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Comparative analysis of deutocerebral neuropils in Chilopoda (Myriapoda): implications for the evolution of the arthropod olfactory system and support for the Mandibulata concept

BMC Neuroscience volume 13, Article number: 1 (2012) Cite this article

Abstract

Background

Originating from a marine ancestor, the myriapods most likely invaded land independently of the hexapods. As these two evolutionary lineages conquered land in parallel but separately, we are interested in comparing the myriapod chemosensory system to that of hexapods to gain insights into possible adaptations for olfaction in air. Our study connects to a previous analysis of the brain and behavior of the chilopod (centipede) Scutigera coleoptrata in which we demonstrated that these animals do respond to volatile substances and analyzed the structure of their central olfactory pathway.

Results

Here, we examined the architecture of the deutocerebral brain areas (which process input from the antennae) in seven additional representatives of the Chilopoda, covering all major subtaxa, by histology, confocal laser-scan microscopy, and 3D reconstruction. We found that in all species that we studied the majority of antennal afferents target two separate neuropils, the olfactory lobe (chemosensory, composed of glomerular neuropil compartments) and the corpus lamellosum (mechanosensory). The numbers of olfactory glomeruli in the different chilopod taxa ranged from ca. 35 up to ca. 90 and the shape of the glomeruli ranged from spheroid across ovoid or drop-shape to elongate.

Conclusion

A split of the afferents from the (first) pair of antennae into separate chemosensory and mechanosensory components is also typical for Crustacea and Hexapoda, but this set of characters is absent in Chelicerata. We suggest that this character set strongly supports the Mandibulata hypothesis (Myriapoda + (Crustacea + Hexapoda)) as opposed to the Myriochelata concept (Myriapoda + Chelicerata). The evolutionary implications of our findings, particularly the plasticity of glomerular shape, are discussed.

Background

In arthropod phylogeny the emerging consensus is that Myriapoda are not to be considered the closest relatives of Hexapoda anymore (Tracheata concept), but rather that hexapods constitute a sister group or even an in-group of Crustacea (Tetraconata concept; e.g. [1–4]). Hence, it seems well established that from a marine ancestor of Euarthropoda, members of the Chelicerata as well as the Myriapoda and Hexapoda invaded land independently from each other [5, 6]. The successful transition from marine to terrestrial life requires a number of physiological adaptations that are important for survival out of water. The sensory organs of terrestrial species must be able to function in air rather than in water and hence were exposed to new selection pressures that may have reshaped the nervous system (see e.g. [7–10] for examples on terrestrial Crustacea). We are interested in how the structure of the central nervous system mirrors functional adaptations of the olfactory system to a terrestrial life style. Studying the olfactory system in Myriapoda and comparing it to that of Hexapoda may provide insights into how the arthropod nervous system evolved in response to new environmental and ecological challenges.

The Chilopoda together with the Progoneata (Symphyla + (Diplopoda + Pauropoda)) constitute the taxon Myriapoda. The position of monophyletic Myriapoda within the Euarthropoda is still under debate and most of the recent phylogenetic studies either place them as sister group to the Tetraconata (Crustacea + Hexapoda) together forming the taxon Mandibulata (e.g. [11, 12]) or as a sister group to the Chelicerata to form the taxon Myriochelata (e.g. [13]). The Chilopoda are one of the few arthropod taxa of which the internal phylogeny appears to be widely accepted [14]. The Notostigmophora (Scutigeromorpha) (Figure 1A) are the sister group to the Pleurostigmophora which are composed of Lithobiomorpha (Figure 2A) and Phylactometria. In the latter taxon, the Craterostigmomorpha (Figure 3A) are the sistergroup to the Epimorpha which are composed of Scolopendromorpha (Figure 4A, G) and Geophilomorpha (Figure 5A) [14].

Our knowledge of the chilopod nervous system largely relies on studies from the 19th and early 20th century using paraffin sections and light microscopy (e.g. [15–20]). Studies with contemporary neuroanatomical methods are only available for the brain, and specifically for the deutocerebrum (the second brain neuromere) of Scutigera coleoptrata [21].

The deutocerebrum in the mandibulate (Myriapoda + (Crustacea + Hexapoda)) brain is associated with the first pair of antennae and is characterized by a unified architecture: it comprises a paired anterior olfactory lobe that receives the chemosensory afferents from the first antennae, and (at least) a paired posterior neuropil [21, 22]. These uni- or bipartite posterior neuropils are thought to process mechanosensory stimuli and have a range of different names within the mandibulate taxa: antennal mechanosensory and motor center (AMMC) or dorsal lobe in Hexapoda (e.g. [23]), corpus lamellosum in Chilopoda [19–21, 24] and lateral antennular neuropil (LAN) plus median antennular neuropil (MAN) in malacostracan Crustacea and Remipedia [8, 25–29]. All of these structures can be unified under the term mechanosensory neuropils.

The chemosensory olfactory lobe (called antennal lobe in Hexapoda) is composed of structural and functional subunits [22], which are called olfactory glomeruli (or olfactory neuropils) [21, 24]. These subunits are clearly demarcated dense neuropils in which the axons of olfactory sensory neurons (OSN) terminate and interact with olfactory interneurons via the first synapses of the olfactory pathway [22, 30]. Thus, within the olfactory glomeruli of Hexapoda, malacostracan Crustacea and the House Centipede Scutigera coleoptrata, first order integration of olfactory input takes place, which is then relayed to secondary brain centers via olfactory projection neurons (e.g. [8, 21, 22]). The glomerular array in hexapods is thought to represent a chemotopic map, which forms the basis of the olfactory code [31–33]. Based on this uniform architecture and several additional synapomorphic characters [22], the olfactory system in general as well as the olfactory glomeruli in particular were suggested to represent homologous structures within the deutocerebrum of the Mandibulata [21, 22], whereas previously, also a convergent evolution was proposed [3].

