmentosa (RP) [35, 36], suggesting that hyperoxia may be a suitable model for investigating novel rescue strategies to slow down the progression of these diseases.
In this study we aimed to examine the effects of pretreatment with 670 nm light on levels of oxidative stress, apoptosis and inflammatory response in the hyperoxia-induced retinopathy in adult mouse retina.
TUNEL (cell death) analysis
The quantitative analyses for the average total number of TUNEL + photoreceptors in NT and Tr animals at different timepoints are summarized in Figure 6. The data show a sharp increase in TUNEL + cells associated with increased period of exposure to hyperoxia in NT retinas (3d to 14d, black bars). Although there is also an increase in TUNEL + cells in the Tr animals over the same period, TUNEL + cells only begin to accumulate in significant numbers until 7d exposure, and there are significantly fewer TUNEL + cells in Tr animals at each timepoint.
Metabolic profile and oxidative stress
We used antibodies against Cox Va and acrolein to qualitatively and quantitatively evaluate the effect of 670 nm pretreatment on retinal metabolism and oxidative stress in animals exposed to hyperoxia. Because the damage resulting from hyperoxic exposure occurs mainly in a ‘hotspot’ in the inferior retina these analyses were carried out using images taken from that region. To further assess the level of oxidative stress, we used real time quantitative PCR to measure the heme oxygenase-1 (Hmox-1) mRNA expression.
Retinal metabolic status (Cox Va)
Oxidative stress status (acrolein)
Baseline immunoreactivity to acrolein was determined from control retinas (0d; Figure 8B, red). A 3-fold increase in acrolein immunoreactivity above baseline was observed in 3dNT retinas (Figure 8B, black bar), increasing to 20-fold at 7d, 36-fold at 10dNT and 37-fold in 14dNT. In contrast, there was no significant increase of acrolein immunoreactivity in 3dTr retinas above baseline (Figure 8B, red); after 7 days of exposure to hyperoxia there was a 20-fold increase in acrolein immunoreactivity above baseline, comparable with 7dNT retinas. However, acrolein immunoreactivity was maintained at this level at the later timepoints in the Tr groups (10dTr and 14dTr).
Expression of Hmox-1, C3 and Fgf-2 genes were analysed by quantitative RT-PCR to monitor the hyperoxia-induced stress responses of the NT and Tr retinas.
Expression of C3 increased over longer periods of hyperoxia exposure (Figure 10B) in both Tr and NT animals. In the NT group (black bars), this increase above baseline was significant at 7d and 14d. At 10d and 14d, there were significantly higher levels of C3 expression in NT retinas (black bar) compared to Tr retinas (red bars) (p < 0.05).
A small and not statistically significant upregulation of Fgf-2 was detected at 3d in both Tr (Figure 10C, red bar) and NT animals (Figure 9C, black bar). After 7d hyperoxia, there was a 10-fold up-regulation of Fgf-2 in the NT retinas, compared with a 26-fold upregulation in Tr animals. Levels of expression were significantly different between 7dTr and 7dNT animals (p < 0.05). Levels of Fgf-2 continued to increase in NT animals at 10d and 14d; however, in Tr animals there was a fall in Fgf-2 expression at 10d to approximately 15-fold above baseline, which was maintained at 14d in the Tr group. At 10d and 14d, expression levels of Fgf-2 in NT retinas were higher compared to Tr retinas, showing significant difference at 14d (p < 0.05).
These findings corroborate earlier studies showing that hyperoxia causes oxidative stress, photoreceptor-specific cell death, and retinal inflammation [30, 32, 38]. Those studies show that (i) hyperoxia-induced changes are evident by 3d exposure to hyperoxia, (ii) that there is a focal point of pathology in the inferior central retina, and (iii) this pathology spreads to other regions of the retina over time.
In addition, the present findings show that pretreatment with 670 nm light effectively offsets photoreceptor death induced by oxidative stress. The data demonstrate that pretreatment with 9 J/cm2 of 670 nm once daily for 5 consecutive days in this model: (i) delays the onset, and slows the progression of photoreceptor death, and (ii) downregulates the associated oxidative stress markers, acrolein and Hmox-1. This decrease in oxidative stress leads to the attenuation of damage to the retina and the reduction of expression of proinflammatory C3 expression and reduced expression of the stress-inducible neuroprotectant, Fgf-2. Significantly, treatment of 670 nm light alone did not cause measurable damage in non-challenged retinas.
670 nm light treatment promotes photoreceptor survival
The timecourse of photoreceptor degeneration in this model has been reported previously . In the present study, we find that the severity of the hyperoxia-induced damage in photoreceptors is significantly reduced, but not prevented, by pretreatment with 670 nm red light. We used two methods to quantify damage to photoreceptors: measures of ONL thickness, and counts of numbers of apoptotic (TUNEL positive) photoreceptors. Analyses of ONL thickness, the cumulative measure of cell loss, indicates that 14dTr animals have an ONL thickness profile approximating that of 10dNT animals (Figure 2), suggesting that in this model 9 J/cm2, 670 nm light treatment slows hyperoxia-induced degeneration by around 4 days. Counts of apoptotic photoreceptors (Figures 4 and 5) along the vertical meridian, a definitive indicator of the rate of photoreceptor loss, support this interpretation. The levels of cell death in 14dTr and 10dNT retinas; 10dTr and 7dNT were almost identical (Figures 4, 5 and 6), suggesting that 670 nm light delays progression of hyperoxia-induced cell death.
