Volume 10 Supplement 1

Eighteenth Annual Computational Neuroscience Meeting: CNS*2009

Open Access

TRPC channels activated by group I mGluR in Entorhinal pyramidal neurons support integration of low frequency (<10 Hz) synaptic inputs

BMC Neuroscience200910(Suppl 1):P26

DOI: 10.1186/1471-2202-10-S1-P26

Published: 13 July 2009

Background

Studies on animal behavior have shown that working memory functions are impaired when group I metabotropic glutamate receptors (mGluR1/5) are blocked. Entorhinal cortex (EC) has a key role in producing working memory function and enabling long-term memory formation in the hippocampus. Importantly, frequencies observed in EC during behavior are low, typically at or below theta (4–8 Hz). We have in recent work [2] shown experimentally that in EC pyramidal cells following synaptic stimulation of extrasynaptically located mGluR1/5, canonical transient receptor potential channels (TRPC) can be activated. We hypothesize that the presence of mGluR activated TRPC channels constitutes a mnemonic component, working in parallel with the ionotropic synaptic receptors AMPA and NMDA, each of which with a distinct time window for integration. Thus, TRPC channels with their slow kinetics could be capable of effectively integrating the low frequencies observed in EC.

Methods

We are using a multicompartmental model including calcium dynamics and several ion channels. It is based on a model of a CA1 pyramidal cell implemented in NEURON [1] and extended with mechanisms for mGluR/TRPC activation. Synaptic glutamate spillover activates mGluRs, which in turn sensitizes the TRPC channel. This effectively results in a translation of the calcium gating curve. To study the summation of low frequency input, we have applied synaptic inputs to the apical dendrite and observed how the resulting TRPC depolarization relates to the faster EPSPs following activation of AMPA and NMDA receptors. We also studied the TRPC dynamics in terms of stimulation frequency and duration.

Results

In figure 1 the individual contribution to membrane depolarization from ionotropic (AMPA+NMDA) and metabotropic (mGluR/TRPC) receptor activation respectively is plotted for a range of frequencies at steady state. The average membrane potential is increasing linearly for ionotropic activation, since the corresponding EPSPs are too brief to effectively integrate at low frequencies. The TRPC current, however, is effectively integrating the synaptic inputs already at low (around 1–2 Hz) frequencies, due to its slow time dynamics. Figure 2 shows the TRPC activation dynamics. The largest increase in channel current occurs for frequencies around 3 Hz, and below durations of 3 s.
https://static-content.springer.com/image/art%3A10.1186%2F1471-2202-10-S1-P26/MediaObjects/12868_2009_Article_1211_Fig1_HTML.jpg

Figure 1

https://static-content.springer.com/image/art%3A10.1186%2F1471-2202-10-S1-P26/MediaObjects/12868_2009_Article_1211_Fig2_HTML.jpg

Figure 2

Discussion

We have shown that the mGluR activated TRPC currents in Entorhinal cortex might play an important role for integrating synaptic inputs at low frequencies and durations of a few seconds. Both of these ranges lie in a behaviorally relevant domain.

Authors’ Affiliations

(1)
Dept. of Computational biology, School of Computer Science and Communication; Stockholm Brain Institute, Royal Institute of Technology, AlbaNova University Center

References

  1. Poirazi P, Brannon T, Mel BW: Arithmetic of subthreshold synaptic summation in a model CA1 pyramidal cell. Neuron. 2003, 37: 977-987. 10.1016/S0896-6273(03)00148-X.PubMedView ArticleGoogle Scholar
  2. Yoshida M, Fransén E, Hasselmo ME: mGluR-dependent persistent firing in entorhinal cortex layer III neurons. Eur J Neurosci. 2008, 28: 1116-1126. 10.1111/j.1460-9568.2008.06409.x.PubMed CentralPubMedView ArticleGoogle Scholar

Copyright

© Petersson and Fransén; licensee BioMed Central Ltd. 2009

This article is published under license to BioMed Central Ltd.

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