Nevertheless, previous studies have revealed a high degree of plasticity in the shape and arrangement of mandibulate olfactory glomeruli, suggesting a critical evaluation of glomerular neuropils. In Scutigera coleoptrata, the olfactory glomeruli are elongated and arranged in parallel [21]. On the contrary, in many decapod Crustacea the olfactory lobes consist of glomeruli that are cone-like and in the lobe are arranged with their apices pointing inwards (reviews: [22, 27, 28, 34]). In some decapods crustaceans, these glomeruli may be extremely elongated [8, 10], whereas studies on representatives of the basal malacostracan taxon Nebalia (Leptostraca) suggest spherical glomeruli to be part of the malacostracan ground pattern (Kenning and Harzsch; unpublished results). Such spherical glomeruli are also present in marine Isopoda [9]. Furthermore, it has been well documented from crayfish (Astacidea), spiny lobsters (Palinuroidea) and hermit crabs (Paguroidea) that in the olfactory lobe each glomerulus is stratified and provides an outer cap, a subcap, and a base [8, 10, 34]. Most pterygote insects also feature spherical glomeruli [22], whereas primarily flightless hexapods diverge from this pattern [35, 36].

Is the shape of olfactory glomeruli of purely functional significance or does it contain an unexplored phylogenetic signal? Clearly, comparative information on the deutocerebral neuropils in a broad range of myriapods will contribute to this question. This study sets out to analyze the architecture of the central olfactory pathway in Chilopoda in more detail. To that end we analyzed the brains of representatives of eight chilopod species using histology, confocal laser scanning microscopy (cLSM), and 3D reconstruction. Our data are compared and evaluated with regard to the evolution of glomerular shape in Mandibulata.

Results

General morphology of the chilopod brain

Due to the anteriorly projecting antennae in the Chilopoda, the deutocerebrum (DC) is the most anterior part of the brain with regard to the body axis, so that the protocerebrum is always located dorsally and extends into lateral lobes where the optic neuropils are located (Figure 1, 2, 3, 4, 5). In the blind Cryptopidae (Scolopendromorpha) (Figure 4G), these lateral lobes are much smaller and are even totally reduced in the Geophilomorpha (Figure 5C, F). Contrary to Fahlander [20], who stated that a distinct midline neuropil is lacking in Geophilomorpha, all investigated representatives exhibit an unpaired midline neuropil (Figure 6). In S. coleoptrata and C. tasmanianus this unpaired neuropil is associated with small lateral lobes. As this study focuses on the organization of deutocerebral neuropils, we will not further consider here, if these neuropils represent an equivalent of the crustacean and hexapod central bodies or the chelicerate arcuate bodies (compare [3, 37]).

The morphology of the sensory antennal nerve differs in investigated chilopod species: while Scutigeromorpha, Lithobiomorpha and Craterostigmomorpha exhibit a "solitary" and robust antennal nerve, in Scolopendromorpha and Geophilomorpha it is composed of a bundle of several discrete nerves (Figure 4E, H; 5C, D, F; 6). Both types of antennal nerves enter the DC at its frontolateral or frontal edges (compare review [38]). The motoric antennal nerve will not further considered here. In the following the sensory antennal nerve is referred as antennal nerve.

Apart from the Geophilomorpha, the anterior part of the deutocerebrum is separated into two discrete hemispheres (Figure 1D, 2F, 3F, 4E, H; 5C, F). Anterograde backfilling experiments reveal that the antennal nerve targets the deutocerebral neuropils, which therefore are first order processing areas in the brain. In all Chilopoda examined, each deutocerebral hemisphere contains an olfactory lobe (OL) being composed of densely packed olfactory glomeruli (OG) and a corpus lamellosum (CL). A central coarse neuropil in the OL is not present in most examined taxa (uncertain for the Geophilomorpha). In the following, bilaterally paired structures will be referred in singular. A demarcation between deutocerebrum and tritocerebrum is not clearly apparent, although the stomodeal bridge and frontal connectives indicate the anterior margin of the tritocerebrum (compare [21, 24, 38])

Scutigeromorpha

The organization of deutocerebral neuropils in Scutigera coleoptrata (Figure 1A) was described in detail by Sombke et al. [21] and therefore will be only briefly reviewed here. Due to the roundish head outline, the shape of the brain differs from that of the pleurostigmophoran chilopod taxa. The antennal nerve enters the brain at its frontolateral edge (Figure 1D) and divides into two branches: an anterior part innervates the olfactory glomeruli whereas the posterior part innervates the corpus lamellosum. In S. coleoptrata, the olfactory lobes are arranged in an angle of nearly 180° to each other (Figure 1D, 6). Histological sections and dextran-biotin backfills reveals that single olfactory glomeruli have an elongated shape and are arranged in a parallel array (Figure 1B, D; and [21]). A 3D reconstruction (Figure 1D) reveals the bilateral symmetrical pattern with two contralaterally connected glomeruli (anterior deutocerebral commissure sensu Fahlander [20]) (Figure 1C, D: clc). In all histological section series and autofluorescence preparations, a total number of 34 distinct and uniquely identifiable OG in a more or less invariant arrangement is present (Figure 1D) [21]. The posterior part of the antennal nerve innervates the presumed mechanosensory neuropil called corpus lamellosum (CL; [19, 21, 24]), which in S. coleoptrata is composed of approximately eight parallel neuropilar lamellae [21]. Two different types of lamellae were recognized: the outer lamellae forming a distal connection, and inner lamellae that extend further dorsomedially to project towards the contralateral hemisphere (posterior deutocerebral commissure sensu Fahlander [20, 21]). Golgi impregnations shows that axons targeting the CL are much thicker than those targeting the OG and give off short side branches alongside their length [21]. In backfills of the antennal nerve, we found that the dye was also transported along thicker neurites projecting into the ventrolateral protocerebrum and the subesophageal ganglion (Figure 1C) [21].

Figure 1
figure 1

Scutigeromorpha. A Scutigera coleoptrata. B Single optical section of a neurobiotin backfill showing an olfactory lobe with distinct olfactory glomeruli. cLSM scan. C cLSM scan (maximal projection) of the brain and the subesophageal ganglion. View from ventral. Left antennal nerve was filled with neurobiotin. Antennal neurites project into the seg. D 3D reconstruction of the brain of S. coleoptrata with deutocerebral neuropils. Blue: olfactory glomeruli, yellow: corpus lamellosum. Abbreviations: cl corpus lamellosum, clc contralateral connection, fg frontal ganglion, na nervus antennalis, np neurite projections, ol olfactory lobe, pc protocerebrum, seg subesophageal ganglion. Scalebars: A = 10 mm, B, C = 100 μm.

