The TRPC2 channel forms protein-protein interactions with Homer and RTP in the rat vomeronasal organ
© Mast et al; licensee BioMed Central Ltd. 2010
Received: 11 October 2009
Accepted: 21 May 2010
Published: 21 May 2010
The signal transduction cascade operational in the vomeronasal organ (VNO) of the olfactory system detects odorants important for prey localization, mating, and social recognition. While the protein machinery transducing these external cues has been individually well characterized, little attention has been paid to the role of protein-protein interactions among these molecules. Development of an in vitro expression system for the transient receptor potential 2 channel (TRPC2), which establishes the first electrical signal in the pheromone transduction pathway, led to the discovery of two protein partners that couple with the channel in the native VNO.
Homer family proteins were expressed in both male and female adult VNO, particularly Homer 1b/c and Homer 3. In addition to this family of scaffolding proteins, the chaperones receptor transporting protein 1 (RTP1) and receptor expression enhancing protein 1 (REEP1) were also expressed. RTP1 was localized broadly across the VNO sensory epithelium, goblet cells, and the soft palate. Both Homer and RTP1 formed protein-protein interactions with TRPC2 in native reciprocal pull-down assays and RTP1 increased surface expression of TRPC2 in in vitro assays. The RTP1-dependent TRPC2 surface expression was paralleled with an increase in ATP-stimulated whole-cell current in an in vitro patch-clamp electrophysiological assay.
TRPC2 expression and channel activity is regulated by chaperone- and scaffolding-associated proteins, which could modulate the transduction of chemosignals. The developed in vitro expression system, as described here, will be advantageous for detailed investigations into TRPC2 channel activity and cell signalling, for a channel protein that was traditionally difficult to physiologically assess.
The mammalian accessory olfactory system (AOS) functions as a detector for chemical signals (chemosignals) concerning social organization and conspecific reproductive status [1, 2]. The vomeronasal organ (VNO) is the primary sensory organ for the AOS. In rodents, the VNO is an encapsulated neuroepithelium containing a lumen and is able to aspirate fluids via a vascular pump [3, 4]. Vomeronasal sensory neurons (VSN) express vomeronasal receptors (VRs) belonging to one of the two families of G-protein coupled receptors (GPCRs) specific to the VNO, the V1Rs and V2Rs [5–7]. Upon binding of a chemosignal, V1Rs and V2Rs activate the G-proteins, Gαi2 and Gαo, respectively . G-protein activation can ultimately result in a non-specific cation current through the canonical transient receptor potential channel type 2 (TRPC2) [8–10].
VNO function is dependent upon TRPC2 [11–13]. Adaptor proteins scaffold TRPC to proteins such as inositol 1,4,5-trisphosphate receptor type 3 (IP3R3) [14–16]. In the invertebrate retina TRP is in a scaffold-mediated complex whereby deletion of the scaffold leads to complex degradation and altered light responses . The TRP channel and IP3 receptor are co-localized in VSN microvilli  whereby peptide disruption of the protein-protein interaction between them inhibits chemosignal-induced currents .
Homers are adaptor proteins that bind to proline-rich sequences on proteins associated with calcium signalling . Long Homer isoforms (1b/c, 2a/b, 3) contain, whereby the immediate-early gene encoded short isoform (1a) lacks, a coiled-coil motif that mediates multimerization [19, 20]. Homers alter the function and distribution of metabotropic glutamate receptors (mGluRs) [20–22].
TRPC2 clones have poor surface expression in vitro and may require a chaperone for proper formation [23, 24]. Receptor transporting protein 1 (RTP1) and receptor expression enhancing protein 1 (REEP1) are putative transmembrane protein chaperones expressed in the main olfactory system, which target olfactory GPCRs to the membrane and form protein-protein interactions with olfactory GPCRs in vitro . RTP1 and REEP1 mRNAs are expressed in the mouse VNO, but as of yet, neither protein has been reported in VSNs, and have not been proposed to have any functional interactions with vomeronasal GPCRs .
