Imaging cytoplasmic cAMP in mouse brainstem neurons
© Mironov et al; licensee BioMed Central Ltd. 2009
Received: 05 August 2008
Accepted: 27 March 2009
Published: 27 March 2009
cAMP is an ubiquitous second messenger mediating various neuronal functions, often as a consequence of increased intracellular Ca2+ levels. While imaging of calcium is commonly used in neuroscience applications, probing for cAMP levels has not yet been performed in living vertebrate neuronal tissue before.
Using a strictly neuron-restricted promoter we virally transduced neurons in the organotypic brainstem slices which contained pre-Bötzinger complex, constituting the rhythm-generating part of the respiratory network. Fluorescent cAMP sensor Epac1-camps was expressed both in neuronal cell bodies and neurites, allowing us to measure intracellular distribution of cAMP, its absolute levels and time-dependent changes in response to physiological stimuli. We recorded [cAMP]i changes in the micromolar range after modulation of adenylate cyclase, inhibition of phosphodiesterase and activation of G-protein-coupled metabotropic receptors. [cAMP]i levels increased after membrane depolarisation and release of Ca2+ from internal stores. The effects developed slowly and reached their maximum after transient [Ca2+]i elevations subsided. Ca2+-dependent [cAMP]i transients were suppressed after blockade of adenylate cyclase with 0.1 mM adenylate cyclase inhibitor 2'5'-dideoxyadenosine and potentiated after inhibiting phosphodiesterase with isobutylmethylxanthine and rolipram. During paired stimulations, the second depolarisation and Ca2+ release evoked bigger cAMP responses. These effects were abolished after inhibition of protein kinase A with H-89 pointing to the important role of phosphorylation of calcium channels in the potentiation of [cAMP]i transients.
We constructed and characterized a neuron-specific cAMP probe based on Epac1-camps. Using viral gene transfer we showed its efficient expression in organotypic brainstem preparations. Strong fluorescence, resistance to photobleaching and possibility of direct estimation of [cAMP] levels using dual wavelength measurements make the probe useful in studies of neurons and the mechanisms of their plasticity. Epac1-camps was applied to examine the crosstalk between Ca2+ and cAMP signalling and revealed a synergism of actions of these two second messengers.
cAMP is an ubiquitous second messenger which regulates a wide variety of cellular events and processes ranging from metabolism and gene expression, cell division and migration, exocytosis and secretion, to memory formation and cardiac contractility . On the one hand, cAMP-signalling cascades are associated with many vital functions in the CNS that can be exemplified by cAMP-related changes in neuronal activity , dendritic and axonal growth , spine function , synaptic activity and neuronal plasticity , and gene expression  etc. On the other hand, inadequate cAMP signalling may lead to long-term disturbances of neuronal functions . cAMP-dependent enzymes are well characterized in terms of their affinity, kinetics and mechanisms of their modulation . In order to understand how cAMP-dependent signalling mechanisms operate in living neurons, appropriate tools are needed to monitor spatial and time-dependent changes of cAMP levels.
Direct imaging of cAMP became possible only recently. Two classes of probes have been developed – one based on protein kinase A (PKA) and utilizing fluorescence resonance energy transfer (FRET) between fluorescein and rhodamine  or CFP and YFP , and the other exploiting the exchange protein directly activated by cAMP, namely Epac [10–12]. PKA-based cAMP probes have slow response times, making them not very suitable for monitoring the rapid changes in [cAMP]i which occur in living cells. Originally designed Epac has good sensitivity and temporal resolution [10, 11]. Both features are conserved in Epac-1-camps , its smaller size allowing it to be incorporated into viral vectors with limited genome sizes such as AAV. In comparison with the common methods of transfection in primary cultures  and organotypic slices , the efficacy of viral gene transfer in postmitotic neurons is much higher. We recently used adeno-associated viruses (AAV) driving transgene expression by the strictly neuron-specific synapsin 1 gene promoter  to selectively express GFP in neurons in organotypic brainstem slices  containing pre-Bötzinger complex (preBötC), the latter constituting the rhythm-generating part of the respiratory network [17–19]. An important step in further understanding spatial and functional organisation of the respiratory network now is the expression of fluorescent sensors in respiratory neurons and measuring their responses to physiological stimuli.
