Tissue sparing, behavioral recovery, supraspinal axonal sparing/regeneration following sub-acute glial transplantation in a model of spinal cord contusion
© Barbour et al.; licensee BioMed Central Ltd. 2013
Received: 19 February 2013
Accepted: 18 September 2013
Published: 27 September 2013
It has been shown that olfactory ensheathing glia (OEG) and Schwann cell (SCs) transplantation are beneficial as cellular treatments for spinal cord injury (SCI), especially acute and sub-acute time points. In this study, we transplanted DsRED transduced adult OEG and SCs sub-acutely (14 days) following a T10 moderate spinal cord contusion injury in the rat. Behaviour was measured by open field (BBB) and horizontal ladder walking tests to ascertain improvements in locomotor function. Fluorogold staining was injected into the distal spinal cord to determine the extent of supraspinal and propriospinal axonal sparing/regeneration at 4 months post injection time point. The purpose of this study was to investigate if OEG and SCs cells injected sub acutely (14 days after injury) could: (i) improve behavioral outcomes, (ii) induce sparing/regeneration of propriospinal and supraspinal projections, and (iii) reduce tissue loss.
OEG and SCs transplanted rats showed significant increased locomotion when compared to control injury only in the open field tests (BBB). However, the ladder walk test did not show statistically significant differences between treatment and control groups. Fluorogold retrograde tracing showed a statistically significant increase in the number of supraspinal nuclei projecting into the distal spinal cord in both OEG and SCs transplanted rats. These included the raphe, reticular and vestibular systems. Further pairwise multiple comparison tests also showed a statistically significant increase in raphe projecting neurons in OEG transplanted rats when compared to SCs transplanted animals. Immunohistochemistry of spinal cord sections short term (2 weeks) and long term (4 months) showed differences in host glial activity, migration and proteoglycan deposits between the two cell types. Histochemical staining revealed that the volume of tissue remaining at the lesion site had increased in all OEG and SCs treated groups. Significant tissue sparing was observed at both time points following glial SCs transplantation. In addition, OEG transplants showed significantly decreased chondroitin proteoglycan synthesis in the lesion site, suggesting a more CNS tolerant graft.
These results show that transplantation of OEG and SCs in a sub-acute phase can improve anatomical outcomes after a contusion injury to the spinal cord, by increasing the number of spared/regenerated supraspinal fibers, reducing cavitation and enhancing tissue integrity. This provides important information on the time window of glial transplantation for the repair of the spinal cord.
KeywordsTransplantation Tissue sparing Behavioral recovery Spinal cord injury Glia
In models of spinal cord injury (SCI), transplantation of growth-permissive tissues and cells has been performed to provide trophic support for damaged neurons and to transform the inhibitory milieu of the lesion site; this allows axons to grow through the otherwise impenetrable glial scar or cystic cavity. Olfactory ensheathing glia (OEG) facilitate the growth of newly-generated olfactory sensory axons into the olfactory bulb in normal adults [1–4] and after olfactory nerve section [5, 6]. These cells have the potential to facilitate repair after injury, and their potential to enhance tissue repair has been extensively tested in experimental SCI models [7–14]. Schwann cell (SCs) grafts also have the ability to promote axonal regeneration following peripheral and CNS injuries [14–18], and as such were used in this study to compare the outcomes achieved using OEG in sub-acute spinal cord injury model.
Standard methods for isolating adult rat and human SCs have been developed, which result in a homogenous population of cells with stable characteristics [19–22]. However, methods to both isolate the OEG and transplant the OEG vary considerably, as do the experimental results. OEG display considerable morphological plasticity in vitro, changing phenotype within hours after changes in culture conditions [23–26]. Despite this, consistent functional and anatomical improvements have been obtained using olfactory bulb-derived p75-purified OEG from adult inbred rats [8–11, 13, 27]; results with lamina propria-derived OEG are less consistent [28–30].
To initially identify transplanted OEG and SCs, immunohistochemistry using an antibody to low affinity nerve growth factor receptor (p75) was used. However this approach is problematic, because it cannot unequivocally distinguish between transplanted OEG and SCs from endogenous SCs, which may enter the spinal cord via dorsal root entry zones or blood vessels [21, 31–33]. Detection of p75 can sometimes be unreliable, as its ligand-binding domain can be cleaved in vivo and in vitro by endogenous proteases [34, 35]. For this reason, lentiviral ex-vivo pre-labeling [12, 26, 36] of OEG and SCs with DSRED-2 was also used in this study. This allowed the quantification of surviving grafted cells, the analysis of their distribution and influence on endogenous spinal cord cells and axons, and assessment of their impact on matrix deposition and the host repair process.