Given the role of TRPC2 in chemosignal detection, the interactions of TRPC2 with adaptor proteins in other sensory systems, and chaperone mRNA expression in the VNO, we sought to test the hypothesis that TRPC2 forms protein-protein associations with partners that could alter channel function or localization. Specifically, we sought to identify interactions between TRPC2 and Homer family members and interactions between TRPC2 and RTP1 or REEP1. First, we demonstrate VNO protein expression of RTP1, Homer, and TRPC2. Next we describe novel, in vivo, interactions between TRPC2 and Homer 1b/c as well as TRPC2 and RTP1. Lastly, we demonstrate a physiological role for the interaction between RTP1 and TRPC2; in vitro co-expression of RTP1 with TRPC2 leads to increased cell-surface expression of functional TRPC2.
Homer, RTP1, and REEP1 are Expressed in the Vomeronasal Organ
Isoform-specific antisera were then utilized to further probe which Homer isoforms were predominantly found in the VNO. Homer 1b/c and Homer 3 were expressed in all neural tissues tested including the OB and the VNO (Figs. 1B and 1E). Repetition of SDS-PAGE and Western analysis using the same tissue extracts as in Fig. 1B resulted in no immunoreactive labelling with preimmune serum used to generate the homer 1b/c antiserum (Fig. 1C). Homer 2 was weakly detected in the VNO, but was found in the OB, as well as other brain regions tested (Fig. 1D). In our previous studies we have noted sexual dimorphism in the VNO [26, 27], therefore, male and female VNO lysates were separately probed with Homer antisera. An appreciable sex difference was not consistently detected (Figs. 1A-E). Equal protein loading was confirmed for all Western blots by stripping the blot and then re-probing for actin immunoreactivity (i.e. Additional File 1, Fig. S1).
RTP1 and REEP1 mRNAs have only been demonstrated in the mouse , therefore rat VNO cDNA was screened using gene-specific primers and reverse-transcriptase PCR (RT-PCR) for chaperone expression. RTP1 and REEP1 expression in the rat VNO is reported in Fig. 1F. Each RT-PCR reaction produced a single band and the identity of the band was confirmed by sequencing. An additional screening of RTP1 in the mouse main olfactory epithelium was also performed across various postnatal stages as reported in Additional File 1, Fig. S1.
Homer and RTP1 form protein-protein interaction with TRPC2
TRPC2 transfection efficiency in a heterologous expression system
RTP1 alters the subcellular localization of TRPC2 in vitro
Cell-surface TRPC2 is functionally detected
These experiments demonstrate that the putative olfactory receptor chaperone RTP1 interacts with not only proteins of the GPCR superfamily, but also with ion channels. This novel finding was demonstrated in vivo by a protein-protein interaction between TRPC2 and RTP1, and in vitro by a RTP1-dependent increase in TRPC2 surface expression. An ion channel complex consisting of TRPC2, Homer, and IP3R3 may exist in vivo. Our biochemical experiments indicate that TRPC2, the scaffold protein Homer 1b/c, and the ion channel IP3R3 form protein-protein interactions in the native VNO. To the best of our knowledge, an interaction between Homer and TRPC2 has not been demonstrated in any sensory system. Lastly, our data represent the first characterization of Homer expression in the rat VNO.
Our data demonstrating transcription of RTP1 and REEP1 in the rat VNO support the previous finding of these transcripts in the mouse VNO . The fact that our immunocytochemical characterization supports the detection of RTP1 protein in olfactory sensory neuron cilia, VSN microvilli, goblet cells and in the soft-palate while RTP1 is not detected in the non-sensory respiratory epithelium, may provide important clues as to function. In vitro experiments support RTP1 interactions with GPCRs associated with either odorant  or taste receptor families . Similar protein-protein interactions have not been found, however, with VRs .
Alternatively, MHC class 1b proteins have been found to associate with the V2Rs and thus may not function with TRPC2 [35, 36]. Allowing for several different protein associations including M10-VR, RTP1-TRPC2, and Homer-TRPC2-IP3R3 provides the pheromone transduction cascade with multiple regulatory sites (Fig. 8).