Respiratory activity is crucially dependent on cAMP signalling, which can be dynamically modulated in specific physiological and pathological conditions . In this study we aimed to specifically target Epac1-camps to brainstem neurons. We characterised the sensitivity and specificity of the sensor to report intraneuronal cAMP. We measured [cAMP] changes after modulation of adenylate cyclase (AC), phosphodiesterase (PDE) and activation of G-protein-coupled metabotropic receptors. [cAMP] changes were tightly related to calcium transients, which are important for respiratory rhythmogenesis . Both depolarisation-triggered Ca2+ entry and agonist-induced Ca2+ release from endoplasmic reticulum (ER) led to cAMP increase caused by enhancement of AC activity by calcium. Paired calcium increases produced a non-linear summation of subsequent [cAMP]i increases. This was abolished after PKA inhibition, thus indicating an important role for phosphorylation in the dynamic interplay between Ca2+ and cAMP, previously documented in non-excitable cells .
Mean [cAMP]i changes observed after modulation of cAMP signalling pathway and activation of G-protein coupled glutamate and serotonin receptors
1.62 ± 0.12
1.45 ± 0.07
-0.12 ± 0.05
1.19 ± 0.08
0.74 ± 0.07
0.81 ± 0.05
Mean peak [Ca2+]i and [cAMP]i increases due to membrane depolarisation and Ca2+ release applications in the presence of drugs modulating cAMP and Ca2+ homeostasis.
0.44 ± 0.07
0.72 ± 0.08
0.24 ± 0.06
0.31 ± 0.05
0.01 ± 0.01
1.52 ± 0.09
3.22 ± 0.17
0.25 ± 0.02
0.94 ± 0.08
0.04 ± 0.01
0.39 ± 0.07
0.52 ± 0.08
0.24 ± 0.06
0.01 ± 0.01
0.31 ± 0.05
1.44 ± 0.08
2.24 ± 0.07
0.36 ± 0.03
0.04 ± 0.01
1.42 ± 0.08
Removal of extracellular Ca2+ or introduction of 0.1 mM Cd2+, which blocks all pathways for Ca2+ entry into the cell, abolished depolarisation-induced [cAMP]i changes (Table 2). The blockade of Ca2+ entry did not modify the effects of ATP (Fig. 3D). This indicates that only calcium release after activation of metabotropic P2y receptors is important and ionotropic P2X receptors are not involved in ATP-induced [cAMP]i increases. The effects were occluded after depletion of ER with thapsigargin (Table 2). This treatment also reduced the effects caused by membrane depolarisations by about 1/3 (Fig. 3D, Table 2) indicating contribution of Ca2+-induced Ca2+ release (CICR ) to [cAMP]i responses.
It was recently found in presynaptic boutons of Drosophila neurons  that Ca2+-related Epac1-camps responses can be mediated by changes in [cGMP]i. This apparently contradicts the fact that the affinity of the sensor to cGMP (Kd = 11 μM) is by about one order of magnitude lower than that for cAMP . However, the presynaptic calcium transients may have considerably higher amplitude and cause AC inactivation (see Discussion) which would make [cGMP]i changes dominant. In order to examine whether cGMP changes can underlie, in part, the responses of Epac1-camps, we applied guanylyl cyclase inhibitor 1H-[1, 2, 4]-oxadiazolo- [4,3-a]-quinoxalin-1-one (ODQ, 100 μM), the NOS inhibitor N-monomethyl-L-arginine (L-NMMA), and the NO donor S-nitroso-N-acetylpenicillamine (SNAP, 300 μM). These drugs were applied at concentrations at which they showed distinct effects on the respiratory motor output in vivo , but they did not modify the signals of Epac1-camps (n = 3 for each treatment).
Many vitally important processes which determine the life and fate of neurons are controlled by cAMP and Ca2+, which often act in parallel . In non-excitable cells the fluctuations of [Ca2+]i and [cAMP]i are often interrelated  and linked via adenylate cyclase  or phosphodiesterase . AC has eight different isoforms which can be stimulated and inhibited by Ca2+. The stimulation is characteristic for AC1 and AC8 which are abundant in the brain and activated by calmodulin. All AC isoforms are inhibited by supramicromolar concentrations of Ca2+ (> 10 μM) binding to a low-affinity site in the catalytic AC domain . We recorded calcium transients of much smaller amplitude, therefore a Ca2+-mediated inhibition of AC should not play any significant role in the effects reported here. It may however become significant in domains which are located close to the exit of Ca2+ from the channels producing calcium gradients which can reach values as high as 100 μM  and locally limit the production of cAMP.
