JAM-A is a novel surface marker for NG2-Glia in the adult mouse brain
© Stelzer et al; licensee BioMed Central Ltd. 2010
Received: 12 August 2009
Accepted: 26 February 2010
Published: 26 February 2010
Junctional adhesion molecule-A (JAM-A) is an adhesive protein expressed in various cell types. JAM-A localizes to the tight junctions between contacting endothelial and epithelial cells, where it contributes to cell-cell adhesion and to the control of paracellular permeability.
So far, the expression pattern of JAM-A has not been described in detail for the different cell types of the adult brain. Here we show that a subset of proliferating cells in the adult mouse brain express JAM-A. We further clarify that these cells belong to the lineage of NG2-glia cells. Although these mitotic NG2-glia cells express JAM-A, the protein never shows a polarized subcellular distribution. Also non-mitotic NG2-glia cells express JAM-A in a non-polarized pattern on their surface.
Our data show that JAM-A is a novel surface marker for NG2-glia cells of the adult brain.
Junctional adhesion molecule-A (JAM-A, also called F11R or JAM-1) belongs to the family of junctional adhesion molecules, immunoglobulin-superfamily (Ig-SF) proteins characterized by a V-type and a C2-type Ig-like domain . JAM-A is expressed mainly by epithelial, endothelial cells and certain leukocyte subsets. JAM-A undergoes homophilic binding to promote homotypic interactions between adjacent cells. In addition, it undergoes heterophilic interactions with the leukocyte integrin αLβ2 which probably serves to regulate leukocyte interactions with endothelial cells . The homophilic binding is rather weak as it does not support cell adhesion of transfected cells to immobilized JAM-A Fc fusion proteins . Through its cytoplasmic tail JAM-A interacts with various PDZ domain-containing scaffolding proteins, and its homophilic binding activity is proposed to regulate the specific subcellular localization of these proteins . Interestingly, JAM-A directly interacts with the cell polarity protein PAR-3 [4, 5], a scaffolding protein that is highly conserved through evolution and that regulates various aspects of cell polarity in different cell types including epithelial cells, neurons, neuroblasts and the C. elegans zygote . By regulating the specific subcellular localization of PAR-3 JAM-A has been proposed to regulate the formation of tight junctions and apico-basal polarity in vertebrate epithelial cells . Recently, it has been shown that JAM-A is a marker for long-term repopulating hematopoietic stem cells in adult mice .
The broad distribution of JAM-A and its function as a marker for adult hematopoietic stem cells prompted us to investigate JAM-A expression in the adult brain.
Neural stem cells have the characteristics of glia cells [9, 10]. In the adult mammalian brain these stem cells represent a certain subtype of astrocytes . However, beside astrocytes and oligodendrocytes the adult mammalian brain contains a third type of macroglia, the so called NG2-glia cells. These cells exist abundantly in the grey and white matter of the adult central nervous system (CNS) and are almost as numerous as astrocytes . At least a subset of the NG2-glia cells of the adult CNS can proliferate and can function as progenitor cells for oligodendrocytes [12–15].
Here we show that JAM-A is indeed expressed in a certain population of mitotic cells in the brain. Through stainings with cell type-specific markers we identify NG-2-glia cells, and not neural stem cells or neuronal precursor cells, as the JAM-A-positive cell population. Thus, we provide evidence that JAM-A is a novel surface marker for NG2-glia cells in the brain.
A subset of proliferating SVZ cells express JAM-A
In a first set of experiments we wanted to find out whether JAM-A is expressed in proliferating stem or progenitor cells of the adult mouse brain and whether it shows an asymmetric distribution during mitosis. The most proliferative zone of the adult mouse brain is the subventricular zone (SVZ), a region where neural stem and progenitor cells are present and where new neurons for the olfactory bulb are produced.
Therefore we conclude that JAM-A is expressed in a subset of proliferating cells of the adult SVZ but is not asymmetrically inherited by one of the two daughter cells after mitosis.
