Altering the trajectory of early postnatal cortical development can lead to structural and behavioural features of autism
© Chomiak et al; licensee BioMed Central Ltd. 2010
Received: 14 January 2010
Accepted: 19 August 2010
Published: 19 August 2010
Autism is a behaviourally defined neurodevelopmental disorder with unknown etiology. Recent studies in autistic children consistently point to neuropathological and functional abnormalities in the temporal association cortex (TeA) and its associated structures. It has been proposed that the trajectory of postnatal development in these regions may undergo accelerated maturational alterations that predominantly affect sensory recognition and social interaction. Indeed, the temporal association regions that are important for sensory recognition and social interaction are one of the last regions to mature suggesting a potential vulnerability to early maturation. However, direct evaluation of the emerging hypothesis that an altered time course of early postnatal development can lead to an ASD phenotype remains lacking.
We used electrophysiological, histological, and behavioural techniques to investigate if the known neuronal maturational promoter valproate, similar to that in culture systems, can influence the normal developmental trajectory of TeA in vivo. Brain sections obtained from postnatal rat pups treated with VPA in vivo revealed that almost 40% of cortical cells in TeA prematurely exhibited adult-like intrinsic electrophysiological properties and that this was often associated with gross cortical hypertrophy and a reduced predisposition for social play behaviour.
The co-manifestation of these functional, structural and behavioural features suggests that alteration of the developmental time course in certain high-order cortical networks may play an important role in the neurophysiological basis of autism.
Autism spectrum disorder (ASD) is a behaviourally defined brain disorder affecting approximately 1 in 150 children . Autistic children exhibit impoverished verbal and non-verbal communication skills and reduced social interactions where they often bias their attention towards certain objects rather than the surrounding social situation . Children with ASD also display behavioural impairments in attention engagement and disengagement, do poorly in emotional discrimination and facial recognition, and fail to response to their own names [2–6]. It has been suggested that behavioural phenotypes of ASD are associated with maturational changes in cortical thickness and organization, particularly affecting pyramidal neurons [1, 7]. In addition, structural and functional abnormalities are particularly prominent in the temporal neocortex [1, 8–10], and associated target structures including the amygdala , that mediate auditory and visual object recognition and attention orientation [1, 11–14].
The underlying cellular and neurobiological mechanism(s) associated with ASD have remained elusive. Based on the work in autistic children, Susan Bryson has proposed that the expression of autistic behaviours may involve a hypersensitivity to sensory stimulation . Indeed, recent work using one rodent model of autism has provided some evidence to support this conjecture. For example, Markram's lab has shown that rats prenatally exposed to VPA frequently exhibit hyper-connectivity and enhanced plasticity in prefrontal neocortical networks [15, 16]. Hence, from a cellular level, increased neural activity in cortical networks may lead to abnormally noisy networks thus making it difficult for neural processing of certain sensory stimuli in the autistic brain .
The postnatal maturation trajectory of the neocortex is highly heterogeneous, exhibiting large regional variability in both structure and functional development [18–22]. This issue is, however, rarely addressed in the literature despite the fact that there is a growing realization that some of the key brain abnormalities of autism can be highly protracted and continue to evolve during postnatal life [23, 24]. This is not surprising given the fact that certain high-order brain regions important for social functions endure continued plastic changes and delayed postnatal maturation [25, 26]. For example, unlike some regions of the primary sensory and motor cortices, the speed of cortical maturation in high-order temporal association networks is significantly slower, often extending into adolescence [18–22]. This developmental feature suggests that the trajectory of temporal lobe development may be particularly sensitive to pathogenic factors that can influence the speed of neuronal maturation, especially during postnatal life . For example, culture work has shown that valproate (VPA), and analogous compounds, are potent epigenetic factors that can facilitate neuronal maturation in neurons [28–30]. However, whether VPA can influence the speed of postnatal maturation in vivo and whether this can be associated with structural and behavioural characteristics related to autism remains unknown.
Here we address the emerging hypothesis that it may be the time course of postnatal cortical development that is most disturbed in ASD. To this end, we examined the TeA network from animals treated with a VPA dosage previously used in vivo. We found that in addition to premature electrophysiological development of individual TeA cells, treated animals can exhibit gross cortical hypertrophy and a reduced predisposition for social play behaviour.