We hypothesized that transplants of adult OEG or SCs may differ in their ability to promote axonal sparing/regeneration [4, 37] and that a delayed transplant at 14 days post injury would improve anatomical and functional outcomes following a spinal cord contusion. This experimental study is based on numerous years of research into both glial types in CNS injuries, including the spinal cord. This time point was chosen because: (i) it represents a realistic time window deliver this type of cellular therapy in a clinical trial. This time period also gives consideration for time needed to generate and purify sufficient autologous OEGs for transplantation into injured patients, stabilization surgery and an optimal time window for best outcomes , (ii) experimental data from previous animal studies indicate that delayed transplantation may be more beneficial for cell survival, integration and reduced immune mediated rejection [8, 14, 20, 38–41], and (iii) after 15 days in vivo a significant scar forms that may inhibit cell integration and axonal regeneration . In support of this time point, 14 days was the time point chosen for the recent oligodendrocyte precursor and activated macrophage clinical trials. It should also be noted that the term “sparing/regeneration” has been used in relation to the analysis of axonal growth within this manuscript; this is because as described previously in a contusion model study  using fluorogold, we cannot truly distinguish between spared and regenerated axons.
Cell transplantation is associated with improved retention of tissue at the lesion site
Two weeks following cell SCs transplantation (OEG or SCs), there were no significant differences between groups in the amount of total tissue (p=0.27), with 72.4±3.7% >(n=6) of normal in the medium-injected group, 74.6±9.2% (n=6) in the OEG-transplanted group and 85.1±2.9% (n=6) in the SCs-transplanted group. Similarly, there were no significant differences (p=0.25) between groups for intact tissue remaining, which was 29.5±4.6% (n=6) of normal in the medium-injected group, 38.9±7.7% (n=6) in the OEG-transplanted group and 41.6±2.3% (n=6) in the SCs-transplanted group.
It was not until the 4 month time point that total tissue in the transplanted groups was found to be significantly greater than controls (p=0.028). Total tissue was 46.5±6.2% (n=11) of uninjured spinal cord tissue in the medium-injected group, 63.1±3.7% (n=11) in the OEG-transplanted group and 64.1±5.7% (n=11) in the SCs-transplanted group. A significant difference (p=0.028) was also seen between groups at 4 months in the amount of intact tissue remaining, which was 26.0±3.9% (n=11) of normal in the medium-injected group, 39.5±4.1% (n=11) in the OEG-transplanted group, and 35.1±6.4% in the SCs-transplanted group (Figure 1A, B).
Nissl staining/gold chloride staining
Spinal cord sections visualized with Nissl staining showed good preservation of spinal tissue. Cysts that were present were frequently small (see Figure 1D,E and F) and very few in number. As mentioned above, no difference in tissue survival was noted in any of the control or transplanted groups (OEG or SCs). Nissl gold myelin staining at 4 months showed a reduction in tissue loss, particularly the reduction in cyst volume and sparing of white and grey matter within the lesion zone (see Figure 1H,I and J). The presence of round microcysts was noticeable in the spinal cords of SCs treated rats, and were present in 2 week and 4 month spinal cords; neither OEG transplanted rats or control (medium injected) rats showed these cysts.
Transgene expression, cell dispersal and survival of transplanted Schwann cells and OEG
Figures 2C and D show representative spinal cord sagittal sections with OEG and SCs grafts 2 weeks following cell transplantation. Lentiviral-DSRED-2 labeled OEG was detected in all but one of the animals whereas lentiviral-DSRED-2 labeled SCs was detected in all animals. At 2 weeks after transplantation to the injured spinal cord, DSRED-2 fluorescence pixel intensity for surviving transplanted OEG was 70±29 at the rostral aspects of the transplant/lesion zone, versus 91±37 for SCs, at the center of the lesion 86±35 for OEG and 100±41 for SCs. Lastly the DSRED-2 fluorescence pixel intensity at the caudal edge of the transplant/lesion was 83±34 for OEG and 79±32 for SCs. In rats at 2 weeks (n=6 per group) analyzed, detectable transplanted OEG had in some cases dispersed up to 5 mm from the center of the injection site, the furthest distance measured. In contrast, DSRED-2 positive transplanted SCs were highly localized to the site of injection, dispersing no more than 0.6 mm from the injection site. At 2 weeks post transplantation the surviving OEG were dispersed throughout the lesion site and adjacent spinal cord tissue, and were aligned along the rostro-caudal axis of the spinal cord. SCs were densely packed, with an alignment that was highly regular within the graft area but did not disperse into the intact host CNS tissue surrounding the lesion site.
Four months after OEG transplantation, DSRED-2 positive OEG were detected in 7 out of 11 animals (64%) whereas DSRED-2 positive SCs were detected in 11 out of 11 animals (100%). Measurements of DSRED-2 fluorescence pixel intensity showed OEG at the rostral part of the lesion at 22±9 and SCs 34±14, at the center of the lesion the values were 21±9 for OEG and 29±12 for SCs. DSRED-2 pixel fluorescence was found to be 19±8 for OEG at the caudal part of the lesion and 28±12 for SCs. As estimated from the DSRED-2 fluorescence, the levels of OEG present within the spinal cord had decreased throughout the rostral-caudal axis of the lesion by 74% at 4 months from the value seen at 2 weeks post transplantation. At the same time DSRED-2 fluorescence pixel values for SCs reduced from 2 weeks and 4 months, however fluorescence levels were significantly higher than OEG levels (p<0.05) (see Additional file 1: Table S1).