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Lithobiomorpha

The lithobiomorph head is flattened, a fact that is mirrored in the shape of the brain (reviewed in [24]). The antennal nerve enters the deutocerebrum at its frontal edge (Figure 2B-G). The deutocerebrum is organized in an anterior olfactory lobe (OL) with glomeruli (OG) and a posterior corpus lamellosum (CL) (Figure 2B-G). In contrast to S. coleoptrata, the OLs extend in a slightly dorsomedian direction resulting in an angle of nearly 90° (Figure 2G, 6). Antennal afferents were revealed by neurobiotin backfills which, in addition to the terminations in the OL and CL, also show a bundle of neurites projecting from the antennal nerve through the tritocerebrum deep into the subesophageal ganglion (Figure 2C, F: np). Within the OL, two OG feature a contralateral connection (Figure 2C, F: clc, D: arrow). Histological sections and neurobiotin backfills reveal that single OG have a drop-like to elongated shape that narrows to their anterodistal edges (Figure 2D, G). All OG appear compact without any subcompartments. The 3D reconstruction reveals a bilaterally symmetrical pattern with a total number of 43 OG (Figure 2G). The CL is located posteriorly to the OG and extends a small contralateral connection (not shown). The neuropil is composed of at least four lamellae (Figure 2C, E asterisks). However, the lamellae are more densely packed than in S. coleoptrata so that a precise count is not possible.

Figure 2
figure 2

Lithobiomorpha. A Lithobius forficatus. B Histological horizontal section of the head showing the deutocerebral lobes with olfactory glomeruli and corpus lamellosum (dashed line) as well as the ommatidia. C Voltexrendering (Amira) of a neurobiotin backfill showing deutocerebral neuropils and neurite projections. D Horizontal optical section (cLSM scan) of a neurobiotin backfill (dorsal deutocerebrum) showing single olfactory glomeruli (asterisks). The arrow points to the contralateral connection of the olfactory lobe. E Horizontal optical section (cLSM scan) of a neurobiotin backfill (ventral deutocerebrum) showing the corpus lamellosum. Asterisks mark single lamellae of the neuropil. F Voltexrendering (Amira) of a neurobiotin backfill showing the brain with deutocerebral neuropils and projections. Arrows point to antennal neurites projecting into the subesophageal ganglion. G 3D reconstruction of the brain with deutocerebral neuropils. Blue = olfactory glomeruli, yellow = corpus lamellosum. Contralateral connection of OG and CL is not shown. Abbreviations: an antenna, cl corpus lamellosum, clc contralateral connection, dc deutocerebrum, fg frontal ganglion, na nervus antennalis, no nervus opticus, np neurite projections, ol olfactory lobe, om ommatidia, pc protocerebrum, seg subesophageal ganglion. Scalebars: A = 10 mm, B = 500 μm, C-E = 100 μm.

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Craterostigmomorpha

This is the first investigation of the nervous system of Craterostigmus tasmanianus (Figure 3A). Like in the Lithobiomorpha, the head of the Craterostigmomorpha is flattened, which is reflected in the shape of the brain (Figure 3F). The robust antennal nerve enters the deutocerebrum at its frontal edge (Figure 3B, F). In principle, the deutocerebrum is organized in an anteromedian OL and a posterior CL (Figure 3B-F). The OLs extend in a median direction resulting in an angle of nearly 90° (Figure 3F, 6). Histological sections and autofluorescence preparations reveal that single OG have a drop-like to elongated shape with a nearly circular profile and a smaller diameter distally (Figure 3B, E, F). The OG are arranged in an anteroposterior direction (Figure 3B, F). A contralateral connection of the OLs was not found. The 3D reconstruction shows a total number of 36 OG (Figure 3F). The CL is located posteriorly to the OL and features a thin contralateral connection (not illustrated). Due to the fixation, a lamellar organization was not clearly recognizable. However, a partition into discrete lamellae is likely (Figure 3C, D arrow).

Figure 3
figure 3

Craterostigmomorpha. A Head and maxillipedes of Craterostigmus tasmanianus from dorsal. B Horizontal optical section of an autofluorescence preparation (cLSM stack). Single olfactory glomeruli (asterisks) are weakly detectable. C Different horizontal section of the same preparation as in B. The arrow marks the structural composition of the corpus lamellosum. D Histological cross section of the right brain hemisphere showing the proto- and deutocerebrum with the corpus lamellosum (dashed line). E Histological cross section of the left deutocerebrum showing the dorsomedian located olfactory glomeruli. F 3D reconstruction of the brain with deutocerebral neuropils. Blue = olfactory glomeruli, yellow = corpus lamellosum. Contralateral connection of the CL is not shown. Abbreviations: an antenna, cl corpus lamellosum, dc deutocerebrum, ey eye, mp maxillipede, na nervus antennalis, ol olfactory lobe, pc protocerebrum. Scalebars: A = 1 mm, B-E = 100 μm.

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Scolopendromorpha

Similar to the Lithobiomorpha and Craterostigmomorpha, the head and also the brain of the Scolopendromorpha are flattened. The DC is innervated by several antennal nerve bundles (Figure 4E, H) at its frontal edge. The DC is composed of an anteriorly located olfactory lobe and a posteriorly located CL (Figure 4B-E, H, I). The OLs extend in a slightly dorsomedian direction resulting in an angle of less than 90° (Figure 4B, E, H, I). The OG are arranged in an anteroposterior direction. In the three investigated scolopendromorph species, the shape of the OG appears spheroid to drop-like elongated (Figure 4B-F, H-J). In Scolopendra oraniensis and Cryptops hortensis, ventral glomeruli are much bigger (Figure 4C, F, H, J). In S. oraniensis, three enlarged ventral OG are present (Figure 4F) while in C. hortensis two enlarged ventral OG exist (Figure 4J). A contralateral connection of the OLs is absent. Based on histological sections, backfill experiments, and autofluorescence preparations, all OG appear compact without any subcompartments and are arranged in a bilaterally symmetrical pattern. Numbers of OG range from 51 in Scolopendra subspinipes (Figure 4E), across 56 in Cryptops hortensis (Figure 4H) to 58 in Scolopendra oraniensis (Figure 4F). The CL is located posteroventrally to the OL. A contralateral connection of the CL is always present, although it varies in thickness in the three investigates species. In Scolopendra subspinipes, it appears as a thick connection (Figure 4E) while in Cryptops hortensis it appears very thin (not shown in Figure 4I). Single lamellae are not clearly detectable. However, backfill experiments reveal an alternating texture within the neuropil (Figure 4B, C arrow, G). Neurobiotin backfills reveal an additional neurite bundle projecting from the antennal nerve through the tritocerebrum into the subesophageal ganglion (Figure 4B, C, H: np).