Reduced social (play) interaction associated with VPA treatment
Enlarged temporal association cortex in VPA-treated animals
Accelerated development in the TeA network
VPA does not appear to lead to cell death or the abnormal electrophysiological development of individual neurons
VPA has previously been associated with ASD based on the fact that children born to pregnant women taking VPA for seizure management had an elevated risk of developing ASD [15, 43]. However, although some of the abnormalities observed in the autistic brain are of prenatal origin, postnatal factors are also considered to play an important role [23, 27]. In fact, very little research has attempted to address how chemical agents may influence certain postnatal developmental processes . In our model, the adverse effect of VPA may be derived from a persistent effect on the temporal lobe as it undergoes a protracted and delayed maturation. For example, VPA has been frequently used in infants to manage seizure . Nevertheless, the importance of our experimental approach is that it has allowed us to directly examine the emerging hypothesis that it is an altered time course of brain development that is most disturbed in autism .
The timing of brain insult also seems to differ in terms of the phenotypes of ASD. For example, later exposure in humans has largely been associated with the nonsyndromic and sometimes high-functioning forms of ASD . By contrast, early exposure to an insult (e.g. first trimester) represents the time when the syndromic (i.e., multiple congenital anomalies and mental retardation) form of ASD is thought to be initiated . In this regard, the main physical developmental delay we observed here is a short delay in eye opening (see Figure 1) as reported previously . However, in an exceptional case, we found one pup among all littermates that had a persistently low body weight and delayed fur growth during early postnatal development which was excluded from our analysis. Interestingly, a persistently low body weight was also noted in most (if not all) animals in a previous study using an early prenatal VPA treatment protocol . Hence, the temporal difference between VPA treatment may produce certain biases related to behavioural and developmental phenotypes  in which later insults may more closely reflect nonsyndromic high-functioning forms of autism.
Cortical structural and electrophysiological changes induced by VPA
The most consistent neuropathological finding with respect to the autistic brain is cortical enlargement, particularly in the temporal lobe region [8, 9]. This is not surprising since the temporal lobe association networks are involved in many processes including attention, social interaction, object recognition, and biased competition where the object's neural representation dominates across multiple networks [1, 11–13, 35, 46, 47]. Post-mortem examination of some autistic brains has revealed an increase in cortical grey matter and hyper-convolution of some regions of the temporal lobe . In particular, Piven and colleagues noted that the largest relative increase in cortical grey matter was in the temporal lobe . Their value of around a 5% increase in the temporal lobe for typically developing controls is consistent with our results (Figure 2) and that of a recent study by Schumann and coworkers of around 10% .
The mechanism through which VPA influences neuronal development and cortical thickness is still not entirely clear and is likely complex and multifactorial. Although VPA is classically considered an anti-epileptic drug, a more recent study has shown that VPA appears to have a strong influence in mediating epigenetic modulation rather than enhanced GABA activity . Indeed, in our study the accelerated appearance of adult-like membrane properties in VPA-treated animals was obtained at a dosage that has been shown to inhibit histone deacetylases in vivo and activate epigenetic signalling pathways in the rat . These data indicate a possible role of chromatin remodelling in the control and execution of developmental gene programs involved in different aspects of neuronal maturation [28–30, 48]. However, future study is required to determine whether potential changes to the intracellular somatodendritic lipid and/or protein organization which may be contributing to the immature electrophysiological phenotype can also be related to the emergence of mature intrinsic properties [49, 50]. Nevertheless, in vivo VPA-treatment does appear to facilitate premature development of the TeA network. Interestingly however, inactivation of PTEN signalling mechanisms can also lead to increased cortical thickness , while mutations leading to the formation of tuberous sclerosis complex, which is strongly associated with ASD , can also result in more active cortical networks in both humans and rodents [52–54]. Thus, VPA may exert its effect on neuronal development and cortical anatomy through activation of gene programs related to activity development  and/or activity-dependent processes and signal transduction mechanisms [37, 55, 56].
VPA-induced behavioural changes
As noted by Leo Kanner in 1943, autistic children tend to avoid social interaction . Over the past several years, different indices of social interaction in animal models have been investigated and are consistently found to be reduced [17, 37, 57]. Social play behaviour has been well documented in the rat and is also considered one of the most widespread and least ambiguous forms of play amongst mammals . However, this type of social interaction, to our knowledge, has never previously been evaluated in relation to an autistic phenotype under these conditions.