JAM-A is not expressed in neural stem or progenitor cells
Next, we wanted to find out whether JAM-A expression is specific for a certain proliferating stem or progenitor cell type of the adult SVZ. If JAM-A is expressed in progenitor cells of the neuronal linage it either has to be expressed in neural stem cells (Typ-B cells), transient amplifying cells (Typ-C cells) or neuroblasts (Typ-A cells) . We used antibodies against GFAP to identify neural stem cells. Because GFAP is also expressed in astrocytes, we used cellular morphology in addition to GFAP-positive staining to discriminate between neural stem cells and astrocytes. Astrocytes usually show a stellate morphology while adult neural stem cells are more spindle shaped. Therefore, only GFAP-positive cells with spindle-like morphology are classified as adult neural stem cells. Antibodies against Mash1 were used to identify transient amplifying cells, antibodies against TuJ1 and PSA-NCAM (not shown) to identify neuroblasts.
Another cell type of the adult brain that is able to proliferate are microglia. Therefore we co-stained JAM-A with the microglia marker F4/80 (Figure 2D). Because no co-staining between JAM-A and F4/80 was detectable, we conclude that JAM-A is also not expressed on microglia.
JAM-A positive cells are abundant in all areas of the adult brain
JAM-A is not expressed in Neurons, Oligodendrocytes or Astroglia
JAM-A is a specific surface marker for NG2-Glia cells
From these observations, we conclude that JAM-A is a novel specific surface marker for NG2-glia of the adult mouse brain.
In this study we have analyzed the expression profile of the cell surface molecule JAM-A in the adult brain. We find that JAM-A is expressed on a subset of proliferating cells in the SVZ. To our surprise, these cells turned out to belong to the lineage of NG2-glia cells but not to the lineages of neural stem or progenitor cells (negative for the marker GFAP, Mash1 and TuJ1) or microglia (negative for F4/80). Thus, our findings indicate a cell type-specific expression pattern of JAM-A in the adult brain.
During asymmetric cell divisions, certain proteins are unevenly distributed among the two daughter cells [9, 10, 17]. This asymmetric distribution depends on their polarized subcellular distribution in the dividing cell. The Junctional adhesion molecule-A (JAM-A) is well known to show such a polarized enrichment on the apical pole of various polarized cell types [1, 18–20]. This opened the possibility that JAM-A is asymmetrically distributed during cell divisions. However, we found that JAM-A is evenly distributed in proliferating cells (Figure 1), which makes it likely that JAM-A is also equally inherited by both daughter cells and therefore is not a cell fate determinant. The role of JAM-A during cell division is not known. It is interesting to note that JAM-A has recently been found to regulate cell proliferation in epithelial cells. Genetic ablation of JAM-A in mice increased the proliferation of cells in colonic mucosal epithelial cells . These findings further support a role of JAM-A in the regulation of cell proliferation although the molecular mechanisms underlying this function of JAM-A still need to be revealed. JAM-A is known to be expressed by various cell types including epithelial cells, endothelial cells, hepatocytes, various leukocyte subsets, platelets, Sertoli cells, spermatozoa and hematopoietic stem cells [1, 8, 18–20]. It is thus not surprising that various functions have been attributed to JAM-A. One major function of JAM-A is the regulation of inflammatory responses by mediating specific interactions between leukocytes and endothelial cells . Here, for the first time, we described the expression of JAM-A on cells from the adult central nervous systems.