Figure 4
figure 4

Scolopendromorpha. A Scolopendra oraniensis. B Neurobiotin backfill of the antennal nerve in S. oraniensis showing the olfactory lobe, the corpus lamellosum, and neurite projections (horizontal maximal projection, cLSM scan). C Single optical horizontal section of a Lucifer yellow backfill in S. oraniensis (cLSM scan). Antennal neurites cross each other in a sorting zone and project into different neuropils. The arrow marks the structural composition of the corpus lamellosum in which single lamellae are weakly noticeable. The large ventral OG is visible in this section. Single olfactory glomeruli in the olfactory lobe are arranged like in a grape. D Neurobiotin backfill of the antennal nerve of S. oraniensis. Only a subpopulation of the antennal neurites and olfactory glomeruli is labeled (horizontal maximal projection, cLSM scan). E 3D reconstruction of the brain of Scolopendra subspinipes (dorsal protocerebrum is not shown) with deutocerebral neuropils. Blue = olfactory glomeruli, yellow = corpus lamellosum. F 3D reconstruction of deutocerebral neuropils of Scolopendra oraniensis combined with volume rendering of the antennal backfill in B. Three enlarged ventral glomeruli (I, II, III) are present. G Cryptops hortensis. H Single horizontal optical sections (cLSM) of a neurobiotin backfill of the right antennal nerve in C. hortensis from dorsal to ventral. Antennal nerve bundles and innervation of single olfactory glomeruli. H' Sorting zone (arrow) of antennal neurites and corpus lamellosum. H'' Larger ventral olfactory glomerulus (arrow) and neurite projections. I 3D reconstruction of the brain of C. hortensis with deutocerebral neuropils and midline neuropil. Contralateral connection of the CL is not shown. Blue = olfactory glomeruli, yellow = corpus lamellosum, red = midline neuropil. J Lateral view of the 3D reconstruction in I. Two enlarged ventral glomeruli (I, II) are present. Abbreviations: cec circumesophageal connectives, cl corpus lamellosum, mn midline neuropil, na nervus antennalis, np neurite projections, ol olfactory lobe, pc protocerebrum, pcg protocerebral gland, seg subesophageal ganglion, sz sorting zone, vog ventral olfactory glomerulus. Scalebars: A and F = 10 mm, B-D, G = 100 μm.

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Geophilomorpha

The brain of obligatory blind Geophilomorpha is spherical in shape and also the most modified within the Chilopoda [24, 38]. The dominant component of the geophilomorph brain is the deutocerebrum. A clear demarcation between proto- and deutocerebrum is not detectable (Figure 5C, F). The antennal nerve comprises 10-15 bundles of sensory neurons and innervates the DC at its frontal edge (Figure 5B-F). The deutocerebral hemispheres are fused posteromedially (Figure 5B-F). The OLs are arranged more or less parallel to each other (Figure 5B, C, F). In both investigated species, the OL is composed of spheroid to slightly ovoid OG. In Stigmatogaster dimidiatus, the OL appears slightly invaginated posteriorly (Figure 5D, E: arrow) thus resulting in a cup-like shape. The number of OG is 49 in Haplophilus subterraneus and 97 in Stigmatogaster dimidiatus. A conspicuous contralateral connection (clc) features the OLs of S. dimidiatus, where two elongated OG extend a thin clc (Figure 5E: arrow, F: asterisks). However, in H. subterraneus, a clc of the OLs is not present. The OG appear compact without any subcompartments and a bilateral symmetry seems to be present (Figure 5B-F). In both investigated species, an enlarged ventral glomerulus is found (Figure 5F: vog; not shown in the reconstruction of H. subterraneus). The CL is located posteroventrally to the OL (Figure 5B-F) and features a thin contralateral connection (Figure 5F). In the autofluorescence preparations, this thin connection could not be depicted clearly. In most of the preparations, the CL appears lamellar (Figure 5B, D inset; arrows). Posteriorly directed antennal neurite projections were revealed by neurobiotin backfills, which showed a bundle of neurites projecting from the antennal nerve, through the tritocerebrum (asterisk in inset Figure 5D) and probably into the subesophageal ganglion via the circumesophageal connectives (Figure 5D: cec). Interestingly, several somata were filled by neurobiotin in the ventral brain (Figure 5D left: arrow) branching intensively in an anteroposterior direction. Whether these somata belong to projection- or local interneurons remains unknown.

Figure 5
figure 5

Geophilomorpha. A Geophilus carpophagus. B Single horizontal optical section (cLSM) of an autofluorescence preparation of the brain of Haplophilus subterraneus showing olfactory glomeruli (asterisks) and the structural composition of the corpus lamellosum (arrow). C 3D reconstruction of the brain of H. subterraneus with deutocerebral neuropils and midline neuropil. Blue = olfactory glomeruli, yellow = corpus lamellosum, red = midline neuropil. Contralateral connection of the CL is not shown. D Single horizontal optical sections (cLSM) of a neurobiotin backfill of the right antennal nerve in Stigmatogaster dimidiatus from ventral to dorsal. Left: several somata stained by the neurobiotin backfill (arrow) and neurite projections into a circumesophageal connective. Middle: Antennal nerves and olfactory glomeruli. Right: The slightly concave appearance of the olfactory lobe. Inset: Sagittal optical section of the same preparation showing the structural composition of the corpus lamellosum (arrow) and neurite projections (asterisk). E Single horizontal optical sections (cLSM) of an autofluorescence preparation of the brain of S. dimidiatus from dorsal to ventral. Olfactory glomeruli (asterisks) and the contralateral connection between the posteroventral OG (arrow). E' Concave appearance of the olfactory lobe (arrow). E'' ventrolateral position of the corpus lamellosum. F 3D reconstruction of the brain of S. dimidiatus with deutocerebral neuropils and midline neuropil. Blue = olfactory glomeruli, gray = bigger ventral olfactory glomerulus, yellow = corpus lamellosum, red = midline neuropil. Abbreviations: cec circumesophageal connective, cl corpus lamellosum, mn midline neuropil, na nervus antennalis, np neurite projections, ol olfactory lobe, pc protocerebrum, vog ventral olfactory glomerulus. Scalebars: A = 5 mm, B, D, E = 100 μm.