It is well recognized that high-order temporal lobe networks play a critical role in auditory and visual processing, attention, and ultimately, social interaction [1, 11–14, 35, 36, 47, 58–60]. Autistic children often have difficulty with attention disengagement [5, 6] and frequently fail to react to their names so much so that parents often suspect deafness . Hence, the reduced social play behaviour we report here may reflect complex functional impairments in multiple sensorimotor domains. Indeed, a recent study has shown that the TeA network in rats can form a descending reticular-like network, providing top-down innervations to many cortical and subcortical structures including the amygdala . Thus, premature development of even a small population of TeA neurons may have a powerful influence on many downstream structures that are also involved in working memory, attention, and emotional behaviours.
The main finding of the present study indicates that early postnatal exposure to VPA, at a dosage known to inhibit histone deacetylases in vivo, can lead to synchronous developmental alterations reminiscent of ASD. Neurons from VPA-treated animals tended to acquired adult-like electrophysiological properties sooner during early postnatal development relative to controls. Furthermore, these electrophysiological changes were also associated with an increase in cortical thickness and a reduced predisposition for social interaction. Such a co-manifestation of features is consistent with the emerging theory that it is the time course of early postnatal development that may be most disturbed in ASD .
Preparation and electrophysiology
All experimental protocols were approved by the University of Calgary Conjoint Faculties Research Ethics Board. Under this protocol, animals are housed in The University of Calgary Animal Resource Center facility receiving constant care throughout the year. Briefly, newborn male and female Sprague-Dawley rats were socially caged after weaning at day P21. Frontal tissue sections were prepared on a Leica vibrotome (Germany). Slices (≈300 μm) were submerged in a recording chamber in oxygenated (95% 02; 5% CO2) artificial cerebrospinal fluid (aCSF). The aCSF had a final pH and osmolality of around 7.4 and 290-300 mOsm/kg respectively and contained (in mM): NaCl, 110; KCl, 3.5; MgCl2, 1.5; NaHCO3, 26; CaCl2, 2 and glucose, 10. The patch electrode solution contained (in mM): K-gluconate, 120; KCl, 10; Na-HEPES, 10; Na-GTP, 0.2; Na-ATP, 4. Whole-cell patch-clamp recordings were targeted to layer V of the posterior sector of the TeA-ectorhinal cortex (also known as TeV). The TeA region is identified, under DIC microscopy, as the region located dorsally to the rhinal fissure [62, 63]. The patch electrode solution had an osmolality and pH of around 285 mOsm and 7.2 respectively. The DC resistance of patch electrodes was 6-8 MΩ and recordings were made at 30-33°C. The bridge-balance and liquid-junction potential were corrected on-line. Intrinsic membrane properties were determined according to methods previously described .
in vivo valproate injections
The sodium salt of VPA (NaVPA; Sigma-Aldrich, St. Louis, MO) was dissolved in 0.9% saline (pH ≈7.3). Treated rats received a single i.p. injection on the order of 150 mg/kg/day . It should be noted that due to the species variation in valproate metabolism, direct dosage correlations can not be made with humans . However, from these data, we may predict that the functional dose in rat models of seizure would be slightly higher than that of humans, which is indeed the case [42, 44, 66]. Injections started on P6 and then continued for a maximum of 2 weeks. We also injected littermate control rats with 0.9% saline (pH ≈7.3). Recordings were made from juvenile (< 1 month) animals ranging from P12-P26.
Social (play) behaviour
Animals were isolated in macrolone cages measuring 43 cm × 15 cm × 28 cm for a period of 24 hours prior to testing similar to previously described . The test consisted of placing two rats (either saline injected or VPA injected) into the test cage for a period of 10 minutes. The animals (male/female pairs) were tested at the same age (i.e., age-matched littermates ranging from P35-P37) and weight (±20 g) as each other. Rough-and-tumble play behaviors (e.g., a direct "attack" to the nape on the neck of the other rat or "pinning" where one of the animals is lying with its dorsal surface on the floor of the test cage with the other animal standing over them), were measured as an indicator of social interaction .