Scar formation, CS56 synthesis and gliosis
Integration of transplanted cells with host astrocytes was frequent for the OEG transplant groups but infrequent for the SCs transplant animals. CS56 proteoglycan staining was analyzed across the OEG, SCs and medium groups in the 4 month post transplant spinal cords (n=11). Immunofluorescence staining for CS56 in the SCs transplanted group (Figure 3E) was significantly greater than medium injected control sections (p<0.001) (Figure 3F) and the CS56 immunofluorescence was greater in the SCs group compared to the OEG transplanted group (p<0.01) (Figure 3F). CS56 immunofluorescence in the OEG group was not significantly different from the levels seen in the medium injected controls.
Extracellular matrix deposition within the lesion site
The profile of immunoreactivity for collagen IV (Figure 4C and L-S) was similar to that observed for laminin-1. Structures resembling blood vessels were visible around the site of the spinal cord lesion in the OEG transplanted group (Figure 4L). While the graft site of the OEG transplanted group contained more collagen IV immunofluorescence than that of the other groups (Figure 4L and P vs. C), measurement using pixel intensity revealed the collagen IV immunofluorescence for each group to be statistically similar (p=0.10). As with laminin-1, at higher magnification, collagen IV could be seen in high quantities in regions of the SCs grafts. However in the OEG transplanted group high amounts of collagen IV appeared to originate from DSRED-2 negative cells in the vicinity of the DSRED-2 positive cells (Figure 4O,S), which may represent either endogenous SCs invading from the periphery or host endothelial cells.
Axonal profiles inside lesion site
Evidence for infiltration of endogenous Schwann cells into the spinal cord
Numbers of retrogradely labeled descending axons increased by glial transplantation
Retrograde labeling with Fluorogold was used to quantify extension of spared/regenerated descending supraspinal axons reaching 8 mm beyond the most caudal edge of the contusion site. Mean±standard errors of numbers of descending Fluorogold-labeled cells are presented. There were statistically significant differences (p<0.05) between the total brain counts of the OEG transplanted group (3019±216) and also the SCs transplanted group (2767±411) when compared to the control injury only group (1204±331).
Retrogradely Fluorogold-Labeled Neurons in Control, OEG Transplanted and SC Transplanted groups
Contusion only *
Medium only #
Schwann and OEG cell grafts increase the number of spared/regenerated propriospinal neurons
Functional recovery: open field behavioral testing (BBB) and Ladder walk testing
Scoring scale developed for the ladder rung walking test, modified from (Metz and Whishaw, [])
Deep slip then jump
Deep slip, unsuccessful or no paw replacement
Deep slip, paw replacement, slight slip
Step, unsuccessful paw replacement
Deep slip, successful paw replacement
Step, paw replacement, slight slip
Step, good paw replacement
Slight slip, short stride
Slight slip, regular stride
Plantar paw placement, short stride
Plantar paw placement, regular stride
We have shown the capacity of purified, primary OEG and SCs to promote behavioral recovery, increase numbers of supraspinal axons, and increase tissue sparing when transplanted two weeks after a moderate spinal cord contusion injury. A lentiviral vector encoding DSRED-2 was used to transduce OEG and SCs – in this study it was shown to be an effective and stable transgene to label both cell types up to 4 months in vivo following transplantation. Viable transplanted OEG and SCs were found in similar numbers at 2 weeks, but after 4 months the OEG numbers were less than the number of SCs remaining. Histochemical tissue analysis showed a significant reduction in tissue loss in both the OEG and SCs groups 4 months post-transplantation. Further, dorsal white matter tracts appeared to be better protected in the transplant groups than in non-cellular transplanted controls (medium). Analysis of DSRED-2 labeled glia in the spinal cords showed a migration/dispersal difference between the two cell types. OEG were more evenly distributed in a rostro-caudal direction, and greater integration of OEG with host astrocytes was evident when compared to the tight interwoven grafts in the SCs transplanted rats. OEG transplanted rats displayed significantly lower levels of CS56 immunofluorescence within and around the lesion/transplant site when compared to the SCs group. Matrix deposition was similar between OEG and SCs groups, but laminin and collagen IV immunofluorescence showed a closer co-existence of these matrix molecules to SCs than to OEG. Axonal staining using neurofilament antibodies showed tighter bundles of axons within the SCs transplant zone than in OEG zones. Open field behavioral testing (BBB) showed significant differences between the transplanted groups and the controls up to 4 months after transplantation. BBB scores were consistently higher in the OEG group compared to control injury, medium and SCs injected rats. Additional behavioral testing using the ladder walk however, did not show any significant differences between experimental and control groups. Fluorogold retrograde tracing 8 mm distal to the injury/transplantation site revealed significant differences between control groups and OEG and SCs transplanted groups in supraspinal projecting axon populations. This provides strong evidence that a sub-acute transplant of SCs or OEG provide an environment in which promotion of sparing/regeneration of supraspinal axonal projections to the spinal cord are increased.
SCs and OEG differ in their interaction with host spinal cord cells and matrix production
Evidence of statistically significant tissue-protective effects of OEG was observed 4 months after transplantation. At 2 weeks post-transplantation, tissue loss was still occurring. The tissue-protective effects of transplanted OEG or SCs arise from the secretion of factors from the cells such as BDNF, NGF, GDNF and NT4/5 [13, 46–48] or matrix (laminin-1, collagen IV) and adhesion molecule (N-Cadherin) secretion [49–51]. These mechanisms of protection combined with tissue-rebuilding or new cell birth , are potentially acting together to improve anatomical functional outcomes.