670 nm light attenuates oxidative damage
Hyperoxic exposure was previously demonstrated to induce oxidative stress, which differentially affects photoreceptors [31, 38, 39]. The high PUFA content, increased oxygen tension in the outer retina, and the continuous exposure to light render the photoreceptors vulnerable to oxidative stress . The present data confirms those observations and indicates that irradiation with 670 nm light ameliorates oxidative stress such that there are minimal changes in cytochrome oxidase immunoreactivity, and lower levels of expression of the oxidative stress indicators, acrolein and Hmox-1.
Acrolein, the by-product of lipid peroxidation, and the stress-inducible Hmox-1 gene are both reliable indicators for oxidative stress and have been linked to a range of degenerative conditions, including AMD [37, 41]. In the present study, we observed changes in acrolein (Figure 8B) and Hmox-1 (Figure 10A) expression profiles indicative of oxidative stress at 7 days of hyperoxia exposure (both NT and Tr). At 10 and 14 days, the NT retinas continued to show upregulation of these markers, implicating progression of oxidative stress. However, in the treated retinas in these time points (10dTr and 14dTr) there were no further increases in acrolein and Hmox-1 expression levels. In addition, the intensity of immunolabelling for cytochrome oxidase in these retinas remained unchanged, suggesting that 670 nm light treatment maintains mitochondrial metabolism, leading to the attenuation of hyperoxia-induced oxidative damage.
Mitochondrial dysfunction increases oxidative stress, and has been implicated in the development of several retinal degenerative conditions including AMD, diabetic retinopathy and glaucoma [42–45]. Therefore, any treatment strategy aimed at preventing oxidative stress may prove beneficial in slowing the progression of retinal degeneration. Several reports showed that 670 nm light treatment reduces oxidative damage, including a rat in vivo model of methanol-induced retinal toxicity  and animals exposed to damaging bright light [16, 17]. In the latter study, the levels of expression of the oxidative damage indicators, Hmox-1 and inducible nitric oxide synthase  were downregulated following 670 nm light treatment. Similarly, it has been reported that transcranial delivery of 670 nm light attenuates secondary oxidative damage in rat optic nerve following partial transection . Moreover, findings from studies of Parkinson’s disease [5, 47] and Multiple Sclerosis  also show that treatment with 670 nm light mitigates oxidative stress in these experimental models. Taken together, these observations provide strong evidence that 670 nm light ameliorates oxidative damage in disorders where oxidative stress plays a key role in the progression of pathology.
670 nm light mitigates C3 expression
The present study finds that exposure to hyperoxia causes a 20-fold upregulation of the complement component gene C3 in NT retinas, by 10d exposure; this upregulation occurs in conjunction with high levels of expression of the oxidative stress markers, and increased rate of cell death (Figure 5). However, in the treated retinas there was a much smaller rise in C3 expression (5-fold in 10dTr retinas, Figure 10B). This finding is consistent with reports from studies carried out in aged rodents  and in rats exposed to damaging bright light [15, 17].
Dysregulation of C3 plays a highly significant role in retinal diseases, and dysregulation of complement is highly associated with risk of AMD [49, 50, 52]. Several lines of evidence suggest that oxidative stress triggers complement activation [53, 54], which in turn mediates local inflammation. Tissue damage may result from cell-mediated attack, or by the formation of membrane attack complex, which kills cells. The low levels of C3 expression in the treated retinas in this study, suggest that 670 nm light is a potential therapeutic tool applicable to retinal degenerative conditions where inflammation is involved, such as AMD.
670 nm light treatment reduces retinal damage
Several studies of retinal degeneration report an increase in expression of neuroprotective factors such as Fgf-2 over time, which protects the retina by delaying, or in some cases, reducing photoreceptor cell death [51, 55–57]. It has been reported previously that exposing the retina to a hyperoxic environment induces changes in the expression of Fgf-2[29, 55]. The current study also demonstrates that Fgf-2 expression is modulated by 670 nm light in hyperoxic conditions, although the effect is complex.
In this model, cell death in the ONL increases over time spent in hyperoxia, and at the 7d timepoint there are high levels of cell death in the NT group, and only low levels of cell death in the Tr group (Figures 5 and 6). Consistent with a role in neuroprotection, Fgf-2 expression is higher in the 7dTr group (low levels of cell death) compared with the 7dNT group (Figure 10C). However, Fgf-2 expression is significantly lower in 10dTr animals (compared with 10dNT) which is consistent with a finding that by 10d exposure to hyperoxia there are high levels of cell death, even in the retinas of treated animals. Beyond this timepoint Fgf-2 expression remains approximately static. The explanation for the lowering of levels of Fgf-2 in 10dTr and 14dTr is not immediately apparent, but may reflect the limitations of the efficacy of 670 nm light in offsetting the effects of hyperoxia. However, the reasons why 670 nm light treatment reaches a plateau, or declines, at this stage cannot be explained until we have a better understanding of its mechanism of action.
670 nm light in hyperoxia-induced retinal degeneration
The present findings indicate that the mechanism of action of 670 nm light is likely to involve signalling pathways activated by oxidative stress in the retina. It is generally accepted that oxidative stress initiates and facilitates the progression of hyperoxia-induced damage in the central nervous system and in the retina [31, 58]. High oxygen tension is believed to induce metabolic disturbances in the PUFA-rich photoreceptor outer segments that trigger formation of ROS . Accumulation of ROS leads to oxidative stress, and in turn to inflammation, and ultimately loss of functional vision [59, 60]. Exposure of the retina to 670 nm light has been suggested to initiate key signalling pathways that activate transcription factors  to modulate pro/anti-oxidant balance in tissues , inflammatory responses [16, 17] and cytoprotection [12–15]. The present results support those findings in that the data indicates that 670 nm light treatment limits oxidative stress and proinflammation, reducing retinal stress and cell death.