In the adult, the discovery of functional receptors, interactions with neurons and ability to respond to different harmful stimulations have implied roles of NG2 cells in facilitating neuronal network function, which may be important in brain inflammation, neurodegeneration and neuroregeneration . Therefore, it seems possible that JAM-A is involved in homotypic NG2-glia cell interactions or heterotypic interactions between NG2-glia cells and other cell types in the CNS through as yet unidentified receptors on these cells. Whether JAM-A is indeed mediating such cell-cell interactions in the central nervous system, like it has been shown for platelets and endothelial cells [23, 24], is currently unknown. In addition, JAM-A could also influence the adhesion of the NG2-glia cells to the extracellular matrix and thereby regulate the migration of the NG2-glia cells within the CNS. A role of JAM-A in cell migration by regulating the levels and/or activity of integrins has been described in epithelial cells, endothelial cells and neutrophil cells [25–28]. A second major function of JAM-A is the regulation of tight junction formation and of apico-basal polarity development in vertebrate epithelial cells . This function of JAM-A resides in its ability to interact with the cell polarity protein PAR-3 and to govern the specific subcellular localization of PAR3 and the PAR3-associated aPKC - PAR6 complex during junction formation [7, 29]. Whether the PAR3-PAR6-aPKC complex is regulated by JAM-A in NG-2 glia cells remains to be shown.
In summary, here we describe for the first time the expression of JAM-A on cells from the adult nervous system. JAM-A is exclusively expressed by a specific subtype of macroglia, the so-called NG2-glia cells.
The following primary antibodies were used: anti-JAM-A (rabbit) , anti-P-H3 (mouse, New England Biolabs), anti-GFAP (mouse, Millipore), anti-Mash1 (mouse, BD Bioscience), anti-TuJ1 (mouse, Covance), anti- GST-π (mouse, BD Bioscience), anti-NG2 (mouse, Millipore), anti-PDGFRα (rat, Millipore) and F4/80 (rat, Abcam). As secondary antibodies Alexa-fluorophore conjugated antibodies (goat, Invitrogen) were used. For staining of DNA Hoechst 33258 (Invitrogen) was used.
Mice were kept under standard conditions according to governmental rules and regulations. All stainings were repeated on sections from at least three different mice. The age of mice was between 60 and 90 days.
Adult mouse brains were fixed via perfusion with 120 mM phosphate buffer, pH 7.4 (PBS) followed by perfusion with 4% paraformaldehyde in PBS. After dissection the brains were post-fixed over night with 4% paraformaldehyde in PBS. Sagittal sections (40 μm) were prepared with a Vibratome (Leica). Sections were block with blocking buffer (100 mM Tris buffer, 0.5% Triton X-100, 0.1% sodium azide, 0.1% sodium citrate and 5% normal goat serum). Blocking was followed by incubation with primary and secondary antibodies, both diluted in the blocking solution. Images were collected by confocal microscopy using CEN software (Zeiss); image analysis was performed with the CEN software, Adobe Photoshop and the IMAGE J software.
All experiments involving mice have been conducted according to German Animal Welfare Act and have been approved by the responsible authorities (Landesamt für Natur, Umwelt und Verbraucherschutz Nordrhein-Westfalen).
The authors thank Eva Bunk, Anna-Lena Hillje and Maik Worlitzer for comments on the manuscript and Dr. Frank Kirchhoff (Göttingen) for the GFAP-GFP mice.
K.E.'s lab is supported by grants from the German Research Foundation (DFG) and from the Medical Faculty of the University Münster.
J. C. S.'s lab is supported by the German Research Foundation (Emmy Noether Program and SFB629), the Kompetenznetzwerk Stammzellforschung NRW, and the EU FP7 network EuroSyStem.
