SB 206553, a putative 5-HT2C inverse agonist, attenuates methamphetamine-seeking in rats
© Graves and Napier; licensee BioMed Central Ltd. 2012
Received: 10 January 2012
Accepted: 1 June 2012
Published: 14 June 2012
Methamphetamine (meth) dependence presents a substantial socioeconomic burden. Despite the need, there is no FDA-approved pharmacotherapy for psychostimulant dependence. We consider 5-HT2C receptors as viable therapeutic targets. We recently revealed that the atypical antidepressant, mirtazapine, attenuates meth-seeking in a rodent model of human substance abuse. Mirtazapine historically has been considered to be an antagonist at 5-HT2C receptors, but more recently shown to exhibit inverse agonism at constitutively active 5-HT2C receptors. To help distinguish the roles for antagonism vs. inverse agonism, here we explored the ability of a more selective 5-HT2C inverse agonist, SB 206553 to attenuate meth-seeking behavior, and compared its effects to those obtained with 5-HT2C antagonists, SDZ Ser 082 and SB 242084. To do so, rats were trained to self-administer meth and tested for seeking-like behavior in cue reactivity sessions consisting of contingently presenting meth-associated cues without meth reinforcement. We also explored motor function to determine the influence of SB 206553 and SDZ Ser 082 on motor activity in the presence and absence of meth.
Like mirtazapine, pretreatment with SB 206553 (1.0, 5.0, and 10.0 mg/kg), attenuated meth-seeking. In contrast, the antagonists, SDZ Ser 082 (0.1, 0.3, and 1.0 mg/kg) and SB 242084 (3.0 mg/kg) had no effect on cue reactivity (CR). SB 242084 (3.0 mg/kg) failed to attenuate the effects of 5.0 and 10 mg/kg SB 206553 on CR. Motor function was largely unaltered by the 5-HT2C ligands; however, SB 206553, at the highest dose tested (10.0 mg/kg), attenuated meth-induced rearing behavior.
The lack of effect by 5-HT2C antagonists suggests that meth-seeking and meth-evoked motor activity are independent of endogenous 5-HT acting at 5-HT2C receptors. While SB 206553 dramatically impacted meth-evoked behaviors it is unclear whether the observed effects were 5-HT2C receptor mediated. Thus, SB 206553 deserves further attention in the study of psychostimulant abuse disorders.
KeywordsMethamphetamine Serotonin Self-administration Motor Inverse agonist Seeking
Psychostimulants such as cocaine and methamphetamine (meth) dramatically enhance transmission of monoamines, including serotonin (5-HT). It is clear that the 5-HT2C receptor subtype is involved in stimulant-mediated behaviors [1, 2]. For example, 5-HT2C receptor agonists decrease cue-induced reinstatement of cocaine-seeking [3–6] (5-HT2C antagonists have no effect [5, 7]). These latter findings suggest that cue-associated seeking behavior is not dependent on 5-HT levels at synapses expressing 5-HT2C receptors (illustrated by the lack of effect of antagonists on cocaine cue-induced reinstatement); nonetheless, the systems regulating seeking behavior are under negative control by activated 5-HT2C receptors (illustrated by the ability of 5-HT2C agonists to decrease cue-induced reinstatement of cocaine-seeking). The majority of behavioral studies focusing on 5-HT2C receptors have relied on agonist/antagonist relationships. However, evidence indicates constitutive activity (i.e. agonist-independent activation) of 5-HT2C receptors in vivo[8–10] and it currently is unknown how constitutively active 5-HT2C receptors impact stimulant-induced behaviors.
5-HT2C receptors are the only known G protein coupled receptor to undergo mRNA editing by adenosine deaminases [11, 12]; a process that has important implications for the level of constitutive activity. Adenosine to inosine switches result in amino acid substitutions in the second intracellular loop of the receptor protein [13–15]. The unedited 5-HT2C receptor displays high levels of constitutive activity [16–20], and these receptor protein substitutions decrease constitutive activity. Constitutive activity can be attenuated pharmacologically by inverse agonists, these drugs stabilize the receptor in its inactive conformation [21, 22]. For example, systemic administration of the 5-HT2C inverse agonist SB 206553 (SB206) enhances dopamine (DA) efflux in the nucleus accumbens and striatum in rats , whereas an agonist either decreases or has no effect on accumbal and striatal DA [8, 23–27]. Supporting a role of constitutive activity in this effect, the SB206-induced effects were verified to occur independent of endogenous 5-HT . We previously revealed that the atypical antidepressant, mirtazapine, attenuates methamphetamine (meth)-induced sensitization , place conditioning [29–31], and seeking  in rodent models of human substance abuse. Mirtazapine historically has been considered to be an antagonist at 5-HT2C and other receptors [32–34]; however, more recent studies indicate its action as an inverse agonist at constitutively active 5-HT2C receptors [35, 36]. These new observations raised the following questions: Would a more selective 5-HT2C receptor inverse agonist, like mirtazapine, also reduce meth-seeking? A related question is whether seeking for meth is similar to, or differs from, that for cocaine with regard to sensitivity to selective 5-HT2C inverse agonists and/or antagonists?
To answer the above questions, we used two 5-HT2C antagonists of different chemical structures, SB 242084 (SB242) and SDZ Ser 082 (SDZ) as well as the putative 5-HT2C receptor inverse agonist SB206. Dose–response evaluations on meth-seeking behaviors were made for these drugs in using cue reactivity (CR) to meth-associated cues established during a self-administration protocol. We have previously demonstrated that CR offers an effective means to study drug-seeking that, as is more typical in the human scenario, does not depend on prior extinction training . We also revealed that the ability of mirtazapine to alter seeking behavior is similar for CR and cue-induced reinstatement (following extinction) protocols . Finally, to ascertain whether 5-HT2C receptor compounds influenced motor activity, rats tested for CR also underwent motor assessments with acute 5-HT2C ligand pretreatment.