The levels of Ca2+ and cAMP are often changed by various neurotransmitters and [cAMP]i changes may be further differentially decoded downstream through, for example, PKA, Epac, cAMP-binding protein (CREB) and Rap to initiate events such as gene expression and cell differentiation. PKA-mediated phosphorylation of channels is important for normal functioning of the channels that mediate entry of Ca2+ into the cytoplasm and its release from internal stores. These phosphorylation/dephosphorylation reactions thus close a regulatory Ca2+/cAMP feedback.
In comparison to Ca2+, we currently know much less about the changes in intracellular cAMP which can occur in relation to neuronal activity. Increasing applications of cAMP sensors developed in the last decade [8–12] to different neuronal preparations will definitely bring new knowledge in this area. Although until now such studies have been made only in some cell lines  and isolated neurons in primary culture , they have already revealed the complexity of cAMP dynamics. The neurons in living tissue present the next level of sophistication, as their properties can be modified by interactions with neighbouring neurons and surrounding glial cells. Intrinsic electrical activity of neurons add another important factor, because ensuing calcium fluxes of different amplitude and time-courses and spontaneous calcium release from internal stores should substantially modulate spatial [cAMP]i patterns on different time scales. How they are involved in different vital cellular functions such as neuronal plasticity, differentiation and development remains to be established.
Currently available cAMP sensors are bulky proteins and the first obstacle in cAMP imaging concerns the delivery of the probe into the cytoplasm. A straightforward way is a single cell injection  with well known difficulties. Transgenic animals would be an ideal solution and this approach has been successfully used to study cAMP signalling in the heart , pancreatic islets  and the neurons of fruit-flies . Non-cytotoxic viral gene transfer presents an alternative not limited in its application by the animal or tissue [15, 38]. It can be applied to any cell population given that the sensor is targeted to the specific cell type. We used a strictly neuron-specific promoter  to deliver the cAMP sensor  into neurons in slices. As a proof of concept, we showed that Epac1-camps is expressed in many neurons and reports [cAMP]i levels and their fluctuations in μM range after various physiological stimuli. We observed that membrane depolarisation and calcium release from internal stores produced [cAMP]i increases that led to further enhancement of calcium entry or calcium release through PKA-dependent phosphorylation. The effects were observed after relatively long-lasting calcium increases similar to those evoked by the tetanic stimulation commonly used to induce a long-term potentiation . The concerted actions of the two second messengers can convey specific messages between neurons but Ca2+ and cAMP should have different spatial and temporal ranges of actions. For example, the production and consumption rates of cAMP as well as its diffusion coefficient (Nikolaev et al. 2004) are more consistent with slower and spatially extended changes, whereas the actions of Ca2+ must be faster and more localised. How the effects of these two second messengers are orchestrated, and how they influence the neuronal activity, represents a challenging task for future studies. We believe that neuronal specificity, optical stability and sensitivity of Epac1-camps-based sensor provide a solid platform for such examinations.
We here report the application of Epac1-camps to measure cAMP concentrations in mammalian neurons embedded in their three dimensional context in living tissue. The sensitivity and specificity of neuron-targeted Epac1-camps are characterized and the probe was applied to examine crosstalk between calcium and cAMP signalling in brainstem neurons.
All animals were housed, cared for and euthanized in accordance with the recommendations of the European Commission (No. L358, ISSN 0378-6978), and protocols approved by the Committee for Animal Research, Göttingen University. Organotypic culture slices were obtained as described previously . Briefly, we prepared 250 μm-thick brainstem slices following a conventional procedure to obtain so called 'rhythmic slices' [17, 20, 30, 37]. All slices contained typical anatomy markers as documented in the atlas of the brainstem .
After preparation slices were placed on support membranes (Millicell-CM Inserts, PICMORG50; Millipore). 1 ml medium added to let the surface of the slice be continuously exposed to the incubator gas mixture and the medium (50% MEM with Earle's salts, 25 mM Hepes, 6.5 mg/ml glucose, 25% horse serum, 25% Hanks solution buffered with 5 mM Tris and 4 mM NaHCO3, pH 7.3) changed every second day. During the experiments, each slice was fixed on a coverslip mounted in the recording chamber and was continuously superfused at 34°C with artificial cerebro-spinal fluid (ACSF). Under experimental conditions the bath solution was fully exchanged within 1 s for different periods of time ranging from 10 s (applications of 50 mM K+, 100 μM ATP) to 2 min (forskolin, IBMX, DDA, rolipram etc.). High-K+ solution was prepared by exchanging Na+in ACSF; all other testing solutions were produced by adding aliquots of corresponding stock solutions directly to ACSF at approx. 1000-fold dilution.