Several reports have suggested the important role of mitochondria in mediating the effects of 670 nm light irradiation [18, 28]. First, the mitochondrial cytochrome oxidase has been proposed as the endogenous primary photoacceptor molecule in the reception of 670 nm light triggering secondary cellular processes such as enhanced energy-rich adenosine triphosphate (ATP) synthesis and other various modulatory effects [18, 20]. Second, 670 nm light was suggested to influence retrograde signalling between the mitochondria and the nucleus, resulting in enhanced synthesis of DNA and RNA . Third, the intra-mitochrondrial activities of nitric oxide (NO) and the cytochrome oxidase have recently been proposed as a novel mechanism of action of 670 nm light [62, 63]. NO controls oxygen consumption of the tissue through reversible and competitive binding to cytochrome oxidase . In a recent review, Poyton and Ball provided a mechanistic explanation suggesting that 670 nm light may cause dissociation of NO from the cytochrome oxidase complex allowing NO to bind effectively to oxygen during tissue damage .
Here, we have demonstrated that hyperoxia-induced oxidative stress, cell death, retinal stress and inflammation were significantly mitigated, but not abolished, by 670 nm light treatment. Oxygen toxicity has been shown to cause oxidative stress and inflammation triggering photoreceptor cell death which are also implicated in the late stages of retinal degenerative conditions in humans, such as AMD, RP and Retinopathy of Prematurity (ROP) [66–68]. Hence, 670 nm red light treatment has the potential to slow the progression of retinopathies and conditions involving oxidative stress.
All procedures were in accordance with the Association for Research in Vision and Ophthalmology (ARVO) Statement for the Use of Animals in Ophthalmic and Vision Research, and with the requirements of The Australian National University Animal Experimentation Ethics Committee. C57BL/6J mice aged P90 (n = 12/group) of mixed sexes were reared in low (5 lx) light levels with a 12 h light, 12 h dark cycle. Food and water were available ad libitum.
Oxygen damage (hyperoxia) and tissue collection
Animals were divided into 2 groups: 670 nm light-treated (Tr) and nontreated (NT). All experimental groups contained equal proportions of male and female C57BL/6J mice in all experiments. The treated animals received 670 nm light treatment prior to being placed in a clear plastic chamber, and exposed to 75% ± 0.5% oxygen, maintained using a computer-controlled feedback device (Oxycycler; Biospherix, Lacona, NY). The nontreated animals were placed in the oxygen chamber the same time as the 670 nm light-treated groups. Oxygen exposure commenced at 9:05 AM in all experimental groups. The animals were sacrificed and tissues were collected at 5 time points 0 day (0d, control), 3 days (3d), 7 days (7d), 10 days (10d) and 14 days (14d) exposure to 75% oxygen. Control animals were sacrificed at 0d (no O2) following 670 nm light treatment (Treated Controls), or without the 670 nm light treatment (Nontreated Controls). Tissues were collected at 9:00 AM of each time points.
670 nm pretreatment paradigm
All experimental groups (NT and Tr) were transferred into a clear plexiglass. The Tr groups were taken into the treatment room prior to 670 nm light treatment procedure. The 670 nm LED array (Quantum Device, WI, USA) was positioned so that the mouse eyes were approximately 2.5 cm away from the light source. Animals were exposed to 670 nm light for 3 minutes, once daily at 9:00 AM, for 5 consecutive days at 60 mW/cm2 delivering an energy fluence of 9 J/cm2 at eye level. Mice from the NT groups were transferred into separate room while the Tr animals were undergoing treatment.
Experimental groups nomenclature
The nontreated animals are designated with the following group names: 0dNT, 3dNT, 7dNT, 10dNT and 14dNT. The 0dNT animals were not exposed to hyperoxia and did not receive 670 nm red light treatment. The 670 nm light-treated groups of each time point are designated as follows: 0dTr, 3dTr, 7dTr, 10dTr and 14dTr. The animals from 0dTr group were not exposed to hyperoxia but did receive 670 nm light-pretreatment.
Mice were sacrificed by cervical dislocation. Excised retinas from the right eye of each animal were collected and stored in RNAlater® (Ambion, Applied Biosystems, Foster City, CA) overnight at 4°C for quantitative RT-PCR. Left eyes were marked at the superior aspect of the limbus for orientation, enucleated and immersion-fixed in 4% paraformaldehyde in 0.1 M phosphate-buffered saline (PBS) at pH 7.4 for 2 hours. Tissue was rinsed thrice in 0.1 M PBS and left in a 15% sucrose solution overnight for cryoprotection. The next day, eyes were embedded in Tissue-Tek OCT Compound (Sakura Finetek, Tokyo, Japan), and snap frozen in liquid nitrogen. The eyes were cryosectioned at 16 μm thickness, in the sagittal plane, to allow a dorsal to ventral observation of the retina using Leica CM1850 cryostat (Leica Microsystems, Nossloch, Germany). Sections were mounted on gelatin and poly-L-lysine-coated slides and dried overnight at 50°C before being stored at -20°C.
Detection of cell death using TUNEL assay
Cryosectioned C57BL/6J mouse retinas from the NT and Tr groups at all timepoints were stained with TdT-mediated dUTP nick end labelling (TUNEL) using an established protocol . To identify the layers of the retina, DNA-specific dye Bisbenzimide (BBZ) Hoechst (1:1000, Calbiochem, CA) was used. Quantitative assessment of cell death was conducted using parameters outlined in the following section.