Cued sensorimotor task
A total of 6 male and female animals (3 pairs; sex and age-match littermates) were used. Animals were water deprived for 47.5 h prior to behavioural testing but had unrestricted access to food. Following each session, animals had unrestricted access to water for 0.5 h. Training sessions consisted of ≥3 trials and began on postnatal day 23-30. Training occurred in the same test cage with an approximate 3-5 min break between trials. This also allowed for the paper towel bedding of the test cage to be replaced following each trial. The experiments were conducted in an isolated quiet room or sound-attenuating chamber and the location did not change once training had begun. The test cage consisted of a macrolone cage with a hole drilled a few centimeters from the bottom at one end (fixed reward location) just large enough to permit insertion of a water bottle spout. The wall was covered so the animal could not see the bottle contents that the spout was attached to. Latency measurements represent the time difference between auditory cue presentation and arrival at, and orientation to, the fixed reward location. A maximum cut-off of 20 seconds was used. A spout was always present but only when the cue was presented and the animal arrival at and orientated to the fixed reward location was the 30% sucrose solution available. A computer with a National Instruments A/D board (NI DAC-Card 6024E, 200 kSamples/s, 16 channels), a breakout box (National Instruments BNC-2020), and a high speed IEEE 1394a port was used to run in-house software to collect timing information. A simple on/off switch was connected to the computer to trigger synchronization between the tone output and latency measurements. The software generated a 10 kHz 80-85 dB sin wave output played through speakers placed at a short and fixed distance from the cage. The software recorded the button press, started the audio signal, and recorded the second button press when the rat touched the reward (i.e. water bottle spout). The time from the first button press to the second was logged in a data file and presented as a temporal latency.
Cortical thickness measurements and DAPI staining
Age-matched littermate brains from juvenile animals (e.g. P20-P23) were removed and fixed in 4% PFA overnight at 4°C. Frontal sections (150 μm) were then cut on a Leica vibrotome (Germany) in PBS. Images were acquired with a Zeiss microscope (Zeiss Axioplan Fluorescence Microscope; Carl Zeiss, Germany) and analyzed off-line. Cortical thickness measurements (i.e. distance between the underlying white matter to the pia) were made similar to that reported elsewhere . To reduce the potential for a masking effect of relatively small differences by the large variability between littermates, data was normalized to animals from the same litter. For 4',6-diamidino-2-phenylindole (DAPI) staining, sections were counterstained with DAPI (1:104) for 5 min. at room temperature followed by a 1 × PBS rinse 3 times for 5 min. Deep layer TeA cell counts corresponded to the region of electrophysiological recordings.
Signal acquisition and analysis was accomplished using Multi-clamp 700A and DIGIDATA 1322A 16-Bit data acquisition system and Clampex 9 programs allowing for data to be low-pass filtered at 2-8 kHz and digitized at ≥10 kHz (Axon Instruments, Inc. Forster City, CA, USA). Images analysis was accomplished using ImageJ software (NIH). Data are expressed as mean ± SEM. The statistical test and p value are noted when used. Two-tailed tests were used if not otherwise stated. Statistical analysis was accomplished with GraphPad software.
We would like to thank Sharmila Alams, Dr. Calvin Young, Dr. Keith Sharkey's Lab for providing technical assistance, and Dr. Wendy Edwards for helpful comments on an earlier version of this manuscript. This study was supported by grants from Canadian Institutes of Health Research, CIHR Regenerative Medicine Initiative and the Sinneave Family Foundation.
- Amaral DG, Schumann CM, Nordahl CW: Neuroanatomy of autism. Trends Neurosci. 2008, 31 (3): 137-145. 10.1016/j.tins.2007.12.005.View ArticlePubMed
- Kanner L: Autistic disturbances of affective contact. Nerv Child. 1943, 2: 217-250.
- Dalton KM, Nacewicz BM, Johnstone T, Schaefer HS, Gernsbacher MA, Goldsmith HH, Alexander AL, Davidson RJ: Gaze fixation and the neural circuitry of face processing in autism. Nat Neurosci. 2005, 8 (4): 519-526.PubMed CentralPubMed
- Bryson SE, Zwaigenbaum L, Brian J, Roberts W, Szatmari P, Rombough V, McDermott C: A prospective case series of high-risk infants who developed autism. J Autism Dev Disord. 2007, 37 (1): 12-24. 10.1007/s10803-006-0328-2.View ArticlePubMed
- Landry R, Bryson SE: Impaired disengagement of attention in young children with autism. J Child Psychol Psychiatry. 2004, 45 (6): 1115-1122. 10.1111/j.1469-7610.2004.00304.x.View ArticlePubMed
- Bryson SE: The Autistic Mind. The Neurobiology of Autism. Edited by: Bauman ML, Kemper TL. 2005, Baltimore, Maryland: The Johns Hopkins University Press, 34-44.