Neurons in slices were transduced with AAV-Epac1-camps which expressed the sensor under control of the strictly neuron-specific synapsin 1 gene promoter . 1 μl of AAV solution (≈ 1 × 109 viral genomes) was applied directly onto the slice surface. Epac1-camps expression reached a steady state two days after viral application and did not change afterwards. Each test in the study was repeated with at least four different preparations, 4 to 8 neurons in each image field were examined.
The optical recording system was based on a Zeiss Axioscope and the slices were viewed under a 10× (Achroplan, N. A. 0.10) or 40× objective (Achroplan, N. A. 0.8). Epac1-camps was excited at 430 nm by the light generated by LED (20 mW, Roithner Lasertechnik). CFP emission at 470 nm and FRET between CFP and YFP (emitted at 535 nm) were separated with Optosplit (BFI Optilas, Puchheim) using dichroic mirror at 495 nm and 470 ± 12 and 535 ± 15 nm filters, respectively. Bleaching of Epac1-camps was negligible, thus allowing us to record fluorescence for more than 60 min without substantial loss of the signal. Images were captured by a cooled CCD camera (ANDOR, Offenbach) and collected with ANDOR software (500 × 500 pixels at 12 bit resolution). Time-dependent changes were obtained offline using MetaMorph software (Princeton Instruments, USA). The data were collected in the regions of interest which encircled the soma of neurons.
The basal [cAMP]i was 0.09 ± 0.03 μM (n = 16), corresponding to the lower branch of the dose-response curve in Fig. 5B, and all [cAMP]i observed changes were well below 5 μM (Tables 1 and 2), indicating that the probe has an optimal dynamic range for imaging cAMP in neurons. From Eqs. (1) and (2) follows that [cAMP] is approximately proportional to the measured ratio for [cAMP] levels when they are below K d of the sensor (1.5 μM). Because this condition is not always fulfilled, presentation of [cAMP] changes in the ratio form may show erroneous kinetics.
Epac1-camps also was calibrated in vitro using previously described procedures . Briefly, HEK293 cells were transfected with Epac1-camps. 24 h after transfection, cells were washed three times and resuspended in 5 mM Tris, 2 mM EDTA, pH 7.4 buffer. After disruption with an Ultraturrax device for 40 s on ice and 20 min centrifugation at 80000 rpm, fluorescence emission spectra of the supernatant (excitation at 436 nm, emission range 460 – 550 nm) were measured with a fluorescence spectrometer LS50B (Perkin Elmer Life Sciences) before and after addition of cAMP and BrOMecAMP (Biolog Life Science Institute, Bremen, Germany) at various concentrations. The concentration of Epac1-camps protein was 50 nM. FRET/CFP ratios were calculated by dividing peak emission intensities at 524 nm (FRET) and 477 nm (CFP) and analyzed by Origin 6.1 (Origin Lab Corporation, Northampton, MA). The use of emission wavelengths with wider slits such as set up in an Optosplit gave similar ratios. The curves for BrOMecAMP in vivo and in vitro were identical and were close to the dose-response curve obtained for the sensor in slices (Fig. 5B). K d values in vivo and in vitro were 1.6 and 1.5 μM for BrOMecAMP, respectively, and 1.2 μM for cAMP.
For imaging of intracellular calcium, 3 μM fura-2/AM was added to ACSF as an aliquot of DMSO-based stock solution and the mixture was sonicated. Slices were incubated with the dye for 20 min at 37°C followed by a 30 min-long wash-out to allow deesterification of ester precursor. Fura-2 was excited at 350 and 380 nm, and the emission was collected at 535 nm. [Ca2+]i values were obtained as described previously .