Fifty male Sprague–Dawley rats were purchased from Harlan (Indianapolis, IN), acclimated to housing in our local vivarium for 5 days, and handled a minimum of 3 times prior to surgery. Food and water were provided ad libitum throughout the study. Rats were maintained in accordance with the Guide for Care and Use of Laboratory Animals (National Research Council, Washington DC) and with the guidelines and approval of the Rush University Institutional Animal Care and Use Committee.
5-HT 2C receptors
SDZ Ser 082
Rats were instrumented with a jugular vein catheter under continuous isoflurane anesthesia. Custom built catheters were constructed with silastic tubing (0.3 mm i.d. x 0.64 mm o.d.; Dow Corning Co., Midland, MI) and implanted into the right jugular vein. The distal end of the catheter extended to the mid-scapular region with a metal guide canulae (22 gauge; Plastics One Inc., Roanoke, VA) and anchored to a plastic mesh. Rats were allowed to recover for a minimum of 5 days prior to beginning self-administration procedures. During this time, rats were handled, inspected and weighed daily to ensure that surgical wounds were healing properly (without infection), that normal behavioral grooming/eating patterns were retained, and that preoperative weight was regained.
Cue reactivity testing
Meth-seeking behavior was assessed via CR tests. CR testing consisted of single, 1 hr extinction sessions during which cues (i.e., cue light, time-out house light, and activation of infusion pump) were contingently presented on an FR1 schedule. During CR tests, infusion lines were filled with sterile saline and remained connected to saline-filled syringes (syringes were disconnected from pumps to prevent excessive fluid intake). Meth was not accessible during CR testing. The number of active and inactive lever presses was measured continuously and tallied in 15 min intervals. On day 15, rats were given a 30 min pretreatment of vehicle and tested for baseline meth-seeking (CR1); baseline was used to equally distribute rats into treatment groups. Rats were tested for an additional four CR tests (CR2-5); between each CR test, rats were allowed to self-administer meth for 3 hr/day on an FR5 schedule (referred to as intermittent self-administration; see Figure 1) to prevent extinction training. Rats failing to administer at least 50 % of the number of infusions administered on day 14, for two consecutive days during intermittent self-administration sessions, were removed from the study. Rats were tested for CR after a 30 min pretreatment of 0.1, 0.3, 1.0 mg/kg SDZ and vehicle (treatment group 1) or 1.0, 5.0, 10.0 mg/kg SB206, and vehicle (group 2). The 5-HT2C receptor antagonist, SB242, was tested against the 5-HT2C receptor inverse agonist SB206 in the same rats, wherein SB242 (3.0 mg/kg ip) was administered 45 min, and SB206 (5.0 or 10.0 mg/kg ip) was administered 30 min, prior to the onset of CR testing. Dose order was randomized for all treatment groups. Doses of SB206 and SB242 were guided by literature demonstrating significant neurochemical effects in the nucleus accumbens . Doses of SDZ were selected based on reports revealing an enhancement of the interoceptive cues of cocaine  and cocaine-evoked motor activity in naïve rats . We have previously shown that the described CR paradigm is comparable to cue-induced reinstatement and that pharmacological intervention paradigms similar to those tested in the current report similarly reduce CR and cue-induced reinstatement . Assessments were made at an early stage of withdrawal (24 hr) based on our aforementioned work  as well as evidence indicating that psychostimulant administration dysregulates neuronal function in the nucleus accumbens shell (a region with constitutively active 5-HT2C receptors ) as early as 1–3 days of withdrawal  as well as biochemical indices indicating psychostimulant-induced plasticity in multiple brain regions during early phase withdrawal (1–3 days) using place conditioning paradigms [42, 43] and motor sensitization .
A subset of rats tested for CR dose–response assessments was used to determine the motor effects of SDZ (1.0 mg/kg ip) and SB206 (5.0 and 10.0 mg/kg ip) in the presence and absence of meth (1.0 mg/kg ip). For this study, rats were withdrawn from meth and remained in their home cages during protocol days 28–32 and received no treatment (refer to Figure 1). After this 5 day period, rats were tested for motor activity for 3 consecutive days (days 33–35). All motor assessments were conducted using automated small animal activity boxes (Accuscan Instruments, Columbus, OH) equipped with two banks of photobeams positioned at different heights to characterize motor activity in three dimensional space. Rats were habituated to activity chambers for 1 hr prior to each motor test. On day 33, rats were administered 1 ml/kg of the respective vehicle for each test drug (rats for effects of SDZ were administered saline and rats tested for SB206-induced effects were administered 1 % lactic acid in deionized water). The injected rats were immediately returned to motor boxes for 1 hr after which rats were injected with either SDZ (1.0 mg/kg), or SB206 (5.0 or 10.0 mg/kg) and behavior was recorded for an additional 1 hr. Motor data collected 30 min post-injection were subsequently analyzed; this time frame reflected the one that was relevant to CR behaviors. On day 34, rats were administered a 30 min pretreatment of vehicle (saline or 1 % lactic acid), then administered 1 mg/kg meth (ip) and behavior recorded for 1 hr. On day 35, the procedure from day 34 was repeated using SDZ (1.0 mg/kg), or SB206 (5.0 or 10.0 mg/kg) (ip) instead of respective vehicles. Peak meth effects occurred 15 min post meth injection; meth-evoked motor activity was therefore analyzed for the last 45 min of testing (i.e., 15 min post meth injection). Horizontal activity (number of beam breaks in the horizontal plane), vertical activity (number of beam breaks in the vertical plane indicating rearing-like behavior), and total distance (cm traversed within the chamber) were recorded. These assessments provide a reliable index of overall motor patterns evoked by this dose of meth . Stereotypy (rapid, repetitive behaviors) is a prominent component of meth-induced motor activity ; therefore, stereotypy number (the number of beam breaks repetitively disrupted) also was analyzed for meth-evoked motor activity. Rats tested for effects of SDZ on motor function were also tested for SDZ effects on CR; similarly, rats tested for effects of SB206 on motor function had prior exposure to SB206 during CR assessments.
