Extracellular ATP activates NFAT-dependent gene expression in neuronal PC12 cells via P2X receptors
© Prasai et al; licensee BioMed Central Ltd. 2011
Received: 8 May 2011
Accepted: 23 September 2011
Published: 23 September 2011
Treatment of neuronal PC12 cells with ATP induces depolarisation and increases intracellular calcium levels via purinergic receptors. In many cell types, sustained elevation of intracellular calcium levels cause changes in gene expression via activation of the transcription factor NFAT (nuclear factor of activated T cells). We have therefore characterised the signalling pathway by which ATP regulates NFAT-dependent gene expression in PC12 cells.
The activation of NFAT transcriptional activity by extracellular ATP was characterised with the help of reporter gene assays. Treatment of PC12 cells with ATP elicited a dose-dependent increase in luciferase activity (EC50 = 78 μM). UTP, 4-benzoylbenzoyl ATP and α,β-methylene ATP did not mimic the effect of ATP, which was abolished by treatment with the P2X receptor antagonist pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS). This pharmacological characterisation provides evidence for a critical role of ionotropic P2X receptors. Blockade of L-type voltage-dependent calcium channels by nifedipine reduced the response of NFAT to ATP, indicating that a depolarisation-mediated calcium influx was required for maximal NFAT activation. Inhibition of store-operated calcium entry by the pyrazole derivative BTP2 also diminished ATP-dependent NFAT activation. Furthermore, ATP-induced NFAT activation was associated with the activation of the mitogen-activated protein kinases ERK1/2. Finally, treatment with ATP increased the levels of the NFAT target transcripts, RCAN1-4 (regulator of calcineurin) and BDNF (brain derived neurotrophic factor).
The present data show that ATP induces NFAT-dependent changes in gene expression in PC12 cells by acting on P2X receptors. Maximal NFAT activation depends on both depolarisation-induced calcium influx and store-operated calcium entry and requires the activity of the protein phosphatase calcineurin and the mitogen-activated protein kinase cascade.
Purinergic signalling plays a significant role in neurotransmission and neuromodulation in many regions of the brain as well as in the spinal cord and peripheral neurons [1, 2]. Among purinergic agonists, extracellular ATP is a potent signalling molecule abundantly present in the central nervous system. ATP is secreted from many neurons as a cotransmitter by vesicular exocytotic release, but also leaks from injured or dying cells . In addition, many cell types, including glial cells, can also release ATP in response to stimuli such as hypoxia or certain agonists independently of cell damage and thereby modulate the function of adjacent neurons.
Once released into the extracellular space, ATP acts on specific receptors that belong to two main subclasses: ligand-gated P2X cation-selective channels and G protein-coupled P2Y receptors . Both receptor classes evoke calcium responses. P2X receptors can induce depolarisation-induced calcium entry and are permeable to Ca2+, whereas most P2Y receptors couple to phospholipase Cβ isoforms, which leads to the release of Ca2+ from internal stores. Purinergic effects mediated by Ca2+ signalling include presynaptic neurotransmitter release, hormone secretion, calcium wave propagation between astrocytes and activation of primary nociceptive neurons [3, 5–7].
Although purinergic receptors are abundant in the nervous system and have been extensively characterised with respect to their short-term effects on neuronal function, less is known about the long-term effects of their activation in neurons. There is evidence that extracellular nucleotides affect neuronal differentiation and survival, but the signalling pathways that mediate these effects are largely unexplored . Specifically, the increase in intracellular Ca2+ concentrations after activation of purinergic receptors is expected to influence gene expression. The calcineurin-NFAT (nuclear factor of activated T-cells) pathway is a major mediator of Ca2+ effects on gene expression in neuronal cells and plays a key role in neuronal development and function [9–11]. Surprisingly, the effects of purinergic receptors on NFAT signalling and NFAT-dependent gene expression have not yet been studied in neuronal cells.
The rat pheochromocytoma cell line PC12 is a well-characterised model system for purinergic effects. PC12 cells express P2X and P2Y receptors and show increases in intracellular Ca2+ concentration upon stimulation with extracellular ATP [12–16]. Extracellular ATP stimulates catecholamine release from PC12 cells, enhances their sensitivity to nerve growth factor, promotes neurite outgrowth and regulates cytoskeleton remodelling [13, 15, 17, 18]. Moreover, PC12 cells express the components of the calcineurin-NFAT pathway and have been used to characterise NFAT-dependent changes in gene expression [19–21].
Here we have tested the hypothesis that extracellular ATP can modulate gene expression in neuronal cells via the calcineurin-NFAT pathway. We show that ATP stimulates NFAT transcriptional activity through the activation of P2X receptors, causes the activation of ERK1/2 kinases and induces the expression of an NFAT target gene in PC12 cells. These results suggest that extracellular ATP can act on neuronal cells by inducing NFAT-dependent changes in gene expression.