During simultaneous calcium and cAMP imaging we made concerns on possible contamination of the recorded fura-2 signal by CFP fluorescence from Epac1-camps which can be also excited by UV . Fig. 5C shows the two pairs of Epac1-camps (470 and 535 nm emission at 430 nm excitation) and fura-2 signals (535 nm emission at 350 and 380 nm excitation), which were recorded during high-K+ stimulation. Imaging of cells which were not loaded with fura-2 showed that excitation of CFP at 380 nm produced the fluorescence at 535 nm which is only 10 ± 2% (n = 24) of that recorded at 430 nm excitation. This estimate agrees well with the excitation spectrum of Epac1-camps in vitro . The two lowermost traces in Fig. 5C show the effect of possible bleeding of Epac1-camps fluorescence into the fura-2 channel. Subtraction of 15% Epac1-camps signal (Ex 430 nm, Em 535 nm) taken as an upper limit for bleeding to fura-2 channel (Ex 380 nm, Em 535 nm) did not change the traces significantly.
artificial cerebro-spinal fluid
cytoplasmic free Ca2+
- cyan (yellow) fluorescent protein:
2'5'-dideoxyadenosine: Epac: cAMP-dependent exchange factor
fluorescence resonance energy transfer
N- [2-(p-bromocinnamylamino)ethyl]-5-isoquinoline sulfonamide hydrochloride
protein kinase A
The authors thank Dr. C. Ludwig for critical reading of the manuscript and helpful suggestions.
- Alberts B, Johnson A, Walter P, Lewis J, Raff M, Roberts K: Molecular Biology of the Cell. 2002, London, Taylor & Francis Group, 4Google Scholar
- Kononenko NI, Mironov SL: The effects of intracellular cAMP injection on the electric characteristics of snail neurons. Neurophysiology. 1980, 12: 485-492.Google Scholar
- Ooashi N, Futatsugi A, Yoshihara F, Mikoshiba K, Kamiguchi H: Cell adhesion molecules regulate Ca2+-mediated steering of growth cones via cyclic AMP and ryanodine receptor type 3. J Cell Biol. 2000, 170: 1159-1167. 10.1083/jcb.200503157.View ArticleGoogle Scholar
- Hoogland TM, Saggau P: Facilitation of L-type Ca2+- channels in dendritic spines by activation of beta2 adrenergic receptors. J Neurosci. 2004, 24: 8416-8427. 10.1523/JNEUROSCI.1677-04.2004.View ArticlePubMedGoogle Scholar
- Liu Z, Geng L, Li R, He X, Zheng JQ: Frequency modulation of synchronized Ca2+ spikes in cultured hippocampal networks through G-protein-coupled receptors. J Neurosci. 2003, 23: 4156-4163.PubMedGoogle Scholar
- West AE, Chen WG, Dalva MB, Dolmetsch RE, Kornhauser JM, Shaywitz AJ, Takasu MA, Tao X, Greenberg ME: Calcium regulation of neuronal gene expression. Proc Natl Acad Sci USA. 2001, 98: 11024-11031. 10.1073/pnas.191352298.PubMed CentralView ArticlePubMedGoogle Scholar
- Chang YC, Huang CC: Perinatal brain injury and regulation of transcription. Curr Opin Neurol. 2006, 19: 141-147. 10.1097/01.wco.0000218229.73678.a8.View ArticlePubMedGoogle Scholar
- Adams SR, Harootunian AT, Buechler YJ, Taylor SS, Tsien RY: Fluorescence ratio imaging of cyclicAMP in single cells. Nature. 1991, 349: 694-697. 10.1038/349694a0.View ArticlePubMedGoogle Scholar
- Zaccolo M, De Giorgi F, Cho CY, Feng L, Knapp T, Negulescu PA, Taylor SS, Tsien RY, Pozzan T: A genetically encoded, fluorescent indicator for cyclic AMP in living cells. Nat Cell Biol. 2000, 2: 25-29. 10.1038/71345.View ArticlePubMedGoogle Scholar
- Ponsioen B, Zhao J, Riedl J, Zwartkruis F, Krogt van der G, Zaccolo M, Moolenaar WH, Bos JL, Jalink K: Detecting cAMP-induced Epac activation by fluorescence resonance energy transfer: Epac as a novel cAMP indicator. EMBO Rep. 2004, 5: 1176-1180. 10.1038/sj.embor.7400290.PubMed CentralView ArticlePubMedGoogle Scholar
- DiPilato LM, Cheng X, Zhang J: Fluorescent indicators of cAMP and Epac activation reveal differential dynamics of cAMP signaling within discrete subcellular compartments. Proc Natl Acad Sci USA. 2004, 101: 16513-16518. 10.1073/pnas.0405973101.PubMed CentralView ArticlePubMedGoogle Scholar
- Nikolaev VO, Bünemann M, Hein L, Hannawacker A, Lohse MJ: Novel single chain cAMP sensors for receptor-induced signal propagation. J Biol Chem. 2004, 279: 37215-37218. 10.1074/jbc.C400302200.View ArticlePubMedGoogle Scholar