The immunoprecipitation data indicate that Homer is not expressed in the non-sensory areas of the VNO, rather it is expressed in VSN microvilli. Other data have indicated that TRPC channels can interact with Homer proteins . For example, TRP (the drosophila homologue of TRPC ) is involved in such interactions in the invertebrate photoreceptor . Functionally, Homer expression in the VSN would allow for receptor and channel modulation. Linking TRPC2 and IP3R3 via Homer 1 would ensure high-fidelity transmission of the calcium signal that flows through the open TRPC2 channel during chemosignal detection. Homer 3 does not form protein-protein interactions with TRPC2 . The inducible form of Homer (1a isoform) was not detected in the rat VNO. This short form lacks the coiled-coiled domain and would oppositely be predicted to disassemble a TRPC2-Homer 1-IP3R3 complex (see Fig. 8). Disruption of this interaction could alter TRPC2 activity in a manner similar to Homer 1a modulation of mGluR activity . In previous VSN recordings, disruption of the interaction between TRPC2 and IP3R3 resulted in a diminished chemosignal response . On the other hand, TRPC1 mutants lacking Homer binding sites formed spontaneously active channels when expressed in vitro  and gene-targeted deletion of Homer 1 increased TRPC1 activity in vivo [16, 38]. Thus, Homer 1 could provide the VSN signalling apparatus with flexibility in responding to and adapting to chemosignals. The interaction between TRPC2 and RTP1 could provide further regulation of TRPC2 and the response of VSNs to chemosignals, by modulation of total TRPC2 activity dependent upon surface expression driven by the chaperone. The recognition sequence for RTP1 binding will need to be investigated in future experiments.
The plasmid containing TRPC2-C14 had a low transfection efficiency in HEK293 cells. Transient transfection of a plasmid containing Kv1.3 produces an efficiency percentage of approximately 60% , whereas TRPC2 efficiency is only about 30%. Poor transfection efficiencies have been noted of some constructs using polycationic transfection reagents ; however, other researchers have tried alternative transient transfection methods with TRPC2 and reported comparably low efficiencies [23, 41]. It is interesting to speculate that the low transfection efficiency might be a result of apoptosis due to calcium cytotoxicity associated with spontaneously active TRPC2 channels. At least one other TRPC channel, TRPC4, has been demonstrated to be spontaneously active in HEK293 cells . In light of the facts that neither chaperone increased TRPC2 transfection efficiency nor did this efficiency match that of Kv1.3, a structurally similar channel , suggests that proper TRPC2 cellular distribution and function may require binding partners not present in our experiments. Lastly, RTP1 and REEP1 do not appear to be toxic to the HEK293 cells, as transfection efficiency did not decrease with their expression.
RTP1 and REEP1 appear to alter the sub-cellular distribution of TRPC2 in vitro. In HEK293 cells, TRPC2 immunolabeling is predominately in large deposits that are presumably vesicles, as imaged with laser confocal microscopy. Although every transfection condition with TRPC2 led to vesicular immunolabeling, the presence of either RTP1 or REEP1 seemed to shift expression of the channel toward the surface membrane. These data indicate a functional relationship for the RTP1-TRPC2 co-immunoprecipitation found in VNO tissue. Our data cannot distinguish the mechanism of TRPC2 surface expression, which could be the result of either increased TRPC2 inserted or inhibition of TRPC2 internalization.
Demonstration of robust Kv1.3 surface expression lends credence to the assumptions made earlier when comparing sub-cellular localization of TRPC2 in different transfection conditions. Namely, when transfected with chaperone, the TRPC2 immunolabeling signal was similar to the Kv1.3 immunolabeling in terms of subcellular distribution. A similar shift in the immunolabeling signal of olfactory receptors occurs when these receptors are expressed in vitro with RTP or REEP  and with unrelated GPCRs . It then follows that with the addition of chaperone, more TRPC2 is in the surface membrane. In support of this notion, in each transfection condition where TRPC2 and RTP1 were transfected together, TRPC2 was detected in the surface membrane. When expressed alone, TRPC2 was infrequently detected in the surface membrane. Increased TRPC2 surface expression was detected with both cell-surface biotinylation and with whole-cell electrophysiology.
The endogenous metabotropic ATP receptor pathway utilized in our experiments to investigate in vitro TRPC2 current is similar to the VNO sensory transduction pathway as each activates PLC (Fig. 8) [24, 45, 46]. The electrophysiological data indicate that the surface expressed TRPC2 is functional and able to respond to a signal transduction pathway similar to that present in the VNO. That heavily-beaded, and presumably highly expressing, TRPC2+ RTP1+ cells were minimally responsive to purinergic stimulation is not unexpected. TRPC3, another TRPC channel, gains and loses agonist-induced activity based on expression level . Alternatively, the TRPC2 protein level may have been high enough and the purinergic receptor protein level low enough, that any interaction between the two might be negligible due to stoichiometric limitations.