Active lever presses, inactive lever presses, and number of infusions achieved during self-administration sessions were analyzed using a one-way rmANOVA with Newman-Keuls post-hoc analysis. The differences in lever pressing during CR tests were detected using two-way rmANOVA with Newman-Keuls post-hoc analysis. Lever pressing behavior for days 16 and 26 of intermittent self-administration, and motor activity were analyzed using a paired t-test. For all tests, α = 0.05. Data are presented as mean ± SEM. Data greater than two standard deviations from the mean were considered to be statistical outliers and were excluded from the analyses; for CR, outliers were determined from behavior collapsed across the first 30 min of the tests.
Stable meth self-administration
Meth-seeking behavior: cue reactivity
A within-subjects design was used wherein each rat was tested with three doses (randomized) of either the antagonist (SDZ; n = 11), inverse agonist (SB206; n = 8) and the respective vehicles. Similarly, a within-subjects design was used to test for antagonist/inverse agonist interactions with 3.0 mg/kg SB242 vs. 10.0 (n = 11) and 5.0 mg/kg (n = 10) SB206, and respective vehicles. There were no significant differences among rats assigned to SDZ (treatment group 1), SB206 (treatment group 2), or interaction studies (treatment groups 3 and 4) for active or inactive lever pressing during CR1 (ANOVA, p > 0.05; data not shown). Additionally, active lever pressing comparing CR after vehicle pretreatment was not different among the four treatment groups (ANOVA, p > 0.05; data not shown). Self-administration behavior was not altered by the interposed CR testing sessions; the first and last day of intermittent self-administration were not significantly different for active lever presses, inactive lever presses, or infusions comparing day 16 and day 26 (paired t-test, p > 0.05; Figure 2B). In agreement with our prior report validating consistent self-administration and seeking , these data verify that behavior is persistent throughout the paradigm.
SDZ, the 5-HT2C antagonist, had no effect on number of active (Figure 3A) or inactive lever pressing (data not shown) at any dose tested. Two-way rmANOVA comparing active lever pressing revealed no significant Treatment effect (F(3,36) = 0.81), a significant Time effect (F(1,36) = 97.57), and no Treatment x Time interaction (F(3,36) = 0.49). In contrast, administration of the 5-HT2C inverse agonist SB206 attenuated active lever pressing in the first 15 min at all doses tested (Figure 3B) resulting in an approximately 25 %, 50 %, and 95 % reduction in active lever pressing by 1.0, 5.0, and 10.0 mg/kg, respectively. Two-way rmANOVA revealed a significant effect of Treatment (F(3,24) = 5.97) and Time (F(1,24) = 34.70), and a Treatment x Time interaction (F(3,24) = 5.80). Inactive lever pressing was attenuated by approximately 60, 80, and 100 % for 1.0, 5.0, and 10.0 mg/kg SB206, respectively, in the first 15 min of testing (data not shown). While the percentage change in inactive lever pressing appears dramatic, this only corresponds to approximately 2, 3, and 4 fewer inactive lever presses by 1.0, 5.0, and 10.0 mg/kg, respectively.
Similar to the effects seen with SDZ, 3.0 mg/kg of SB242 (also a 5-HT2C receptor antagonist) had no effect on active (Figure 4) or inactive (data not shown) lever pressing when administered alone. Pretreatment with 3.0 mg/kg SB242 15 min prior to administration of 10.0 or 5.0 mg/kg of SB206 however, did not attenuate the SB206-induced suppression of active (Figure 4) and inactive (data not shown) lever pressing. Two-way rmANOVA revealed a significant Treatment effect (F(3,40) = 6.04), Time effect (F(1,40) = 21.97), and Treatment x Time interaction (F(3,40) = 7.36) comparing the effects of 3.0 mg/kg SB242 and 10.0 mg/kg SB206 as well as a significant Treatment effect (F(3,32) = 4.90), Time effect (F(1,32) = 36.71), and Treatment x Time interaction (F(3,32) = 5.151) comparing the effects of 3.0 mg/kg SB242 and 5.0 mg/kg SB206. Our antagonist data are consistent with the inability of 5-HT2C antagonism to alter cue-induced reinstatement of cocaine-seeking [5, 7]. However, because the SB242 did not prevent SB206-induced decreases in lever pressing, it is unclear whether SB206 effects are 5-HT2C dependent.
Normal exploratory and meth-evoked motor activity
Effects of SDZ Ser 082 and SB 206553 on motor activity
727 ± 85
189 ± 66
46 ± 10
1.0 mg/kg SDZ
572 ± 164
182 ± 65
88 ± 32
634 ± 66
186 ± 63
37 ± 14
5.0 mg/kg SB206
564 ± 132
107 ± 34
9 ± 3
379 ± 97
82 ± 42
32 ± 11
10.0 mg/kg SB206
453 ± 41
132 ± 30
11 ± 5
Meth-evoked motor activity: SDZ Ser 082 and SB 206553 effects
8797 ± 738
3832 ± 336
1142 ± 122
477 ± 33
1.0 mg/kg SDZ
8598 ± 858
3723 ± 392
996 ± 190
460 ± 29
7448 ± 1025
3113 ± 444
797 ± 141
446 ± 29
5.0 mg/kg SB206
9067 ± 711
4326 ± 572
594 ± 120
471 ± 18
8429 ± 1133
3705 ± 692
1124 ± 201
471 ± 26
10.0 mg/kg SB206
6448 ± 1276
2245 ± 499
398 ± 91**
394 ± 58
SB 206553 attenuates methamphetamine-seeking
Results from the current study are the first to investigate the role of 5-HT2C receptor activity in meth-mediated behaviors and the first to test SB206 in a rodent model of addiction. We revealed that the putative inverse agonist robustly and dose-dependently attenuated meth-seeking without impacting exploratory motor activity at 5.0 mg/kg and 10.0 mg/kg. The 5-HT2C antagonists had no effect on either meth-seeking or meth-induced motor activity. These results suggest that our prior work with mirtazapine may reflect the ability of mirtazapine to act as an inverse agonist, not as a 5-HT2C antagonist.