- Washbourne P, McAllister AK: Techniques for gene transfer into neurons. Curr Opin Neurobiol. 2002, 12: 566-573. 10.1016/S0959-4388(02)00365-3.View ArticlePubMedGoogle Scholar
- Murphy RC, Messer A: Gene transfer methods for CNS organotypic cultures: a comparison of three nonviral methods. Mol Ther. 2001, 3: 113-121. 10.1006/mthe.2000.0235.View ArticlePubMedGoogle Scholar
- Kügler S, Kilic E, Bähr M: Human synapsin 1 gene promoter confers highly neuron-specific long-term transgene expression from an adenoviral vector in the adult rat brain depending on the transduced area. Gene Ther. 2003, 10: 337-247. 10.1038/sj.gt.3301905.View ArticlePubMedGoogle Scholar
- Hartelt N, Skorova E, Suhr M, Manzke T, Mironova L, Kügler S, Mironov SL: Imaging of respiratory network topology in living brain slices. Mol Cell Neurosci. 2008, 37: 425-431. 10.1016/j.mcn.2007.10.011.View ArticlePubMedGoogle Scholar
- Richter DW, Mironov SL, Büsselberg D, Bischof AM, Lalley PM: Respiratory rhythm generation: Plasticity of a neuronal network. Neuroscientist. 2000, 6: 188-205. 10.1177/107385840000600309.View ArticleGoogle Scholar
- Feldman JL, Del Negro CA: Looking for inspiration: new perspectives on respiratory rhythm. Nat Rev Neurosci. 2006, 7: 232-242. 10.1038/nrn1871.PubMed CentralView ArticlePubMedGoogle Scholar
- Ruangkittisakul A, Schwarzacher SW, Secchia L, Poon BY, Ma Y: High sensitivity to neuromodulator-activated signaling pathways at physiological [K+] of confocally imaged respiratory center neurons in on-line-calibrated newborn rat brainstem slices. J Neurosci. 2006, 26: 11870-11880. 10.1523/JNEUROSCI.3357-06.2006.View ArticlePubMedGoogle Scholar
- Mironov SL: Metabotropic glutamate receptors activate dendritic calcium waves and TRPM channels which drive rhythmic respiratory patterns in mice. J Physiol. 2008, 586 (9): 2272-2291. 10.1113/jphysiol.2007.149021.View ArticleGoogle Scholar
- Willoughby D, Cooper DM: Organization and Ca2+ regulation of adenylyl cyclases in cAMP microdomains. Physiol Rev. 2007, 87: 965-1010. 10.1152/physrev.00049.2006.View ArticlePubMedGoogle Scholar
- de Boer J, Philpott AJ, van Amsterdam RG, Shahid M, Zaagsma J: Human bronchial cyclic nucleotide phosphodiesterase isoenzymes: biochemical and pharmacological analysis using selective inhibitors. Br J Pharmacol. 1992, 106: 1028-1034.PubMed CentralView ArticlePubMedGoogle Scholar
- Nikolaev VO, Bünemann M, Schmitteckert E, Lohse MJ, Engelhardt S: Cyclic AMP imaging in adult cardiac myocytes reveals far-reaching beta1-adrenergic but locally confined beta2-adrenergic receptor-mediated signaling. Circ Res. 2006, 99: 1084-1091. 10.1161/01.RES.0000250046.69918.d5.View ArticlePubMedGoogle Scholar
- Huang YY, Kandel ER: Postsynaptic induction and PKA-dependent expression of LTP in the lateral amygdala. Neuron. 1998, 21: 169-178. 10.1016/S0896-6273(00)80524-3.View ArticlePubMedGoogle Scholar
- Harbeck MC, Chepurny O, Nikolaev VO, Lohse MJ, Holz GG: Simultaneous optical measurements of cytosolic Ca2+ and cAMP in single cells. Sci STKE. 2006, pl6-10.1126/stke.3532006pl6. 353
- Mironov SL: Metabotropic ATP receptor in hippocampal and thalamic neurones: pharmacology and modulation of Ca2+ mobilizing mechanisms. Neuropharmacology. 1994, 33: 1-13. 10.1016/0028-3908(94)90091-4.View ArticlePubMedGoogle Scholar
- Mironov SL, Richter DW: L-type Ca2+ channels in inspiratory neurones of mice and their modulation by hypoxia. J Physiol. 1998, 512: 75-87. 10.1111/j.1469-7793.1998.075bf.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Landa LR, Harbeck M, Kaihara K, Chepurny O, Kitiphongspattana K, Graf O, Nikolaev VO, Lohse MJ, Holz GG, Roe MW: Interplay of Ca2+ and cAMP signaling in the insulin-secreting MIN6 beta-cell line. J Biol Chem. 2005, 280: 31294-31302. 10.1074/jbc.M505657200.PubMed CentralView ArticlePubMedGoogle Scholar
- Fabiato A, Fabiato F: Calcium and cardiac excitation-contraction coupling. Annu Rev Physiol. 41: 473-484. 10.1146/annurev.ph.41.030179.002353.