Proliferation of donor OEG seems unlikely, as the labeling method employed in this study would result in all daughter cells also being labeled; the number of labeled cells at 4 months in the lesion site were fewer than when counted at 2 weeks. The fewer numbers of detectable OEG involves movement away from the site of injury (measurement area), or death (via apoptotic or anoikis mechanisms) [42, 44]. In a small number of animals, OEG were observed several millimeters from the injection site which is similar to earlier studies which reported OEG migration at distances of 9 mm from the injection site [9, 53, 54]. In contrast to the OEG, SCs were not observed very far from the immediate lesion area, dispersing only up to a distance of 1.5 mm. A similar lack of dispersal has been reported out of peripheral nerve grafts inserted into the adult CNS [55, 56]. This is likely due to interactions with host astrocytes and other endogenous non-neuronal cells .
The observed difference in the amount of intact tissue between the OEG and SCs transplanted groups indicates chemotropic effects of the OEG , similar to the chemotropic effects exerted by glia during development  that result in increased infiltration of various cells into the lesion site. OEG have the capacity to stimulate a different inflammatory response by the secretion of chemokines such as CX3CL1 , which is a known ligand for the receptor CX3CR1 found on microglia and dendritic cells [60, 61] and affects the amount of tissue preservation/removal. OEG potentially are be more susceptible to death and subsequent phagocytosis by macrophages than SCs. OEG are known to secrete different factors and have unique antigenic profiles other than the ones mentioned above which make them more susceptible to immune attack .
OEG and SCs transplants have the properties to directly influence proliferation or differentiation of spinal cord progenitor cells/stem cells [52, 63]. Glial transplants have the potential to differentiate these stem cells, like fibroblasts , into neurons or myelinating glia [64–67]. In our study we did not examine the direct OEG or SCs myelination potential or host myelination within the lesion site. Both cell types have the capacity to myelinate but differ in their effectiveness and mechanisms involved [75, Plant et al., -unpublished observations]. The proliferation of endogenous cells does occur in SCI . These cells can include progenitor cells, endothelial and pericytes stimulated by factors such as epidermal growth factor and/or fibroblast growth factor-2 [68–70]. Proliferation seems highly plausible especially when OEG and SCs are known to produce similar levels of FGF-2 . Two recent findings have indicated new cells play a role in SCI. New evidence from Goritz and colleagues  has indicated that pericytes play a role in the spinal cord injury response, whereby these cells actively migrate to sites of injury. OEG and SCs in our model are likely to have altered the number of these cells in the lesion site, thus providing a reparative outcome. In addition, evidence by Decimo and colleagues  found new adult stem cell /precursor cells that are resident in the meninges react to SCI and populate the injury site, thus providing a target for glial cell differentiation or activation.
In this study we provide evidence that following spinal cord contusion injury, endogenous repair processes including SCs migration were ongoing. Proliferation of ependymal cells in the walls of the central canal and from adjacent intact parts of spinal cord [45, 52, 65, 74, 75] is one route. Ependymal cells are known to migrate and populate the lesion site along with new blood vessels, astrocytes and fibroblasts. This seems likely to have contributed to the cellular trabeculae and scar tissue seen within our lesion cysts [45, 75, 76]. Host SCs from the dorsal roots or blood vessels also infiltrated the lesion site in our model, based on large numbers of p75-positive/DSRED-2 negative cells in all groups, as has previously been observed in the rat [14, 45, 55, 77, 78] and human  with the majority from the dorsal roots .
In a previous study from our laboratory , we indicated that molecules (including neurotrophins) released by Schwann cells promote the reactivity of astrocytes. Transplanted SCs stimulate astrocytes to be highly GFAP positive and surrounded by CS56 staining. This forms a dense glial scar around the lesion and prevents the entry of axons, and/or other host non-neuronal cells . In this study we demonstrate in sub-acute 14 day glial transplants, OEG were associated with significantly lower CS56 expression and slightly higher collagen IV expression in the lesion and graft site compared to SCs. Since OEG are proficient in interacting with astrocytes in vitro[49, 80], their release of extracellular matrix and cell adhesion molecules and their modulation of these factors may also account for the observed axonal infiltration into the glial scar . Molecules such as Fibulin-3 and its ability to bind TIMP-3 may also play a part in the different interaction with host glia . OEG isolated from the olfactory bulb show high levels of this protein when compared to SCs . One recent report has also suggested an important role of MMP’s in cell migration and modulation of the ECM . While both OEG and SCs appeared to be associated with a greater quantity of extracellular matrix molecules in the lesion site compared to the effect of medium injection alone, their profiles differed. The laminin was present in a complementary and non-overlapping pattern to DSRED-2 labeled SCs and OEG. SCs appeared to produce basement membrane, while OEG are capable of producing these molecules but not in the quantity seen in SCs grafted animals; rather, they may have induced other cells to produce extracellular matrix such as astrocytes or endothelial cells. This is not surprising given the role the OEG play in the olfactory system, where OEG form channels which stay present even after axonal degeneration in the olfactory bulb for axons to grow through and out to innervate areas of the olfactory bulb [83, 84]. One mechanism for OEG reparative outcomes in the CNS is their close “cross-talking” with fibroblasts/meningeal cells [see [48, 81] and ability to interact with these cells [9, 85]. OEGs have a very close relationship with fibroblasts in the olfactory system, as do SCs in the peripheral nerve, but their role seems very different. SCs are potentiated to myelinate axons by fibroblasts and their associated matrix , whereas OEG interact in an axonal replacement/regenerating system without myelination . In addition they have previously been shown play a role in fascicle-forming arrangements without fibroblasts [88–90].