Retinal morphometric analyses
To quantify the level of hyperoxia-induced photoreceptor cell loss and apoptosis in the nontreated and treated groups, we used 2 methods: outer nuclear layer (ONL) thickness measurement and counts of photoreceptor nuclei density and TUNEL-positive (+) cells. Measurements of ONL thickness and counts of photoreceptor nuclei density were done in four main areas of the retina (Figure 1A); the inferior periphery (IP), the inferior central (IC), superior central (SC) and superior periphery (SP), using digital photomicrographs taken from the bisbenzimide-stained sections. In at least 4 retinal sections per animal, 10 measurements of ONL per section were obtained. To account for obliquely cut retina, we measured and recorded the thickness of the ONL, as well as the thickness of the retina, from the inner to the outer limiting membrane (OLM-ILM). The ratio of the ONL to the OLM-ILM was used for analysis. ImageJ software (National Institute of Health) was used to count photoreceptor nuclei density across the four main areas of the retina. The values obtained were normalised against the number of photoreceptor nuclei in the nontreated control retinas (0dNT) and the results were expressed in percentages. Secondly, the number of TUNEL + cells in the photoreceptor layer was counted across the retina, from inferior to superior, and either expressed per unit area and/or total number of TUNEL + cells, in 3 sections per animal. Counts of TUNEL + cells in control animals were used to determine baseline levels of cell death. Results from 12 animals from each group were averaged and analysed.
Immunohistochemistry (IHC) was performed using standard protocols for fluorescent imaging. Briefly, frozen sections of the retina were placed in 70% ethanol followed by 2 rinses in 0.1 M of PBS. All sections were permeabilised with an antigen retrieval solution (Immunosolution Pty Ltd, Australia) for 45 mins at 37°C, followed by blocking with 10% normal goat serum (Sigma Aldrich, St Louis, MO, USA) for 1 h before incubation with the primary antibody for 24 h at 4°C. Primary antibodies used are mouse monoclonal antibody against cytochrome oxidase Va (1:100; Mitosciences, USA) and rabbit polyclonal antibody against acrolein (1:500; Cell Sciences, USA). Following rinses with 0.1 M PBS, sections were treated with a secondary antibody of either rabbit IgG conjugated with Alexa Fluor 594 or mouse IgG conjugated with Alexa Fluor 488 (1:1000, Molecular Probes, OR, USA) for 24 h at 4°C before incubation with the DNA-specific dye bisbenzimide Hoechst (1:10 000) for 2 min. Slides were coverslipped using a mixture of glycerol gelatin (Sigma, St Louis, MO, USA) and water.
Confocal and light microscopy analyses
Sections were examined, scanned and analysed using Carl Zeiss LSM 5 Pascal confocal microscope (Germany), LM Zeiss Apotome (Germany) and Zeiss Axioplan. During image collection, the photomultiplier settings and exposure times for each fluorescent channel were kept constant to allow comparison of protein levels between sections.
Quantitative measurement on signal intensity of immunofluorescent sections
Digital images of the acrolein and Cox Va-labelled sections obtained from confocal microscopy were processed and analysed using ImageJ software. Consistency in the analyses was ensured by using identical parameters with respect to section thickness (z-stack), areas sampled for analysis, antibody concentrations, duration of incubation times, and microscope configuration.
Five identical fields of interest were chosen in the inferior region per retina (n = 3). During the image quantification, the described areas were marked by a rectangle drawn from either the tip of the outer segments to outer plexiform layer or along the inner segments. Intensity of fluorescence from each rectangle (one area) was measured and the value was plotted as number of pixels/field (Cox Va, green; acrolein, red). The values obtained from each animal were averaged and presented in a histogram.
RNA isolation and cDNA synthesis
Total cellular RNA was extracted from individual retinas and purified using the RNA extraction kit protocol (RNAqueous-Micro kit; Ambion). The purified RNA was quantified on a spectrophotometer (ND-1000; Nanodrop Technologies, Wilmington, DE). The integrity of the samples was assessed using a bioanalyser (2100 Bioanalyzer; Agilent Technologies, Santa Clara, CA). The cDNA was synthesized by reverse transcription using reverse transcriptase or first strand cDNA synthesis kit following the manufacturer’s protocols (Superscript III; Invitrogen, Carlsbad, CA).
Real time quantitative polymerase chain reaction (RT-qPCR)
Quantitative analysis of expression levels for genes Hmox-1 (Mm00516005), C3 (Mm00437838), Fgf-2 (Mm00433287) and was determined by RT-qPCR using Taqman® probes that were combined with the Gene Expression Master-Mix (Applied Biosystems, Foster City, CA). StepOne Plus qPCR machine was used with the StepOne software v2.1 (Applied Biosystems) for analysis. Glyceraldehyde 3-phosphate dehydrogenase (Gapdh) was used as a reference gene. The variabilities were accounted for by performing the Taqman amplification assay in duplicates (individual sample variability) with triplicate biological samples to account for individual animal differences. The fold changes were determined using comparative cycle threshold (Ct; delta-delta ct).
Data were analysed using non-parametric tests, Kruskal-Wallis followed by Mann–Whitney U or parametric ANOVA test with Bonferroni corrections. Results are presented as mean ± Standard Error of the Mean (SEM). P value of <0.05 was considered significant.