- Shakiryanova D, Levitan ES: Prolonged presynaptic posttetanic cyclic GMP signaling in Drosophila motoneurons. Proc Natl Acad Sci USA. 2008, 105: 13610-13613. 10.1073/pnas.0802131105.PubMed CentralView ArticlePubMedGoogle Scholar
- Mironov SL, Langohr K: Modulation of synaptic and channel activities in the respiratory network of the mice by NO/cGMP signalling pathways. Brain Res. 2007, 1130: 73-82. 10.1016/j.brainres.2006.09.114.View ArticlePubMedGoogle Scholar
- Borodinsky LN, Spitzer NC: Second messenger pas de deux: the coordinated dance between calcium and cAMP. Sci STKE. 2006, pe22-10.1126/stke.3362006pe22. 336
- Mironova LA, Mironov SL: Approximate analytical time-dependent solutions to describe large-amplitude local calcium transients in the presence of buffers. Biophys J. 2008, 94: 349-358. 10.1529/biophysj.107.113340.PubMed CentralView ArticlePubMedGoogle Scholar
- Terrin A, Di Benedetto G, Pertegato V, Cheung YF, Baillie G, Lynch MJ, Elvassore N, Prinz A, Herberg FW, Houslay MD, Zaccolo M: PGE(1) stimulation of HEK293 cells generates multiple contiguous domains with different [cAMP]: role of compartmentalized phosphodiesterases. J Cell Biol. 2006, 175: 441-451. 10.1083/jcb.200605050.PubMed CentralView ArticlePubMedGoogle Scholar
- Dunn TA, Feller MB: Imaging second messenger dynamics in developing neural circuits. Dev Neurobiol. 2008, 68: 835-844. 10.1002/dneu.20619.PubMed CentralView ArticlePubMedGoogle Scholar
- Kim JW, Roberts CD, Berg SA, Caicedo A, Roper SD: Imaging cyclic AMP changes in pancreatic islets of transgenic reporter mice. PLoS ONE. 2008, 3: e2127-10.1371/journal.pone.0002127.PubMed CentralView ArticlePubMedGoogle Scholar
- Lissandron V, Rossetto MG, Erbguth K, Fiala A, Daga A: Transgenic fruit-flies expressing a FRET-based sensor for in vivo imaging of cAMP dynamics. Cell Signal. 2007, 19: 2296-2303. 10.1016/j.cellsig.2007.07.004.View ArticlePubMedGoogle Scholar
- Teschemacher AG, Paton JF, Kasparov S: Imaging living central neurones using viral gene transfer. Adv Drug Deliv Rev. 2005, 57: 79-93. 10.1016/j.addr.2004.05.004.View ArticlePubMedGoogle Scholar
- Kang G, Joseph JW, Chepurny OG, Monaco M, Wheeler MB, Bos JL, Schwede F, Genieser HG, Holz GG: Epac-selective cAMP analog 8-pCPT-2'-O-Me-cAMP as a stimulus for Ca2+-induced Ca2+ release and exocytosis in pancreatic beta-cells. J Biol Chem. 2003, 278: 8279-8285. 10.1074/jbc.M211682200.PubMed CentralView ArticlePubMedGoogle Scholar
- Mironov SL, Langohr K: Mechanisms of Na+ and Ca2+ influx into respiratory neurons during hypoxia. Neuropharmacology. 2005, 48: 1056-1065. 10.1016/j.neuropharm.2005.01.021.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.