- van Kooten IA, Palmen SJ, von Cappeln P, Steinbusch HW, Korr H, Heinsen H, Hof PR, van Engeland H, Schmitz C: Neurons in the fusiform gyrus are fewer and smaller in autism. Brain. 2008, 131 (Pt 4): 987-999. 10.1093/brain/awn033.View ArticlePubMed
- Bailey A, Luthert P, Dean A, Harding B, Janota I, Montgomery M, Rutter M, Lantos P: A clinicopathological study of autism. Brain. 1998, 121 (Pt 5): 889-905. 10.1093/brain/121.5.889.View ArticlePubMed
- Hazlett HC, Poe M, Gerig G, Smith RG, Provenzale J, Ross A, Gilmore J, Piven J: Magnetic resonance imaging and head circumference study of brain size in autism: birth through age 2 years. Arch Gen Psychiatry. 2005, 62 (12): 1366-1376. 10.1001/archpsyc.62.12.1366.View ArticlePubMed
- Schumann CM, Bloss CS, Barnes CC, Wideman GM, Carper RA, Akshoomoff N, Pierce K, Hagler D, Schork N, Lord C, et al.: Longitudinal magnetic resonance imaging study of cortical development through early childhood in autism. J Neurosci. 2010, 30 (12): 4419-4427. 10.1523/JNEUROSCI.5714-09.2010.PubMed CentralView ArticlePubMed
- Komura Y, Tamura R, Uwano T, Nishijo H, Kaga K, Ono T: Retrospective and prospective coding for predicted reward in the sensory thalamus. Nature. 2001, 412 (6846): 546-549. 10.1038/35087595.View ArticlePubMed
- Naya Y, Yoshida M, Miyashita Y: Backward spreading of memory-retrieval signal in the primate temporal cortex. Science. 2001, 291 (5504): 661-664. 10.1126/science.291.5504.661.View ArticlePubMed
- Raz A, Buhle J: Typologies of attentional networks. Nat Rev Neurosci. 2006, 7 (5): 367-379. 10.1038/nrn1903.View ArticlePubMed
- Moeller S, Freiwald WA, Tsao DY: Patches with links: a unified system for processing faces in the macaque temporal lobe. Science. 2008, 320 (5881): 1355-1359. 10.1126/science.1157436.View ArticlePubMed
- Markram H, Rinaldi T, Markram K: The intense world syndrome - an alternative hypothesis for autism. Front Neurosci. 2007, 1 (1): 77-96. 10.3389/neuro.01.1.1.006.2007.PubMed CentralView ArticlePubMed
- Rinaldi T, Perrodin C, Markram H: Hyper-connectivity and hyper-plasticity in the medial prefrontal cortex in the valproic Acid animal model of autism. Front Neural Circuits. 2008, 2: 4-10.3389/neuro.04.004.2008.PubMed CentralView ArticlePubMed
- Schneider T, Przewlocki R: Behavioral alterations in rats prenatally exposed to valproic acid: animal model of autism. Neuropsychopharmacology. 2005, 30 (1): 80-89. 10.1038/sj.npp.1300518.View ArticlePubMed
- Gogtay N, Giedd JN, Lusk L, Hayashi KM, Greenstein D, Vaituzis AC, Nugent TF, Herman DH, Clasen LS, Toga AW, et al.: Dynamic mapping of human cortical development during childhood through early adulthood. Proc Natl Acad Sci USA. 2004, 101 (21): 8174-8179. 10.1073/pnas.0402680101.PubMed CentralView ArticlePubMed
- Luna B, Garver KE, Urban TA, Lazar NA, Sweeney JA: Maturation of cognitive processes from late childhood to adulthood. Child Dev. 2004, 75 (5): 1357-1372. 10.1111/j.1467-8624.2004.00745.x.View ArticlePubMed
- Golarai G, Ghahremani DG, Whitfield-Gabrieli S, Reiss A, Eberhardt JL, Gabrieli JD, Grill-Spector K: Differential development of high-level visual cortex correlates with category-specific recognition memory. Nat Neurosci. 2007, 10 (4): 512-522.PubMed CentralPubMed