Olfactory glia and Schwann cells increase axonal sparing/regeneration in sub acute treated SCI
Our results show that transplantation of OEG or SCs at 2 weeks after contusion facilitated significant axonal sparing/regeneration comparable to those seen in transplants carried out 7 days after a contusion injury . Significant increases in regenerated propriospinal fibers were also present in both graft groups, but numbers in SCs treated animals were significantly higher than after OEG transplantation. This indicates a more potent, local secretion of factors by SCs compared to OEG, but as yet no study has extensively analyzed these differences. In this study, an OEG or SCs transplantation following a contusion injury was associated with an increased amount of intact tissue at the lesion site. Immunohistochemistry for neurofilament-positive axons showed strongly immunoreactive profiles at the lesion site for both OEG and SCs transplanted groups after a 14 day delay of transplantation. Neurofilament positive axons in the SCs group were less linearly organized than those seen in the OEG group. This could be explained by the interaction with host astrocytes not allowing for the even distribution of the cells and the formation of a clear cellular boundary .
Retrograde tracing using Fluorogold revealed statistically significant increased numbers for supraspinal tracts after OEG or SCs transplantation. Supraspinal spinal tracts such as reticular, vestibular and hypothalamic were seen to respond similarly in both transplant groups. It should be noted that these supraspinal fibers were measured from 8 mm distal to the injury site, and so possible differences in fiber numbers between the transplanted cell groups at the lesion site itself cannot be discounted. Unlike the other spinal tracts, the raphe tract responded in a far more robust fashion in the OEG group. This can be via increased responsiveness to BDNF from the OEG directly or by the interaction with activated microglia which are known to secrete BDNF after ATP activation through the P2X4 receptor . OEG differ to SCs in their levels of fractalkine secretion, and this can activate microglia to become more active and secrete different neurotrophic profiles to that initiated by SCs that do not secrete this protein at the same level. An important consideration, that is difficult to assess at the time of cell delivery, is the stage of cellular differentiation of transplanted glia after a period of time in vivo. SCs will differentiate into myelin forming cells quite quickly thus reducing their regenerative/autocrine secretory products [93–95] whereas OEG stay in an undifferentiated [p75-positive) non-myelin forming state for a longer periods (Plant – unpublished observations) or may not myelinate at all .
Functional recovery is improved after sub acute transplantation of OEG and SCs
Significant behavioral differences were observed between the control injury and medium injected rats, and both OEG and SCs grafted groups. Improvements, which are normally observed in studies similar to that undertaken here, may result from compensatory effects occurring within reflex-based segmental cord systems . OEG transplantation into a completely transected spinal cord has elicited improved hindlimb function [9, 10, 97] as seen here. One possibility that could increase locomotion is increased noradrenergic and serotonergic varicosities apposing the motor associated cholinergic neurons . OEG treated rats showed significant numbers of raphe projecting axons reaching at least 8 mm distal from the injury site and these could stimulate such a behavioral response. This however does not explain the SCs grafted rats which may use an alternate but effective pathway of locomotion. SCs induced recovery could be partially explained by a graft relay system developed through regeneration of propriospinal axons regeneration as seen in our results here. Only small numbers of spared fibers (<5%) are required to facilitate compensatory return of function via segmental circuits . Phasic behavioral recovery in the SCs group, in particular 49 days until the end of the experiment, may be due to phasic secretion of neurotrophins which may not be delivered at the same level via the transplantation of OEG.
Glial cells transplanted at 14 days post injury may experience a less accommodating lesion site than when grafted 1 week after injury, which may explain a reduced glial survival in particular for OEG than seen in earlier transplant studies. At 14 days post SCI proteoglycan deposition at the lesion site is present and highly inhibitory [100, 101] but was shown to be reduced in the OEG transplanted groups providing a positive milieu for the sparing/regeneration of supraspinal axons (serotonergic positive fibers). This evidence is strongly backed by our use of fluorogold tracing, but was not supported by immunohistochemistry for 5-HT  (which labels serotonergic axons innervating the spinal cord). Supplementation of glial transplants with neurotrophins may improve cell survivability  and in combination with removal of the glial scar by the use of chondroitinase could potentially improve axonal regeneration in this 14 day delayed glial transplant model [102, 103]. Clearly the delay between injury and treatment can have a strong bearing on the experimental outcome but also the success of the glial type to improve tissue and behavioral outcomes, modify the lesion site and improve sparing/regeneration of axonal populations as shown here. From the present study it would appear that a 14 day delay in glial transplantation is feasible and beneficial both anatomically and behaviorally after a contusive spinal cord injury but the mechanisms of repair of the two glial types are different. We have examined two time points (2 weeks and 4 months) using multiple behavioral and anatomical analysis, but results can only give an indication of efficacy. Further physiological, behavioral and anatomical correlative studies using these glial cells at 14 days post injury time point would provide valuable evidence of cell potential and reproducibility for future CNS repair applications.