Extracellular ATP induces NFAT-dependent reporter gene activity in PC12 cells
Pharmacological characterisation of purinergic receptors that mediate NFAT activation in PC12 cells
To further examine the receptors responsible for NFAT activation in PC12 cells, we used the P2X receptor antagonist PPADS (Figure 2B). Treatment of the PC12-NFAT-Luc cells with 10 μM PPADS strongly suppressed the induction of luciferase activity by ATP, suggesting that at least one of the PPADS-sensitive P2X subunits (P2X1, P2X2, P2X3 or P2X5; ) is involved in NFAT activation.
Expression analysis of P2X receptor subunits and NFAT isoforms in PC12 cells
Mechanisms of cytosolic Ca2+ increase
To test the latter possibility, we studied the effect of the L-type calcium channel blocker, nifedipine, on the induction of luciferase by ATP at the optimal concentration of ATP in this assay (300 μM) as well as a suboptimal concentration of 150 μM ATP (Figure 4A). Nifedipine strongly reduced NFAT activation but did not completely prevent the effect of ATP, indicating that a major part of the NFAT response depends on Ca2+ influx through L-type Ca2+ channels.
Next we asked whether store-operated Ca2+ entry (SOCE) contributed to the effect of ATP on NFAT activation. The pyrazole derivative BTP2 is a blocker of SOCE and inhibits NFAT effects in different cell types, including T lymphocytes and cardiomyocytes [26, 27]. Treatment with BTP2 reduced NFAT activation in PC12 cells in a concentration-dependent manner (Figure 4B). Partial but significant inhibition was observed at submicromolar concentrations (0.3 μM), at which BTP2 is thought to specifically inhibit SOCE [28, 29]. A maximal effect of 72% inhibition was observed at a concentration of 30 μM BTP2. It must be noted that the direct molecular target(s) of BTP2 are still not well defined , and the unsteady slope of the concentration-response curve might suggest that there is more than one target affected by BTP2. Taken together, these results suggest that the maximal activation of NFAT by extracellular ATP in PC12 cells requires the influx of extracellular Ca2+ ions both via voltage-dependent calcium channels and a BTP2-sensitive mechanism.
Pathway for the ATP activation of NFAT
Effect of extracellular ATP on NFAT target genes
Our results show that the Ca2+ response elicited by extracellular ATP in neuronal cells translates into changes in gene expression that are mediated by the transcription factor NFAT. In the PC12 cells that we used as a model system, NFAT activation by ATP required the influx of Ca2+ from the extracellular space and depended on the activation of P2X receptors and the function of L-type voltage-dependent Ca2+ channels. The calcineurin-NFAT pathway plays an important role in neuronal development, and our results support the assumption that purinergic receptors transmit some of their trophic effects by this mechanism .
Secondly, the pharmacological characterisation of the purinergic receptor responsible for NFAT activation supports the hypothesis that a P2X receptor is critically involved. At a concentration of 10 μM, PPADS is an antagonist of homo- or heteromeric P2X complexes that contain P2X1, P2X2, P2X3 or P2X5 subunits but does not inhibit P2X4 and P2X6 [24, 36]. Variable potencies have been reported for the inhibition of P2X7 by PPADS, with IC50 values ranging from ~100 nM to > 50 μM. However, we can exclude P2X7 here as the relevant receptor because 30 μM BzATP failed to induce luciferase expression despite the fact that rat P2X7 is highly responsive to BzATP in the low μM range . In addition, P2X7 is a low affinity ATP receptor (EC50 > 100 μM ), whereas significant NFAT activation in PC12 cells was already detectable at low micromolar concentrations of ATP. The result that P2X7 does not account for NFAT activation by extracellular ATP in PC12 cells is important because P2X7 mediates NFAT activation in other cell types such as microglia and T cells [38–42]. Finally, the lack of an effect of 30 μM α,β -MeATP excludes the possibility that P2X1 or P2X3 induces the activation of NFAT in PC12 cells because these receptors are activated by submicromolar concentrations of this agonist . Thus, P2X2 and P2X5 remain as the most likely candidates for activating receptors in this pathway.
Based on our data as well as published results, we favour the hypothesis that P2X2 is responsible for the major part of the ATP-induced Ca2+-influx and NFAT activation in our cell model for three reasons: i) the P2X2 receptor was originally cloned from PC12 cells  and appears to be the major P2X isoform in undifferentiated PC12 cells, whereas P2X5 was either reported to be undetectable  or found to be expressed at lower levels (Figure 3A, [15, 44]), ii) calcium influx through P2X2 receptors results in the activation of the MAP kinase cascade , and iii) P2X2 differs from other P2X isoforms, including P2X5, in its slow desensitisation kinetics  and is thus capable of causing sufficient depolarisation of PC12 cells to induce Ca2+ influx via L-type voltage-gated Ca2+ channels [13, 47]. The RT-PCR analysis of the PC12 clone used in the present study confirmed that the cells express both the slowly desensitising P2X2a isoform and the fast desensitising P2X2b splicing variants (Figure 3A).