Age-related macular degeneration
Association for research in vision and ophthalmology
Cytochrome c oxidase
- Cox Va:
Cytochrome oxidase Va
Complement component 3
Fibroblast growth factor 2
Glyceraldehyde 3-phosphate dehydrogenase
Heme oxygenase – 1
Inner limiting membrane
Outer limiting membrane
Outer nuclear layer
Polyunsaturated fatty acid
Quantitative polymerase chain reaction
Retinopathy of prematurity
Reactive oxygen species
Terminal deoxynucleotidyl transferase dUTP nick end labeling
The authors wish to thank Dr Peter Kozulin for the invaluable technical assistance. The authors are thankful to the animal facility at the Research School of Biology of The Australian National University for the care of the experimental animals. This work was supported by the Australian Research Council Centre of Excellence in Vision Science.
- Whelan HT, Smits RL, Buchman EV, Whelan NT, Turner SG, Margolis DA, Cevenini V, Stinson H, Ignatius R, Martin T, et al: Effect of NASA light-emitting diode irradiation on wound healing. Lasers Surg Med. 2001, 19 (6): 305-314. 10.1089/104454701753342758.View ArticleGoogle Scholar
- Whelan HT: RTO HFM symposium on combat casualty care in ground based tactical situations: trauma technology and emergency medical procedures. vol. 109. DARPA soldier self care: rapid healing of laser eye injuries with light emitting diode technology. 2004, USA: St Pete BeachGoogle Scholar
- Zhang Y, Song S, Fong CC, Tsang CH, Yang Z, Yang M: cDNA microarray analysis of gene expression profiles in human fibroblast cells irradiated with red light. J Invest Dermatol. 2003, 120 (5): 849-857. 10.1046/j.1523-1747.2003.12133.x.View ArticlePubMedGoogle Scholar
- Mester E, Spiry T, Szende B, Tota JG: Effect of laser rays on wound healing. Am J Surg. 1971, 122 (4): 532-535. 10.1016/0002-9610(71)90482-X.View ArticlePubMedGoogle Scholar
- Liang H, Whelan H, Eells J, Wong-Riley M: Near-infrared light via light-emitting diode treated is therapeutic against rotenone- and MPP + −induced neurotoxicity. Neuroscience. 2008, 153 (4): 963-974. 10.1016/j.neuroscience.2008.03.042.PubMed CentralView ArticlePubMedGoogle Scholar
- Oron U, Yaakobi T, Oron A, Mordechovitz D, Shofti R, Hayam G, Dror U, Gepstein L, Wolf T, Haudenschild C, et al: Low-energy laser irradiation reduces formation of scar tissue after myocardial infarction in rats and dogs. Circulation. 2001, 103 (2): 296-301. 10.1161/01.CIR.103.2.296.View ArticlePubMedGoogle Scholar
- Whelan HT, Buchmann EV, Dhokalia A, Kane MP, Whelan NT, Wong-Riley MT, Eells JT, Gould LJ, Hammamieh R, Das R, et al: Effect of NASA light-emitting diode irradiation on molecular changes for wound healing in diabetic mice. J Clin Laser Med Surg. 2003, 21 (2): 67-74. 10.1089/104454703765035484.View ArticlePubMedGoogle Scholar
- Simunovic Z, Ivankovich A, Depolo A: Wound healing of animal and human body sport and traffic accident injuries using low-level laser therapy treatment: a randomized clinical study of seventy-four patients with control group. J Clin Laser Med Surg. 2000, 18 (2): 67-73.PubMedGoogle Scholar
- Muili KA, Gopalakrishnan S, Meyer SL, Eells JT, Lyons JA: Amelioration of experimental autoimmune encephalomyelitis in C57BL/6 mice by photobiomodulation induced by 670 nm light. PLoS One. 2012, 7 (1): 24.View ArticleGoogle Scholar
- Rojas JC, Lee J, John JM, Gonzalez-Lima F: Neuroprotective effects of near-infrared light in an in vivo model of mitochondrial optic neuropathy. J Neurosci. 2008, 28 (50): 13511-13521. 10.1523/JNEUROSCI.3457-08.2008.PubMed CentralView ArticlePubMedGoogle Scholar
- Eells JT, Salomao S, Berezovsky A, Moraes M, Roth JM, Paula H, Sherman J, Lam T, Sadun AA: 670 nm LED treatment of affected carriers of the 11778 Leber’s hereditary optic neuropathy (LHON) mutation in Brazil. Invest Ophthalmol Vis Sci. 2004, 45 (5): 828.Google Scholar
- Eells JT, Henry MM, Summerfelt P, Wong-Riley MT, Buchmann EV, Kane M, Whelan NT, Whelan HT: Therapeutic photobiomodulation for methanol-induced retinal toxicity. Proc Natl Acad Sci USA. 2003, 100 (6): 3439-3444. 10.1073/pnas.0534746100.PubMed CentralView ArticlePubMedGoogle Scholar
- Photobiomodulation for the treatment of retinal injury and retinal degenerative diseases. Edited by: Eells J, DeSmet K, Kirk D, Wong-Riley M, Whelan H, Hoeve J Ver, Nork M, Stone J, Valter K. 2008, New York: SpringerGoogle Scholar