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Discussion

Chilopoda: the antennal nerve innervates two separate deutocerebral neuropils

Supporting the descriptions of Seifert [38] and Fahlander [20], we found that a sensory antennal nerve composed of several discrete bundles (as opposed to one "solitary" nerve) occurs only in representatives of the Scolopendromorpha and Geophilomorpha. In a phylogenetic view, this feature can be regarded as an apomorphy for the taxon Epimorpha.

Fahlander [20] described the nervous system of various Chilopoda and also interpreted the results from Saint-Rémy [15] and Hörberg [19] in a broad comparative study. Although previous authors mentioned a glomerular organized antennal lobe for the Chilopoda (e.g. [17–20, 39]), the number, organization, and structural composition remained unclear. Sombke et al. [21] reinvestigated the deutocerebral neuropils in Scutigera coleoptrata using a variety of histological and immunhistochemical methods. Similar to S. coleoptrata [21], the deutocerebrum of the Chilopoda investigated here is organized into structured neuropils that can be divided into two different regions: olfactory lobe and corpus lamellosum (Figure 6, 7). Moreover, a similar organization of deutocerebral neuropils may be present in representatives of the Diplopoda ([40], Seefluth and Sombke unpublished data.) as well as in representatives of the Hexapoda and Crustacea [e.g. [23, 27]].

The chilopod olfactory lobes and olfactory glomeruli

In principle, the olfactory lobe (OL) extends from the entrance of the antennal nerve into the brain on towards the dorsomedian brain and is composed of olfactory glomeruli (OG) which are located in the anterior part of the deutocerebrum. The OG are innervated from the periphery. As a result of different innervation angles of the antennal nerves, the overall orientation of the olfactory lobes differs in the Chilopoda (Figure 6). In contrast to the remaining chilopod taxa, the OL of the Geophilomorpha appears globular and slightly invaginated (Figure 6, 7). The alignment of the OG also differs in investigated taxa: while in S. coleoptrata the OG form more or less parallel layers, the drop-like shape in L. forficatus, C. tasmanianus and the representatives of the Scolopendromorpha results in a more compact arrangement.

The presence of a central coarse neuropil in the OL of Geophilomorpha is uncertain. In many hexapod taxa, the glomeruli surround a coarse neuropil e.g. Dictyoptera [41], Hymenoptera [42], Lepidoptera and Diptera (reviewed in [22]). Contrary, in Archaeognatha the OL is composed of elongated OG without a central neuropil [36]. In malacostracan Crustacea, the OG are arranged in a peripheral radial array that surrounds a loose core of neuronal processes (reviewed in [22, 34]). Single glomeruli in these animals are also innervated from the periphery (reviews: [22, 27, 28, 34]).

In Scutigera coleoptrata, 34 individually identifiable OG per olfactory lobe were detected repeatedly in several specimens and these glomeruli form a fixed array, so that individual OG are identifiable [21]. In the present study, glomerular numbers were only determined in few specimens, so that the numbers have to be viewed with caution. Nevertheless, we speculate that the determined numbers are taxon-specific within the Chilopoda. In Lithobius forficatus, 43 OG were detected, and 36 in Craterostigmus tasmanianus. In the investigated Scolopendromorpha, the number of OG ranges around 50-60, while in the Geophilomorpha, some variation was encountered (49 in H. subterraneus, 97 in S. dimidiatus). In hexapods, the number of olfactory glomeruli ranges from about 20 in Collembola to approx. 250 in ants (reviewed in [22, 35]) and seems to be invariant within species (e.g. [42–52]). In Crustacea the number of OG varies from approximately 60 to 1300 (reviewed in [10, 22, 53]) but it is uncertain if crustaceans have a fixed set of OGs [53, 54].

The number of glomeruli is generally thought to provide a good indication regarding how many different olfactory receptor proteins (OR) are expressed in the antenna. One OSN typically expresses a single OR, and all OSNs expressing a specific receptor project their axons to the same glomerulus. Odor input thus paints a map of activation over the glomerular array.

The size of OG is more or less taxon-specific and constant within the investigated chilopods (Figure 7). The only exceptions are the ventrally located enlarged OG in Scolopendra oraniensis, Cryptops hortensis, and Stigmatogaster dimidiatus. This is also true for the posteriormost OGs in Scutigera coleoptrata (compare [21]). In general, glomeruli of increased fitness-related importance tend to increase in size. Sex-specific enlargement ("macroglomeruli") are known from various hexapods e.g. moths [55], cockroaches [56] or honeybees [42]. Other enlargements have been found to be associated with trail pheromones and with specific food cues [57]. In this study a functional correlation was not conducted, and no conclusions regarding the functional significance of macroglomeruli in Chilopoda can be drawn.

In histological sections, backfills, and autofluorescence preparations, there is not any evidence of further compartmentalization of the OG in the investigated Chilopoda as it is known from hexapods and malacostracan crustaceans. In honeybees (Hexapoda), olfactory glomeruli have a concentric organization [42, 58–62], where only the periphery is innervated by axons of sensory neurons. A longitudinal subdivision of the OG into cap, subcap, and base has been well documented in crayfish, clawed and clawless lobsters, and hermit crabs (Crustacea) [8, 10, 63–67].