Adult (180-200 g) female Fischer 344 rats (Animal Resource Centre, Murdoch, Western Australia) were housed in groups of three under controlled conditions of temperature and humidity, with free access to food and water. All procedures were approved by the Animal Ethics Committee of The University of Western Australia (approval number 99/008/E42). Fourteen days after injury, previously described injection procedures  were used to transplant OEG (n=6 short term 2 weeks, and n=11 long term 4 months) or SCs (n=6 short term 2 weeks and n=11 long term 4 months) into the lesion site controls received an injection of cell culture medium (n=6 short term 2 weeks and n=11 long term 4 months) or injury alone (n=6 short term 2 weeks and n=11 long term 4 months). Six additional rats were perfused 2 weeks after contusion without a transplant, to examine the lesion milieu into which transplanted cells would have been introduced. Total rats used n=74. All rats receiving a transplant and/or medium were perfused at 2 weeks or 4 months after transplantation. The numbers of animals were final numbers analyzed. In the long term groups 3 animals died and in the short term groups 6 rats.
Olfactory ensheathing glia and Schwann cell culture and purification
OEG were prepared from the olfactory bulbs of adult female Fischer 344 rats [8, 13, 33]. In brief, the bulbs were extirpated and the olfactory nerve layer dissected. Tissue was mechanically dissociated and treated with trypsin 0.5% w/v (Worthington Biochemical Corporation, Lakewood, NJ) in Hank’s Balanced Saline Solution (Sigma) for 60 minutes at 37°C in a CO2-free incubator. Tissue was pelleted by centrifugation at 1500 rpm for 5 minutes, resuspended in DF10S medium (1:1 DMEM/F-12 -Sigma), 10% (v/v) fetal calf serum - Hunter Antisera) supplemented with bovine pituitary extract (20 μg/mL Gibco) and forskolin (2 μM Sigma), then plated on poly-l-lysine-coated culture dishes (Corning, Acton, MA) for one week. OEG were purified by immunoaffinity to an antibody to the p75 receptor (192-IgG) [13, 33].
SCs were isolated from adult female Fischer 344 rats [19, 33]. Briefly, the sciatic nerves were dissected, placed in 35 mm uncoated tissue culture dishes in D10S medium consisting of DMEM (Sigma St Louis, MO) with 10% fetal calf serum (Hunter Antisera Jesmond, NSW, Australia) and cut into 1-2 mm3 pieces. Each week, nerve segments were transferred to a new culture dish, leaving behind fibroblasts that had migrated out of the explants. After three weeks of fibroblast depletion, explants were enzymatically and mechanically dissociated and transferred to new dishes for culture in the presence of bovine pituitary extract (20 μg/mL Gibco, Carlsbad, CA) and forskolin (2 μM Sigma) .
Lentiviral labelling of glia
Lentiviral vectors encoding the gene for DSRED-2 were made according to methods described by . Cultured OEG or SCs were labeled by adding the vector (using a multiplicity of infection of 100  to the culture medium for 16 hours, followed by 2 washes in culture medium and fresh media for growth.
Spinal cord injury and post-operative care
Anesthesia was induced with 5% (v/v) halothane (in 60:40 O2:N2O) then maintained at 2-3%. The skin and muscle layers were cut rostro-caudally to expose the vertebral column, and a T10 laminectomy performed  Using an New York University (NYU) impactor, a moderate contusion injury was induced by dropping a 10 g weight from a 12.5 mm height [99, 105] Gel-Foam (Pharmacia & Upjohn, Kalamazoo, MI) was used to stop bleeding in some animals. Animals were partially suspended from clamps to the dorsal processes of T8 and T11/12, to circumvent diaphragm-induced dorso-ventral movement of the spinal column. Immediately after the injury, the animal was removed from the clamps and the muscles sutured in layers with Vicryl (Johnson & Johnson, New Brunswick, NJ). Skin was closed with Michel suture clips (Fine Science Tools Inc., North Vancouver, BC, Canada). Immediately after surgery, each animal was injected with penicillin (Benacillin intramuscular), analgesic (Temgesic buprenorphine sub-cutaneous 0.1 mg/kg) and 2-4 mL 0.9% saline (sub-cutaneous). Animal cages were placed partially onto a heating pad for 24 hours, to assist with maintenance of body temperature. Antibiotics were continued on alternate days, with analgesic twice daily and saline once daily for one week. Manual bladder expression was performed twice daily for two to three weeks, by which time all animals had recovered bladder tone.