Thirdly, we showed that, in addition to the dependence on calcineurin activity, maximal NFAT activation also depended on the activation of ERK1/2 (Figure 4). Similarly, MEK1-ERK1/2 signalling augments NFAT transcriptional activity in cultured neonatal cardiomyocytes [48, 49]. As suggested by Sanna et al. , the MEK1-ERK1/2 pathway and the calcineurin-NFAT pathway may converge at the level of the transcription factors. Importantly, Ca2+-induced calcineurin activity alone is not always sufficient to activate NFAT-dependent transcriptional activity, but a second signal that activates the nuclear partner protein of NFAT (NFATn) may be required (Figure 6). AP1, which is a target of ERK1/2, is one of the nuclear partners of NFAT [50, 51]. The simultaneous activation of calcineurin and the MEK-ERK1/2 pathway may explain the very strong ATP-induced NFAT activation that exceeds the effect elicited by the control treatment (PMA and calcimycin).
Finally, we have shown that BTP2 partially inhibited ATP-induced NFAT activation in PC12 cells (Figure 3B), indicating that a BTP2-sensitive Ca2+ influx is necessary for the maximal response under the experimental conditions chosen (incubation time of 3 h). BTP2 is a selective inhibitor of capacitative Ca2+ entry and does not affect Ca2+ handling by mitochondria or endoplasmic reticulum or other Ca2+ channels . NFAT activation in lymphocytes strictly depends on store-operated Ca2+ entry (SOCE), but there exist conflicting data about the contribution of SOCE to Ca2+ responses in PC12 cells [35, 47, 52]. Unfortunately, although several transient receptor potential (TRP) channels have been proposed as targets of BTP2 [28, 53, 54], the primary target of BTP2 is still a matter of debate, and the IC50 values obtained in different experimental systems vary significantly . Therefore, the nature of the BTP2-sensitive mechanism in PC12 cells cannot be deduced from the present experiments, although a TRPC channel may contribute to Ca2+ influx in PC12 cells.
We have found that extracellular ATP upregulates RCAN1-4 mRNA, which is known to be transcribed from an NFAT-regulated promoter [20, 31]. Rcan1 encodes a negative feedback regulator of calcineurin and has multiple effects in neurons, including regulation of vesicle exocytosis, long-term potentiation and facilitation of apoptosis [55–58]. However, NFAT has many more functions in central and peripheral neurons, in particular as a transcription factor that transduces effects of neurotrophins and membrane depolarisation [9, 10, 20, 59, 60]. ATP is well known to change gene expression in neurons and has multiple effects on neurogenesis, neuronal differentiation and neuroprotection [8, 61–63]. Based on the results obtained in neuronal PC12 cells as a model system, we propose that the calcineurin-NFAT pathway is a novel mechanism that mediates the trophic functions of extracellular ATP on neurons.
Our analysis of ATP-induced NFAT activation in PC12 cells has identified a novel pathway through which extracellular ATP can regulate neuronal gene expression and thereby modulate the function and development of neurons. In contrast to immune cells and glia, where P2X7 mediates activation of NFAT, available evidence suggests that, in neurons, P2X2 accounts for this effect. P2X2 produces depolarisation-induced calcium influx through L-type Ca2+ channels and the activation of MEK-ERK1/2 signalling, which are both required for maximal NFAT activation. We propose this pathway as a more general mechanism by which extracellular ATP can exert long-term effects on neuronal cells, which remains to be studied under physiological conditions.
ATP, UTP, 3'-O-(4-benzoyl)benzoyl ATP (BzATP), nifedipine, α,β-MeATP (α,β-methylene-ATP) and PD98059 were obtained from Sigma (Taufkirchen, Germany). Phorbol 12-myristate 13-acetate (PMA), calcimycin (also known as A23187) and FK506 (Tacrolimus) were purchased from Calbiochem (San Diego, CA). Pyridoxal-phosphate-6-azophenyl-2',4'-disulfonate (PPADS) was obtained from Biotrend (Cologne, Germany) and BTP2 (a 3,5-bis(trifluoromethyl)pyrazole derivative, also called YM-58483) was obtained from Cayman Chemical (Ann Arbor, MI). Stock solutions of nifedipine, BTP2, FK506, PD98059 and PMA were prepared in DMSO. ATP, UTP and PPADS were dissolved in water, and calcimycin was dissolved in ethanol.
Cell culture conditions
PC12 cells were grown in high glucose (4.5 g/L) Dulbecco's modified Eagles medium (DMEM) with L-glutamine and sodium pyruvate, supplemented with 5% foetal bovine serum (PAA Laboratories, Pasching, Austria), 10% horse serum (PAA) and 25 mM Hepes buffer (Sigma). Cells were grown to 60-80% confluency in 5% CO2 at 37°C in T75 filter flasks, and cultures were split every 48-72 h without trypsinisation. When indicated, nominal Ca2+ -free conditions were created by adding EGTA to a final concentration of 2 mM. To assess cell viability after ATP treatment, the percentage of stained cells in the trypan blue uptake assay was calculated from hemacytometer counts of at least 300 cells per sample.