- Scherf KS, Behrmann M, Humphreys K, Luna B: Visual category-selectivity for faces, places and objects emerges along different developmental trajectories. Dev Sci. 2007, 10 (4): F15-30. 10.1111/j.1467-7687.2007.00595.x.View ArticlePubMed
- Shaw P, Eckstrand K, Sharp W, Blumenthal J, Lerch JP, Greenstein D, Clasen L, Evans A, Giedd J, Rapoport JL: Attention-deficit/hyperactivity disorder is characterized by a delay in cortical maturation. Proc Natl Acad Sci USA. 2007, 104 (49): 19649-19654. 10.1073/pnas.0707741104.PubMed CentralView ArticlePubMed
- Bauman ML, Kemper TL: Neuroanatomic observations of the brain in autism: a review and future directions. Int J Dev Neurosci. 2005, 23 (2-3): 183-187. 10.1016/j.ijdevneu.2004.09.006.View ArticlePubMed
- Lathe R: Autism, Brain, and Environment. 2006, Philadelphia, PA: Jessica Kingsley Publishers
- Knudsen EI: Sensitive periods in the development of the brain and behavior. J Cogn Neurosci. 2004, 16 (8): 1412-1425. 10.1162/0898929042304796.View ArticlePubMed
- Knudsen EI, Heckman JJ, Cameron JL, Shonkoff JP: Economic, neurobiological, and behavioral perspectives on building America's future workforce. Proc Natl Acad Sci USA. 2006, 103 (27): 10155-10162. 10.1073/pnas.0600888103.PubMed CentralView ArticlePubMed
- Rodier PM: Environmental causes of central nervous system maldevelopment. Pediatrics. 2004, 113 (4 Suppl): 1076-1083.PubMed
- Hsieh J, Nakashima K, Kuwabara T, Mejia E, Gage FH: Histone deacetylase inhibition-mediated neuronal differentiation of multipotent adult neural progenitor cells. Proc Natl Acad Sci USA. 2004, 101 (47): 16659-16664. 10.1073/pnas.0407643101.PubMed CentralView ArticlePubMed
- Balasubramaniyan V, Boddeke E, Bakels R, Kust B, Kooistra S, Veneman A, Copray S: Effects of histone deacetylation inhibition on neuronal differentiation of embryonic mouse neural stem cells. Neuroscience. 2006, 143 (4): 939-951. 10.1016/j.neuroscience.2006.08.082.View ArticlePubMed
- Akhtar MW, Raingo J, Nelson ED, Montgomery RL, Olson EN, Kavalali ET, Monteggia LM: Histone deacetylases 1 and 2 form a developmental switch that controls excitatory synapse maturation and function. J Neurosci. 2009, 29 (25): 8288-8297. 10.1523/JNEUROSCI.0097-09.2009.PubMed CentralView ArticlePubMed
- Jessberger S, Nakashima K, Clemenson GD, Mejia E, Mathews E, Ure K, Ogawa S, Sinton CM, Gage FH, Hsieh J: Epigenetic modulation of seizure-induced neurogenesis and cognitive decline. J Neurosci. 2007, 27 (22): 5967-5975. 10.1523/JNEUROSCI.0110-07.2007.View ArticlePubMed
- Pellis SM, Pellis VC: Play fighting of rats in comparative perspective: a schema for neurobehavioral analyses. Neurosci Biobehav Rev. 1998, 23 (1): 87-101. 10.1016/S0149-7634(97)00071-7.View ArticlePubMed
- Wagner GC, Reuhl KR, Cheh M, McRae P, Halladay AK: A new neurobehavioral model of autism in mice: pre- and postnatal exposure to sodium valproate. J Autism Dev Disord. 2006, 36 (6): 779-793. 10.1007/s10803-006-0117-y.View ArticlePubMed
- Rinaldi T, Kulangara K, Antoniello K, Markram H: Elevated NMDA receptor levels and enhanced postsynaptic long-term potentiation induced by prenatal exposure to valproic acid. Proc Natl Acad Sci USA. 2007, 104 (33): 13501-13506. 10.1073/pnas.0704391104.PubMed CentralView ArticlePubMed
- Kolb B, Buhrmann K, McDonald R, Sutherland RJ: Dissociation of the medial prefrontal, posterior parietal, and posterior temporal cortex for spatial navigation and recognition memory in the rat. Cereb Cortex. 1994, 4 (6): 664-680. 10.1093/cercor/4.6.664.View ArticlePubMed
