Adult (4 months of age) female Wistar rats obtained from Harlan Laboratories (Madison, Wisconsin) were housed in pairs in a pathogen-free vivarium under controlled condition (temperature 22 ± 1°C and humidity 70 ± 5%) and a 14:10 hour light:dark cycle was maintained. All animals were housed in the same room so that temperature, humidity, and lighting conditions are similar for all groups. Animals had free access to food (regular rat chow) and water delivered through an automated and filtered system. Animals were also handled daily throughout the study so that they could get acclimated to the research personnel thereby decreasing stress. Experiments (see Study Design) started two weeks after arrival of the animals and all experimental protocols in this study were approved by the Institutional Animal Care and Use Committee and in accordance with the National Institutes of Health guidelines.
Continuous activity monitoring was performed using a microtelemetry device (Data Science International, St. Paul, MN) to evaluate presence of fatigue due to chemotherapy. The device was implanted via laparotomy through a 1 cm incision in briefly anesthetized rats (gas anesthesia of 2.5% isofluorane and 30% oxygen mixture delivered through a cone). After telemetry implantation, rats were monitored every 10 minutes until fully awake then daily for presence of pain; if pain was observed (demonstrated as continuous scratching) then topical lidocaine was applied. The individual receiver boards that contain an infrared motion sensor placed under the floor of each cage continuously monitored general activity (frequency [Hz]). These data were fed into a peripheral processor connected to a computer, where they registered as activity counts and stored every 5 minutes. Activity counts were generated by any locomotion in the cage as detected by the infrared sensor from the implanted telemetry. The individual telemetry devices were calibrated before implantation and baseline activity level was collected for one week before the start of the chemotherapy protocol.
Two weeks after recovery from minimitter implantation, rats were randomly assigned to either chemotherapy (n = 12) or saline control (n = 12) group. Rats in the chemotherapy group received the drug combination of cyclophosphamide (40 mg/Kg; Sigma-Aldrich, St. Louis, MO), methotrexate (37.5 mg/Kg; Wyeth Ayerst, Itasca, IL), and 5-fluorouracil (75 mg/Kg; Sigma-Aldrich, St. Louis, MO) dissolved in normal saline. Rats in the control group received normal saline of equal volume to control for the effects of stress induced by the injection. The dosages selected were based on our preliminary work, which showed that animals tolerated these doses with minimal weight loss or death. Both CMF and normal saline injections were given intraperitoneally once a week for a total of 4 weeks and rats were weighed every other day during the chemotherapeutic regimen. Rats were also monitored daily for other possible toxicity effects of chemotherapy (n = 0) such as apathy, excessive grooming, motor impairment, hair loss, and diarrhea.
Two weeks after the final CMF or saline injections, rats were tested in the water maze to evaluate cognitive impairment. The delay in behavioral testing was done to allow the animals to recover from the drug-induced fatigue that may confound the behavioral tests results. Both hippocampal and non-hippocampal learning and memory processes, were assessed. All testing were done approximately 2 hours prior to the onset of the dark cycle to ensure that it is close to the rats' active period.
Spatial learning and memory (acquisition and recall), tasks sensitive to hippocampal dysfunction were examined using the water maze task. The water maze apparatus consisted of a circular tub made of galvanized steel measuring 1.52 m in diameter; and the interior surface was painted white. The use of a large tub decreased the probability that the rats will find the goal/platform by chance. During testing, the tub was filled with tepid water (22 ± 2°C) and made opaque by the addition of powdered milk. An inverted white flowerpot, submerged 2 cm beneath the water's surface served as the goal/platform and the opaqueness of the water enabled the goal/platform to be concealed. Extramaze cues, such as overhead lighting, windows and room noise were held constant during testing. The pool was divided into four quadrants of equal surface area and the starting locations for testing were assigned north, south, east, and west. The goal/platform was located in the middle of the southeast quadrant approximately 22 cm from the pool rim. The day before actual testing started, rats were allowed a habituation swim for 10 seconds without the platform. The habituation swim and consistent water temperature throughout the test days were necessary to minimize animal stress during water maze testing. Animals received four trials a day for four consecutive days. A different starting point was used on each of the four daily trials and the order of starting points was random. If the rat failed to find the hidden platform within 3 minutes, they were guided to the platform and given a swim latency score of 180 seconds. The animals were allowed to stay on the platform for 20 seconds then towel-dried until the next trial. A minimum of two minutes was used between trials to provide a rest period for the animals and avoid "practice effect." During the trials, swim latency (time to reach the platform) and the path taken by the animals to reach the platform were recorded by a video camera connected to an image analyzer (Water Maze System Version 4.20, Columbus, OH) and these data were used to assess performance in the water maze task. In addition, swimming speed (path length/swim latency) was used to assess the motoric activity of the rats in performing the task. Black shoe polish was applied on top of the animals' head to facilitate video camera tracking as rats swim in the water maze. On the fifth day, a probe trial was performed wherein the goal/platform was removed from the pool. In addition, rats were tested in a cued trial for one day (4 trials) following the water maze task where they were allowed to swim in the tub to locate a visible pole attached to the goal. The cued trial was performed to control for potential visual problems that may influence performance in the water maze.