Regardless of the mechanism, these data suggest that the half-life residence of TRPC2 in the membrane is increased in the presence of RTP1. These results may indicate that the functional interaction between RTP1 and TRPC2 is one of membrane stabilization rather than trafficking. This is a different functional role for RTP1 from previously described  and is speculative. With both olfactory and gustatory GPCRs, RTP1 is presumed to traffic its target to the surface membrane [25, 34].
Based upon our current results in the context of current knowledge [1, 48, 49], we propose the following model (Fig. 8). A chemosignal binds to either VR-type GPCR, activation of which ultimately results in a TRPC2-dependent calcium influx across the surface membrane. Calcium can also enter the cytosol from the endoplasmic reticulum via IP3R3. Homer 1 binding at either TRPC2 terminus  (residues 303PPTLL and 953LPVPF) may alter the channel function. TRPC2 and mGluRs are both integral transmembrane proteins and, therefore, the known interactions of mGluR/IP3R  could be replaced by TRPC2/IP3R complex formation in the VNO. For example, Homer 1 may cluster IP3R3 to TRPC2 using Homer binding motifs (PPXF, PPXXF and LPSSP) on both channels in a similar fashion that Homers cluster IP3R3 to mGluR . This would be advantageous for at least two reasons. First, the products of phospholipase C hydrolysis of PIP2, IP3 and DAG, gate both IP3R3 and TRPC2. Maintaining close proximity of the second-messenger targets would increase the speed of the signalling cascade and decrease the amount of second-messenger lost due to errant diffusion. Second, as IP3R3 may adopt a conformation that favors opening upon calcium binding , tethering IP3R3 next to the calcium source would increase the speed of the signalling cascade. By tethering IP3R3 underneath TRPC2, Homer would be expected to increase the open probability of IP3R3 using calcium influx contributed through the surface channel TRPC2. Thus, Homer could both increase the speed and the efficiency of the TRPC2 signalling cascade as it does for mGluR cascades. Signal adaptation is likely to come from calcium-calmodulin inhibition of both IP3R3  and TRPC2 , calcium-activated big conductance potassium channels (BK) [55, 56] and metabolism of DAG into the lipid arachidonic acid [56, 57].
Although complexes of channels and adaptors have been found in the visual system, Homer is a relatively newly discovered protein, and appears to be involved in scaffolding, targeting, and localization. Our previous finding of a direct protein-protein interaction between IP3R3 and TRPC2 in the VNO neither ruled out an additional role for scaffolding proteins, such as the Homer family, nor a role for chaperones, such as REEP1 and RTP1. In fact, the addition of chaperones and the formation of an adaptor complex may be critical to channel function and eagerly warrants future experimentation.
The VNO expresses members of the Homer protein family. TRPC2 complexes with both IP3R3 and Homer1b/c in vivo in the VNO. RTP1 is expressed in the VNO, co-localizes with other members of the VNO transduction pathway and may be a member of the transduction pathway as it forms a protein-protein interaction with TRPC2 in vivo. In vitro, RTP1 appears to function as a chaperone of TRPC2, increasing the amount of functional channel in the surface membrane.
Animal care and maintenance
Postnatal Day 30 (P30) Sprague-Dawley rats were used for biochemistry experiments and were housed on a 12 h:12 h light:dark cycle in the Florida State University (FSU) vivarium. All procedures were performed in accordance with the FSU Animal Care and Use Committee and NIH-approved guidelines.
Solutions used for protein sample or tissue preparation, including phosphate buffered saline (PBS), lysis buffer (LB), wash buffer (WB), and protease and phosphatase inhibitor solution (PPI), were made as described in . Tissue extract buffer (TEB) was prepared as in . Cell-surface biotinylation solutions, including biotinylation lysis buffer and biotinylation quench buffer, were made as described by . Immunoblot stripping buffers, including tris stripping buffer (TSB) and sodium citrate stripping buffer (SCSB), were also made as described in Colley et. al. (2007). Electrophysiology solutions were prepared as in  and were as follows (in mM): intracellular pipette 150 KCl, 10 HEPES pH 7.2, 2 MgCl2, 10 glucose; extracellular bath 140 NaCl2, 10 HEPES pH 7.4, 4 KCl, 4 CaCl2, 1 MgCl2, and 10 glucose. All chemicals were obtained from either Sigma Chemical Company (St. Louis, MO, USA) or Fisher Scientific (Suwanne, GA, USA).