The finding that the antagonist SB242 did not attenuate the effects of the inverse agonist SB206 was unexpected, and raised the question regarding the receptor selectivity of SB206. SB206 has high affinity for both the 5-HT2C and 5-HT2B receptors (pKi = 8.5 and pKi = 8.26, respectively in HEK-293 or CHO-K1 cells expressing human recombinant 5-HT2C or 5-HT2B receptors; Table 1)  with 100 fold or greater selectivity over other receptor targets including 5-HT2A receptors . In the current study, SB206 may antagonize 5-HT2B receptors, which are known to regulate behavioral and DA-enhancing effects of amphetamine . However, the increase in accumbal DA seen with 5.0 mg/kg SB206 is not altered with coadministration of the 5-HT2B antagonist LY 266097 indicating that 5.0 mg/kg SB206 does not substantially antagonize 5-HT2B receptors .
Antagonism of 5-HT2A receptors are a third 5-HT receptor that may be contributing to our findings. 5-HT2A antagonism with M100907 attenuates cue-induced reinstatement of cocaine-seeking  and meth-seeking (unpublished data). As mentioned above, SB206 demonstrates 100 fold selectivity over the 5-HT2A receptor. 5-HT2A antagonism by SB206, particularly at 10.0 mg/kg, is plausible but unlikely at 1.0 and 5.0 mg/kg, for amphetamine-evoked DA concentrations in the nucleus accumbens and striatum are attenuated by 5-HT2A antagonism but 5.0 mg/kg SB206 has no effect  suggesting that at this dose, SB206 does not act on 5-HT2A receptors. Additionally, 5.0 mg/kg SB206 and 5-HT2A antagonism with SR 46349B have oppositional effects demonstrated by striatal 11 C]raclopride binding lending further evidence to the selectivity of SB206 for 5-HT2C over 5-HT2A receptors . Taken together, the available in vivo data suggests that SB206 does not antagonize 5-HT2A receptors at 5.0 mg/kg.
SB206 may also act as a positive allosteric modulator of the α7 nicotinic acetylcholine receptor with an EC50 of 1.5 μM for potentiation of responses evoked in GH4C1 cells by EC20 nicotine . Dunlop et al., find that 3, 10, and 30 mg/kg SB206 reverses MK-801-induced deficits in prepulse inhibition, a classical model of schizophrenia, but only confirm actions as a positive allosteric modulator using a nicotinic antagonist against 10.0 mg/kg SB206. It is unclear whether lower doses, such as 1.0 and 5.0 mg/kg SB206, would also act as a positive allosteric modulator at α7 nicotinic receptors. Consistent with our findings, antagonism of α7 nicotinic receptors in the ventral tegmental area attenuates the reward-facilitating effect of cocaine  whereas nicotine administration attenuates reinstatement of meth-seeking . The role of α7 nicotinic receptors and in particular, positive allosteric modulation of this receptor, in meth abuse is understudied. Future investigation are needed to determine if this receptor is engaged by SB206 in the dose range tested before actions at the α7 receptor can be ruled out.
Debating receptor specificity based on our findings is difficult; however, it is important to note that a prior report showed that 1.0 mg/kg SB242 attenuates 5.0 mg/kg SB206-induced increases in striatal and accumbal DA . Reasons why Spampinato and colleagues were able to antagonize SB206 effects and we were not may relate to the treatment history of rodent subjects. In the aforementioned neurochemical study , subjects were stimulant naïve, halothane anesthetized rats. In our study, rats had an extensive history of meth self-administration. This chronic meth history may have modified the 5-HT2C system, for we have observed a functionally upregulated response to systemic 5-HT2A/C receptor agonism in the ventral pallidum of rats chronically administered meth . Repeated meth administration may result in changes in mRNA editing profiles (and thus, levels of constitutive activity). Proof of concept for this hypothesis is seen with studies on stress, antidepressant, and antipsychotic administration where mRNA editing of the 5-HT2C receptor is altered [55–58]. In addition to changing constitutive activity, mRNA editing of the 5-HT2C receptor also alters the binding affinity of ligands whereby increased editing most often results in decreased agonist affinity for the receptor with enhanced or no effect on antagonists and inverse agonist binding affinity [16–18, 20]. It is unknown how editing patterns affect the affinities of SB206 and SB242. Thus, the inability of SB242 to augment the effects of SB206 on CR may reflect changes in affinity for constitutively active isoforms and/or in the allosteric constant (i.e., an index of the concentration of constitutively active vs. non-constitutively active receptors). Unfortunately ex vivo binding studies, which would greatly facilitate our studies, are hampered by a lack of available tritiated ligands with 5-HT2C specificity. While future studies are needed to explore the mechanism of action for SB206 and determine receptor specificity in vivo, we speculate that despite a lack of antagonism by SB242, 5-HT2C receptors may still be involved. Moreover, continued study is necessary to determine the effects of 5-HT2C receptor ligands on neuron function; to provide further insights our laboratory is conducting patch clamp studies in ex vivo slice preparations to determine the effects of 5-HT2C ligands, including SB206, on neurophysiology.
SB 206553, but not SDZ Ser 082, attenuates methamphetamine-evoked motor activity
Acute antagonism of 5-HT2C receptors enhances the motor effects of acute cocaine administration; however, this enhancement is lost in rats that are motorically sensitized by repeated injections of cocaine . This is comparable to our current findings wherein rats with a history of meth self-administration, 5-HT2C antagonism did not alter meth-evoked motor activity in rats. A single study testing SB206 on cocaine-evoked motor activity finds an attenuation of cocaine-evoked motor activity by 1.0 mg/kg SB206 but a potentiation with 4.0 mg/kg ; however, this was an acute study with a single injection of cocaine making it difficult to compare with the current investigations using meth self-administration. In rats with a history of meth self-administration, only meth-evoked vertical activity was attenuated by 10.0 mg/kg SB206. Both our findings and  are in agreement in that SB206 does not alter normal exploratory motor behavior suggesting that SB206 is not sedating; differences in our study and  regarding the ability to augment psychostimulant-evoked motor activity may reflect behavioral differences consequent to meth-induced plasticity. This hypothesis is supported by  and our current findings wherein 5-HT2C antagonism fails to augment cocaine-evoked and meth-evoked motor activity in rats with a history of repeated cocaine  or meth administration.