The rats also received discrimination-learning test after two days of rest following the cued trials. The discrimination-learning task is sensitive to dysfunction involving the striatum. In this task the rats had to discriminate between black and white visible goals to find the hidden platform and all extra-maze cues in the room were covered. The goal painted white was placed on top of the hidden platform to provide escape (P+) from the water (located in the southeast quadrant); whereas the other one painted black was floating (P-) and not able to offer sufficient buoyancy to support the rat (located in the southwest quadrant). Both visible goals were placed 8 cm above the water level. For this task, the additional measure obtained was the number of correct choices of P+ compared to P- since the aim was to train the rats to avoid P-.
The thymidine analog Bromodeoxyuridine (BrdU; Chemicon, Temecula, CA) was used to label proliferating cells. BrdU incorporates into the genetic materials on mitotic division within 2 hours after injection, after which it can be detected immunohistochemically in the daughter cells . BrdU was dissolved in 0.9% sterile NaCl and filtered at 22 μm. The resulting solution was injected at 100 mg/kg intraperitoneally in all rat groups. Injections were given 4 hours prior to euthanasia.
All rats were euthanized using CO2 inhalation, the brains removed, cut in half sagitally and immediately placed in liquid nitrogen until processed. Half of the brain was used for immunohistochemistry (detection of cell proliferation and histone acetylation) while the other half was used for determination of histone deacetylase (HDAC) activity. The half used for immunohistochemistry was fixed in 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.3) overnight then cryoprotected before sectioning.
The fixed brains were sectioned at 30 μm thickness using a cryostat. Tissue sections were obtained covering the entire hippocampal region in its rostro-caudal extension and the free-floating section method was used for immunohistochemistry to examine histone modifications and hippocampal cell proliferation. For detection of BrdU-labeled nuclei, DNA was denatured to expose the antigen before incubation in anti-BrdU primary antibody. Briefly, free-floating sections were pretreated in 50% formamide/50% 2xsaline-sodium citrate buffer (SSC) at 65°C for 2 h, rinsed in 2xSSC, and then incubated in 2 N HCl at 37°C for 30 min. Tissues were then rinsed in borate buffer (pH 8.5) for 15 minutes and placed in 0.6% H2O2 in Tris-buffered saline (TBS) for 30 minutes to block endogenous peroxidase, followed by several rinses in TBS (pH 7.5). Tissues were then placed in TBS/0.1% Triton X-100/3% donkey serum (TBS-TS) for 1 hour followed by incubation with anti-BrdU primary antibodies at a concentration of 1:400 (monoclonal mouse; Boehringer Mannheim; Indianapolis, IN) in TBS-TS overnight at 4°C. The following day, the primary antibody was detected using biotinylated immunoglobulin G (IgG) donkey anti-mouse secondary antibodies (Vector Laboratories; Burlingame, CA) at a concentration of 1:200 for 2 hours. Tissues were then rinsed in TBS and incubated in avidin-biotin complex (ABC kit; Vector Laboratories) for 1 hour at room temperature. Immunoreactions were visualized by treatment of section with hydrogen peroxide and 3,3'- diaminobenzidine tetrahydrochloride in Tris buffer (pH 7.3). After thorough rinsing, the tissue sections were mounted on gelatin-coated slides and dried, and coverslips were applied. To minimize intergroup and interbrain staining variability and to ensure reproducibility of results, tissues from all experimental groups were run simultaneously and under identical conditions.