Construction of PC12-NFAT-Luc cells
A PC12 subclone with a stably integrated NFAT-dependent luciferase reporter vector was generated in two steps. First, a Flp recombination target (FRT) site was integrated in the genome by transfecting PC12 cells with pFRT/LacZeo (Invitrogen, Carlsbad, CA) and selecting clones resistant to 100 μg/ml Zeocin. Second, the hygromycin resistance gene with a FRT site embedded in the 5' coding region was transferred from pcDNA5/FRT/TO (Invitrogen) into pNFAT-Luc (Stratagene, La Jolla, CA) to generate pNFAT-Luc/FRT. This plasmid was then integrated into the FRT-containing PC12 clone via Flp recombinase-mediated DNA recombination according to the manufacturer's instructions. Luciferase expression from pNFAT-Luc is under the control of four direct repeats of the NFAT binding sequence (GGAGGAAAAACTGTTTCATACAGAAGGCGT) from the IL-2 gene promoter.
Reporter gene assays
For the luciferase assays, PC12-NFAT-Luc cells were plated at 40,000 cells/100 μl/well in a 96-well plate. The following day, the culture medium was completely removed and replaced with medium containing the agents to be tested in the experiment. Antagonists or inhibitors were applied 30 min before stimulation with ATP. After 3 h of incubation, the medium was removed, and the cells were lysed in passive lysis buffer (Promega, Madison, WI) by vigorous shaking for 1 min at room temperature. Luciferase activity was determined by mixing an aliquot of the lysate with 4 vol of the luciferase assay mix according to Gaunitz and Papke  and measuring light emission in an Orion Microplate Luminometer (Berthold Detection Systems, Pforzheim, Germany). All data were obtained from triplicate wells.
Whole cell lysates for immunodetection of ERK phosphorylation were prepared by collecting the cells by centrifugation in a micro test tube and subsequent lysis with 100 μl/well (in a 6-well plate) boiling SDS-buffer (20 mM Tris, pH 7.4; 1% SDS). The phosphorylation of ERK1/2 was assessed by Western blot analysis using polyclonal rabbit antibodies specific for phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) and total p44/42 MAPK (Cell Signaling Technology, Danvers, MA). The Western blots were developed using horseradish peroxidase-coupled secondary antibodies and chemiluminescence detection. Signal intensities were quantitated with a LAS-3000 CCD imaging system and the AIDA Image Analyzer 5.0 program (Raytest, Straubenhardt, Germany).
To quantify the mRNA levels of RCAN1 and BDNF, 3x106 PC12-NFAT-Luc cells were plated in 60 mm culture dishes. The next day, the medium was changed, and ATP and FK506 were added. The cells were incubated with ATP for 3 h, while FK506 was added 30 min before stimulation with ATP. The RNeasy Mini Kit (Qiagen, Hilden, Germany) was used for RNA purification according to the manual. For the cDNA synthesis, 1 μg of total RNA was reverse transcribed using 1 μg of oligo(dT) and MMLV reverse transcriptase (Promega) at 40°C for 1 h. The resulting cDNAs were analysed using a LightCycler 480 system and SYBR Green master mix reagent (Roche Applied Science), using the following PCR conditions: 5 min initial denaturation at 95°C, followed by 45 cycles of 10 s at 95°C, 10 s at 50-62°C, 15 s at 72°C and 1 s at 74°C. The sequences of the oligonucleotide primers used for the specific detection of the rat RCAN1-4 transcript [GenBank NM_153724.2] and the exon IV-containing Bdnf transcript [GenBank EF125679.1] are given in the Supplementary material (Additional file 1). The beta-2 microglobulin gene was used as a housekeeping gene for normalization.
The sequences of the primers used for the amplification of the P2X and NFAT sequences are given in the Supplementary material (additional file 1). The REDTaq PCR Reaction Mix (Sigma) was used under the following PCR conditions: 2 min initial denaturation at 94°C, followed by 35 cycles of 30 s at 94°C, 30 s at 52-58°C and 1 min at 72°C. The positive control plasmid for amplification of P2X7 cDNA was kindly provided by Günther Schmalzing in our Institute.
The GraphPad Prism 5.0 program (GraphPad Software, La Jolla, CA) was used for curve fitting by nonlinear regression and statistical analysis. Results were tested for statistical significance by ANOVA and Bonferroni's correction for multiple comparisons.
β-MeATP: α,β-methylene ATP
brain-derived neurotrophic factor
nuclear factor of activated T cells
phorbol 12-myristate 13-acetate
regulator of calcineurin
reverse transcription polymerase chain reaction
store-operated Ca2+ entry.
We wish to acknowledge the excellent technical assistance of Simone Bamberg-Lemper who generated the PC12-NFAT-Luc cell line. We are very grateful to Günther Schmalzing and Ralf Hausmann for providing reagents and valuable advice. GS thanks the Alexander von Humboldt Foundation for a postdoctoral research fellowship. This work was partially supported by the Deutsche Forschungsgemeinschaft (Be 1967/2-1).