Plasmids and antibodies
All encoded cDNAs were downstream from a cytomegalovirus (CMV) promoter. TRPC2 clone 14, with an N-terminal myc-epitope (EQKLISEEDL), was prepared in the pcDNA3 vector and was a kind gift from Dr. L Birnbaumer (National Institute of Environmental Health Sciences) . RTP1 and REEP1 were in the pCI vector and were kind gifts of Dr. H. Matsunami (Duke University) . pCDM8 was a kind gift from Dr. Brian Seed (Harvard University) . DNA encoding human CD8 was amplified from pCDM8 and subcloned into the pcDNA3 vector (Carlsbad, CA, Invitrogen) between the BamH1 and EcoR1 restriction sites. cDNA encoding Kv1.3 was subcloned into the pcDNA3 vector (Carlsbad, CA, Invitrogen) at the unique HindIII restriction site within the multiple cloning region . Kv1.3 was also epitope-tagged via insertion of the myc sequence on the extracellular face of the channel between the S1 and S2 transmembrane domains .
T1NH, T2NH, and T3NH are anti-peptide polyclonal antibodies specific for the type-1, -2 and -3 IP3R isoforms, respectively, and were raised against the following amino terminal sequences (amino acid position in parentheses): T1NH = CLATGHYLAAEVDPDQEVDPDQ-DASR (308-326), T2NH = CPDYRDAQNEGKTVRDGKTVRDGELP (320-338) and T3NH = CENPSYKGDVSDPGDVSDPKAAGPGA (319-337). These antibodies were a generous gift of Dr Gregory Mignery (Loyola University Chicago, Stritch College of Medicine, Maywood, IL, USA) . An antiserum detecting TRPC2 as raised in guinea pig and directed against the N-terminal cytosolic domain CSSDASGAGPGGPLRNVE was a generous gift of Dr. Richard Axel (Columbia University, New York, NY, USA) . Anti-peptide polyclonal antibodies detecting the different forms of Homer were made by immunizing rabbits with the synthetic C-terminal peptides of Homer 1b/c (IFELTELRDNLAKLLECS), 2a/b (GKIDDLHDFRRGLSKLGTDN), or 3 (RLFELSELRE-GLARLAEAA) conjugated to thyroglobulin with glutaraldehyde . A fourth, rabbit polyclonal antiserum recognizing all Homer 1 (1a, 1b, and 1c) isoforms was generated against the full-length GST-Homer 1a fusion protein . These antibodies were a generous gift of Dr. Paul Worley (Johns Hopkins University, Baltimore, MD, USA). A polyclonal antibody recognizing all Homer proteins (Pan-Homer; 1a, 1b, 1c, 2a, 2b, 3) was made by immunizing rats with recombinant Homer 1a (AB5875, Chemicon/Millipore, St. Louis, MO, USA). The mouse monoclonal antiserum for the c-myc epitope was from Roche (Indianapolis, IN, USA). Rabbit polyclonal antiserum specific to RTP1 was a kind gift of Dr. Hiro Matsunami (Duke University, Durham, NC, USA) . When necessary to validate equal protein loading, a rabbit polyclonal antibody detecting cellular actin was used. The immunogen for this antibody was SGPSIVHRKCF attached to a Multiple Antigen Peptide (MAP) backbone (Sigma Chemical).
Tissue homogenization and Western blotting
Tissue extracts were prepared as in . Briefly, P30 rats were killed by CO2 inhalation, decapitated, and VNOs were rapidly dissected and placed in ice cold TEB with PPI. Samples on ice were sonicated (Sonic Dismembrator, Model 60, Fisher Scientific) twice for eight seconds on setting number five. Non-soluble matter was removed by ultracentrifugation (Beckman Coulter, Fullerton, CA, USA) at 37,000 × g for 30 min at 4°C. The supernatant was aliquoted and stored at -80°C until use. The resultant pellet was solubilized in 2% sodium dodecyl sulfate (SDS), aliquoted, and stored at -80°C in case the initial screening of the lysates did not yield membrane-associated fractions.