The total number of BrdU-positive cells in the granule cell layer and its corresponding sample volume were determined in 8 coronal sections, 240 μm apart, using the optical disector method (StereoInvestigator, MicroBrightfield, Colchester, VT). Briefly, each section was examined at a magnification of 40x, and an unbiased counting frame was positioned randomly across the dentate gyrus area. The 1st focal plane (i.e., the top of the tissue section where cells came into focus) was identified, and cells in this field of view were disregarded. A focal plane (approximately 3 μm apart) was then gradually passed through each section by adjusting the focus of the microscope slowly, and the labeled cells encountered while focusing through the section were counted. The number of labeled cells was related to the number of sections counted and was multiplied by the reference volume to provide an unbiased estimation of the total number of BrdU-positive cells. Reference volume in the dentate gyrus was obtained using the Cavalieri principle, wherein the granule cells were counted at random systematic sampling points superimposed onto the image projected on the computer. The reference volume was the product of the sum of the number of points that fell within the boundaries of the granular layer and the mean post- processing thickness of Nissl-stained sections. The section thickness of 30 μm (microtome setting) was used because it was assumed that the net error by using the whole-section thickness for the volume was smaller than the error introduced by measuring the postprocessing section thickness on each slide and counting in a fixed fraction of it.
For detection of histone acetylation, tissues were first sequentially treated with 0.3% hydrogen peroxide in PBS for 30 minutes then rinsed with 0.1 M phosphate buffered saline (pH 7.3) and placed in the blocking solution of 3% serum, 0.1% Triton-X, and 1% bovine serum albumin for 1 hour. After blocking, tissues were washed in phosphate buffered saline (PBS) followed by incubation for 24 hours at 4°C in rabbit anti-acetyl-H3 (1:1000; Upstate Cell Signaling, Bellirica, MA) with gentle agitation. Anti-acetyl-H3 recognizes histone acetylation at the Lys9 and Lys14 residues. The primary antibody was detected using preadsorbed biotinylated IgG secondary antibodies (1:200, Vector Laboratories, Burlingame, CA) for 1 hour at room temperature. The tissues were then washed and incubated in avidin-biotin complex (ABC kit, Vector Laboratories, Burlingame, CA) for one hour at room temperature. Immunoreactions were visualized by treatment of tissue sections with hydrogen peroxide and 3,3'-diaminobenzidine tetrahydrochloride (DAB) in Tris buffer (pH 7.3). After thorough rinsing, the tissue sections were mounted on gelatin-coated slides, dried, and coverslipped. Tissues from all experimental groups were run simultaneously and under identical conditions to ensure reproducibility of results. In addition, a pre-dilution test was done to ensure specificity of the antibody and negative controls, involving deletion of the primary antibody, were used to rule out any nonspecific interactions. Quantification of histone H3 changes in the hippocampus, striatum, and prefrontal cortex was determined by the surface area covered by anti-acetyl-H3 immunoreactivity using an area-fractionator grid defined by the StereoInvestigator (MicroBrightfield, Colchester, VT) computerized analysis system as previously described .
Histone Deacetylase (HDAC) Activity
To determine histone deacetylation, HDAC activity was measured from the total cell lysate after whole hippocampal tissues were homogenized using a nuclear extraction kit (Sigma, St. Louis, MO). Total HDAC activity (class I and II HDACs) was determined according to the manufacturer's instructions for the colorimetric HDAC activity assay kit (BioVision Research, Mountain View, CA) by measuring the deacetylation of acetylated lysine side chains. The optical density (OD) of the samples was measured using an ELISA plate reader at 405 nm (Spectra MR; Dynex Technologies, Chantilly, VA). The results were calculated as OD per milligram of protein and then converted to percentage of control.
The SAS general linear model (SAS Institute, North Carolina) procedures for one-way analysis of variance (ANOVA) were used to examine effects of experimental conditions (chemotherapy vs. saline groups) on epigenetic modifications (histone acetylation and HDAC activity). Repeated measures ANOVA were used to examine chemotherapy effects on weight loss, activity, and behavioral performance to determine differences in latency, path length, swimming speed, exploration time, and discrimination ratio. All error bars represent ± standard error of the mean (SEM) of the sample size used in the study.