SB 206553 decreases inactive lever pressing
Antagonists had no effect, whereas SB206 attenuated inactive lever pressing. Mirtazapine, also an inverse agonist at the 5-HT2C receptor [35, 36], similarly attenuates inactive lever pressing in a cue-induced reinstatement paradigm without effecting non-operant related motor activity in a small animal activity chamber in rats with a history of meth self-administration or rotorod performance in naïve rats . Moreover, in naïve rats, administration of 1, 3, and 4 mg/kg SB206 has no effect on motor activity (measured in activity chambers) . A similar pattern is found for GABAB receptor activation. The GABAB agonist (CGP44542) decreases inactive lever pressing in an operant task , yet has no effect on performance in an intracranial self-stimulation procedure . In summary, as SB206 had no effect on motor activity in in rats with a meth history (current report) or in naïve rats , and that mirtazapine, also an inverse agonist at 5-HT2C receptors [35, 36], does not impair motor activity in naïve rats , we considered that decreased lever pressing of both active and inactive levers seen in the current study was not a consequence of decreased motor activity. Accordingly, we propose that SB206 blunted the salience of the levers, both active and inactive, rather than impairing the ability of the rats to perform the operant task. This interpretation is supported by our prior demonstration that significance of meth-associated context cues are reduced by mirtazapine . Additional studies aimed at exploring the effect of SB206 on non-drug reinforced behaviors may advance this hypothesis; however, as 5-HT2C receptors regulate food intake [6, 62, 63] as well as intracranial self-stimulation behavior [64, 65], such evaluations may challenging to conduct and difficult to interpret.
The presented findings are the first to explore the effects of 5-HT2C antagonists and putative inverse agonist, SB206, on meth-seeking and meth-induced motor activity. Serotonin2C antagonism had no effect on meth-seeking or motor activity (in the presence or absence of meth); in contrast, SB206, attenuated meth-seeking. This effect was selective, as SB206 agonist did not alter exploratory behavior, and only the higher dose reduced one motor parameter evoked by meth (i.e., vertical activity). There is a growing interest in assessing the role of constitutively active 5-HT2C receptors in vivo. It is currently unclear whether SB206 elicits the observed effects via 5-HT2C receptor inverse agonism; nonetheless, based on the dramatic reduction in seeking behavior, further investigation of this compound is merited as well as continued study of 5-HT2C regulation of psychostimulant-mediated behaviors.
SDZ Ser 082
Work supported by USPHSG DA015760 to TCN and DA024923 to SMG and TCN and the Daniel F. and Ada L. Rice Foundation.
- Higgins GA, Fletcher PJ: Serotonin and drug reward: focus on 5-HT2C receptors. Eur J Pharmacol. 2003, 480: 151-162. 10.1016/j.ejphar.2003.08.102.PubMedView ArticleGoogle Scholar
- Hayes DJ, Greenshaw AJ: 5-HT receptors and reward-related behaviour: a review. Neurosci Biobehav Rev. 2011, 35: 1419-1449. 10.1016/j.neubiorev.2011.03.005.PubMedView ArticleGoogle Scholar
- Fletcher PJ, Grottick AJ, Higgins GA: Differential effects of the 5-HT(2A) receptor antagonost M100,907 and the 5-HT(2 C) receptor antagonist SB242,084 on cocaine-iduced locomotor activity, cocaine self-administration and cocaine-induced reinstatement of responding. Neuropschopharmacology. 2002, 27: 576-586.Google Scholar
- Burbassi S, Cervo L: Stimulation of serotonin2C receptors influences cocaine-seeking behavior in response to drug-associated stimuli in rats. Psychopharmacology (Berl). 2008, 196: 15-27. 10.1007/s00213-007-0916-7.View ArticleGoogle Scholar
- Neisewander JL, Acosta JI: Stimulation of 5-HT2C receptors attenuates cue and cocaine-primed reinstatement of cocaine-seeking behavior in rats. Behav Pharmacol. 2007, 18: 791-800. 10.1097/FBP.0b013e3282f1c94b.PubMedView ArticleGoogle Scholar
- Cunningham KA, Fox RG, Anastasio NC, Bubar MJ, Stutz SJ, Moeller FG, Gilbertson SR, Rosenzweig-Lipson S: Selective serotonin 5-HT(2C) receptor activation suppresses the reinforcing efficacy of cocaine and sucrose but differentially affects the incentive-salience value of cocaine- vs. sucrose-associated cues. Neuropharmacology. 2011, 61: 513-523. 10.1016/j.neuropharm.2011.04.034.PubMed CentralPubMedView ArticleGoogle Scholar
- Filip M: Role of serotonin (5-HT)2 receptors in cocaine self-administration and seeking behavior in rats. Pharmacol Rep. 2005, 57: 35-46.PubMedGoogle Scholar
- De Deurwaerdere P, Navailles S, Berg KA, Clarke WP, Spampinato U: Constitutive activity of the serotonin2C receptor inhibits in vivo dopamine release in the rat striatum and nucleus accumbens. J Neurosci. 2004, 24: 3235-3241. 10.1523/JNEUROSCI.0112-04.2004.PubMedView ArticleGoogle Scholar
- Leggio GM, Cathala A, Neny M, Rouge-Pont F, Drago F, Piazza PV, Spampinato U: In vivo evidence that constitutive activity of serotonin(2C) receptors in the medial prefrontal cortex participates in the control of dopamine release in the rat nucleus accumbens: differential effects of inverse agonist versus antagonist. J Neurochem. 2009Google Scholar