The shape of the OG in the investigated chilopods displays a considerable plasticity (Figure 6, 7). OG in the Scutigeromorpha have an elongated shape and in some the distal end is thickened and/or bent posteriorly [21]. According to Fahlander [20], the internal organization of deutocerebral neuropils in Lithobius forficatus strongly resembles those of the Scutigeromorpha, but here we show that the shape of the OG actually differs. In Lithobius forficatus and Craterostigmus tasmanianus, the overall shape of OG ranges from elongated (more than two times longer than wide) to drop-shaped, with a smaller anterior diameter. In the Scolopendromorpha, the shape of OG is mostly drop-like to spheroid and in the Geophilomorpha, the OG have a spheroid shape. For the Chilopoda, it is unclear if elongated or spheroid glomeruli represent the ancestral shape of the OG in this group. Here, we take benefit from the fact that the debate on the internal phylogeny of Chilopoda rather unequivocally gravitated into accepting the Pleurostigmophora concept of Verhoeff [68] in the past four decades so that we can map our results on a stable phylogeny of Chilopoda. Based on the number and position of stigmata, this phylogenetic concept separates the Scutigeromorpha (= Notostigmophora) as sister group to all other Chilopoda (Pleurostigmophora). This phylogenetic concept has received substantial support from the analysis of morphological, molecular and combined morphological-molecular data sets (e.g. [14, 69–73]). If we accept this phylogeny, we have to assume that elongated OG mark the plesiomorphic state in Chilopoda, perhaps retained from the myriapod ground pattern. In this view, the exclusive occurrence of spheroid OG has to be considered an additional apomorphy of the Geophilomorpha. However, alternatives to this view are possible. In most pterygote Hexapoda, the antennal lobe (or olfactory lobe) is organized into numerous roughly spheroid OG. In Archaeognatha the OG have an elongated shape [36]. In Crustacea, the shape of olfactory glomeruli differs considerably (reviewed in [22]). The olfactory lobes of malacostracan Crustacea are typically composed of glomeruli which are columnar or wedge-shape, (reviews [3, 34]). or slightly cone-shaped [9, 74]. Nevertheless, certain Stomatopoda possess olfactory lobes with spheroid OG [75] as do Remipedia [29, 76], Leptostraca [18], and marine isopods [9]. Within several taxa of the Chelicerata, spheroid neuropil units have been reported but these are not associated with the second brain neuromere [77–82]. Along these lines it would appear that spheroid OG within the deutocerebrum characterize both, the ground patterns of Malacostraca and Hexapoda. If this hypothesis holds true, it is parsimonious to assume that spheroid glomeruli are also part of the ground pattern of Mandibulata and would thus characterize the ground pattern of Myriapoda. In this view, the elongate shape of OG in Scutigeromorpha would be a derived character characteristic for this particular group.

In this context, the question arises if the shape of olfactory glomeruli is of purely functional significance or if it does contain an unexplored phylogenetic signal. Clearly, considering glomerular shape alone is not sufficient to answer these questions. Concerning the central olfactory pathway of malacostracan crustaceans and hexapods, the fact that in both groups the afferents of chemosensory receptor neurons terminate in lobed deutocerebral neuropils where they target neuropil units to make synaptic contacts to local olfactory interneurons and olfactory projection neurons has been suggested as evidence that the olfactory system in these two taxa goes back to a shared ground pattern [22]. Furthermore, in both taxa the axons of olfactory projection neurons link the olfactory neuropils to secondary olfactory processing centers in the protocerebrum. What is more, local olfactory interneurons in both taxa include a characteristic innervation by one or very few serotonergic giant neurons that target every OG. Hence it seem legitimate to suggest that in the ground pattern of the common ancestor of hexapods and malacostracan crustaceans, a basal computational circuit was present that included the antennal afferents, local olfactory interneurons, and projection neurons (compare [22]). Taken together, this mosaic of architectural differences as well as similarities suggests that most likely the olfactory centers and their connections are homologous in hexapods and malacostracan crustaceans, having evolved in divergent directions from a much simpler ground pattern. What we do not know at the moment is to what level of detail the connection pattern of antennal afferents with olfactory local interneurons and projection neurons in Myriapoda resembles that of Tetraconata.

Figure 6
figure 6

Brains of selected Chilopoda. Schematic representation of the brains of selected Chilopoda with illustration of the olfactory glomeruli (blue), corpus lamellosum (yellow), Midline neuropil (red) and neurite projections (dashed lines). View from dorsal. The protocerebrum appears brighter. Abbreviations: cl corpus lamellosum, dc deutocerebrum, mn midline neuropil, np neurite projection, nr nervus recurrens, ol olfactory lobe, pc protocerebrum, pcg protocerebral gland, seg subesophageal ganglion, tc tritocerebrum.

Full size image

The corpus lamellosum

In a brief description of deutocerebral neuropils in Lithobius variegatus, Strausfeld et al. [39] described that the antennal nerve innervates the olfactory lobe and that a lateral strand projects to a region behind it, which the authors called dorsal lobe in analogy to the hexapod mechanosensory neuropil. This posterior deutocerebral neuropil in Chilopoda had already been termed "masse lamelleuse" by Saint-Rémy [16] and latinized by Fahlander [20] who called it corpus lamellosum (CL). Because it reflects the characteristics of this structured neuropil, we suggest maintaining this nomination. As mentioned above, in Scutigera coleoptrata the posterior partition of the antennal nerve innervates the CL in which approximately eight parallel lamellae were found [21]. Golgi impregnations showed that sensory neurites innervating the CL appear much thicker than those innervating the olfactory glomeruli and give off short side branches along their length [21]. Although similar Golgi experiments on other chilopod taxa have not been conducted yet, it appears to us that the architecture of the CL in the other chilopod taxa investigated here is similar to that of S. coleoptrata. In S. coleoptrata, the parallel lamellae project dorsomedially and extend into the posterior deutocerebral commissure [20, 21]. By backfilling the antennal nerve in Lithobius forficatus, at least 4 single lamellae are visible. The report of Fahlander [20] that in Lithobius forficatus the CL is not composed of distinct lamellae can therefore be rejected. In Craterostigmus tasmanianus, a lamellation is only partially visible. In the Scolopendromorpha, backfill experiments also show an arrangement of parallel fibers suggesting a lamellation in the investigated genera. In the Geophilomorpha, the CL also appears lamellar. Possibly, due to a higher degree of condensation, single lamellae could not be detected. In summary, all the investigated chilopods exhibit a CL, which is composed of parallel lamellae and features a contralateral connection.