- Chelazzi L, Miller EK, Duncan J, Desimone R: A neural basis for visual search in inferior temporal cortex. Nature. 1993, 363 (6427): 345-347. 10.1038/363345a0.View ArticlePubMed
- Kwon CH, Luikart BW, Powell CM, Zhou J, Matheny SA, Zhang W, Li Y, Baker SJ, Parada LF: Pten regulates neuronal arborization and social interaction in mice. Neuron. 2006, 50 (3): 377-388. 10.1016/j.neuron.2006.03.023.PubMed CentralView ArticlePubMed
- Sia Y, Bourne JA: The rat temporal association cortical area 2 (Te2) comprises two subdivisions that are visually responsive and develop independently. Neuroscience. 2008, 156 (1): 118-128. 10.1016/j.neuroscience.2008.07.002.View ArticlePubMed
- Chomiak T, Hu B: Latent Neurons in the Mature Mammalian Cortex: keeping the "old" cortex "young". Society for Neuroscience Program no 3710: 2007. 2007, San Diego, CA
- Carleton A, Petreanu LT, Lansford R, Alvarez-Buylla A, Lledo PM: Becoming a new neuron in the adult olfactory bulb. Nat Neurosci. 2003, 6 (5): 507-518.PubMed
- Picken Bahrey HL, Moody WJ: Early development of voltage-gated ion currents and firing properties in neurons of the mouse cerebral cortex. J Neurophysiol. 2003, 89 (4): 1761-1773. 10.1152/jn.00972.2002.View ArticlePubMed
- Bittigau P, Sifringer M, Genz K, Reith E, Pospischil D, Govindarajalu S, Dzietko M, Pesditschek S, Mai I, Dikranian K, et al.: Antiepileptic drugs and apoptotic neurodegeneration in the developing brain. Proc Natl Acad Sci USA. 2002, 99 (23): 15089-15094. 10.1073/pnas.222550499.PubMed CentralView ArticlePubMed
- Moore SJ, Turnpenny P, Quinn A, Glover S, Lloyd DJ, Montgomery T, Dean JC: A clinical study of 57 children with fetal anticonvulsant syndromes. J Med Genet. 2000, 37 (7): 489-497. 10.1136/jmg.37.7.489.PubMed CentralView ArticlePubMed
- Siemes H, Spohr HL, Michael T, Nau H: Therapy of infantile spasms with valproate: results of a prospective study. Epilepsia. 1988, 29 (5): 553-560. 10.1111/j.1528-1157.1988.tb03760.x.View ArticlePubMed
- Coleman M, Betancur C: Introduction. The Neurology of Autism. Edited by: Coleman M. 2005, New York, NY: Oxford University Press, Inc, 3-39. full_text.View Article
- Chomiak T, Peters S, Hu B: Functional architecture and spike timing properties of corticofugal projections from rat ventral temporal cortex. J Neurophysiol. 2008, 100 (1): 327-335. 10.1152/jn.90392.2008.View ArticlePubMed
- Mooney DM, Zhang L, Basile C, Senatorov VV, Ngsee J, Omar A, Hu B: Distinct forms of cholinergic modulation in parallel thalamic sensory pathways. Proc Natl Acad Sci USA. 2004, 101 (1): 320-324. 10.1073/pnas.0304445101.PubMed CentralView ArticlePubMed
- Hsieh J, Gage FH: Epigenetic control of neural stem cell fate. Curr Opin Genet Dev. 2004, 14 (5): 461-469. 10.1016/j.gde.2004.07.006.View ArticlePubMed
- Caron C, Boyault C, Khochbin S: Regulatory cross-talk between lysine acetylation and ubiquitination: role in the control of protein stability. Bioessays. 2005, 27 (4): 408-415. 10.1002/bies.20210.View ArticlePubMed
- Rosenbluth J: Subsurface cisterns and their relationship to the neuronal plasma membrane. J Cell Biol. 1962, 13: 405-421. 10.1083/jcb.13.3.405.PubMed CentralView ArticlePubMed