- Navailles S, Moison D, Ryczko D, Spampinato U: Region-dependent regulation of mesoaccumbens dopamine neurons in vivo by the constitutive activity of central serotonin2C receptors. J Neurochem. 2006, 99: 1311-1319. 10.1111/j.1471-4159.2006.04188.x.PubMedView ArticleGoogle Scholar
- Burns CM, Chu H, Rueter SM, Hutchinson LK, Canton H, Sanders-Bush E, Emeson RB: Regulation of serotonin-2 C receptor G-protein coupling by RNA editing. Nature. 1997, 387: 303-308. 10.1038/387303a0.PubMedView ArticleGoogle Scholar
- Niswender CM, Sanders-Bush E, Emeson RB: Identification and characterization of RNA editing events within the 5-HT2C receptor. Ann N Y Acad Sci. 1998, 861: 38-48. 10.1111/j.1749-6632.1998.tb10171.x.PubMedView ArticleGoogle Scholar
- Sanders-Bush E, Fentress H, Hazelwood L: Serotonin 5-ht2 receptors: molecular and genomic diversity. Mol Interv. 2003, 3: 319-330. 10.1124/mi.3.6.319.PubMedView ArticleGoogle Scholar
- Werry TD, Loiacono R, Sexton PM, Christopoulos A: RNA editing of the serotonin 5HT2C receptor and its effects on cell signalling, pharmacology and brain function. Pharmacol Ther. 2008, 119: 7-23. 10.1016/j.pharmthera.2008.03.012.PubMedView ArticleGoogle Scholar
- Gardiner K, Du Y: A-to-I editing of the 5HT2C receptor and behaviour. Brief Funct Genomic Proteomic. 2006, 5: 37-42. 10.1093/bfgp/ell006.PubMedView ArticleGoogle Scholar
- Fitzgerald LW, Iyer G, Conklin DS, Krause CM, Marshall A, Patterson JP, Tran DP, Jonak GJ, Hartig PR: Messenger RNA editing of the human serotonin 5-HT2C receptor. Neuropsychopharmacology. 1999, 21: 82S-90S.PubMedView ArticleGoogle Scholar
- Herrick-Davis K, Grinde E, Niswender CM: Serotonin 5-HT2C receptor RNA editing alters receptor basal activity: implications for serotonergic signal transduction. J Neurochem. 1999, 73: 1711-1717.PubMedView ArticleGoogle Scholar
- Niswender CM, Copeland SC, Herrick-Davis K, Emeson RB, Sanders-Bush E: RNA editing of the human serotonin 5-hydroxytryptamine 2 C receptor silences constitutive activity. J Biol Chem. 1999, 274: 9472-9478. 10.1074/jbc.274.14.9472.PubMedView ArticleGoogle Scholar
- Berg KA, Cropper JD, Niswender CM, Sanders-Bush E, Emeson RB, Clarke WP: RNA-editing of the 5-HT(2 C) receptor alters agonist-receptor-effector coupling specificity. Br J Pharmacol. 2001, 134: 386-392. 10.1038/sj.bjp.0704255.PubMed CentralPubMedView ArticleGoogle Scholar
- Berg KA, Dunlop J, Sanchez T, Silva M, Clarke WP: A conservative, single-amino acid substitution in the second cytoplasmic domain of the human Serotonin2C receptor alters both ligand-dependent and -independent receptor signaling. J Pharmacol Exp Ther. 2008, 324: 1084-1092.PubMedView ArticleGoogle Scholar
- Kenakin T: Drug efficacy at G protein-coupled receptors. Annu Rev Pharmacol Toxicol. 2002, 42: 349-379. 10.1146/annurev.pharmtox.42.091401.113012.PubMedView ArticleGoogle Scholar
- Kenakin T: Principles: receptor theory in pharmacology. Trends Pharmacol Sci. 2004, 25: 186-192. 10.1016/j.tips.2004.02.012.PubMedView ArticleGoogle Scholar
- Willins DL, Meltzer HY: Serotonin 5-HT2Cagonists selectively inhibit morphine-induced dopamine efflux in the nucleus accumbens. Brain Research. 1998, 781: 291-299. 10.1016/S0006-8993(97)01267-5.PubMedView ArticleGoogle Scholar
- Lucas G, Spampinato U: Role of striatal serotonin2A and serotonin2C receptor subtypes in the control of in vivo dopamine outflow in the rat striatum. J Neurochem. 2000, 74: 693-701.PubMedView ArticleGoogle Scholar
- Gobert A, Rivet JM, Lejeune F, Newman-Tancredi A, Ddhumeau-Auclair A, Nicolas JP, Cistarelli L, Melon C, Millan MJ: Serotonin(2C) receptors tonically suppress the activity of mesocortical dopaminergic and adrenergic, but not serotonergic, pathways: a combined dialysis and electrophysiological analysis in the rat. Synapse. 2000, 36: 205-221. 10.1002/(SICI)1098-2396(20000601)36:3<205::AID-SYN5>3.0.CO;2-D.PubMedView ArticleGoogle Scholar
- Di Matteo V, Di Giovanni G, Di Mascio M, Esposito E: Selective blockade of serotonin2C/2Breceptors enhances dopamine release in the rat nucleus accumbens. Neuropharmacology. 1998, 37: 265-272. 10.1016/S0028-3908(98)00014-8.PubMedView ArticleGoogle Scholar
- Di Matteo V, Di Mascio M, Di Giovanni G, Esposito E: Acute administration of amitriptyline and mianserin increases dopamine release in the rat nucleus accumbens: possible involvement of serotonin2Creceptors. Psychopharmacology. 2000, 150: 45-51. 10.1007/s002130000420.PubMedView ArticleGoogle Scholar
- McDaid J, Tedford CE, Mackie AR, Dallimore JE, Mickiewicz AL, Shen F, Angle JM, Napier TC: Nullifying drug-induced sensitization: behavioral and electrophysiological evaluations of dopaminergic and serotonergic ligands in methamphetamine-sensitized rats. Drug Alcohol Depend. 2007, 86: 55-66. 10.1016/j.drugalcdep.2006.05.014.PubMedView ArticleGoogle Scholar