- Qu C, Cao W, Fan Y, Lin Y: Near-infrared light protect the photoreceptor from light-induced damage in rats. Adv Exp Med Biol. 2010, 664: 365-374. 10.1007/978-1-4419-1399-9_42.View ArticlePubMedGoogle Scholar
- Albarracin R, Eells J, Valter K: Photobiomodulation protects the retina from light-induced photoreceptor degeneration. Invest Ophthalmol Vis Sci. 2011, 52: 3582-3592. 10.1167/iovs.10-6664.View ArticlePubMedGoogle Scholar
- Albarracin R, Valter K: 670 nm red light preconditioning supports Müller cell function: evidence from the white light-induced damage model in the rat retina. Photochem Photobiol. 2012, 88 (6): 1418-1427. 10.1111/j.1751-1097.2012.01130.x.View ArticlePubMedGoogle Scholar
- Natoli R, Zhu Y, Valter K, Bisti S, Eells J, Stone J: Gene and noncoding RNA regulation underlying photoreceptor protection: microarray study of dietary antioxidant saffron and photobiomodulation in rat retina. Mol Vis. 2010, 16: 1801-1822.PubMed CentralPubMedGoogle Scholar
- Karu T: Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B. 1999, 49 (1): 1-17. 10.1016/S1011-1344(98)00219-X.View ArticlePubMedGoogle Scholar
- Wong-Riley MTT, Bai X, Buchmann E, Whelan HT: Light-emitting diode treatment reverses the effect of TTX on cytochrome oxidase in neurons. Neuroreport. 2001, 12 (14): 3033-3037. 10.1097/00001756-200110080-00011.View ArticlePubMedGoogle Scholar
- Wong-Riley MT, Liang HL, Eells JT, Chance B, Henry MM, Buchmann E, Kane M, Whelan HT: Photobiomodulation directly benefits primary neurons functionally inactivated by toxins: role of cytochrome c oxidase. J Biol Chem. 2005, 280 (6): 4761-4771.View ArticlePubMedGoogle Scholar
- Barazzone C, Horowitz S, Donati YR, Rodriguez I, Piguet PF: Oxygen toxicity in mouse lung: pathways to cell death. Am J Respir Cell Mol Biol. 1998, 19 (4): 573-581. 10.1165/ajrcmb.19.4.3173.View ArticlePubMedGoogle Scholar
- Dickens F: The toxic effects of oxygen on brain metabolism and on tissue enzymes; tissue enzymes. Biochem J. 1946, 40 (1): 171-187.PubMed CentralView ArticleGoogle Scholar
- Noell WK: Metabolic injuries of the visual cell. Am J Ophthalmol. 1955, 40 (5 Part 2): 60-70.View ArticlePubMedGoogle Scholar
- Bresnick GH: Oxygen-induced visual cell degeneration in the rabbit. Invest Ophthalmol. 1970, 9 (5): 372-387.PubMedGoogle Scholar
- Yamada H, Yamada E, Hackett SF, Ozaki H, Okamoto N, Campochiaro PA: Hyperoxia causes decreased expression of vascular endothelial growth factor and endothelial cell apoptosis in adult retina. J Cell Physiol. 1999, 179 (2): 149-156. 10.1002/(SICI)1097-4652(199905)179:2<149::AID-JCP5>3.0.CO;2-2.View ArticlePubMedGoogle Scholar
- Mervin K, Stone J: Regulation by oxygen of photoreceptor death in the developing and adult C57BL/6J mouse. Exp Eye Res. 2002, 75 (6): 715-722. 10.1006/exer.2002.2064.View ArticlePubMedGoogle Scholar
- Ishikawa K, Yoshida S, Kadota K, Nakamura T, Niiro H, Arakawa S, Yoshida A, Akashi K, Ishibashi T: Gene expression profile of hyperoxic and hypoxic retinas in a mouse model of oxygen-induced retinopathy. Invest Ophthalmol Vis Sci. 2010, 51 (8): 4307-4319. 10.1167/iovs.09-4605.View ArticlePubMedGoogle Scholar
- Karu TI, Kolyakov SF: Exact action spectra for cellular responses relevant to phototherapy. Photomed Laser Surg. 2005, 23 (4): 355-361. 10.1089/pho.2005.23.355.View ArticlePubMedGoogle Scholar
- Zhu Y, Natoli R, Valter K, Stone J: Differential gene expression in mouse retina related to regional differences in vulnerability to hyperoxia. Mol Vis. 2010, 16: 740-755.PubMed CentralPubMedGoogle Scholar
- Natoli R, Provis J, Valter K, Stone J: Expression and role of the early-response gene Oxr1 in the hyperoxia-challenged mouse retina. Invest Ophthalmol Vis Sci. 2008, 49 (10): 4561-4567. 10.1167/iovs.08-1722.View ArticlePubMedGoogle Scholar
- Natoli R, Valter K, Chrysostomou V, Stone J, Provis J: Morphological, functional and gene expression analysis of the hyperoxic mouse retina. Exp Eye Res. 2011, 92 (4): 306-314. 10.1016/j.exer.2010.12.013.View ArticlePubMedGoogle Scholar
- Wellard J, Lee D, Valter K, Stone J: Photoreceptors in the rat retina are specifically vulnerable to both hypoxia and hyperoxia. Vis Neurosci. 2005, 22 (4): 501-507.View ArticlePubMedGoogle Scholar
- Wangsa-Wirawan ND, Linsenmeier RA: Retinal oxygen: fundamental and clinical aspects. Arch Ophthalmol. 2003, 121 (4): 547-557. 10.1001/archopht.121.4.547.View ArticlePubMedGoogle Scholar