- Burnstock G: Purinergic signalling and disorders of the central nervous system. Nat Rev Drug Discov. 2008, 7: 575-590. 10.1038/nrd2605.View ArticlePubMed
- Abbracchio MP, Burnstock G, Verkhratsky A, Zimmermann H: Purinergic signalling in the nervous system: an overview. Trends Neurosci. 2009, 32: 19-29. 10.1016/j.tins.2008.10.001.View ArticlePubMed
- Fields D, Burnstock G: Purinergic signalling in neuron-glial interactions. Nature Rev Neurosci. 2006, 7: 423-436. 10.1038/nrn1928.View Article
- Ralevic V, Burnstock G: Receptors for purines and pyrimidines. Pharmacol Rev. 1998, 50: 413-492.PubMed
- Rodrigues RJ, Almeida T, Richardson PJ, Oliveira CR, Cunha RA: Dual presynaptic control by ATP of glutamate release via facilitatory P2X1, P2X2/3, and P2X3 and inhibitory P2Y1, P2Y2, and/or P2Y4 receptors in the rat hippocampus. J Neurosci. 2005, 25: 6286-6295. 10.1523/JNEUROSCI.0628-05.2005.View ArticlePubMed
- Shigetomi E, Kato F: Action potential-independent release of glutamate by Ca2+ entry through presynaptic P2X receptors elicits postsynaptic firing in the brainstem autonomic network. J Neurosci. 2004, 24: 3125-3135. 10.1523/JNEUROSCI.0090-04.2004.View ArticlePubMed
- Stojilkovic SS: Purinergic regulation of hypothalamopituitary functions. Trends Endocrinol Metab. 2009, 20: 460-468. 10.1016/j.tem.2009.05.005.PubMed CentralView ArticlePubMed
- Neary JT, Zimmermann H: Trophic functions of nucleotides in the central nervous system. Trends Neurosci. 2009, 32: 189-198. 10.1016/j.tins.2009.01.002.View ArticlePubMed
- Graef IA, Mermelstein PG, Stankunas K, Neilson JR, Deisseroth K, Tsien RW, Crabtree GR: L-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons. Nature. 1999, 401: 703-708. 10.1038/44378.View ArticlePubMed
- Graef IA, Wang F, Charron F, Chen L, Neilson J, Tessier-Lavigne M, Crabtree GR: Neurotrophins and netrins require calcineurin/NFAT signaling to stimulate outgrowth of embryonic axons. Cell. 2003, 113: 657-670. 10.1016/S0092-8674(03)00390-8.View ArticlePubMed
- Nguyen T, Di Giovanni S: NFAT signaling in neural development and axon growth. Int J Dev Neurosci. 2008, 26: 141-145. 10.1016/j.ijdevneu.2007.10.004.PubMed CentralView ArticlePubMed
- Arslan G, Filipeanu CM, Irenius E, Kull B, Clementi E, Allgaier C, Erlinge D, Fredholm BB: P2Y receptors contribute to ATP induced increases in intracellular calcium in differentiated but not undifferentiated PC12 cells. Neuropharmacology. 2000, 39: 482-496. 10.1016/S0028-3908(99)00141-0.View ArticlePubMed
- Hur EM, Park TJ, Kim KT: Coupling of L-type voltage-sensitive calcium channels to P2X(2) purinoceptors in PC-12 cells. Am J Physiol Cell Physiol. 2001, 280: C1121-1129.PubMed
- Arthur DB, Akassoglou K, Insel PA: P2Y2 receptor activates nerve growth factor/TrkA signaling to enhance neuronal differentiation. Proc Natl Acad Sci USA. 2005, 102: 19138-19143. 10.1073/pnas.0505913102.PubMed CentralView ArticlePubMed
- Arthur DB, Taupenot L, Insel PA: Nerve growth factor-stimulated neuronal differentiation induces changes in P2 receptor expression and nucleotide-stimulated catecholamine release. J Neurochem. 2007, 100: 1257-1264. 10.1111/j.1471-4159.2006.04305.x.View ArticlePubMed
- Sato A, Arimura Y, Manago Y, Nishikawa K, Aoki K, Wada E, Suzuki Y, Osaka H, Setsuie R, Sakurai M, Amano T, Aoki S, Wada K, Noda M: Parkin potentiates ATP-induced currents due to activation of P2X receptors in PC12 cells. J Cell Physiol. 2006, 209: 172-182. 10.1002/jcp.20719.View ArticlePubMed
- Belliveau DJ, Bani-Yaghoub M, McGirr B, Naus CC, Rushlow WJ: Enhanced neurite outgrowth in PC12 cells mediated by connexin hemichannels and ATP. J Biol Chem. 2006, 281: 20920-20931. 10.1074/jbc.M600026200.View ArticlePubMed
- Homma K, Niino Y, Hotta K, Oka K: Ca(2+) influx through P2X receptors induces actin cytoskeleton reorganization by the formation of cofilin rods in neurites. Mol Cell Neurosci. 2008, 37: 261-270. 10.1016/j.mcn.2007.10.001.View ArticlePubMed