- Ebnet K, Suzuki A, Ohno S, Vestweber D: Junctional adhesion molecules (JAMs): more molecules with dual functions?. J Cell Sci. 2004, 117: 19-29. 10.1242/jcs.00930.View ArticlePubMedGoogle Scholar
- Weber C, Fraemohs L, Dejana E: The role of junctional adhesion molecules in vascular inflammation. Nat Rev Immunol. 2007, 7: 467-477. 10.1038/nri2096.View ArticlePubMedGoogle Scholar
- Cunningham SA, Arrate MP, Rodriguez JM, Bjercke RJ, Vanderslice P, Morris AP, et al.: A novel protein with homology to the junctional adhesion molecule. Characterization of leukocyte interactions. J Biol Chem. 2000, 275: 34750-34756. 10.1074/jbc.M002718200.View ArticlePubMedGoogle Scholar
- Ebnet K, Suzuki A, Horikoshi Y, Hirose T, Meyer Zu, Brickwedde MK, Ohno S, et al.: The cell polarity protein ASIP/PAR-3 directly associates with junctional adhesion molecule (JAM). EMBO J. 2001, 20: 3738-3748. 10.1093/emboj/20.14.3738.PubMed CentralView ArticlePubMedGoogle Scholar
- Itoh M, Sasaki H, Furuse M, Ozaki H, Kita T, Tsukita S: Junctional adhesion molecule (JAM) binds to PAR-3: a possible mechanism for the recruitment of PAR-3 to tight junctions. J Cell Biol. 2001, 154: 491-497. 10.1083/jcb.200103047.PubMed CentralView ArticlePubMedGoogle Scholar
- Suzuki A, Ohno S: The PAR-aPKC system: lessons in polarity. J Cell Sci. 2006, 119: 979-987. 10.1242/jcs.02898.View ArticlePubMedGoogle Scholar
- Rehder D, Iden S, Nasdala I, Wegener J, Brickwedde MK, Vestweber D, et al.: Junctional adhesion molecule-a participates in the formation of apico-basal polarity through different domains. Exp Cell Res. 2006, 312: 3389-3403. 10.1016/j.yexcr.2006.07.004.View ArticlePubMedGoogle Scholar
- Sugano Y, Takeuchi M, Hirata A, Matsushita H, Kitamura T, Tanaka M, et al.: Junctional adhesion molecule-A, JAM-A, is a novel cell-surface marker for long-term repopulating hematopoietic stem cells. Blood. 2008, 111: 1167-1172. 10.1182/blood-2007-03-081554.View ArticlePubMedGoogle Scholar
- Kosodo Y, Roper K, Haubensak W, Marzesco AM, Corbeil D, Huttner WB: Asymmetric distribution of the apical plasma membrane during neurogenic divisions of mammalian neuroepithelial cells. EMBO J. 2004, 23: 2314-2324. 10.1038/sj.emboj.7600223.PubMed CentralView ArticlePubMedGoogle Scholar
- Schwamborn JC, Berezikov E, Knoblich JA: The TRIM-NHL protein TRIM32 activates microRNAs and prevents self-renewal in mouse neural progenitors. Cell. 2009, 136: 913-925. 10.1016/j.cell.2008.12.024.PubMed CentralView ArticlePubMedGoogle Scholar
- Zhao C, Deng W, Gage FH: Mechanisms and functional implications of adult neurogenesis. Cell. 2008, 132: 645-660. 10.1016/j.cell.2008.01.033.View ArticlePubMedGoogle Scholar
- Nishiyama A, Komitova M, Suzuki R, Zhu X: Polydendrocytes (NG2 cells): multifunctional cells with lineage plasticity. Nat Rev Neurosci. 2009, 10: 9-22. 10.1038/nrn2495.View ArticlePubMedGoogle Scholar
- Nishiyama A, Watanabe M, Yang Z, Bu J: Identity, distribution, and development of polydendrocytes: NG2-expressing glial cells. J Neurocytol. 2002, 31: 437-455. 10.1023/A:1025783412651.View ArticlePubMedGoogle Scholar
- Reynolds R, Dawson M, Papadopoulos D, Polito A, Di Bello IC, Pham-Dinh D: The response of NG2-expressing oligodendrocyte progenitors to demyelination in MOG-EAE and MS. J Neurocytol. 2002, 31: 523-536. 10.1023/A:1025747832215.View ArticlePubMedGoogle Scholar
- Horner PJ, Thallmair M, Gage FH: Defining the NG2-expressing cell of the adult CNS. J Neurocytol. 2002, 31: 469-480. 10.1023/A:1025739630398.View ArticlePubMedGoogle Scholar