- Wassell J, Davies S, Bardsley W, Boulton M: The photoreactivity of the retinal age pigment lipofuscin. J Biol Chem. 1999, 274 (34): 23828-23832. 10.1074/jbc.274.34.23828.View ArticlePubMedGoogle Scholar
- Yu D-Y, Cringle SJ: Retinal degeneration and local oxygen metabolism. Exp Eye Res. 2005, 80 (6): 745-751. 10.1016/j.exer.2005.01.018.View ArticlePubMedGoogle Scholar
- Plafker SM, O'Mealey GB, Szweda LI: Mechanisms for countering oxidative stress and damage in retinal pigment epithelium. Int Rev Cell Mol Biol. 2012, 298: 135-177.PubMed CentralView ArticlePubMedGoogle Scholar
- Uchida K, Kanematsu M, Sakai K, Matsuda T, Hattori N, Mizuno Y, Suzuki D, Miyata T, Noguchi N, Niki E, et al: Protein-bound acrolein: potential markers for oxidative stress. Proc Natl Acad Sci. 1998, 95 (9): 4882-4887. 10.1073/pnas.95.9.4882.PubMed CentralView ArticlePubMedGoogle Scholar
- Natoli R, Provis J, Valter K, Stone J: Gene regulation induced in the C57BL/6J mouse retina by hyperoxia: a temporal microarray study. Mol Vis. 2008, 14: 1983-1994.PubMed CentralPubMedGoogle Scholar
- Li J, Gao X, Qian M, Eaton JW: Mitochondrial metabolism underlies hyperoxic cell damage. Free Radical Biol Med. 2004, 36 (11): 1460-1470. 10.1016/j.freeradbiomed.2004.03.005.View ArticleGoogle Scholar
- Bazan NG: The metabolism of omega-3 polyunsaturated fatty acids in the eye: the possible role of docosahexaenoic acid and docosanoids in retinal physiology and ocular pathology. Prog Clin Biol Res. 1989, 312: 95-112.PubMedGoogle Scholar
- Shen JK, Dong A, Hackett SF, Bell WR, Green WR, Campochiaro PA: Oxidative damage in age-related macular degeneration. Histol Histopathol. 2007, 22 (12): 1301-1308.PubMedGoogle Scholar
- Lin MT, Beal MF: Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature. 2006, 443 (7113): 787-795. 10.1038/nature05292.View ArticlePubMedGoogle Scholar
- Karunadharma PP, Nordgaard CL, Olsen TW, Ferrington DA: Mitochondrial DNA damage as a potential mechanism for age-related macular degeneration. Invest Ophthalmol Vis Sci. 2010, 51 (11): 5470-5479. 10.1167/iovs.10-5429.PubMed CentralView ArticlePubMedGoogle Scholar
- Madsen-Bouterse SA, Kowluru RA: Oxidative stress and diabetic retinopathy: pathophysiological mechanisms and treatment perspectives. Rev Endocr Metab Disord. 2008, 9 (4): 315-327. 10.1007/s11154-008-9090-4.View ArticlePubMedGoogle Scholar
- Izzotti A, Bagnis A, Sacca SC: The role of oxidative stress in glaucoma. Mutat Res. 2006, 612 (2): 105-114. 10.1016/j.mrrev.2005.11.001.View ArticlePubMedGoogle Scholar
- Fitzgerald M, Bartlett CA, Payne SC, Hart NS, Rodger J, Harvey AR, Dunlop SA: Near infrared light reduces oxidative stress and preserves function in CNS tissue vulnerable to secondary degeneration following partial transection of the optic nerve. J Neurotrauma. 2010, 27 (11): 2107-2119. 10.1089/neu.2010.1426.View ArticlePubMedGoogle Scholar
- Peoples C, Shaw VE, Stone J, Jeffery G, Baker GE, Mitrofanis J: Survival of dopaminergic amacrine cells after near-infrared light treatment in MPTP-treated mice. ISRN Neurol. 2012, 850150: 30.Google Scholar
- Kokkinopoulos I, Colman A, Hogg C, Heckenlively J, Jeffery G: Age-related retinal inflammation is reduced by 670 nm light via increased mitochondrial membrane potential. Neurobiol Aging. 2013, 34: 602-609. 10.1016/j.neurobiolaging.2012.04.014.View ArticlePubMedGoogle Scholar
- Anderson DH, Mullins RF, Hageman GS, Johnson LV: A role for local inflammation in the formation of drusen in the aging eye. Am J Ophthalmol. 2002, 134 (3): 411-431. 10.1016/S0002-9394(02)01624-0.View ArticlePubMedGoogle Scholar
- Cashman SM, Desai A, Ramo K, Kumar-Singh R: Expression of complement component 3 (C3) from an adenovirus leads to pathology in the murine retina. Invest Ophthalmol Vis Sci. 2011, 52 (6): 3436-3445. 10.1167/iovs.10-6002.View ArticlePubMedGoogle Scholar
- LaVail MM, Yasumura D, Matthes MT, Lau-Villacorta C, Unoki K, Sung CH, Steinberg RH: Protection of mouse photoreceptors by survival factors in retinal degenerations. Invest Ophthalmol Vis Sci. 1998, 39 (3): 592-602.PubMedGoogle Scholar
- Klein RJ, Zeiss C, Chew EY, Tsai JY, Sackler RS, Haynes C, Henning AK, SanGiovanni JP, Mane SM, Mayne ST, et al: Complement factor H polymorphism in age-related macular degeneration. Science. 2005, 308 (5720): 385-389. 10.1126/science.1109557.PubMed CentralView ArticlePubMedGoogle Scholar