- Ho AM, Jain J, Rao A, Hogan PG: Expression of the transcription factor NFATp in a neuronal cell line and in the murine nervous system. J Biol Chem. 1994, 269: 28181-28186.PubMed
- Cano E, Canellada A, Minami T, Iglesias T, Redondo JM: Depolarization of neural cells induces transcription of the Down syndrome critical region 1 isoform 4 via a calcineurin/nuclear factor of activated T cells-dependent pathway. J Biol Chem. 2005, 280: 29435-29443. 10.1074/jbc.M506205200.View ArticlePubMed
- Nguyen T, Lindner R, Tedeschi A, Forsberg K, Green A, Wuttke A, Gaub P, Di Giovanni S: NFAT-3 is a transcriptional repressor of the growth-associated protein 43 during neuronal maturation. J Biol Chem. 2009, 284: 18816-18823. 10.1074/jbc.M109.015719.PubMed CentralView ArticlePubMed
- Vollmayer P, Koch M, Braun N, Heine P, Servos J, Israr E, Kegel B, Zimmermann H: Multiple ecto-nucleotidases in PC12 cells: identification and cellular distribution after heterologous expression. J Neurochem. 2001, 78: 1019-1028. 10.1046/j.1471-4159.2001.00480.x.View ArticlePubMed
- Fiering S, Northrop JP, Nolan GP, Mattila PS, Crabtree GR, Herzenberg LA: Single cell assay of a transcription factor reveals a threshold in transcription activated by signals emanating from the T-cell antigen receptor. Genes Dev. 1990, 4: 1823-1834. 10.1101/gad.4.10.1823.View ArticlePubMed
- Jarvis MF, Khakh BS: ATP-gated P2X cation-channels. Neuropharmacology. 2009, 56: 208-215. 10.1016/j.neuropharm.2008.06.067.View ArticlePubMed
- Brändle U, Spielmanns P, Osteroth R, Sim J, Surprenant A, Buell G, Ruppersberg JP, Plinkert PK, Zenner HP, Glowatzki E: Desensitization of the P2X(2) receptor controlled by alternative splicing. FEBS Lett. 1997, 404: 294-298. 10.1016/S0014-5793(97)00128-2.View ArticlePubMed
- Ishikawa J, Ohga K, Yoshino T, Takezawa R, Ichikawa A, Kubota H, Yamada T: A pyrazole derivative, YM-58483, potently inhibits store-operated sustained Ca2+ influx and IL-2 production in T lymphocytes. J Immunol. 2003, 170: 4441-4449.View ArticlePubMed
- Kinoshita H, Kuwahara K, Nishida M, Jian Z, Rong X, Kiyonaka S, Kuwabara Y, Kurose H, Inoue R, Mori Y, Li Y, Nakagawa Y, Usami S, Fujiwara M, Yamada Y, Minami T, Ueshima K, Nakao K: Inhibition of TRPC6 channel activity contributes to the antihypertrophic effects of natriuretic peptides-guanylyl cyclase-A signaling in the heart. Circ Res. 2010, 106: 1849-1860. 10.1161/CIRCRESAHA.109.208314.View ArticlePubMed
- Kiyonaka S, Kato K, Nishida M, Mio K, Numaga T, Sawaguchi Y, Yoshida T, Wakamori M, Mori E, Numata T, Ishii M, Takemoto H, Ojida A, Watanabe K, Uemura A, Kurose H, Morii T, Kobayashi T, Sato Y, Sato C, Hamachi I, Mori Y: Selective and direct inhibition of TRPC3 channels underlies biological activities of a pyrazole compound. Proc Natl Acad Sci USA. 2009, 106: 5400-5405. 10.1073/pnas.0808793106.PubMed CentralView ArticlePubMed
- Parekh AB: Store-operated CRAC channels: function in health and disease. Nat Rev Drug Discov. 2010, 9: 399-410. 10.1038/nrd3136.View ArticlePubMed
- Davies KJ, Ermak G, Rothermel BA, Pritchard M, Heitman J, Ahnn J, Henrique-Silva F, Crawford D, Canaider S, Strippoli P, Carinci P, Min KT, Fox DS, Cunningham KW, Bassel-Duby R, Olson EN, Zhang Z, Williams RS, Gerber HP, Pérez-Riba M, Seo H, Cao X, Klee CB, Redondo JM, Maltais LJ, Bruford EA, Povey S, Molkentin JD, McKeon FD, Duh EJ, Crabtree GR, Cyert MS, de la Luna S, Estivill X: Renaming the DSCR1/Adapt78 gene family as RCAN: regulators of calcineurin. FASEB J. 2007, 21: 3023-3028. 10.1096/fj.06-7246com.View ArticlePubMed
- Canellada A, Ramirez BG, Minami T, Redondo JM, Cano E: Calcium/calcineurin signaling in primary cortical astrocyte cultures: Rcan1-4 and cyclooxygenase-2 as NFAT target genes. Glia. 2008, 56: 709-722. 10.1002/glia.20647.View ArticlePubMed