- Matthias K, Kirchhoff F, Seifert G, Huttmann K, Matyash M, Kettenmann H, et al.: Segregated expression of AMPA-type glutamate receptors and glutamate transporters defines distinct astrocyte populations in the mouse hippocampus. J Neurosci. 2003, 23: 1750-8.PubMedGoogle Scholar
- Knoblich JA: Mechanisms of asymmetric stem cell division. Cell. 2008, 132: 583-597. 10.1016/j.cell.2008.02.007.View ArticlePubMedGoogle Scholar
- Gliki G, Ebnet K, Aurrand-Lions M, Imhof BA, Adams RH: Spermatid differentiation requires the assembly of a cell polarity complex downstream of junctional adhesion molecule-C. Nature. 2004, 431: 320-324. 10.1038/nature02877.View ArticlePubMedGoogle Scholar
- Konopka G, Tekiela J, Iverson M, Wells C, Duncan SA: Junctional adhesion molecule-A is critical for the formation of pseudocanaliculi and modulates E-cadherin expression in hepatic cells. J Biol Chem. 2007, 282: 28137-28148. 10.1074/jbc.M703592200.View ArticlePubMedGoogle Scholar
- Shao M, Ghosh A, Cooke VG, Naik UP, Martin-DeLeon PA: JAM-A is present in mammalian spermatozoa where it is essential for normal motility. Dev Biol. 2008, 313: 246-255. 10.1016/j.ydbio.2007.10.013.PubMed CentralView ArticlePubMedGoogle Scholar
- Laukoetter MG, Nava P, Lee WY, Severson EA, Capaldo CT, Babbin BA, et al.: JAM-A regulates permeability and inflammation in the intestine in vivo. J Exp Med. 2007, 204: 3067-3076. 10.1084/jem.20071416.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang A, He BP: Characteristics and functions of NG2 cells in normal brain and neuropathology. Neurol Res. 2009, 31: 144-150. 10.1179/174313209X393555.View ArticlePubMedGoogle Scholar
- Babinska A, Kedees MH, Athar H, Ahmed T, Batuman O, Ehrlich YH, et al.: F11-receptor (F11R/JAM) mediates platelet adhesion to endothelial cells: role in inflammatory thrombosis. Thromb Haemost. 2002, 88: 843-850.PubMedGoogle Scholar
- Babinska A, Kedees MH, Athar H, Sobocki T, Sobocka MB, Ahmed T, et al.: Two regions of the human platelet F11-receptor (F11R) are critical for platelet aggregation, potentiation and adhesion. Thromb Haemost. 2002, 87: 712-721.PubMedGoogle Scholar
- Cera MR, Fabbri M, Molendini C, Corada M, Orsenigo F, Rehberg M, et al.: JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling. J Cell Sci. 2009, 122: 268-277. 10.1242/jcs.037127.View ArticlePubMedGoogle Scholar
- Mandell KJ, Babbin BA, Nusrat A, Parkos CA: Junctional adhesion molecule 1 regulates epithelial cell morphology through effects on beta1 integrins and Rap1 activity. J Biol Chem. 2005, 280: 11665-11674. 10.1074/jbc.M412650200.View ArticlePubMedGoogle Scholar
- Naik MU, Naik UP: Junctional adhesion molecule-A-induced endothelial cell migration on vitronectin is integrin alpha v beta 3 specific. J Cell Sci. 2006, 119: 490-499. 10.1242/jcs.02771.View ArticlePubMedGoogle Scholar
- Severson EA, Lee WY, Capaldo CT, Nusrat A, Parkos CA: Junctional adhesion molecule A interacts with Afadin and PDZ-GEF2 to activate Rap1A, regulate beta1 integrin levels, and enhance cell migration. Mol Biol Cell. 2009, 20: 1916-1925. 10.1091/mbc.E08-10-1014.PubMed CentralView ArticlePubMedGoogle Scholar
- Ludford-Menting MJ, Oliaro J, Sacirbegovic F, Cheah ET, Pedersen N, Thomas SJ, et al.: A network of PDZ-containing proteins regulates T cell polarity and morphology during migration and immunological synapse formation. Immunity. 2005, 22: 737-748. 10.1016/j.immuni.2005.04.009.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.