In pterygote Hexapoda, the first and second antennomeres of the antenna supply the dorsal lobe (mechanosensory neuropil) whereas the flagellar sensilla are mostly specialized for olfactory perception and their neurites project into the olfactory lobe [56]. Examples where mechanosensory and gustatory afferents project into the such a mechanosensory neuropil in the posterior region of the deutocerebrum (and in some cases even proceed into the anterior subesophageal ganglion) are e.g. Periplaneta americana [62, 83], Apis mellifera [84], Gryllus bimaculatus [85, 86], and Aedes aegypti [33, 87]. In these organisms, presumptive tactile antennal afferents provide two pairs of long branches whereas several short branches are orientated laterally and form a multilayered arrangement medially in the dorsal lobe. This arrangement exhibits a similarity to the branching pattern of sensory axons in the CL of Scutigera coleoptrata [21]. In malacostracan crustaceans, the first (deutocerebral) pair of antennae, in addition to the aesthetasc chemosensory pathway, provides mechanosensory and non-aesthetasc chemosensory input to the lateral and median antennular neuropil (LAN and MAN) [25–28, 34]. Between the lobes of the LAN, contralateral connections occur in Decapoda. The general organization of the LAN and MAN in many respects matches the innervation and connections of the CL. To summarize, we suggest that in the ground pattern of Chilopoda, Hexapoda, and Crustacea, the posterior deutocerebrum is characterized by at least one neuropil (corpus lamellosum, dorsal lobe, lateral antennular neuropil) that processes mechanosensory input from the first pair of antennae. Such a neuropil is absent in Chelicerata and therefore represents a homology of Mandibulata (apomorphic character state). However, the architecture of this neuropil was then elaborated in different ways in the various mandibulate lineages.

Figure 7
figure 7

Deutocerebral neuropils of selected Chilopoda. Schematic representation of the deutocerebral neuropils in representatives of the Chilopoda (left hemisphere). Horizontal view with equal scaling.

Full size image

Posterior neurite projections

In all investigated Chilopoda (except for C. tasmanianus), antennal afferents also project into the subesophageal ganglion and even into the ventral nerve cord. These neurites project ipsilaterally and bypass the deutocerebral neuropils. In addition, in S. coleoptrata a second projection to the ventrolateral protocerebrum was found [21]. We speculate that these posterior neurites may project to a gustatory or motoric center in the subesophageal ganglion. In Hexapoda, certain neurites from the antennal nerve also project to the subesophageal ganglion and the thoracic ganglia [62, 88–90]. Barrozo et al. [90] suggested that these neurite projections with a characteristic larger diameter might serve to insure a rapid neuronal transmission of sensory inputs towards centers responsible for controlling motor activities and physiological processes. In Crustacea, these characteristic posterior neurite projections have not yet been described. As a consequence it can be assumed, that they are reduced within the Crustacea. The presence in Chilopoda and Hexapoda could indicate an additional shared feature of the mandibulate deutocerebrum. However, neurite projections are also described from the pectines in Scorpiones [81]. If these neurite projections correspond to those in Chilopoda and Hexapoda remains uncertain.

The deutocerebrum and olfactory lobes of Euarthropoda in an evolutionary context: support for the Mandibulata concept

In the Chilopoda, the deutocerebrum is characterized by two distinct neuropil regions, which are innervated by antennal sensory afferents. The olfactory glomeruli are bilaterally and symmetrically arranged and appear presumably in a taxon-specific fixed number. Contralateral connections occur in some species. The corpus lamellosum is a structured neuropil and exhibits a contralateral connection. In the Chilopoda, antennal neurite projections transit the deutocerebrum and project into the subesophageal ganglion.

In Chilopoda, Crustacea, and Hexapoda, distinct neuropils for processing sensory information of the (first) antennae are located in the deutocerebrum. According to Hox-gene expression patterns and morphological investigations, the deutocerebrum and the deutocerebral antennae are homologous within Mandibulata and correspond to the chelicere neuromere in Chelicerata [91–93]. Although in some Chelicerata glomerular chemosensory processing areas associated with a sensory appendage are located in the trunk ganglia (e.g. [81]), distinct neuropils for processing of chemo- and mechanosensory information have not yet been reported for their second brain neuromere. Moreover, a characteristic divergence of sensory neurites and the presence of a mechanosensory neuropil are not realized in Chelicerata. There is a consensus now that the antenna in Onychophora is a protocerebral appendage and therefore not equivalent to the deutocerebral antenna in Mandibulata [94–97]. In Onychophora, chemosensory centers composed of glomerular neuropils are located within the protocerebrum [98, 99]. Similar to the chelicerates, separate mechanosensory neuropils associated with the antennal input do not seem to be present in onychophorans. Strausfeld and co-workers [98, 99] emphasize the structural similarities of onychophoran and chelicerate brains so that we suggest that these two taxa represent the plesiomorphic arthropod character state concerning brain architecture. In summary, within the arthropod outgroups of Mandibulata, chemosensory appendages and olfactory glomeruli, if present, are never located in the second brain neuromere (deutocerebrum).

Conclusion

Our most important conclusion is that the presence of a bifunctional deutocerebrum composed of distinct neuropils for chemo- and mechanosensory qualities is homologous in Chilopoda, Diplopoda, Hexapoda and Crustacea and can therefore be postulated as an apomorphic character complex for the Mandibulata. However, our neuroanatomical data strongly contradict a sister group relationship of Myriapoda and Chelicerata ("Myriochelata; [13]), but instead support the Mandibulata concept (e.g. [11, 12]). In this view, the absence of olfactory lobes in various Crustacea (Branchiopoda and certain "Maxillopoda"; [22, 100, 101]) and Hexapoda (Odonata, certain Hemiptera and Coleoptera, reviewed in [22]) as well as the absence of the mechanosensory neuropils in Cephalocarida (Crustacea; [102]) can be interpreted as a reduction.

Methods

Experimental animals

Specimens were collected on the Balearic Island Ibiza (Spain) mainly in pine forests or in Germany mainly in litter and soil. Specimens of Craterostigmus tasmanianus were collected by Robert Mesibov in Tasmania. If not fixed directly after capture, individuals were kept in plastic tubes (50 ml; Carl Roth, Germany) at room temperature. For keeping of animals, they were transferred into plastic boxes supplied with bark and water. They were fed with Drosophila melanogaster or juveniles of Achaeta domestica.