- Aid T, Kazantseva A, Piirsoo M, Palm K, Timmusk T: Mouse and rat BDNF gene structure and expression revisited. J Neurosci Res. 2007, 85: 525-535. 10.1002/jnr.21139.PubMed CentralView ArticlePubMed
- Hong EJ, McCord AE, Greenberg ME: A biological function for the neuronal activity-dependent component of Bdnf transcription in the development of cortical inhibition. Neuron. 2008, 60: 610-24. 10.1016/j.neuron.2008.09.024.PubMed CentralView ArticlePubMed
- Vashishta A, Habas A, Pruunsild P, Zheng JJ, Timmusk T, Hetman M: Nuclear factor of activated T-cells isoform c4 (NFATc4/NFAT3) as a mediator of antiapoptotic transcription in NMDA receptor-stimulated cortical neurons. J Neurosci. 2009, 29: 15331-15340. 10.1523/JNEUROSCI.4873-09.2009.PubMed CentralView ArticlePubMed
- Barry VA, Cheek TR: Extracellular ATP triggers two functionally distinct calcium signalling pathways in PC12 cells. J Cell Sci. 1994, 107: 451-462.PubMed
- Gever JR, Cockayne DA, Dillon MP, Burnstock G, Ford AP: Pharmacology of P2X channels. Pflügers Arch. 2006, 452: 513-537. 10.1007/s00424-006-0070-9.View ArticlePubMed
- Hibell AD, Kidd EJ, Chessell IP, Humphrey PP, Michel AD: Apparent species differences in the kinetic properties of P2X(7) receptors. Br J Pharmacol. 2000, 130: 167-173. 10.1038/sj.bjp.0703302.PubMed CentralView ArticlePubMed
- Ferrari D, Stroh C, Schulze-Osthoff K: P2X7/P2Z purinoreceptor-mediated activation of transcription factor NFAT in microglial cells. J Biol Chem. 1999, 274: 13205-13210. 10.1074/jbc.274.19.13205.View ArticlePubMed
- Adinolfi E, Cirillo M, Woltersdorf R, Falzoni S, Chiozzi P, Pellegatti P, Callegari MG, Sandonà D, Markwardt F, Schmalzing G, Di Virgilio F: Trophic activity of a naturally occurring truncated isoform of the P2X7 receptor. FASEB J. 2010, 24: 3393-3404. 10.1096/fj.09-153601.View ArticlePubMed
- Shiratori M, Tozaki-Saitoh H, Yoshitake M, Tsuda M, Inoue K: P2X7 receptor activation induces CXCL2 production in microglia through NFAT and PKC/MAPK pathways. J Neurochem. 2010, 114: 810-819. 10.1111/j.1471-4159.2010.06809.x.View ArticlePubMed
- Yip L, Woehrle T, Corriden R, Hirsh M, Chen Y, Inoue Y, Ferrari V, Insel PA, Junger WG: Autocrine regulation of T-cell activation by ATP release and P2X7 receptors. FASEB J. 2009, 23: 1685-1693. 10.1096/fj.08-126458.PubMed CentralView ArticlePubMed
- Kataoka A, Tozaki-Saitoh H, Koga Y, Tsuda M, Inoue K: Activation of P2X7 receptors induces CCL3 production in microglial cells through transcription factor NFAT. J Neurochem. 2009, 108: 115-125.View ArticlePubMed
- Brake AJ, Wagenbach MJ, Julius D: New structural motif for ligand-gated ion channels defined by an ionotropic ATP receptor. Nature. 1994, 371: 519-523. 10.1038/371519a0.View ArticlePubMed
- Sun JH, Cai GJ, Xiang ZH: Expression of P2X purinoceptors in PC12 phaeochromocytoma cells. Clin Exp Pharmacol Physiol. 2007, 34: 1282-1286. 10.1111/j.1440-1681.2007.04718.x.View ArticlePubMed
- Swanson KD, Reigh C, Landreth GE: ATP-stimulated activation of the mitogen-activated protein kinases through ionotrophic P2X2 purinoreceptors in PC12 cells. Difference in purinoreceptor sensitivity in two PC12 cell lines. J Biol Chem. 1998, 273: 19965-19971. 10.1074/jbc.273.32.19965.View ArticlePubMed
- North RA: Molecular physiology of P2X receptors. Physiol Rev. 2002, 82: 1013-1067.View ArticlePubMed
- Ariano P, Zamburlin P, D'Alessandro R, Meldolesi J, Lovisolo D: Differential repression by the transcription factor REST/NRSF of the various Ca2+ signalling mechanisms in pheochromocytoma PC12 cells. Cell Calcium. 2010, 47: 360-368. 10.1016/j.ceca.2010.01.007.View ArticlePubMed
- Sanna B, Bueno OF, Dai YS, Wilkins BJ, Molkentin JD: Direct and indirect interactions between calcineurin-NFAT and MEK1-extracellular signal-regulated kinase 1/2 signaling pathways regulate cardiac gene expression and cellular growth. Mol Cell Biol. 2005, 25: 865-878. 10.1128/MCB.25.3.865-878.2005.PubMed CentralView ArticlePubMed