Immunoprecipitations were performed as in . In brief, P30 rat VNOs were homogenized with a size 20 Kontes glass tissue-homogenizer (Kontes Glass, Vineland, NJ, USA) on ice in LB with PPI. Lysis was continued on a Roto-Torque (Model 7637, Cole-Parmer Instruments, Vernon Hills, IL, USA) for 30 min at 4°C. Lysates were clarified by centrifugation at 15,000 × g (Eppendorf, Model 5415C, Westbury, NY, USA) for 10 min at 4°C and then precleared for 1 hour (hr) with 3 mg/ml protein A sepharose (GE Healthcare, Uppsalla). This was followed by another centrifugation step to remove the protein A sepharose. Proteins of interest (Homer 1b/c, Homer 2, Homer 3, TRPC2, IP3R3, RTP1) were immunoprecipitated from the clarified lysates by overnight incubation on a Roto-Torque at 4°C with 5 μg/ml of appropriate antiserum. Samples were then incubated for 3 hr with protein A sepharose and centrifuged as above. Immunoprecipitates were washed four times with 5 volumes of WB. Lysates and washed immunoprecipitates were diluted in SDS gel loading buffer containing  1 mM Na3VO4 and stored at -20°C until use.
Purified VNO tissue extracts or immunoprecipitated proteins were separated on 6 to 15% acrylamide gels by SDS-PAGE and electro-transferred to nitrocellulose membranes. Equal protein loading (30 μg) was controlled by Bradford protein assay (BioRad, Hercules, CA, USA) and confirmed by 0.1% Fast Green staining and α-β-actin labeling. The nitrocellulose membrane was blocked with 5% non-fat milk for 60 min, incubated overnight at 4°C in primary antisera against TRPC2 (1:2000), IP3R3 (1:2000), RTP1 (1:1000), Homer 1b/c (1:1000), Homer 2a/b (1:2000), Homer 3 (1:2000), Homer 1 isoforms (Worley, 1:1000), all Homer isoforms (Chemicon, 1:1000), or RTP1 (1:1000). Membranes were then incubated with horseradish peroxidase-conjugated species-specific secondary antibody (donkey anti-rabbit, Amersham Biosciences or rabbit anti-guinea pig, Sigma) for 90 min at room temperature (rt). Labelled protein was detected with enhanced chemiluminescence (ECL; Amersham Biosciences, Piscataway, NJ, USA) using Classic Blue autoradiography film BX (MidSci, St. Louis, MO, USA). To ensure equal loading, nitrocellulose membranes were stripped by incubating blots in eight ten-minute washes of TSB, followed by eight ten-minute washes of SCSB, then re-probed using α-β-actin (1:1000). Autoradiographs were scanned using a Hewlett-Packard Photosmart Scanner (Model 106-816; Hewlett-Packard, San Diego, CA, USA) and quantified by line scanning densitometry using Quantiscan Software (Biosoft, Cambridge, UK).
RNA extraction and reverse-transcriptase PCR
P30 rats were killed by CO2 inhalation, decapitated, and VNOs were rapidly dissected from the surrounding tissues and placed on dry ice. RNA was extracted using the SV Total RNA Isolation System as per the manufacturer's protocol (Promega, Madison, WI, USA). RNA purity and concentration was determined by UV spectroscopy (NanoDrop-1000, Wilmington, DE, USA). cDNA was reverse-transcribed using the ImPromII kit as per manufacturer's protocol (Promega). Gene-specific primers have been previously reported  and were as follows: RTP1_forward, AAGCGTGACCACAGATGAGTG; RTP1_reverse, GAGCAGAAGTTCCAGCCTGAG; REEP1_forward, CAATGAATTCCCACCATGGTGTCATGGATCATCTCCAGGC; REEP1_reverse, GACTAGCGGCCGCCTAGGCGGTGCCTGAGCTGCTAG. PCR products were resolved using 1.0% agarose gel electrophoresis and visualized via UV excitation of the incorporated ethidium bromide.