- Wu Z, Lauer TW, Sick A, Hackett SF, Campochiaro PA: Oxidative stress modulates complement factor H expression in retinal pigmented epithelial cells by acetylation of foxo3. J Biol Chem. 2007, 282 (31): 22414-22425. 10.1074/jbc.M702321200.View ArticlePubMedGoogle Scholar
- Hollyfield JG, Bonilha VL, Rayborn ME, Yang X, Shadrach KG, Lu L, Ufret RL, Salomon RG, Perez VL: Oxidative damage-induced inflammation initiates age-related macular degeneration. Nat Med. 2008, 14 (2): 194-198. 10.1038/nm1709.PubMed CentralView ArticlePubMedGoogle Scholar
- Yamada H, Yamada E, Ando A, Esumi N, Bora N, Saikia J, Sung C-H, Zack DJ, Campochiaro PA: Fibroblast growth factor-2 decreases hyperoxia-induced photoreceptor cell death in mice. Am J Pathol. 2001, 159 (3): 1113-1120. 10.1016/S0002-9440(10)61787-7.PubMed CentralView ArticlePubMedGoogle Scholar
- Valter K, Bisti S, Gargini C, Di Loreto S, Maccarone R, Cervetto L, Stone J: Time course of neurotrophic factor upregulation and retinal protection against light-induced damage after optic nerve section. Invest Ophthalmol Vis Sci. 2005, 46 (5): 1748-1754. 10.1167/iovs.04-0657.View ArticlePubMedGoogle Scholar
- LaVail M, Unoki K, Yasumura D, Matthes M, Yancopoulus G, Steinberg R: Multiple growth factors, cytokines, and neurotrophins rescue photoreceptors from the damaging effects of constant light. Proc Natl Acad Sci USA. 1992, 89: 11249-11253. 10.1073/pnas.89.23.11249.PubMed CentralView ArticlePubMedGoogle Scholar
- Dean JB, Mulkey DK, Henderson RA, Potter SJ, Putnam RW: Hyperoxia, reactive oxygen species, and hyperventilation: oxygen sensitivity of brain stem neurons. J Appl Physiol. 2004, 96 (2): 784-791. 10.1152/japplphysiol.00892.2003.View ArticlePubMedGoogle Scholar
- Totan Y, Yagci R, Bardak Y, Ozyurt H, Kendir F, Yilmaz G, Sahin S, Sahin Tig U: Oxidative macromolecular damage in age-related macular degeneration. Curr Eye Res. 2009, 34 (12): 1089-1093. 10.3109/02713680903353772.View ArticlePubMedGoogle Scholar
- Wu WC, Hu DN, Gao HX, Chen M, Wang D, Rosen R, McCormick SA: Subtoxic levels hydrogen peroxide-induced production of interleukin-6 by retinal pigment epithelial cells. Mol Vis. 2010, 16: 1864-1873.PubMed CentralPubMedGoogle Scholar
- Eells JT, Wong-Riley MT, VerHoeve J, Henry M, Buchman EV, Kane MP, Gould LJ, Das R, Jett M, Hodgson BD, et al: Mitochondrial signal transduction in accelerated wound and retinal healing by near-infrared light therapy. Mitochondrion. 2004, 4 (5–6): 559-567.View ArticlePubMedGoogle Scholar
- Karu TI, Pyatibrat LV, Afanasyeva NI: Cellular effects of low power laser therapy can be mediated by nitric oxide. Lasers Surg Med. 2005, 36 (4): 307-314. 10.1002/lsm.20148.View ArticlePubMedGoogle Scholar
- Zhang R, Mio Y, Pratt PF, Lohr N, Warltier DC, Whelan HT, Zhu D, Jacobs ER, Medhora M, Bienengraeber M: Near infrared light protects cardiomyocytes from hypoxia and reoxygenation injury by a nitric oxide dependent mechanism. J Mol Cell Cardiol. 2009, 46 (1): 4-14. 10.1016/j.yjmcc.2008.09.707.PubMed CentralView ArticlePubMedGoogle Scholar
- Cooper CE: Nitric oxide and cytochrome oxidase: substrate, inhibitor or effector?. Trends Biochem Sci. 2002, 27 (1): 33-39. 10.1016/S0968-0004(01)02035-7.View ArticlePubMedGoogle Scholar
- Poyton RO, Ball KA: Therapeutic photobiomodulation: nitric oxide and a novel function of mitochondrial cytochrome c oxidase. Discov Med. 2011, 11 (57): 154-159.PubMedGoogle Scholar
- Liang F-Q, Green L, Wang C, Alssadi R, Godley BF: Melatonin protects human retinal pigment epithelial (RPE) cells against oxidative stress. Exp Eye Res. 2004, 78 (6): 1069-1075. 10.1016/j.exer.2004.02.003.View ArticlePubMedGoogle Scholar
- Lahdenranta J, Pasqualini R, Schlingemann RO, Hagedorn M, Stallcup WB, Bucana CD, Sidman RL, Arap W: An anti-angiogenic state in mice and humans with retinal photoreceptor cell degeneration. Proc Natl Acad Sci. 2001, 98 (18): 10368-10373. 10.1073/pnas.181329198.PubMed CentralView ArticlePubMedGoogle Scholar
- Smith LE: Pathogenesis of retinopathy of prematurity. Semin Neonatol. 2003, 8 (6): 469-473. 10.1016/S1084-2756(03)00119-2.View ArticlePubMedGoogle Scholar
- Maslim J, Valter K, Egensperger R, Hollander H, Stone J: Tissue oxygen during a critical developmental period controls the death and survival of photoreceptors. Invest Ophthalmol Vis Sci. 1997, 38 (9): 1667-1677.PubMedGoogle Scholar
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