Cells were transplanted after no more than 2 weeks in vitro. Fourteen days post-contusion, adult female rats were anesthetized and the spinal cord exposed as described above. Either 5 μL of a cell suspension containing 5 × 105 OEG or SCs in culture medium, or medium alone, (1:1 DMEM/F-12 Sigma) was injected into the center of the lesion over a period of 3 minutes using a 5 μL syringe (Hamilton, Reno, NV) mounted in a stereotaxic frame. The needle was withdrawn 3 minutes later to encourage cell integration into the injection site. Wounds were sutured and post-operative care was administered as described above.
All testing of the BBB and ladder walk were carried out by observers blinded to the treatments. BBB testing  was performed on each animal on the day following contusion, to confirm the successful lesion (a score of 0). The BBB test was repeated on day 3, day 7 and day 14 after contusion thereafter every week after cell transplantation. 3 weeks post-injury, animals were consistently scoring BBB results of 10–11. A ladder rung walking test modified from  was introduced 1 week after injury and measured every week for 4 months. The ladder rung walking test provided 6 points (Table 2) for scoring, thus allowing a greater level of sensitivity than the two BBB points by which they were separated.
The ladder rung walking test  apparatus was 1.5 m in length and consisted of two clear Perspex sidewalls and a floor with removable metal rungs spaced 1 cm apart. Prior to surgery, rats were trained to walk along the ladder to reach their home cage. From 1 week after injury, trials were performed weekly and video recorded. The animals had 3 runs per day when tested. The video camera was positioned to capture the central portion of the ladder, approximately 30 cm in length, allowing analysis of approximately 8 steps of each hind limb. Each hind limb step in each trial was later scored (Table 2) the scores were averaged to give a single value, per rat, per trial.
Ten days prior to perfusion (4 months post-transplantation of cells), 11 rats from each of the long term (4 month) groups were anesthetized as above and subjected to a T12 laminectomy. While held in a stereotaxic frame, Fluorogold (1 μL Fluorochrome Inc., Denver, CO) was injected into the spinal cord at 4 points (0.25 μL each), 2 segments caudal (8 mm) to the contusion site. All injections were performed by the same individual, to maximize comparability between animals this individual was also blinded as to which groups had previously been injected with OEG, SCs or control cells. Muscles were sutured in layers and the skin closed with Michel suture clips (Fine Science Tools Inc., North Vancouver, BC, Canada). Animals were treated with the post-operative care regime detailed above.
At 2 weeks or 4 months after Schwann cell or OEG transplantation, rats were terminally anesthetized with Nembutal (intraperitoneal sodium pentobarbital Merial Australia, Parramatta, NSW, Australia) and perfused intracardially with approximately 150 mL of heparinized phosphate-buffered saline (Heparin 1 U/mL David Bull Laboratories, Melbourne, VIC, Australia) followed by approximately 150 mL 4% buffered paraformaldehyde solution. The spinal cord and brain were immediately removed and post-fixed in buffered paraformaldehyde overnight at 4°C. The following day, paraformaldehyde was replaced with 0.1 M phosphate buffer. Prior to tissue sectioning, each spinal cord or brain was placed into sucrose (30% w/v in phosphate buffer) overnight and embedded in gelatin (10% w/v in phosphate buffer Difco, Franklin Lakes, NJ). The gelatin block was fixed by immersion in buffered paraformaldehyde for 2 hours and placed into buffered sucrose solution overnight. Tissue was sectioned (spinal cord at 40 μm brain at 50 μm) using a freezing sledge microtome and placed into phosphate buffer containing 0.05% w/v sodium azide.
Tissue analysis - Quantification
Numbers of animals per group were 8–12 and based on: (i) a prior power analysis study to ascertain experimental numbers in animal models of spinal cord injuries, in order to appropriately assess statistical differences, and (ii) previous publications from the laboratory. All rats were tested with BBB, gridwalk, fluorogold traced, immunohistochemistry, and tissue analysis. These were randomly allocated to the groups without the knowledge of the surgeon or behaviorist. Animals were taken from the naïve cages and re-housed into post-surgery cages and numbered. This number was then combined by an independent person to identify the treatment. At every level the person was blinded to the treatment.
The perfusions were performed on the same day for all groups with the same fixative batch. The immunohistochemistry was also carried out between each of the groups reducing differences in staining between days and groups. The analysis was performed by a trained viewer who was blinded to the groups.
Tissue volume estimation
One in six sections from each spinal cord was mounted onto gelatin-coated microscope slides and stained with gold chloride solution for myelin followed by cresyl violet for Nissl substance . Slides were dehydrated through an ethanol/toluene series and mounted in DPX (Chem Supply, Gillman, Australia). Sections were visualized using a Leica DM RBE microscope and digitally photographed. Image Pro Plus (Media Cybernetics, Silver Spring, MD) was used to measure the tissue area of a 4.5 mm section of spinal cord with the lesion center at its midpoint.
Tissue cavitation was measured in all animals to gain an estimate of the effect of the transplanted cells upon the preservation of tissue at the lesion site the volume of tissue remaining at the injury site was measured in a similar manner to that described previously [8, 14, 108] The combined area of any cysts was calculated and subtracted from the total tissue area of the section, to give a value for the total tissue remaining. Areas showing signs of degeneration, such as microcysts and loss of neuronal profiles, were subtracted from the values for total tissue remaining to obtain a measure of intact tissue. The values for the centre four sections of each spinal cord were averaged to give a single semi-quantitative value of remaining tissue volume value for each rat. Values from four equivalent sections of an uninjured spinal cord were used to compute the tissue remaining as a proportion of uninjured spinal cord.