Human embryonic kidney cell (HEK293) maintenance and transfection
HEK 293 cells were maintained in minimum essential medium (MEM), 2% penicillin/streptomycin, and 10% FBS (Gibco BRL). Before transfection, cells were grown to confluence (~7 days), dissociated with trypsin, and re-plated to low density onto Corning substrate-coated plasticware on poly-d-lysine-treated glass coverslips as previously described . HEK293 cells were transfected for 4-5 hrs at 37°C with plasmid cDNA and LipofectamineTM transfection reagent (Invitrogen, Carlsbad, CA, USA) in OptiMEM serum-reduced media (Gibco BRL). Either 1.5 μg of plasmid DNA and 7.5 μl Lipofectamine were applied to 30-50% confluent glass coverslips in 35 mm dishes or 3.0 μg of plasmid DNA and 15 μl Lipofectamine were applied to 80-95% confluent 60 mm dishes, for immunocytochemical and biochemical experiments, respectively. pcDNA3 vector was used to normalize total DNA concentration in co-transfection conditions, as previously .
Thirty-six hrs post-transfection, HEK293 cells were washed in PBS and fixed in ice-cold St. Marie's fixative (1.0% acetic acid in 95% ethanol). Cells were washed three times in PBS and incubated for 30 min at rt in PBST-block (0.1% Triton x-100 in PBS and 1% Bovine Serum Albumin). Cells were immunolabeled with primary antisera diluted in PBST-block for 90 min at rt or overnight at 4°C with α-c-myc. Cells were then washed three times in PBS. The secondary antisera were applied at rt for 1.5 hr in PBS using fluorescein isothiocyanate-conjugated goat anti-mouse antiserum (1:200). Following three washes in PBS, cells were counter-stained with a five-minute incubation in diamidino-phenyindole (DAPI) in PBS (1:5000). The cells were mounted on glass slides with Vectashield (Vector Laboratories, Burlingame, CA, USA) to prevent photobleaching.
Tissue sections (~16 μm) were prepared from P30 rats that had been fixed-perfused (4% paraformaldehyde) and sucrose cryoprotected as previously described . Sections were air-dried on the bench for 60 minutes, re-hydrated with PBS, and then incubated at 80°C in 10 mM sodium citrate for 30 minutes for antigen retrieval . The sections were cooled and non-specific binding was blocked by a 60-minute incubation in blocking solution (5% normal goat serum/2.5% BSA/0.3% triton in PBS). During the block, the primary antibodies were treated with L-glutathione to cap reactive SH-functional groups. On ice, an L-glutathione/Tris-EDTA solution was diluted in the blocking solution containing the primary antiserum to a final concentration of 30 mM L-glutathione Tris-EDTA/1.25% normal goat serum/0.625% BSA/0.075% triton . After the block, sections were rinsed with Tris-EDTA - 0.3% triton and incubated with antiserum overnight in a darkened, humidified chamber. Antiserum for RTP1 was used at a final dilution of 1:200 and antisera for TRPC2 was used at 1:400. Sections to be stained using the ABC method were treated according to the manufacturer's protocol (Vector, Burlingham, CA, USA) and the precipitate was visualized with the chromagen AEC (Sigma). Immunofluorescence was detected with either donkey anti-rabbit Texas Red (Amersham Biosciences) or rabbit anti-guinea pig FITC (Sigma).
Conventional light microscopy was performed on an Axiovert S-100 (Zeiss, Thornwood, NY, USA) equipped with epifluoresence, an AxioCam camera (#412-312, Zeiss), and Axiovision data capture software (version 3.1, Zeiss). Images were captured with a pixel resolution of 1300 × 1030. Laser confocal microscopy was performed on an Axioplan 2 microscope attached to an LSM510 two-confocal system (Zeiss). FITC was excited at 488 and DAPI at 700 nm with argon/2 and titanium/sapphire lasers, respectively. Images were captured at 1024 × 1024 pixels resolution in LSM file format, and then were converted to a 16-bit TIFF file format using LsMB software (Zeiss). The TIFF file was opened in NIH ImageJ http://rsb.info.nih.gov/ij/ and a uniform rectangle of 200 pixels × 20 pixels was applied across the center of the cell (Z-axis) to obtain the plot density profile of the pixels underneath, as previously described .
Transfection efficiency analysis
Three to nine fields of view for each transfection condition (TRPC2 + pcDNA3, TRPC2 + RTP1, TRPC2 + REEP1, and Kv1.3 + pcDNA3) were captured under a fluorescent and either a brightfield or a DAPI emission. Each field of view was approximately 90,000 μm2. Transfection efficiency was calculated for each field of view as the ratio of the number of fluorescent cells divided by the total number of cells. Cell counts were performed using NIH ImageJ software.