- Voigt RM, Napier TC: Context-dependent effects of a single administration of mirtazapine on the expression of methamphetamine-induced conditioned place preference. Front Behav Neurosci. 2011, 5: 92.PubMed CentralPubMedGoogle Scholar
- Voigt RM, Mickiewicz AL, Napier TC: Repeated mirtazapine nullifies the maintenance of previously established methamphetamine-induced conditioned place preference in rats. Behav Brain Res. 2011, 225: 91-96. 10.1016/j.bbr.2011.07.009.PubMed CentralPubMedView ArticleGoogle Scholar
- Herrold AA, Shen F, Graham MP, Harper LK, Specio SE, Tedford CE, Napier TC: Mirtazapine treatment after conditioning with methamphetamine alters subsequent expression of place preference. Drug Alcohol Depend. 2009, 99: 231-239. 10.1016/j.drugalcdep.2008.08.005.PubMedView ArticleGoogle Scholar
- Kooyman AR, Zwart R, Vanderheijden PM, Van Hooft JA, Vijverberg HP: Interaction between enantiomers of mianserin and ORG3770 at 5-HT3 receptors in cultured mouse neuroblastoma cells. Neuropharmacology. 1994, 33: 501-507. 10.1016/0028-3908(94)90081-7.PubMedView ArticleGoogle Scholar
- de Boer TH, Maura G, Raiteri M, de Vos CJ, Wieringa J, Pinder RM: Neurochemical and autonomic pharmacological profiles of the 6-aza- analogue of mianserin, Org 3770 and its enantiomers. Neuropharmacology. 1988, 27: 399-408. 10.1016/0028-3908(88)90149-9.PubMedView ArticleGoogle Scholar
- Millan MJ, Gobert A, Rivet JM, Adhumeau-Auclair A, Cussac D, Newman-Tancredi A, Dekeyne A, Nicolas JP, Lejeune F: Mirtazapine enhances frontocortical dopaminergic and corticolimbic adrenergic, but not serotonergic, transmission by blockade of alpha2- adrenergic and serotonin2C receptors: a comparison with citalopram. Eur J Neurosci. 2000, 12: 1079-1095. 10.1046/j.1460-9568.2000.00982.x.PubMedView ArticleGoogle Scholar
- Chanrion B, la Mannoury CC, Gavarini S, Seimandi M, Vincent L, Pujol JF, Bockaert J, Marin P, Millan MJ: Inverse agonist and neutral antagonist actions of antidepressants at recombinant and native 5-hydroxytryptamine2C receptors: differential modulation of cell surface expression and signal transduction. Mol Pharmacol. 2008, 73: 748-757.PubMedView ArticleGoogle Scholar
- Labasque M, Meffre J, Carrat G, Becamel C, Bockaert J, Marin P: Constitutive Activity of Serotonin2C Receptors at G Protein-Independent Signaling: Modulation by RNA Editing and Antidepressants. Mol Pharmacol. 2010Google Scholar
- Graves SM, Napier TC: Mirtazapine alters cue-associated methamphetamine seeking in rats. Biol Psychiatry. 2011, 69: 275-281. 10.1016/j.biopsych.2010.09.032.PubMed CentralPubMedView ArticleGoogle Scholar
- Acosta JI, Thiel KJ, Sanabria F, Browning JR, Neisewander JL: Effect of schedule of reinforcement on cue-elicited reinstatement of cocaine-seeking behavior. Behav Pharmacol. 2008, 19: 129-136. 10.1097/FBP.0b013e3282f62c89.PubMedView ArticleGoogle Scholar
- Filip M, Bubar MJ, Cunningham KA: Contribution of serotonin (5-HT) 5-HT2 receptor subtypes to the discriminative stimulus effects of cocaine in rats. Psychopharmacology (Berl). 2006, 183: 482-489. 10.1007/s00213-005-0197-y.View ArticleGoogle Scholar
- Filip M, Bubar MJ, Cunningham KA: Contribution of serotonin (5-hydroxytryptamine; 5-HT) 5-HT2 receptor subtypes to the hyperlocomotor effects of cocaine: acute and chronic pharmacological analyses. J Pharmacol Exp Ther. 2004, 310: 1246-1254. 10.1124/jpet.104.068841.PubMedView ArticleGoogle Scholar
- Kourrich S, Thomas MJ: Similar neurons, opposite adaptations: psychostimulant experience differentially alters firing properties in accumbens core versus shell. J Neurosci. 2009, 29: 12275-12283. 10.1523/JNEUROSCI.3028-09.2009.PubMed CentralPubMedView ArticleGoogle Scholar
- Rademacher DJ, Napier TC, Meredith GE: Context modulates the expression of conditioned motor sensitization, cellular activation and synaptophysin immunoreactivity. Eur J Neurosci. 2007, 26: 2661-2668. 10.1111/j.1460-9568.2007.05895.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Rademacher DJ, Kovacs B, Shen F, Napier TC, Meredith GE: The neural substrates of amphetamine conditioned place preference: implications for the formation of conditioned stimulus-reward associations. Eur J Neurosci. 2006, 24: 2089-2097. 10.1111/j.1460-9568.2006.05066.x.PubMedView ArticleGoogle Scholar
- McDaid J, Graham MP, Napier TC: Methamphetamine-induced sensitization differentially alters pCREB and ΔFosB throughtout the limbic circuit of the mammalian brain. Mol Pharmacol. 2006, 70: 2064-2074. 10.1124/mol.106.023051.PubMedView ArticleGoogle Scholar
- Knight AR, Misra A, Quirk K, Benwell K, Revell D, Kennett G, Bickerdike M: Pharmacological characterisation of the agonist radioligand binding site of 5-HT(2A), 5-HT(2B) and 5-HT(2 C) receptors. Naunyn Schmiedebergs Arch Pharmacol. 2004, 370: 114-123.PubMedView ArticleGoogle Scholar
- Kennett GA, Wood MD, Bright F, Cilia J, Piper DC, Gager T, Thomas D, Baxter GS, Forbes IT, Ham P, et al: In vitro and in vivo profile of SB 206553, a potent 5-HT2C/5-HT2B receptor antagonist with anxiolytic-like properties. Br J Pharmacol. 1996, 117: 427-434. 10.1111/j.1476-5381.1996.tb15208.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Auclair AL, Cathala A, Sarrazin F, Depoortere R, Piazza PV, Newman-Tancredi A, Spampinato U: The central serotonin receptor: a new pharmacological target to modulate the mesoaccumbens dopaminergic pathway activity. J Neurochem. 2010, 114: 1323-1332.PubMedGoogle Scholar