Representatives of all five chilopod subtaxa were investigated: (1) Scutigera coleoptrata (Linnaeus, 1758), Scutigeromorpha: Scutigeridae; collected in Spain: Ibiza. (2) Lithobius forficatus (Linnaeus, 1758), Lithobiomorpha: Lithobiidae; collected in Germany: Aachen, Greifswald. (3) Craterostigmus tasmanianus Pocock, 1902, Craterostigmomorpha; collected in Australia: Tasmania. (4) Cryptops hortensis (Donovan, 1810), Scolopendromorpha: Cryptopidae; collected in Germany: Aachen, Greifswald. (5) Scolopendra oraniensis Lucas, 1846, Scolopendromorpha: Scolopendridae; collected in Spain: Ibiza. (6) Scolopendra subspinipes Leach, 1815, Scolopendromorpha: Scolopendridae; ordered from btbe Insektenzucht GmbH, Germany http://www.futtertiere24.de/. (7) Haplophilus subterraneus (Shaw, 1794), Geophilomorpha: Himantariidae; collected in Germany: Aachen, Greifswald. (8) Stigmatogaster dimidiatus (Meinert, 1870), Geophilomorpha: Himantariidae; collected in Spain: Ibiza.

Histology

For section series, several individuals were anesthetized, decapitated and prefixed for 24 h in a solution of 80% ethanol, 37% formaldehyde and 100% acetic acid (10:4:1). After washing in sodium hydrogen phosphate buffer (PBS, pH 7.4), specimens were postfixed for 1 h in 2% OsO4 solution (same buffer) at room temperature and, following dehydration in a graded series of acetone, embedded in Araldite (Araldite epoxy resin kit, Agar Scientific). Serial semithin sections (1-1.5 μm) were prepared with a Microm HM 355 S rotary microtome and stained using 1% toluidine blue and Pyronin G in a solution of 1% sodium tetraborate.

Autofluorescence preparation

For autofluorescence analysis, specimens were anesthetized and decapitated. Dissected brains were fixed in a solution of 4% paraformaldehyde and 4% glutaraldehyde (1:1) for at least one week at 4°C. After several washing steps in PBS, brains were dehydrated in a graded series of ethanol and embedded in methyl salycilate. For cLSM, an excitation of 488 nm was used to detect autofluorescence from the nervous tissue.

Antennal Backfilling

For antennal backfills, specimens were anesthetized and mounted in plastic Petri-dishes. One antenna was cut and the antennal nerve was exposed. For neurobiotin backfills, the antennal nerve stump was isolated in petroleum jelly, covered by aqua dest. for two minutes, and subsequently exposed to 5% neurobiotin (Vector Laboratories) being dissolved in aqua dest. Preparations were incubated at 4°C for 1 day. After final dissection and fixation in 4% paraformaldehyde for 24 hours, the preparations were washed in several changes of PBS and incubated in streptavidin conjugated to Cy3 (1:2000, Jackson Immunoresearch) for 24 hours. After washing in several changes of PBS, the preparations were dehydrated in a graded series of ethanol and mounted in methyl salycilate. In controls the brains of which were not subjected to backfills, incubation in streptavidin alone resulted in an absence of all labeling. For Lucifer yellow backfills, the treatment of the antennal nerve stump was the same as for neurobiotin backfills, but instead of washing and incubating, the preparations were directly dehydrated in a graded series of ethanol after fixation and mounted in methyl salycilate.

Microscopy, 3D reconstruction, and terminology

Wholemounts and brain sections were examined with a Nikon eclipse 90i microscope and a Leica SP 5 II confocal laser scanning microscope (cLSM). All images were processed with Adobe Photoshop using global contrast and brightness adjustment features.

The alignment and 3D reconstruction was made using AMIRA 5.1 (Visage Imaging) operated on a FS Celsius work station. In each section, contours of the nervous system and neuropilar regions were demarcated and a 3D reconstruction was generated. The 3D reconstruction of the brain of Craterostigmus tasmanianus was generated by merging two reconstructions of single brain hemispheres of the same specimen.

The neuroanatomical terminology is according to [30].

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Acknowledgements

The authors like to thank Robert Mesibov (Queen Victoria Museum and Art Gallery Launceston, Australia) and Gregory D. Edgecombe (Natural History Museum London, U.K.) for providing specimens of Craterostigmus tasmanianus. Our special thanks go to Jörg Rosenberg (Bergheim, Germany), Jakob Krieger (University of Greifswald, Germany), and Peter Bräunig (RWTH Aachen, Germany) for advice and inspiring discussions. This study was supported by DFG project HA 2540/8 and the Max Planck Society.

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    Authors and Affiliations

    1. Zoological Institute and Museum, Cytology and Evolutionary Biology, Ernst Moritz Arndt University of Greifswald, 17487, Greifswald, Germany

      Andy Sombke, Matthes Kenning, Carsten HG Müller & Steffen Harzsch

    2. Department of Evolutionary Neuroethology, Max Planck Institute for Chemical Ecology, 07745, Jena, Germany

      Andy Sombke, Bill S Hansson & Steffen Harzsch

    3. Zoological Institute and Museum, General Zoology and Zoological Systematics, Ernst Moritz Arndt University of Greifswald, 17487, Greifswald, Germany

      Elisabeth Lipke

    4. Institute of Biology II, Unit of Developmental Biology and Morphology of Animals, RWTH Aachen University, 52065, Aachen, Germany

      Elisabeth Lipke

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    1. Andy Sombke
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    Correspondence to Andy Sombke.

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    AS and EL conducted the sampling, preparation and fixation of brains, backfill experiments, and the 3D reconstructions. MK prepared the schematic representations of the chilopod brains. AS drafted the main part of the manuscript and all other authors assisted in drafting the manuscript. All authors read and approved the final manuscript.

    Bill S Hansson and Steffen Harzsch contributed equally to this work.

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    Sombke, A., Lipke, E., Kenning, M. et al. Comparative analysis of deutocerebral neuropils in Chilopoda (Myriapoda): implications for the evolution of the arthropod olfactory system and support for the Mandibulata concept. BMC Neurosci 13, 1 (2012). https://doi.org/10.1186/1471-2202-13-1

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