- Bolton PF, Park RJ, Higgins JN, Griffiths PD, Pickles A: Neuro-epileptic determinants of autism spectrum disorders in tuberous sclerosis complex. Brain. 2002, 125 (Pt 6): 1247-1255. 10.1093/brain/awf124.View ArticlePubMed
- Iida K, Otsubo H, Mohamed IS, Okuda C, Ochi A, Weiss SK, Chuang SH, Snead OC: Characterizing magnetoencephalographic spike sources in children with tuberous sclerosis complex. Epilepsia. 2005, 46 (9): 1510-1517. 10.1111/j.1528-1167.2005.14005.x.View ArticlePubMed
- Meikle L, Talos DM, Onda H, Pollizzi K, Rotenberg A, Sahin M, Jensen FE, Kwiatkowski DJ: A mouse model of tuberous sclerosis: neuronal loss of Tsc1 causes dysplastic and ectopic neurons, reduced myelination, seizure activity, and limited survival. J Neurosci. 2007, 27 (21): 5546-5558. 10.1523/JNEUROSCI.5540-06.2007.View ArticlePubMed
- Wang Y, Greenwood JS, Calcagnotto ME, Kirsch HE, Barbaro NM, Baraban SC: Neocortical hyperexcitability in a human case of tuberous sclerosis complex and mice lacking neuronal expression of TSC1. Ann Neurol. 2007, 61 (2): 139-152. 10.1002/ana.21058.View ArticlePubMed
- Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD: Astrocytes promote myelination in response to electrical impulses. Neuron. 2006, 49 (6): 823-832. 10.1016/j.neuron.2006.02.006.PubMed CentralView ArticlePubMed
- Chomiak T, Hu B: What is the optimal value of the g-ratio for myelinated fibers in the rat CNS? A theoretical approach. PLoS One. 2009, 4 (11): e7754-10.1371/journal.pone.0007754.PubMed CentralView ArticlePubMed
- Markram K, Rinaldi T, La Mendola D, Sandi C, Markram H: Abnormal fear conditioning and amygdala processing in an animal model of autism. Neuropsychopharmacology. 2008, 33 (4): 901-912. 10.1038/sj.npp.1301453.View ArticlePubMed
- Shi C, Davis M: Visual pathways involved in fear conditioning measured with fear-potentiated startle: behavioral and anatomic studies. J Neurosci. 2001, 21 (24): 9844-9855.PubMed
- Layton BS, Toga AW, Horenstein S, Davenport DG: Temporal pattern discrimination survives simultaneous bilateral ablation of suprasylvian cortex but not sequential bilateral ablation of insular-temporal cortex in the cat. Brain Res. 1979, 173 (2): 337-340. 10.1016/0006-8993(79)90633-4.View ArticlePubMed
- Campeau S, Davis M: Involvement of subcortical and cortical afferents to the lateral nucleus of the amygdala in fear conditioning measured with fear-potentiated startle in rats trained concurrently with auditory and visual conditioned stimuli. J Neurosci. 1995, 15 (3 Pt 2): 2312-2327.PubMed
- Zwaigenbaum L, Bryson S, Rogers T, Roberts W, Brian J, Szatmari P: Behavioral manifestations of autism in the first year of life. Int J Dev Neurosci. 2005, 23 (2-3): 143-152. 10.1016/j.ijdevneu.2004.05.001.View ArticlePubMed
- Paxinos G, Watson C: The Rat Brain: In stereotaxic coordinates. 1998, New York: Academic Press, 4
- Paxinos G: The rat nervous system. 2004, Amsterdam: Elsevier Academic Press, 3
- Cruikshank SJ, Lewis TJ, Connors BW: Synaptic basis for intense thalamocortical activation of feedforward inhibitory cells in neocortex. Nat Neurosci. 2007, 10 (4): 462-468.PubMed
- Loscher W: Serum protein binding and pharmacokinetics of valproate in man, dog, rat and mouse. J Pharmacol Exp Ther. 1978, 204 (2): 255-261.PubMed
- Jeavons PM: Non-dose-related side effects of valproate. Epilepsia. 1984, 25 (Suppl 1): S50-55. 10.1111/j.1528-1157.1984.tb05638.x.View ArticlePubMed