- Nic Dhonnchadha BA, Fox RG, Stutz SJ, Rice KC, Cunningham KA: Blockade of the serotonin 5-ht2a receptor suppresses cue-evoked reinstatement of cocaine-seeking behavior in a rat self-administration model. Behav Neurosci. 2009, 123: 382-396.PubMedView ArticleGoogle Scholar
- Porras G, Di MV, Fracasso C, Lucas G, De DP, Caccia S, Esposito E, Spampinato U: 5-HT2A and 5-HT2C/2B receptor subtypes modulate dopamine release induced in vivo by amphetamine and morphine in both the rat nucleus accumbens and striatum. Neuropsychopharmacology. 2002, 26: 311-324. 10.1016/S0893-133X(01)00333-5.PubMedView ArticleGoogle Scholar
- Egerton A, Ahmad R, Hirani E, Grasby PM: Modulation of striatal dopamine release by 5-HT2A and 5-HT2C receptor antagonists: [11 C]raclopride PET studies in the rat. Psychopharmacology (Berl). 2008, 200: 487-496. 10.1007/s00213-008-1226-4.View ArticleGoogle Scholar
- Dunlop J, Lock T, Jow B, Sitzia F, Grauer S, Jow F, Kramer A, Bowlby MR, Randall A, Kowal D, et al: Old and new pharmacology: positive allosteric modulation of the alpha7 nicotinic acetylcholine receptor by the 5-hydroxytryptamine(2B/C) receptor antagonist SB-206553 (3,5-dihydro-5-methyl-N-3-pyridinylbenzo[1,2-b:4,5-b']di pyrrole-1(2 H)-carboxamide). J Pharmacol Exp Ther. 2009, 328: 766-776. 10.1124/jpet.108.146514.PubMedView ArticleGoogle Scholar
- Panagis G, Kastellakis A, Spyraki C, Nomikos G: Effects of methyllycaconitine (MLA), an alpha 7 nicotinic receptor antagonist, on nicotine- and cocaine-induced potentiation of brain stimulation reward. Psychopharmacology (Berl). 2000, 149: 388-396. 10.1007/s002130000384.View ArticleGoogle Scholar
- Hiranita T, Nawata Y, Sakimura K, Anggadiredja K, Yamamoto T: Suppression of methamphetamine-seeking behavior by nicotinic agonists. Proc Natl Acad Sci U S A. 2006, 103: 8523-8527. 10.1073/pnas.0600347103.PubMed CentralPubMedView ArticleGoogle Scholar
- Napier TC, Istre ED: Methamphetamine-induced sensitization includes a functional upregulation of ventral pallidal 5-HT2A/2 C receptors. Synapse. 2008, 62: 14-21. 10.1002/syn.20460.PubMedView ArticleGoogle Scholar
- Huang XF, Tan YY, Huang X, Wang Q: Effect of chronic treatment with clozapine and haloperidol on 5-HT(2A and 2 C) receptor mRNA expression in the rat brain. Neurosci Res. 2007, 59: 314-321. 10.1016/j.neures.2007.08.001.PubMedView ArticleGoogle Scholar
- Du Y, Stasko M, Costa AC, Davisson MT, Gardiner KJ: Editing of the serotonin 2 C receptor pre-mRNA: Effects of the Morris Water Maze. Gene. 2007, 391: 186-197. 10.1016/j.gene.2006.12.023.PubMed CentralPubMedView ArticleGoogle Scholar
- Iwamoto K, Nakatani N, Bundo M, Yoshikawa T, Kato T: Altered RNA editing of serotonin 2 C receptor in a rat model of depression. Neurosci Res. 2005, 53: 69-76. 10.1016/j.neures.2005.06.001.PubMedView ArticleGoogle Scholar
- Englander MT, Dulawa SC, Bhansali P, Schmauss C: How stress and fluoxetine modulate serotonin 2 C receptor pre-mRNA editing. J Neurosci. 2005, 25: 648-651. 10.1523/JNEUROSCI.3895-04.2005.PubMedView ArticleGoogle Scholar
- McCreary AC, Cunningham KA: Effects of the 5-HT2C/2B antagonist SB 206553 on hyperactivity induced by cocaine. Neuropsychopharmacology. 1999, 20: 556-564. 10.1016/S0893-133X(98)00087-6.PubMedView ArticleGoogle Scholar
- Paterson NE, Froestl W, Markou A: The GABAB receptor agonists baclofen and CGP44532 decreased nicotine self-administration in the rat. Psychopharmacology (Berl). 2004, 172: 179-186. 10.1007/s00213-003-1637-1.View ArticleGoogle Scholar
- Paterson NE, Markou A: The metabotropic glutamate receptor 5 antagonist MPEP decreased break points for nicotine, cocaine and food in rats. Psychopharmacology. 2004Google Scholar
- Fletcher PJ, Sinyard J, Higgins GA: Genetic and pharmacological evidence that 5-HT2C receptor activation, but not inhibition, affects motivation to feed under a progressive ratio schedule of reinforcement. Pharmacol Biochem Behav. 2010, 97: 170-178. 10.1016/j.pbb.2010.07.002.PubMedView ArticleGoogle Scholar
- Fletcher PJ, Tampakeras M, Sinyard J, Slassi A, Isaac M, Higgins GA: Characterizing the effects of 5-HT(2 C) receptor ligands on motor activity and feeding behaviour in 5-HT(2 C) receptor knockout mice. Neuropharmacology. 2009, 57: 259-267. 10.1016/j.neuropharm.2009.05.011.PubMedView ArticleGoogle Scholar
- Hayes DJ, Clements R, Greenshaw AJ: Effects of systemic and intra-nucleus accumbens 5-HT2C receptor compounds on ventral tegmental area self-stimulation thresholds in rats. Psychopharmacology (Berl). 2009, 203: 579-588. 10.1007/s00213-008-1404-4.View ArticleGoogle Scholar
- Katsidoni V, Apazoglou K, Panagis G: Role of serotonin 5-HT2A and 5-HT2C receptors on brain stimulation reward and the reward-facilitating effect of cocaine. Psychopharmacology (Berl). 2011, 213: 337-354. 10.1007/s00213-010-1887-7.View ArticleGoogle Scholar
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