Series of one in six tissue sections (in 2 week or 4 month time points) were immunostained free-floating with mouse monoclonal or rabbit polyclonal antibodies. Sections were incubated overnight at 4°C in primary antibody diluted in phosphate buffer containing 10% normal goat serum and 0.2% (v/v) Triton X-100. Mouse monoclonal antibodies used were: i) anti-chondroitin sulfate proteoglycans (CS-56 1:50 Sigma) to identify proteoglycan deposition at the lesion site, ii) low affinity nerve growth factor receptor p75 (supernatant 192-IgG) to stain for OEG and Schwann cells (both transplanted and endogenous) and iii) anti medium/high neurofilaments RT-97 (supernatant Developmental Hybridoma Bank) to stain for spared and regenerated axons. Rabbit polyclonal antibodies were used for: i) p75 (1:200 Promega, Madison, WI), Glial fibrillary acidic protein (GFAP) (1:500 DakoCytomation, Denmark) to stain for astrocytes, unmyelinated Schwann cells and olfactory glia, ii) anti-collagen IV (1:200 Rockland Inc., Gilbertsville, PA) to ascertain the distribution of basal lamina and blood vessels and iii) anti-laminin-1 (1:200 Sigma) for basal lamina and blood vessels.
The following day, sections were washed three times with phosphate buffer and incubated with secondary antibody (diluted in the same solution as for primary antibodies) for 30 minutes at room temperature. Sections were washed three times with phosphate buffer, mounted onto gelatin-coated glass microscope slides, air dried and then coverslipped in a glycerol-based mounting medium.
Semi-quantitative analysis of proteoglycan, matrix and cell survival in vivo
One in 6 series of sections at 4 months post transplantation (and controls) were analyzed. Low magnification photomicrographs (10× objective) were taken using an IX70 Olympus microscope. Pixel fluorescence of immunostaining for CS-56, laminin and collagen IV was measured at rostral, middle and caudal locations of the contusion/injury sites and the average pixel fluorescence minus background readings of the three areas compared between medium control and OEG or SCs groups [37, 57]. In addition, one in 4 series of sections were analyzed for the presence of DSRED-2 labeled cells in the 2 week and 4 month survival groups. Pixel fluorescence was then compared between the 2 survival points to estimate survival of OEG and SCs at 4 months expressed as a percentage of those present at 2 weeks.
Fluorogold photography and counts
A 1 in 6 series of brain sections were mounted onto gelatin-coated glass microscope slides, air-dried and mounted in a glycerol-based mounting medium. Sections were photographed with a Hitachi HV-C20M digital camera as a large tiled image using a fluorescence microscope (Leica DM RBE) with a motorized stage controlled with Image Pro Plus (5.1) software (Media Cybernetics). The optical dissector method was used to photograph and count fluorogold-labeled neurons, in order to avoid inaccuracies due to double counting  Photographs were taken in the centre of the z-axis of each section. Those cell bodies in focus were identified, then tagged and counted using Image Pro Plus. Seven areas were analyzed: the reticular formation, vestibular nucleus, trigeminal and dorsal column nuclei, raphe nucleus, red nucleus, hypothalamus and motor cortex.
To quantify sparing/regeneration of descending propriospinal neurons, the labeled cell bodies were counted using an identical method in the spinal cord region rostral to the lesion site. Six spinal cord sections (1 cm in length) from each animal were analyzed.
The methods of statistical analysis were chosen that reflected the animal numbers used in this study. For Fluorogold labeling of supraspinal projecting nuclei in the brain, experimental groups were compared using a one-way analysis of variance (ANOVA) followed by the Dunnetts methods of multiple comparisons versus a single control group (injury only) and also the Tukey test for multiple comparison procedures between all experimental and control groups. Fluorogold analysis of propriospinal projections into the distal spinal cord were analyzed using a one way analysis of variance (ANOVA). This was also the case for the tissue sparing and proteoglycan and cell survival analysis. In the analysis of the BBB scores, groups were compared using the non parametric Kruskal Wallace ANOVA on ranks. This was followed by Dunnetts method of multiple comparisons versus a single control group.
Olfactory ensheathing glia
Basso, Beattie and Bresnahan
One way analysis of variance
Spinal cord injury
Green fluorescent protein
Glial fibrillary acidic protein
Brain derived neurotrophic factor
Nerve growth factor
Glial derived neurotrophic factor
Dulbecco’s modified eagle’s medium
New York University.
We wish to express thanks to Seok Voon Lee for technical contributions. We sincerely thank William Hendriks and Joost Verhaagen for their help with lentiviral vector design and production. GWP’s laboratory was supported by the National Health and Medical Research Council (RD Wright Fellowship N°. 303265), Eileen Bond, Australian Rotary Health Research Fund, Spinal Cure Australia. Stanford University, James Doty Neurosurgery Fund, and Hearst Neuroscience Fund.
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