Cell-surface biotinylation was performed as described . Briefly, either 24- or 48-hr post-transfection, HEK293 cells were washed with ice-cold PBS and then incubated with 1.0 mg/ml biotin (Pierce, Rockford, Ill, USA) in PBS for 30 min at 4°C. Following a PBS rinse, cells were incubated for 30 min at 4°C in quench buffer, rinsed again, and then lysed for 30 min at 4°C in lysis buffer. Lysed cells were scraped, triturated, and centrifuged at 12,000 rpm for 10 min at 4°C. The supernatants were collected and aliquots were set aside for confluency controls. Protein concentration was calculated using a Bradford assay (BioRad). Equal amounts of protein were brought up to 1 ml with PBS (pH 8.0) and incubated overnight at 4°C with 40 μl streptavidin-conjugated agarose-beads (Pierce). The beads were pelleted with a centrifugation of 12,000 rpm for 10 min at 4°C, washed, and then stored at -20°C until further use.
Hoffman modulation contrast optics was used to visualize cells at 40× magnification (Axiovert 135, Carl Zeiss). Thirty-six hrs post-transfection, HEK293 cells were rinsed with bath solution and incubated with anti-hCD8 beads (Dyna-Beads, Invitrogen) to mark transfected cells (either hCD8+/TRPC2+/RTP1- or cCD8+/TRPC2+/RTP1+) . Co-expression with CD8 allows visualization of cells taking up the cDNA encoding the channel or receptor of interest by marking transfected cells with a red polypropylene-antibody-linked bead. We  and others  have demonstrated that single cells take up multiple constructs equivalently and that the density of the beads is proportional to the expression of channel of interest. Cells were rinsed two times before beginning a recording session to remove any unbound beads. Cells were rinsed two more times before beginning a recording session. Electrophysiological records were analyzed using software from Microcal Origin (Northampton, MA) and Quattro Pro (Borland International, Scotts Valley, CA).
Patch electrodes were fabricated from Jencons glass (Jencons Limited, Bridgeville, PA) with pipette resistances between 9 and 14 MΩ. Macroscopic currents were recorded in the whole-cell configuration using an Axopatch-200B amplifier (MDS Analytical Technologies/Axon Instruments, Sunnyvale, CA), filtered at 5 kHz, digitized at 5 kHz, and stored for later analysis. All voltage signals were generated and data were acquired with the use of an Axon Digidata 1200 board with pClamp v9.2 software (Axon Instruments). Cells were held routinely at a holding potential (Vh) of -60 mV, stepped to 80 mV (Vc), held for 40 milliseconds (ms), and then changed to a ramp protocol by falling to -80 mV over 100 ms (-1.6 mV/ms). The total pulse duration was 140 ms and the inter pulse interval was 60 seconds. Several sweeps were taken over the 3-6 minutes after establishing the whole-cell configuration to establish a stable baseline. ATP stimulation was achieved with bath application of 0.5 ml of 500 μM for a final bath concentration of ~166 mM ATP. Peak inward current amplitude was measured at -80 mV from a subsequent sweep.
Numerical data were statistically analyzed using Prizm software (version 4, GraphPad, San Diego, CA, USA). One-way Analysis of Variance (ANOVA) with either a Student-Newman-Keuls (snk) or a Dunnett's post-hoc test was performed with statistical significance determined at the 95% confidence interval.
The authors would like to thank Dr. Richard Axel for donation of TRPC2 antisera, Dr. Gregory Mignery for donation of IP3R3 antisera and antigenic peptide, Dr. Hiro Matsunami for donation of RTP1 antiserum, and Drs. Paul Worley and Joseph Yuan for donation of Homer antisera and valuable assistance concerning methodology of tissue extracts. We would like to thank Dr. James M. Fadool and Ms. Kimberly Riddle for imaging assistance and Mr. Andre Irsigler for cloning assistance through the Department of Biological Science. We would like to thank Mr. Robert Daly, Mr. Jeffery Godbey, and Mr. Michael Henderson for routine technical assistance and mouse colony maintenance. We would also like to thank Mr. Charles Badland for excellent graphic design assistance and Dr. Patricio Velez for reading a previous version of the manuscript. This work was supported by two training grant mechanisms at the National Institutes of Health (NIH), T32 DC00044 and F31 DC006153 specifically through the National Institutes of Deafness and Communication Disorders (NIDCD).
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