- Ichida M, Finkel T: Ras regulates NFAT3 activity in cardiac myocytes. J Biol Chem. 2001, 276: 3524-3530. 10.1074/jbc.M004275200.View ArticlePubMed
- Wu H, Peissley A, Graef IA, Crabtree GR: NFAT signaling and the invention of vertebrates. Trends Cell Biol. 2007, 17: 251-260. 10.1016/j.tcb.2007.04.006.View ArticlePubMed
- Müller MR, Rao A: NFAT, immunity and cancer: a transcription factor comes of age. Nat Rev Immunol. 2010, 10: 645-656. 10.1038/nri2818.View ArticlePubMed
- Taylor SC, Peers C: Three distinct Ca2+ influx pathways couple acetylcholine receptor activation to catecholamine secretion from PC12 cells. J Neurochem. 2000, 75: 1583-1589.View ArticlePubMed
- He LP, Hewavitharana T, Soboloff J, Spassova MA, Gill DL: A functional link between store-operated and TRPC channels revealed by the 3,5-bis(trifluoromethyl)pyrazole derivative, BTP2. J Biol Chem. 2005, 280: 10997-11006. 10.1074/jbc.M411797200.View ArticlePubMed
- Takezawa R, Cheng H, Beck A, Ishikawa J, Launay P, Kubota H, Kinet JP, Fleig A, Yamada T, Penner R: A pyrazole derivative potently inhibits lymphocyte Ca2+ influx and cytokine production by facilitating transient receptor potential melastatin 4 channel activity. Mol Pharmacol. 2006, 69: 1413-1420. 10.1124/mol.105.021154.View ArticlePubMed
- Ermak G, Harris CD, Davies KJ: The DSCR1 (Adapt78) isoform 1 protein calcipressin 1 inhibits calcineurin and protects against acute calcium-mediated stress damage, including transient oxidative stress. FASEB J. 2002, 16: 814-824. 10.1096/fj.01-0846com.View ArticlePubMed
- Keating DJ, Dubach D, Zanin MP, Yu Y, Martin K, Zhao YF, Chen C, Porta S, Arbonés ML, Mittaz L, Pritchard MA: DSCR1/RCAN1 regulates vesicle exocytosis and fusion pore kinetics: implications for Down syndrome and Alzheimer's disease. Hum Mol Genet. 2008, 17: 1020-1030.View ArticlePubMed
- Hoeffer CA, Dey A, Sachan N, Wong H, Patterson RJ, Shelton JM, Richardson JA, Klann E, Rothermel BA: The Down syndrome critical region protein RCAN1 regulates long-term potentiation and memory via inhibition of phosphatase signaling. J Neurosci. 2007, 27: 13161-13172. 10.1523/JNEUROSCI.3974-07.2007.View ArticlePubMed
- Sun X, Wu Y, Chen B, Zhang Z, Zhou W, Tong Y, Yuan J, Xia K, Gronemeyer H, Flavell RA, Song W: Regulator of calcineurin 1 (RCAN1) facilitates neuronal apoptosis through caspase-3 activation. J Biol Chem. 2011, 286: 9049-9062. 10.1074/jbc.M110.177519.PubMed CentralView ArticlePubMed
- Groth RD, Coicou LG, Mermelstein PG, Seybold VS: Neurotrophin activation of NFAT-dependent transcription contributes to the regulation of pro-nociceptive genes. J Neurochem. 2007, 102: 1162-1174. 10.1111/j.1471-4159.2007.04632.x.View ArticlePubMed
- Jung H, Miller RJ: Activation of the nuclear factor of activated T-cells (NFAT) mediates upregulation of CCR2 chemokine receptors in dorsal root ganglion (DRG) neurons: a possible mechanism for activity-dependent transcription in DRG neurons in association with neuropathic pain. Mol Cell Neurosci. 2008, 37: 170-177.PubMed CentralView ArticlePubMed
- Burnstock G, Verkhratsky A: Long-term (trophic) purinergic signalling: purinoceptors control cell proliferation, differentiation and death. Cell Death Dis. 2010, 1: e9-10.1038/cddis.2009.11.PubMed CentralView ArticlePubMed
- Majumder P, Trujillo CA, Lopes CG, Resende RR, Gomes KN, Yuahasi KK, Britto LR, Ulrich H: New insights into purinergic receptor signaling in neuronal differentiation, neuroprotection, and brain disorders. Purinergic Signal. 2007, 3: 317-331. 10.1007/s11302-007-9074-y.PubMed CentralView ArticlePubMed
- Dale N: Dynamic ATP signalling and neural development. J Physiol. 2008, 586: 2429-2436. 10.1113/jphysiol.2008.152207.PubMed CentralView ArticlePubMed
- Gaunitz F, Papke M: Gene transfer and expression. Methods Mol Biol. 1998, 107: 361-370.PubMed
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